Fast spore breaking by superheatingElectronic supplementary information (ESI) available. See DOI: 10.1039/c3lc41305e

Fast spore breaking by superheatingElectronic supplementary information (ESI) available. See DOI: 10.1039/c3lc41305e

Here we present the results of Bacillus subtilis spores breaking using superheating. The spore sample was pumped through the open-ended capillary tube mounted across the heated zone. We investigated the influence of temperature in the range 120-180 °C. The heat exposure was controlled by the length...

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Hauptverfasser: P ibylka, Adam, Almeida, Ana Vanessa, Altmeyer, Matthias O, Petr, Jan, Šev ík, Juraj, Manz, Andreas, Neu il, Pavel
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Almeida, Ana Vanessa
Altmeyer, Matthias O
Petr, Jan
Šev ík, Juraj
Manz, Andreas
Neu il, Pavel
description Here we present the results of Bacillus subtilis spores breaking using superheating. The spore sample was pumped through the open-ended capillary tube mounted across the heated zone. We investigated the influence of temperature in the range 120-180 °C. The heat exposure was controlled by the length of the heated zone, the inner diameter of the capillary and the sample flow rate. We found that spore treatment above 120 °C resulted in the release of DNA within 20 s. We present an ultrafast flow through method of breaking Bacillus subtilis spores by superheating effect in micro capillaries.
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title Fast spore breaking by superheatingElectronic supplementary information (ESI) available. See DOI: 10.1039/c3lc41305e
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