Light-inducible activation of target mRNA translation in mammalian cellsElectronic supplementary information (ESI) available: Materials and methods, luciferase assay and DNA primer sequences. See DOI: 10.1039/c3cc44866e
A genetically encoded optogenetic system was constructed that activates mRNA translation in mammalian cells in response to light. Blue light induces the reconstitution of an RNA binding domain and a translation initiation domain, thereby activating target mRNA translation downstream of the binding s...
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| creator | Cao, Jicong Arha, Manish Sudrik, Chaitanya Bugaj, Lukasz J Schaffer, David V Kane, Ravi S |
| description | A genetically encoded optogenetic system was constructed that activates mRNA translation in mammalian cells in response to light. Blue light induces the reconstitution of an RNA binding domain and a translation initiation domain, thereby activating target mRNA translation downstream of the binding sites.
We have developed a light-inducible translational activation system in mammalian cells based on the light-sensitive reconstitution of an RNA binding domain and a translation initiation domain. |
| doi_str_mv | 10.1039/c3cc44866e |
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| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| title | Light-inducible activation of target mRNA translation in mammalian cellsElectronic supplementary information (ESI) available: Materials and methods, luciferase assay and DNA primer sequences. See DOI: 10.1039/c3cc44866e |
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