Hemin/G-quadruplex simultaneously acts as NADH oxidase and HRP-mimicking DNAzyme for simple, sensitive pseudobienzyme electrochemical detection of thrombinElectronic supplementary information (ESI) available: Analytical properties of hemin/G-quadruplex assembled aptasensors for thrombin detection, experimental section, hemin/G-quadruplex electrocatalyst kinetics, the amplification of AuTHAu nanoparticles, optimization of detection conditions, and comparisons of proposed aptasensors with other de

For the first time, hemin/G-quadruplex was employed to simultaneously serve as NADH oxidase and an HRP-mimicking DNAzyme for constructing a simple and sensitive pseudobienzyme-amplifying electrochemical aptasensor for thrombin detection. Illustration of the stepwise preparation of a pseudobienzyme electrochemical aptasensor and the bioelectrocatalytic principle of hemin/G-quadruplex structure.