Characterisation of PigC and HapC, the prodigiosin synthetases from Serratia sp. and Hahella chejuensis with potential for biocatalytic production of anticancer agentsElectronic supplementary information (ESI) available: Full experimental details, protein gels, MichaelisMenten plots for PigC and HapC with all the MBC analogues, details of the kinetic models used to explain the substrate inhibition by MAP. Sequence alignment of PigC and HapC with PEPS and PPDK and homology model for PigC superimp

Characterisation of PigC and HapC, the prodigiosin synthetases from Serratia sp. and Hahella chejuensis with potential for biocatalytic production of anticancer agentsElectronic supplementary information (ESI) available: Full experimental details, protein gels, MichaelisMenten plots for PigC and HapC with all the MBC analogues, details of the kinetic models used to explain the substrate inhibition by MAP. Sequence alignment of PigC and HapC with PEPS and PPDK and homology model for PigC superimp

PigC and HapC catalyse the condensation of 4-methoxy-2,2-bipyrrole-5-carbaldehyde (MBC) with 2-methyl-3-amylpyrrole (MAP) to give the bright red tripyrrolic prodigiosin, which has potent anticancer activity. We have cloned and over-expressed both enzymes and characterised their enzymic activity in v...

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Hauptverfasser: Chawrai, Suresh R, Williamson, Neil R, Mahendiran, Thabo, Salmond, George P. C, Leeper, Finian J
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Sprache:eng
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