Competitive surface-enhanced Raman scattering assay for the 1,25-dihydroxy metabolite of vitamin D3
This paper describes the development and preliminary testing of a competitive surface-enhanced Raman scattering (SERS) immunoassay for calcitriol, the 1,25-dihydroxy metabolite (1,25-(OH) 2 -D 3 ) of vitamin D 3 . Deficiencies in 1,25-(OH) 2 -D have been linked to renal disease, while elevations are...
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| Veröffentlicht in: | Analyst (London) 2010-11, Vol.135 (11), p.2811-2817 |
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| creator | Dufek, Eric J Ehlert, Brian Granger, Michael C Sandrock, Tanya M Legge, Samuel L Herrmann, Mark G Meikle, A. Wayne Porter, Marc D |
| description | This paper describes the development and preliminary testing of a competitive surface-enhanced Raman scattering (SERS) immunoassay for calcitriol, the 1,25-dihydroxy metabolite (1,25-(OH)
2
-D
3
) of vitamin D
3
. Deficiencies in 1,25-(OH)
2
-D have been linked to renal disease, while elevations are linked to hypercalcemia. Thus, there has been a sharp increase in the clinical demand for measurements of this metabolite. The work herein extends the many attributes of SERS-based sandwich immunoassays that have been exploited extensively in the detection of large biolytes (
e.g.
, DNA, proteins, viruses, and microorganisms) into a competitive immunoassay for the low level determination of a small biolyte, 1,25-(OH)
2
-D
3
(
M
w
= 416 g mol
−1
). The assay uses surface modified gold nanoparticles as SERS labels, and has a dynamic range of 10-200 pg mL
−1
and a limit of detection of 8.4 ± 1.8 pg mL
−1
. These analytical performance metrics match those of tests for 1,25-(OH)
2
-D
3
that rely on radio- or enzyme-labels, while using a much smaller sample volume and eliminating the disposal of radioactive wastes. Moreover, the SERS-based data from pooled-patient sera show strong agreement with that from radioimmunoassays. The merits and potential utility of this new assay are briefly discussed.
This article highlights the development of a competitive surface-enhanced Raman scattering immunoassay for detection and quantification of 1,25-dihydroxy vitamin D
3
. |
| doi_str_mv | 10.1039/c0an00354a |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_rsc_p</sourceid><recordid>TN_cdi_rsc_primary_c0an00354a</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>759324463</sourcerecordid><originalsourceid>FETCH-LOGICAL-c279t-7f876359aaf35e9bb58818bd0958351c629d98b04dc76652262ca7767ce9024a3</originalsourceid><addsrcrecordid>eNp90M9LHDEUwPEglbq1vXhviYciiKP5PZOjbOsPEAqi5-FN5sVNmR_bJCvuf-_IroqXnkLyPrzAl5ADzk45k_bMMRgYk1rBDplxaVShtag-kRmbXgthtNojX1L6O1050-wz2ROskkwKPSNuPvZLzCGHR6RpFT04LHBYwOCwpbfQw0CTg5wxhuGBQkqwpn6MNC-Q8hOhizYs1m0cn9a0xwzN2IWMdPT0MWTow0B_ya9k10OX8Nv23Cf3F7_v5lfFzZ_L6_n5TeFEaXNR-qo0UlsALzXaptFVxaumZVZXUnNnhG1t1TDVutIYLYQRDsrSlA4tEwrkPjna7F3G8d8KU677kBx2HQw4rlJdaiuFUkZO8ngjXRxTiujrZQw9xHXNWf3StH5vOuEf27Wrpsf2jb5GnMDPLYApVefjFC-kdycVN0LxyX3fuJjc2_TDR4f_m9fL1stn4SmS4Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>759324463</pqid></control><display><type>article</type><title>Competitive surface-enhanced Raman scattering assay for the 1,25-dihydroxy metabolite of vitamin D3</title><source>MEDLINE</source><source>Royal Society of Chemistry Journals Archive (1841-2007)</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Dufek, Eric J ; Ehlert, Brian ; Granger, Michael C ; Sandrock, Tanya M ; Legge, Samuel L ; Herrmann, Mark G ; Meikle, A. Wayne ; Porter, Marc D</creator><creatorcontrib>Dufek, Eric J ; Ehlert, Brian ; Granger, Michael C ; Sandrock, Tanya M ; Legge, Samuel L ; Herrmann, Mark G ; Meikle, A. Wayne ; Porter, Marc D</creatorcontrib><description>This paper describes the development and preliminary testing of a competitive surface-enhanced Raman scattering (SERS) immunoassay for calcitriol, the 1,25-dihydroxy metabolite (1,25-(OH)
2
-D
3
) of vitamin D
3
. Deficiencies in 1,25-(OH)
2
-D have been linked to renal disease, while elevations are linked to hypercalcemia. Thus, there has been a sharp increase in the clinical demand for measurements of this metabolite. The work herein extends the many attributes of SERS-based sandwich immunoassays that have been exploited extensively in the detection of large biolytes (
e.g.
, DNA, proteins, viruses, and microorganisms) into a competitive immunoassay for the low level determination of a small biolyte, 1,25-(OH)
2
-D
3
(
M
w
= 416 g mol
−1
). The assay uses surface modified gold nanoparticles as SERS labels, and has a dynamic range of 10-200 pg mL
−1
and a limit of detection of 8.4 ± 1.8 pg mL
−1
. These analytical performance metrics match those of tests for 1,25-(OH)
2
-D
3
that rely on radio- or enzyme-labels, while using a much smaller sample volume and eliminating the disposal of radioactive wastes. Moreover, the SERS-based data from pooled-patient sera show strong agreement with that from radioimmunoassays. The merits and potential utility of this new assay are briefly discussed.
This article highlights the development of a competitive surface-enhanced Raman scattering immunoassay for detection and quantification of 1,25-dihydroxy vitamin D
3
.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c0an00354a</identifier><identifier>PMID: 20830325</identifier><identifier>CODEN: ANALAO</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Analysis methods ; Analytical chemistry ; Applied sciences ; Calcitriol - analogs & derivatives ; Calcitriol - analysis ; Calcitriol - metabolism ; Chemistry ; Exact sciences and technology ; Humans ; Immunoassay ; Miscellaneous ; Molecular Structure ; Pollution ; Spectrometric and optical methods ; Spectrum Analysis, Raman - methods ; Surface Properties ; Wastes</subject><ispartof>Analyst (London), 2010-11, Vol.135 (11), p.2811-2817</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c279t-7f876359aaf35e9bb58818bd0958351c629d98b04dc76652262ca7767ce9024a3</citedby><cites>FETCH-LOGICAL-c279t-7f876359aaf35e9bb58818bd0958351c629d98b04dc76652262ca7767ce9024a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,2818,2819,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23416241$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20830325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dufek, Eric J</creatorcontrib><creatorcontrib>Ehlert, Brian</creatorcontrib><creatorcontrib>Granger, Michael C</creatorcontrib><creatorcontrib>Sandrock, Tanya M</creatorcontrib><creatorcontrib>Legge, Samuel L</creatorcontrib><creatorcontrib>Herrmann, Mark G</creatorcontrib><creatorcontrib>Meikle, A. Wayne</creatorcontrib><creatorcontrib>Porter, Marc D</creatorcontrib><title>Competitive surface-enhanced Raman scattering assay for the 1,25-dihydroxy metabolite of vitamin D3</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>This paper describes the development and preliminary testing of a competitive surface-enhanced Raman scattering (SERS) immunoassay for calcitriol, the 1,25-dihydroxy metabolite (1,25-(OH)
2
-D
3
) of vitamin D
3
. Deficiencies in 1,25-(OH)
2
-D have been linked to renal disease, while elevations are linked to hypercalcemia. Thus, there has been a sharp increase in the clinical demand for measurements of this metabolite. The work herein extends the many attributes of SERS-based sandwich immunoassays that have been exploited extensively in the detection of large biolytes (
e.g.
, DNA, proteins, viruses, and microorganisms) into a competitive immunoassay for the low level determination of a small biolyte, 1,25-(OH)
2
-D
3
(
M
w
= 416 g mol
−1
). The assay uses surface modified gold nanoparticles as SERS labels, and has a dynamic range of 10-200 pg mL
−1
and a limit of detection of 8.4 ± 1.8 pg mL
−1
. These analytical performance metrics match those of tests for 1,25-(OH)
2
-D
3
that rely on radio- or enzyme-labels, while using a much smaller sample volume and eliminating the disposal of radioactive wastes. Moreover, the SERS-based data from pooled-patient sera show strong agreement with that from radioimmunoassays. The merits and potential utility of this new assay are briefly discussed.
This article highlights the development of a competitive surface-enhanced Raman scattering immunoassay for detection and quantification of 1,25-dihydroxy vitamin D
3
.</description><subject>Analysis methods</subject><subject>Analytical chemistry</subject><subject>Applied sciences</subject><subject>Calcitriol - analogs & derivatives</subject><subject>Calcitriol - analysis</subject><subject>Calcitriol - metabolism</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Miscellaneous</subject><subject>Molecular Structure</subject><subject>Pollution</subject><subject>Spectrometric and optical methods</subject><subject>Spectrum Analysis, Raman - methods</subject><subject>Surface Properties</subject><subject>Wastes</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90M9LHDEUwPEglbq1vXhviYciiKP5PZOjbOsPEAqi5-FN5sVNmR_bJCvuf-_IroqXnkLyPrzAl5ADzk45k_bMMRgYk1rBDplxaVShtag-kRmbXgthtNojX1L6O1050-wz2ROskkwKPSNuPvZLzCGHR6RpFT04LHBYwOCwpbfQw0CTg5wxhuGBQkqwpn6MNC-Q8hOhizYs1m0cn9a0xwzN2IWMdPT0MWTow0B_ya9k10OX8Nv23Cf3F7_v5lfFzZ_L6_n5TeFEaXNR-qo0UlsALzXaptFVxaumZVZXUnNnhG1t1TDVutIYLYQRDsrSlA4tEwrkPjna7F3G8d8KU677kBx2HQw4rlJdaiuFUkZO8ngjXRxTiujrZQw9xHXNWf3StH5vOuEf27Wrpsf2jb5GnMDPLYApVefjFC-kdycVN0LxyX3fuJjc2_TDR4f_m9fL1stn4SmS4Q</recordid><startdate>201011</startdate><enddate>201011</enddate><creator>Dufek, Eric J</creator><creator>Ehlert, Brian</creator><creator>Granger, Michael C</creator><creator>Sandrock, Tanya M</creator><creator>Legge, Samuel L</creator><creator>Herrmann, Mark G</creator><creator>Meikle, A. Wayne</creator><creator>Porter, Marc D</creator><general>Royal Society of Chemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201011</creationdate><title>Competitive surface-enhanced Raman scattering assay for the 1,25-dihydroxy metabolite of vitamin D3</title><author>Dufek, Eric J ; Ehlert, Brian ; Granger, Michael C ; Sandrock, Tanya M ; Legge, Samuel L ; Herrmann, Mark G ; Meikle, A. Wayne ; Porter, Marc D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c279t-7f876359aaf35e9bb58818bd0958351c629d98b04dc76652262ca7767ce9024a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analysis methods</topic><topic>Analytical chemistry</topic><topic>Applied sciences</topic><topic>Calcitriol - analogs & derivatives</topic><topic>Calcitriol - analysis</topic><topic>Calcitriol - metabolism</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Miscellaneous</topic><topic>Molecular Structure</topic><topic>Pollution</topic><topic>Spectrometric and optical methods</topic><topic>Spectrum Analysis, Raman - methods</topic><topic>Surface Properties</topic><topic>Wastes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dufek, Eric J</creatorcontrib><creatorcontrib>Ehlert, Brian</creatorcontrib><creatorcontrib>Granger, Michael C</creatorcontrib><creatorcontrib>Sandrock, Tanya M</creatorcontrib><creatorcontrib>Legge, Samuel L</creatorcontrib><creatorcontrib>Herrmann, Mark G</creatorcontrib><creatorcontrib>Meikle, A. Wayne</creatorcontrib><creatorcontrib>Porter, Marc D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dufek, Eric J</au><au>Ehlert, Brian</au><au>Granger, Michael C</au><au>Sandrock, Tanya M</au><au>Legge, Samuel L</au><au>Herrmann, Mark G</au><au>Meikle, A. Wayne</au><au>Porter, Marc D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Competitive surface-enhanced Raman scattering assay for the 1,25-dihydroxy metabolite of vitamin D3</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2010-11</date><risdate>2010</risdate><volume>135</volume><issue>11</issue><spage>2811</spage><epage>2817</epage><pages>2811-2817</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><coden>ANALAO</coden><abstract>This paper describes the development and preliminary testing of a competitive surface-enhanced Raman scattering (SERS) immunoassay for calcitriol, the 1,25-dihydroxy metabolite (1,25-(OH)
2
-D
3
) of vitamin D
3
. Deficiencies in 1,25-(OH)
2
-D have been linked to renal disease, while elevations are linked to hypercalcemia. Thus, there has been a sharp increase in the clinical demand for measurements of this metabolite. The work herein extends the many attributes of SERS-based sandwich immunoassays that have been exploited extensively in the detection of large biolytes (
e.g.
, DNA, proteins, viruses, and microorganisms) into a competitive immunoassay for the low level determination of a small biolyte, 1,25-(OH)
2
-D
3
(
M
w
= 416 g mol
−1
). The assay uses surface modified gold nanoparticles as SERS labels, and has a dynamic range of 10-200 pg mL
−1
and a limit of detection of 8.4 ± 1.8 pg mL
−1
. These analytical performance metrics match those of tests for 1,25-(OH)
2
-D
3
that rely on radio- or enzyme-labels, while using a much smaller sample volume and eliminating the disposal of radioactive wastes. Moreover, the SERS-based data from pooled-patient sera show strong agreement with that from radioimmunoassays. The merits and potential utility of this new assay are briefly discussed.
This article highlights the development of a competitive surface-enhanced Raman scattering immunoassay for detection and quantification of 1,25-dihydroxy vitamin D
3
.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><pmid>20830325</pmid><doi>10.1039/c0an00354a</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Analysis methods Analytical chemistry Applied sciences Calcitriol - analogs & derivatives Calcitriol - analysis Calcitriol - metabolism Chemistry Exact sciences and technology Humans Immunoassay Miscellaneous Molecular Structure Pollution Spectrometric and optical methods Spectrum Analysis, Raman - methods Surface Properties Wastes |
| title | Competitive surface-enhanced Raman scattering assay for the 1,25-dihydroxy metabolite of vitamin D3 |
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