Prions and the lymphoreticular system
Following intracerebral or peripheral inoculation of mice with scrapie prions, infectivity accumulates first in the spleen and only later in the brain. In the spleen of scrapie-infected mice, prions were found in association with T and B lymphocytes and to a somewhat lesser degree with the stroma, w...
Gespeichert in:
Veröffentlicht in: | Philosophical transactions of the Royal Society of London. Series B. Biological sciences 2001-02, Vol.356 (1406), p.177-184 |
---|---|
Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 184 |
---|---|
container_issue | 1406 |
container_start_page | 177 |
container_title | Philosophical transactions of the Royal Society of London. Series B. Biological sciences |
container_volume | 356 |
creator | Dobson, C. M. Ellis, R. J. Fersht, A. R. Weissmann, Charles Raeber, Alex J. Montrasio, Fabio Hegyi, Ivan Frigg, Rico Klein, Michael A. Aguzzi, Adriano |
description | Following intracerebral or peripheral inoculation of mice with scrapie prions, infectivity accumulates first in the spleen and only later in the brain. In the spleen of scrapie-infected mice, prions were found in association with T and B lymphocytes and to a somewhat lesser degree with the stroma, which contains the follicular dendritic cells (FDCs) but not with non-B, non-T cells; strikingly, no infectivity was found in lymphocytes from blood of the same mice. Transgenic PrP knockout mice expressing PrP restricted to either B or T lymphocytes show no prion replication in the lymphoreticular system. Therefore, splenic lymphocytes either acquire prions from another source or replicate them in dependency on other PrP-expressing cells. The essential role of FDCs in prion replication in spleen was shown by treating mice with soluble lymphotoxin-β receptor, which led to disappearance of mature FDCs from the spleen and concomitantly abolished splenic prion accumulation and retarded neuroinvasion following intraperitoneal scrapie inoculation. |
doi_str_mv | 10.1098/rstb.2000.0763 |
format | Article |
fullrecord | <record><control><sourceid>jstor_royal</sourceid><recordid>TN_cdi_royalsociety_journals_10_1098_rstb_2000_0763</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>3067086</jstor_id><sourcerecordid>3067086</sourcerecordid><originalsourceid>FETCH-LOGICAL-c532t-5963b178c19c90cfbb8a547f29fdd6dc6919b441fbf37505280dbb5256161ff83</originalsourceid><addsrcrecordid>eNp1kb2P1DAQxS0E4paDlgqhbbgui78_GiR04gCxEhQHrRU79sVLEi92AuS_x1FWC1fQ2Bq937wZvQHgOYI7BJV8nfJodhhCuIOCkwdgg6hAFVYCPgQbqDiuJCX8AjzJ-VAoxQR9DC4QwhwKJTfg1ZcU4pC39dBsx9Ztu7k_tjG5Mdipq9M2z3l0_VPwyNddds9O_yX4evPu9vpDtf_8_uP1231lGcFjxRQnBglpkbIKWm-MrBkVHivfNLyxXCFlKEXeeCIYZFjCxhiGGUcceS_JJXiz-h4n07vGumFMdaePKfR1mnWsg76vDKHVd_GnRlBKikkxuDoZpPhjcnnUfcjWdV09uDhlLbiSCglVwN0K2hRzTs6fhyCol2T1kqxektVLsqXh5b-r_cVPURaArECKc8ko2uDGWR_ilIZS_t_2xdp1yGNMZ1cCuYCSF7la5VDO8Pss1-m75qJkqL9Jqim6_aTIHumFhyvfhrv2V0hO39umFMcynjCuEYXlEYL8AS-9r7c</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76989179</pqid></control><display><type>article</type><title>Prions and the lymphoreticular system</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><source>PubMed Central</source><creator>Dobson, C. M. ; Ellis, R. J. ; Fersht, A. R. ; Weissmann, Charles ; Raeber, Alex J. ; Montrasio, Fabio ; Hegyi, Ivan ; Frigg, Rico ; Klein, Michael A. ; Aguzzi, Adriano</creator><contributor>Dobson, C. M. ; Ellis, R. J. ; Fersht, A. R. ; Dobson, C. M. ; Fersht, A. R. ; Ellis, R. J.</contributor><creatorcontrib>Dobson, C. M. ; Ellis, R. J. ; Fersht, A. R. ; Weissmann, Charles ; Raeber, Alex J. ; Montrasio, Fabio ; Hegyi, Ivan ; Frigg, Rico ; Klein, Michael A. ; Aguzzi, Adriano ; Dobson, C. M. ; Ellis, R. J. ; Fersht, A. R. ; Dobson, C. M. ; Fersht, A. R. ; Ellis, R. J.</creatorcontrib><description>Following intracerebral or peripheral inoculation of mice with scrapie prions, infectivity accumulates first in the spleen and only later in the brain. In the spleen of scrapie-infected mice, prions were found in association with T and B lymphocytes and to a somewhat lesser degree with the stroma, which contains the follicular dendritic cells (FDCs) but not with non-B, non-T cells; strikingly, no infectivity was found in lymphocytes from blood of the same mice. Transgenic PrP knockout mice expressing PrP restricted to either B or T lymphocytes show no prion replication in the lymphoreticular system. Therefore, splenic lymphocytes either acquire prions from another source or replicate them in dependency on other PrP-expressing cells. The essential role of FDCs in prion replication in spleen was shown by treating mice with soluble lymphotoxin-β receptor, which led to disappearance of mature FDCs from the spleen and concomitantly abolished splenic prion accumulation and retarded neuroinvasion following intraperitoneal scrapie inoculation.</description><identifier>ISSN: 0962-8436</identifier><identifier>EISSN: 1471-2970</identifier><identifier>DOI: 10.1098/rstb.2000.0763</identifier><identifier>PMID: 11260798</identifier><language>eng</language><publisher>England: The Royal Society</publisher><subject>Animals ; B Lymphocytes ; Central nervous system ; Creutzfeldt Jakob syndrome ; Dendritic Cells ; Ectopic Expression ; Follicular Dendritic Cells ; Humans ; Infections ; Inoculation ; Lymphatic System - physiology ; Lymphocytes ; Lymphotoxin ; Mice ; Mice, Knockout ; Mononuclear Phagocyte System - physiology ; Peptide Fragments - genetics ; Prion diseases ; Prions ; Prions - genetics ; Prions - metabolism ; PrP ; Spleen ; Spleen - metabolism ; T lymphocytes</subject><ispartof>Philosophical transactions of the Royal Society of London. Series B. Biological sciences, 2001-02, Vol.356 (1406), p.177-184</ispartof><rights>Copyright 2001 The Royal Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-5963b178c19c90cfbb8a547f29fdd6dc6919b441fbf37505280dbb5256161ff83</citedby><cites>FETCH-LOGICAL-c532t-5963b178c19c90cfbb8a547f29fdd6dc6919b441fbf37505280dbb5256161ff83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3067086$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3067086$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11260798$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Dobson, C. M.</contributor><contributor>Ellis, R. J.</contributor><contributor>Fersht, A. R.</contributor><contributor>Dobson, C. M.</contributor><contributor>Fersht, A. R.</contributor><contributor>Ellis, R. J.</contributor><creatorcontrib>Dobson, C. M.</creatorcontrib><creatorcontrib>Ellis, R. J.</creatorcontrib><creatorcontrib>Fersht, A. R.</creatorcontrib><creatorcontrib>Weissmann, Charles</creatorcontrib><creatorcontrib>Raeber, Alex J.</creatorcontrib><creatorcontrib>Montrasio, Fabio</creatorcontrib><creatorcontrib>Hegyi, Ivan</creatorcontrib><creatorcontrib>Frigg, Rico</creatorcontrib><creatorcontrib>Klein, Michael A.</creatorcontrib><creatorcontrib>Aguzzi, Adriano</creatorcontrib><title>Prions and the lymphoreticular system</title><title>Philosophical transactions of the Royal Society of London. Series B. Biological sciences</title><addtitle>Philos Trans R Soc Lond B Biol Sci</addtitle><description>Following intracerebral or peripheral inoculation of mice with scrapie prions, infectivity accumulates first in the spleen and only later in the brain. In the spleen of scrapie-infected mice, prions were found in association with T and B lymphocytes and to a somewhat lesser degree with the stroma, which contains the follicular dendritic cells (FDCs) but not with non-B, non-T cells; strikingly, no infectivity was found in lymphocytes from blood of the same mice. Transgenic PrP knockout mice expressing PrP restricted to either B or T lymphocytes show no prion replication in the lymphoreticular system. Therefore, splenic lymphocytes either acquire prions from another source or replicate them in dependency on other PrP-expressing cells. The essential role of FDCs in prion replication in spleen was shown by treating mice with soluble lymphotoxin-β receptor, which led to disappearance of mature FDCs from the spleen and concomitantly abolished splenic prion accumulation and retarded neuroinvasion following intraperitoneal scrapie inoculation.</description><subject>Animals</subject><subject>B Lymphocytes</subject><subject>Central nervous system</subject><subject>Creutzfeldt Jakob syndrome</subject><subject>Dendritic Cells</subject><subject>Ectopic Expression</subject><subject>Follicular Dendritic Cells</subject><subject>Humans</subject><subject>Infections</subject><subject>Inoculation</subject><subject>Lymphatic System - physiology</subject><subject>Lymphocytes</subject><subject>Lymphotoxin</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Mononuclear Phagocyte System - physiology</subject><subject>Peptide Fragments - genetics</subject><subject>Prion diseases</subject><subject>Prions</subject><subject>Prions - genetics</subject><subject>Prions - metabolism</subject><subject>PrP</subject><subject>Spleen</subject><subject>Spleen - metabolism</subject><subject>T lymphocytes</subject><issn>0962-8436</issn><issn>1471-2970</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kb2P1DAQxS0E4paDlgqhbbgui78_GiR04gCxEhQHrRU79sVLEi92AuS_x1FWC1fQ2Bq937wZvQHgOYI7BJV8nfJodhhCuIOCkwdgg6hAFVYCPgQbqDiuJCX8AjzJ-VAoxQR9DC4QwhwKJTfg1ZcU4pC39dBsx9Ztu7k_tjG5Mdipq9M2z3l0_VPwyNddds9O_yX4evPu9vpDtf_8_uP1231lGcFjxRQnBglpkbIKWm-MrBkVHivfNLyxXCFlKEXeeCIYZFjCxhiGGUcceS_JJXiz-h4n07vGumFMdaePKfR1mnWsg76vDKHVd_GnRlBKikkxuDoZpPhjcnnUfcjWdV09uDhlLbiSCglVwN0K2hRzTs6fhyCol2T1kqxektVLsqXh5b-r_cVPURaArECKc8ko2uDGWR_ilIZS_t_2xdp1yGNMZ1cCuYCSF7la5VDO8Pss1-m75qJkqL9Jqim6_aTIHumFhyvfhrv2V0hO39umFMcynjCuEYXlEYL8AS-9r7c</recordid><startdate>20010228</startdate><enddate>20010228</enddate><creator>Dobson, C. M.</creator><creator>Ellis, R. J.</creator><creator>Fersht, A. R.</creator><creator>Weissmann, Charles</creator><creator>Raeber, Alex J.</creator><creator>Montrasio, Fabio</creator><creator>Hegyi, Ivan</creator><creator>Frigg, Rico</creator><creator>Klein, Michael A.</creator><creator>Aguzzi, Adriano</creator><general>The Royal Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20010228</creationdate><title>Prions and the lymphoreticular system</title><author>Dobson, C. M. ; Ellis, R. J. ; Fersht, A. R. ; Weissmann, Charles ; Raeber, Alex J. ; Montrasio, Fabio ; Hegyi, Ivan ; Frigg, Rico ; Klein, Michael A. ; Aguzzi, Adriano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-5963b178c19c90cfbb8a547f29fdd6dc6919b441fbf37505280dbb5256161ff83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>B Lymphocytes</topic><topic>Central nervous system</topic><topic>Creutzfeldt Jakob syndrome</topic><topic>Dendritic Cells</topic><topic>Ectopic Expression</topic><topic>Follicular Dendritic Cells</topic><topic>Humans</topic><topic>Infections</topic><topic>Inoculation</topic><topic>Lymphatic System - physiology</topic><topic>Lymphocytes</topic><topic>Lymphotoxin</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Mononuclear Phagocyte System - physiology</topic><topic>Peptide Fragments - genetics</topic><topic>Prion diseases</topic><topic>Prions</topic><topic>Prions - genetics</topic><topic>Prions - metabolism</topic><topic>PrP</topic><topic>Spleen</topic><topic>Spleen - metabolism</topic><topic>T lymphocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dobson, C. M.</creatorcontrib><creatorcontrib>Ellis, R. J.</creatorcontrib><creatorcontrib>Fersht, A. R.</creatorcontrib><creatorcontrib>Weissmann, Charles</creatorcontrib><creatorcontrib>Raeber, Alex J.</creatorcontrib><creatorcontrib>Montrasio, Fabio</creatorcontrib><creatorcontrib>Hegyi, Ivan</creatorcontrib><creatorcontrib>Frigg, Rico</creatorcontrib><creatorcontrib>Klein, Michael A.</creatorcontrib><creatorcontrib>Aguzzi, Adriano</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Philosophical transactions of the Royal Society of London. Series B. Biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dobson, C. M.</au><au>Ellis, R. J.</au><au>Fersht, A. R.</au><au>Weissmann, Charles</au><au>Raeber, Alex J.</au><au>Montrasio, Fabio</au><au>Hegyi, Ivan</au><au>Frigg, Rico</au><au>Klein, Michael A.</au><au>Aguzzi, Adriano</au><au>Dobson, C. M.</au><au>Ellis, R. J.</au><au>Fersht, A. R.</au><au>Dobson, C. M.</au><au>Fersht, A. R.</au><au>Ellis, R. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prions and the lymphoreticular system</atitle><jtitle>Philosophical transactions of the Royal Society of London. Series B. Biological sciences</jtitle><addtitle>Philos Trans R Soc Lond B Biol Sci</addtitle><date>2001-02-28</date><risdate>2001</risdate><volume>356</volume><issue>1406</issue><spage>177</spage><epage>184</epage><pages>177-184</pages><issn>0962-8436</issn><eissn>1471-2970</eissn><abstract>Following intracerebral or peripheral inoculation of mice with scrapie prions, infectivity accumulates first in the spleen and only later in the brain. In the spleen of scrapie-infected mice, prions were found in association with T and B lymphocytes and to a somewhat lesser degree with the stroma, which contains the follicular dendritic cells (FDCs) but not with non-B, non-T cells; strikingly, no infectivity was found in lymphocytes from blood of the same mice. Transgenic PrP knockout mice expressing PrP restricted to either B or T lymphocytes show no prion replication in the lymphoreticular system. Therefore, splenic lymphocytes either acquire prions from another source or replicate them in dependency on other PrP-expressing cells. The essential role of FDCs in prion replication in spleen was shown by treating mice with soluble lymphotoxin-β receptor, which led to disappearance of mature FDCs from the spleen and concomitantly abolished splenic prion accumulation and retarded neuroinvasion following intraperitoneal scrapie inoculation.</abstract><cop>England</cop><pub>The Royal Society</pub><pmid>11260798</pmid><doi>10.1098/rstb.2000.0763</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0962-8436 |
ispartof | Philosophical transactions of the Royal Society of London. Series B. Biological sciences, 2001-02, Vol.356 (1406), p.177-184 |
issn | 0962-8436 1471-2970 |
language | eng |
recordid | cdi_royalsociety_journals_10_1098_rstb_2000_0763 |
source | Jstor Complete Legacy; MEDLINE; PubMed Central |
subjects | Animals B Lymphocytes Central nervous system Creutzfeldt Jakob syndrome Dendritic Cells Ectopic Expression Follicular Dendritic Cells Humans Infections Inoculation Lymphatic System - physiology Lymphocytes Lymphotoxin Mice Mice, Knockout Mononuclear Phagocyte System - physiology Peptide Fragments - genetics Prion diseases Prions Prions - genetics Prions - metabolism PrP Spleen Spleen - metabolism T lymphocytes |
title | Prions and the lymphoreticular system |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T17%3A18%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_royal&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Prions%20and%20the%20lymphoreticular%20system&rft.jtitle=Philosophical%20transactions%20of%20the%20Royal%20Society%20of%20London.%20Series%20B.%20Biological%20sciences&rft.au=Dobson,%20C.%20M.&rft.date=2001-02-28&rft.volume=356&rft.issue=1406&rft.spage=177&rft.epage=184&rft.pages=177-184&rft.issn=0962-8436&rft.eissn=1471-2970&rft_id=info:doi/10.1098/rstb.2000.0763&rft_dat=%3Cjstor_royal%3E3067086%3C/jstor_royal%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76989179&rft_id=info:pmid/11260798&rft_jstor_id=3067086&rfr_iscdi=true |