Increased Anion Permeability During Volume Regulation in Human Lymphocytes
Peripheral blood lymphocytes (p.b.ls) readjust their volumes after swelling in hypotonic media. An essential component of the regulatory response is an increase in K$^+$ and Cl$^-$ permeability. No evidence was found for a tightly coupled co-transport of K$^+$ and Cl$^-$. The flux of either ion proc...
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Veröffentlicht in: | Philosophical transactions of the Royal Society of London. Series B, Biological sciences Biological sciences, 1982-12, Vol.299 (1097), p.509-518 |
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creator | Grinstein, S. Clarke, C. A. Rothstein, A. |
description | Peripheral blood lymphocytes (p.b.ls) readjust their volumes after swelling in hypotonic media. An essential component of
the regulatory response is an increase in K$^+$ and Cl$^-$ permeability. No evidence was found
for a tightly coupled co-transport of K$^+$ and Cl$^-$. The flux of either ion proceeds normally
in the virtual absence of the transported counterion. Furthermore, alterations in membrane potential recorded during the phase
of volume readjustment can be qualitatively accounted for by an increase in Cl$^-$ conductance. In tonsillar
lymphocytes, a failure of the K$^+$-permeation path to respond to swelling leads to deficient volume recovery,
but Cl$^-$ permeability is nevertheless increased upon swelling. This further suggests that K$^+$
and Cl$^-$ are transported during volume regulation through independent pathways. Cytoplasmic free Ca$^{2+}$
appears to be involved in regulatory volume decrease. K$^+$ and Cl$^-$ fluxes similar to those
elicited by swelling can also be produced by A23187 plus Ca$^{2+}$. Moreover, swelling and shrinking can be
induced in isotonic K$^+$-rich and K$^+$-free media, respectively, by the Ca$^{2+}$
ionophore. The ion flux and volume changes produced by either swelling or internal Ca$^{2+}$ can be inhibited
by similar concentrations of quinine and phenothiazines. The inhibitory activity of the latter drugs, which are powerful antagonists
of calmodulin, suggests the participation of this Ca$^{2+}$-regulator protein in volume regulation. |
doi_str_mv | 10.1098/rstb.1982.0148 |
format | Article |
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the regulatory response is an increase in K$^+$ and Cl$^-$ permeability. No evidence was found
for a tightly coupled co-transport of K$^+$ and Cl$^-$. The flux of either ion proceeds normally
in the virtual absence of the transported counterion. Furthermore, alterations in membrane potential recorded during the phase
of volume readjustment can be qualitatively accounted for by an increase in Cl$^-$ conductance. In tonsillar
lymphocytes, a failure of the K$^+$-permeation path to respond to swelling leads to deficient volume recovery,
but Cl$^-$ permeability is nevertheless increased upon swelling. This further suggests that K$^+$
and Cl$^-$ are transported during volume regulation through independent pathways. Cytoplasmic free Ca$^{2+}$
appears to be involved in regulatory volume decrease. K$^+$ and Cl$^-$ fluxes similar to those
elicited by swelling can also be produced by A23187 plus Ca$^{2+}$. Moreover, swelling and shrinking can be
induced in isotonic K$^+$-rich and K$^+$-free media, respectively, by the Ca$^{2+}$
ionophore. The ion flux and volume changes produced by either swelling or internal Ca$^{2+}$ can be inhibited
by similar concentrations of quinine and phenothiazines. The inhibitory activity of the latter drugs, which are powerful antagonists
of calmodulin, suggests the participation of this Ca$^{2+}$-regulator protein in volume regulation.</description><identifier>ISSN: 0962-8436</identifier><identifier>ISSN: 0080-4622</identifier><identifier>EISSN: 1471-2970</identifier><identifier>EISSN: 2054-0280</identifier><identifier>DOI: 10.1098/rstb.1982.0148</identifier><identifier>PMID: 6130543</identifier><language>eng</language><publisher>London: The Royal Society</publisher><subject>Anions ; Anions - physiology ; B lymphocytes ; Calcium - physiology ; Cations ; Cell Membrane Permeability ; Chloride Exchange and Co-Transport Mechanisms ; Chlorides - physiology ; Drug regulation ; Erythrocytes ; Gluconates ; Humans ; Ion Channels - physiology ; Lymphocytes ; Lymphocytes - cytology ; Lymphocytes - physiology ; Membrane potential ; Osmolar Concentration ; Potassium - physiology ; Swelling ; T lymphocytes</subject><ispartof>Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 1982-12, Vol.299 (1097), p.509-518</ispartof><rights>Copyright 1982 The Royal Society</rights><rights>Scanned images copyright © 2017, Royal Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c626t-b009f01ac215efb755c15bbd6647bab4b49be6b7e56361ae2a6632df2890019b3</citedby><cites>FETCH-LOGICAL-c626t-b009f01ac215efb755c15bbd6647bab4b49be6b7e56361ae2a6632df2890019b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2395792$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2395792$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6130543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grinstein, S.</creatorcontrib><creatorcontrib>Clarke, C. A.</creatorcontrib><creatorcontrib>Rothstein, A.</creatorcontrib><title>Increased Anion Permeability During Volume Regulation in Human Lymphocytes</title><title>Philosophical transactions of the Royal Society of London. Series B, Biological sciences</title><addtitle>Phil. Trans. R. Soc. Lond. B</addtitle><addtitle>Philos Trans R Soc Lond B Biol Sci</addtitle><description>Peripheral blood lymphocytes (p.b.ls) readjust their volumes after swelling in hypotonic media. An essential component of
the regulatory response is an increase in K$^+$ and Cl$^-$ permeability. No evidence was found
for a tightly coupled co-transport of K$^+$ and Cl$^-$. The flux of either ion proceeds normally
in the virtual absence of the transported counterion. Furthermore, alterations in membrane potential recorded during the phase
of volume readjustment can be qualitatively accounted for by an increase in Cl$^-$ conductance. In tonsillar
lymphocytes, a failure of the K$^+$-permeation path to respond to swelling leads to deficient volume recovery,
but Cl$^-$ permeability is nevertheless increased upon swelling. This further suggests that K$^+$
and Cl$^-$ are transported during volume regulation through independent pathways. Cytoplasmic free Ca$^{2+}$
appears to be involved in regulatory volume decrease. K$^+$ and Cl$^-$ fluxes similar to those
elicited by swelling can also be produced by A23187 plus Ca$^{2+}$. Moreover, swelling and shrinking can be
induced in isotonic K$^+$-rich and K$^+$-free media, respectively, by the Ca$^{2+}$
ionophore. The ion flux and volume changes produced by either swelling or internal Ca$^{2+}$ can be inhibited
by similar concentrations of quinine and phenothiazines. The inhibitory activity of the latter drugs, which are powerful antagonists
of calmodulin, suggests the participation of this Ca$^{2+}$-regulator protein in volume regulation.</description><subject>Anions</subject><subject>Anions - physiology</subject><subject>B lymphocytes</subject><subject>Calcium - physiology</subject><subject>Cations</subject><subject>Cell Membrane Permeability</subject><subject>Chloride Exchange and Co-Transport Mechanisms</subject><subject>Chlorides - physiology</subject><subject>Drug regulation</subject><subject>Erythrocytes</subject><subject>Gluconates</subject><subject>Humans</subject><subject>Ion Channels - physiology</subject><subject>Lymphocytes</subject><subject>Lymphocytes - cytology</subject><subject>Lymphocytes - physiology</subject><subject>Membrane potential</subject><subject>Osmolar Concentration</subject><subject>Potassium - physiology</subject><subject>Swelling</subject><subject>T lymphocytes</subject><issn>0962-8436</issn><issn>0080-4622</issn><issn>1471-2970</issn><issn>2054-0280</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Ustu1DAUjRCoDIUtK5CyYpfBj9iJV6iUR4tGApXC1rKTmxmPEju1E1D4epzJqNII0ZV1dR733CMnyUuM1hiJ8q0Pg15jUZI1wnn5KFnhvMAZEQV6nKyQ4CQrc8qfJs9C2COEBCvys-SMY4pYTlfJl2tbeVAB6vTCGmfTb-A7UNq0ZpjSD6M3dpv-dO3YQXoD27FVw8wyNr0aO2XTzdT1O1dNA4TnyZNGtQFeHN_z5Menj7eXV9nm6-fry4tNVnHCh0zHEA3CqiKYQaMLxirMtK45zwutdK5zoYHrAhinHCsginNK6oaUAiEsND1P3iy-vXd3I4RBdiZU0LbKghuDLBHhrGQiEtcLsfIuBA-N7L3plJ8kRnIuT87lybk8OZcXBa-PzqPuoL6nH9uKeFhw76Z4oasMDJPcu9HbOMqb77fvseDiFxHCRP9CopJilBPKuPxj-sO6mSAjQZoQRpAH2mmMf1PRh7b-95ZXi2ofBufvTyE0_gBBIowWeGe2u9_Ggzxxj0Mf7each4QMzXW-e1Ay76-cHcAOJ0LZjG0r-7qhfwEFkNM4</recordid><startdate>19821201</startdate><enddate>19821201</enddate><creator>Grinstein, S.</creator><creator>Clarke, C. A.</creator><creator>Rothstein, A.</creator><general>The Royal Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19821201</creationdate><title>Increased Anion Permeability During Volume Regulation in Human Lymphocytes</title><author>Grinstein, S. ; Clarke, C. A. ; Rothstein, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c626t-b009f01ac215efb755c15bbd6647bab4b49be6b7e56361ae2a6632df2890019b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Anions</topic><topic>Anions - physiology</topic><topic>B lymphocytes</topic><topic>Calcium - physiology</topic><topic>Cations</topic><topic>Cell Membrane Permeability</topic><topic>Chloride Exchange and Co-Transport Mechanisms</topic><topic>Chlorides - physiology</topic><topic>Drug regulation</topic><topic>Erythrocytes</topic><topic>Gluconates</topic><topic>Humans</topic><topic>Ion Channels - physiology</topic><topic>Lymphocytes</topic><topic>Lymphocytes - cytology</topic><topic>Lymphocytes - physiology</topic><topic>Membrane potential</topic><topic>Osmolar Concentration</topic><topic>Potassium - physiology</topic><topic>Swelling</topic><topic>T lymphocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grinstein, S.</creatorcontrib><creatorcontrib>Clarke, C. A.</creatorcontrib><creatorcontrib>Rothstein, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Philosophical transactions of the Royal Society of London. Series B, Biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grinstein, S.</au><au>Clarke, C. A.</au><au>Rothstein, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased Anion Permeability During Volume Regulation in Human Lymphocytes</atitle><jtitle>Philosophical transactions of the Royal Society of London. Series B, Biological sciences</jtitle><stitle>Phil. Trans. R. Soc. Lond. B</stitle><addtitle>Philos Trans R Soc Lond B Biol Sci</addtitle><date>1982-12-01</date><risdate>1982</risdate><volume>299</volume><issue>1097</issue><spage>509</spage><epage>518</epage><pages>509-518</pages><issn>0962-8436</issn><issn>0080-4622</issn><eissn>1471-2970</eissn><eissn>2054-0280</eissn><abstract>Peripheral blood lymphocytes (p.b.ls) readjust their volumes after swelling in hypotonic media. An essential component of
the regulatory response is an increase in K$^+$ and Cl$^-$ permeability. No evidence was found
for a tightly coupled co-transport of K$^+$ and Cl$^-$. The flux of either ion proceeds normally
in the virtual absence of the transported counterion. Furthermore, alterations in membrane potential recorded during the phase
of volume readjustment can be qualitatively accounted for by an increase in Cl$^-$ conductance. In tonsillar
lymphocytes, a failure of the K$^+$-permeation path to respond to swelling leads to deficient volume recovery,
but Cl$^-$ permeability is nevertheless increased upon swelling. This further suggests that K$^+$
and Cl$^-$ are transported during volume regulation through independent pathways. Cytoplasmic free Ca$^{2+}$
appears to be involved in regulatory volume decrease. K$^+$ and Cl$^-$ fluxes similar to those
elicited by swelling can also be produced by A23187 plus Ca$^{2+}$. Moreover, swelling and shrinking can be
induced in isotonic K$^+$-rich and K$^+$-free media, respectively, by the Ca$^{2+}$
ionophore. The ion flux and volume changes produced by either swelling or internal Ca$^{2+}$ can be inhibited
by similar concentrations of quinine and phenothiazines. The inhibitory activity of the latter drugs, which are powerful antagonists
of calmodulin, suggests the participation of this Ca$^{2+}$-regulator protein in volume regulation.</abstract><cop>London</cop><pub>The Royal Society</pub><pmid>6130543</pmid><doi>10.1098/rstb.1982.0148</doi><tpages>10</tpages></addata></record> |
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source | MEDLINE; JSTOR Archive Collection A-Z Listing |
subjects | Anions Anions - physiology B lymphocytes Calcium - physiology Cations Cell Membrane Permeability Chloride Exchange and Co-Transport Mechanisms Chlorides - physiology Drug regulation Erythrocytes Gluconates Humans Ion Channels - physiology Lymphocytes Lymphocytes - cytology Lymphocytes - physiology Membrane potential Osmolar Concentration Potassium - physiology Swelling T lymphocytes |
title | Increased Anion Permeability During Volume Regulation in Human Lymphocytes |
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