Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly

Polynucleotide phosphorylase (PNPase) is an exoribonuclease that cleaves single-stranded RNA substrates with 3′–5′ directionality and processive behaviour. Its ring-like, trimeric architecture creates a central channel where phosphorolytic active sites reside. One face of the ring is decorated with...

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Veröffentlicht in:	Open biology 2012-04, Vol.2 (4), p.120028-120028
Hauptverfasser:	Hardwick, Steven W., Gubbey, Tobias, Hug, Isabelle, Jenal, Urs, Luisi, Ben F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Catalytic Domain Caulobacter crescentus Caulobacter crescentus - enzymology Caulobacter crescentus - genetics Caulobacter crescentus - metabolism Conformational Asymmetry Crystallography, X-Ray Endoribonucleases - chemistry Endoribonucleases - genetics Endoribonucleases - metabolism Exoribonucleases - chemistry Exoribonucleases - genetics Exoribonucleases - metabolism Models, Molecular Molecular Ratchet Multienzyme Complexes - metabolism Mutation Polynucleotide Phosphorylase Polyribonucleotide Nucleotidyltransferase - chemistry Polyribonucleotide Nucleotidyltransferase - genetics Polyribonucleotide Nucleotidyltransferase - metabolism Protein Conformation Protein Interaction Domains and Motifs Protein Structure, Quaternary Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism RNA degradosome RNA Helicases - metabolism RNA, Bacterial - genetics RNA, Bacterial - metabolism RNA–protein interactions Substrate Specificity
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