Active Nuclear Import of Mammalian Cell-Expressible DNA Origami
DNA origami enables the creation of complex 3D shapes from genetic material. Future uses could include the delivery of genetic instructions to cells, but nuclear import remains a major barrier to gene delivery due to the impermeability of the nuclear membrane. Here we realize active nuclear import o...
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| Veröffentlicht in: | Journal of the American Chemical Society 2023-03, Vol.145 (9), p.4946-4950 |
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| container_issue | 9 |
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| container_title | Journal of the American Chemical Society |
| container_volume | 145 |
| creator | Liedl, Anna Grießing, Johanna Kretzmann, Jessica A. Dietz, Hendrik |
| description | DNA origami enables the creation of complex 3D shapes from genetic material. Future uses could include the delivery of genetic instructions to cells, but nuclear import remains a major barrier to gene delivery due to the impermeability of the nuclear membrane. Here we realize active nuclear import of DNA origami objects in dividing and chemically arrested mammalian cells. We developed a custom DNA origami single-strand scaffold featuring a mammalian-cell expressible reporter gene (mCherry) and multiple Simian virus 40 (SV40) derived DNA nuclear targeting sequences (DTS). Inclusion of the DTS within DNA origami rescued gene expression in arrested cells, indicating that active transport into the nucleus occurs. Our work successfully adapts mechanisms known from viruses to promote the cellular expression of genetic instructions encoded within DNA origami objects. |
| doi_str_mv | 10.1021/jacs.2c12733 |
| format | Article |
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Future uses could include the delivery of genetic instructions to cells, but nuclear import remains a major barrier to gene delivery due to the impermeability of the nuclear membrane. Here we realize active nuclear import of DNA origami objects in dividing and chemically arrested mammalian cells. We developed a custom DNA origami single-strand scaffold featuring a mammalian-cell expressible reporter gene (mCherry) and multiple Simian virus 40 (SV40) derived DNA nuclear targeting sequences (DTS). Inclusion of the DTS within DNA origami rescued gene expression in arrested cells, indicating that active transport into the nucleus occurs. 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| ispartof | Journal of the American Chemical Society, 2023-03, Vol.145 (9), p.4946-4950 |
| issn | 0002-7863 1520-5126 |
| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Active Transport, Cell Nucleus Animals Cell Nucleus - metabolism Communication DNA - metabolism Genes, Reporter Mammals - metabolism |
| title | Active Nuclear Import of Mammalian Cell-Expressible DNA Origami |
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