Molecular epidemiology of Streptococcus pneumoniae isosslated from children with community-acquired pneumonia under 5 years in Chengdu, China
( ) is one of the most common community-associated pathogens responsible for pneumonia in children. This retrospective study aimed to investigate the molecular characteristics of isolated from children with community-acquired pneumonia (CAP) under 5 years in Chengdu, China. Molecular characteristics...
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| Veröffentlicht in: | Epidemiology and infection 2022-12, Vol.151, p.e2-e2 |
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| description | (
) is one of the most common community-associated pathogens responsible for pneumonia in children. This retrospective study aimed to investigate the molecular characteristics of
isolated from children with community-acquired pneumonia (CAP) under 5 years in Chengdu, China. Molecular characteristics of
included serotype and virulence factor performed by using PCR method and sequence types (STs) determined by sequencing seven housekeeping genes. In addition, the potential relationships between molecular characteristics were depicted by minimum spanning tree and correspondence analysis. The prevailing serotypes were 19F (18.52%), 6B (17.59%), 19A (13.89%), 6A (6.48%) and 23F (5.56%) among 108 isolates. The overall coverage rates of 7-valent, 10-valent, 13-valent, 15-valent and 20-valent pneumococcal conjugate vaccines (PCVs) were 47.32, 48.1, 75, 75 and 78.7%, respectively. Meanwhile, the coverage rates of PCV13 among the isolates from CAP patients aged |
| doi_str_mv | 10.1017/S0950268822001881 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9990402</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2754504931</sourcerecordid><originalsourceid>FETCH-LOGICAL-p266t-de65b16eacf946643902f3de76405a1d9b7becb48a397958d7ad1d875a0a3de23</originalsourceid><addsrcrecordid>eNpVkU1P3DAQhq2qVdnS_oBekI89NMVObCe-IKFVvyQQB-AcTezJrivHDnYM2h_R_8yuShGcZqTnmfc9DCGfOfvGGW9Pr5mWrFZdV9eM8a7jb8iKC6UrIZh-S1YHXB34EfmQ8x_GmK679j05apTkkim1In8vo0dTPCSKs7M4uejjZkfjSK-XhPMSTTSmZDoHLFMMDpC6HHP2sKClY4oTNVvnbcJAH9yypSZOUwlu2VVg7opLe-v5lpZgMVFJdwgpUxfoeothY8vX_eICfCTvRvAZPz3NY3L74_vN-ld1cfXz9_r8opprpZbKopIDVwhm1EIp0WhWj43FVgkmgVs9tAOaQXTQ6FbLzrZgue1aCQz2Wt0ck7N_uXMZJrQGw5LA93NyE6RdH8H1r0lw234T73utNRPsEPDlKSDFu4J56SeXDXoPAWPJfd1KIZnQDd-rJy-7nkv-_6B5BB-QjmU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2754504931</pqid></control><display><type>article</type><title>Molecular epidemiology of Streptococcus pneumoniae isosslated from children with community-acquired pneumonia under 5 years in Chengdu, China</title><source>MEDLINE</source><source>Cambridge Journals Open Access</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><creator>Chen, Haojun ; Liu, Chenggui</creator><creatorcontrib>Chen, Haojun ; Liu, Chenggui</creatorcontrib><description>(
) is one of the most common community-associated pathogens responsible for pneumonia in children. This retrospective study aimed to investigate the molecular characteristics of
isolated from children with community-acquired pneumonia (CAP) under 5 years in Chengdu, China. Molecular characteristics of
included serotype and virulence factor performed by using PCR method and sequence types (STs) determined by sequencing seven housekeeping genes. In addition, the potential relationships between molecular characteristics were depicted by minimum spanning tree and correspondence analysis. The prevailing serotypes were 19F (18.52%), 6B (17.59%), 19A (13.89%), 6A (6.48%) and 23F (5.56%) among 108 isolates. The overall coverage rates of 7-valent, 10-valent, 13-valent, 15-valent and 20-valent pneumococcal conjugate vaccines (PCVs) were 47.32, 48.1, 75, 75 and 78.7%, respectively. Meanwhile, the coverage rates of PCV13 among the isolates from CAP patients aged <1 year were high up to 84.2%. MLST analysis results showed that there were 56 different STs identified, of which the dominant STs were ST271 (22.22%) and ST320 (12.04%). Five international clones among STs were observed, including Spain
-1, Spain
-2, Taiwan
-14, Netherlands
-31 and Denmark
-32. Additionally, most of the isolates carried
,
,
,
,
and CC271 isolates expressed more of
than non-CC271 isolates. Moreover, there were strong relevant relationships among STs, serotypes and virulence factors. Considering serotypes and virulence factors together can be used as the foundation for the formulation of vaccine strategy.</description><identifier>ISSN: 0950-2688</identifier><identifier>EISSN: 1469-4409</identifier><identifier>DOI: 10.1017/S0950268822001881</identifier><identifier>PMID: 36515066</identifier><language>eng</language><publisher>England: Cambridge University Press</publisher><subject>Child ; China - epidemiology ; Community-Acquired Infections - epidemiology ; Drug Resistance, Bacterial - genetics ; Humans ; Infant ; Molecular Epidemiology ; Multilocus Sequence Typing ; Original Paper ; Pneumococcal Infections - epidemiology ; Pneumococcal Infections - prevention & control ; Pneumococcal Vaccines ; Pneumonia ; Retrospective Studies ; Serogroup ; Serotyping ; Streptococcus pneumoniae ; Virulence Factors - genetics</subject><ispartof>Epidemiology and infection, 2022-12, Vol.151, p.e2-e2</ispartof><rights>The Author(s) 2022 2022 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-1642-3019</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9990402/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9990402/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36515066$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Haojun</creatorcontrib><creatorcontrib>Liu, Chenggui</creatorcontrib><title>Molecular epidemiology of Streptococcus pneumoniae isosslated from children with community-acquired pneumonia under 5 years in Chengdu, China</title><title>Epidemiology and infection</title><addtitle>Epidemiol Infect</addtitle><description>(
) is one of the most common community-associated pathogens responsible for pneumonia in children. This retrospective study aimed to investigate the molecular characteristics of
isolated from children with community-acquired pneumonia (CAP) under 5 years in Chengdu, China. Molecular characteristics of
included serotype and virulence factor performed by using PCR method and sequence types (STs) determined by sequencing seven housekeeping genes. In addition, the potential relationships between molecular characteristics were depicted by minimum spanning tree and correspondence analysis. The prevailing serotypes were 19F (18.52%), 6B (17.59%), 19A (13.89%), 6A (6.48%) and 23F (5.56%) among 108 isolates. The overall coverage rates of 7-valent, 10-valent, 13-valent, 15-valent and 20-valent pneumococcal conjugate vaccines (PCVs) were 47.32, 48.1, 75, 75 and 78.7%, respectively. Meanwhile, the coverage rates of PCV13 among the isolates from CAP patients aged <1 year were high up to 84.2%. MLST analysis results showed that there were 56 different STs identified, of which the dominant STs were ST271 (22.22%) and ST320 (12.04%). Five international clones among STs were observed, including Spain
-1, Spain
-2, Taiwan
-14, Netherlands
-31 and Denmark
-32. Additionally, most of the isolates carried
,
,
,
,
and CC271 isolates expressed more of
than non-CC271 isolates. Moreover, there were strong relevant relationships among STs, serotypes and virulence factors. Considering serotypes and virulence factors together can be used as the foundation for the formulation of vaccine strategy.</description><subject>Child</subject><subject>China - epidemiology</subject><subject>Community-Acquired Infections - epidemiology</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Humans</subject><subject>Infant</subject><subject>Molecular Epidemiology</subject><subject>Multilocus Sequence Typing</subject><subject>Original Paper</subject><subject>Pneumococcal Infections - epidemiology</subject><subject>Pneumococcal Infections - prevention & control</subject><subject>Pneumococcal Vaccines</subject><subject>Pneumonia</subject><subject>Retrospective Studies</subject><subject>Serogroup</subject><subject>Serotyping</subject><subject>Streptococcus pneumoniae</subject><subject>Virulence Factors - genetics</subject><issn>0950-2688</issn><issn>1469-4409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1P3DAQhq2qVdnS_oBekI89NMVObCe-IKFVvyQQB-AcTezJrivHDnYM2h_R_8yuShGcZqTnmfc9DCGfOfvGGW9Pr5mWrFZdV9eM8a7jb8iKC6UrIZh-S1YHXB34EfmQ8x_GmK679j05apTkkim1In8vo0dTPCSKs7M4uejjZkfjSK-XhPMSTTSmZDoHLFMMDpC6HHP2sKClY4oTNVvnbcJAH9yypSZOUwlu2VVg7opLe-v5lpZgMVFJdwgpUxfoeothY8vX_eICfCTvRvAZPz3NY3L74_vN-ld1cfXz9_r8opprpZbKopIDVwhm1EIp0WhWj43FVgkmgVs9tAOaQXTQ6FbLzrZgue1aCQz2Wt0ck7N_uXMZJrQGw5LA93NyE6RdH8H1r0lw234T73utNRPsEPDlKSDFu4J56SeXDXoPAWPJfd1KIZnQDd-rJy-7nkv-_6B5BB-QjmU</recordid><startdate>20221214</startdate><enddate>20221214</enddate><creator>Chen, Haojun</creator><creator>Liu, Chenggui</creator><general>Cambridge University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1642-3019</orcidid></search><sort><creationdate>20221214</creationdate><title>Molecular epidemiology of Streptococcus pneumoniae isosslated from children with community-acquired pneumonia under 5 years in Chengdu, China</title><author>Chen, Haojun ; Liu, Chenggui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-de65b16eacf946643902f3de76405a1d9b7becb48a397958d7ad1d875a0a3de23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Child</topic><topic>China - epidemiology</topic><topic>Community-Acquired Infections - epidemiology</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Humans</topic><topic>Infant</topic><topic>Molecular Epidemiology</topic><topic>Multilocus Sequence Typing</topic><topic>Original Paper</topic><topic>Pneumococcal Infections - epidemiology</topic><topic>Pneumococcal Infections - prevention & control</topic><topic>Pneumococcal Vaccines</topic><topic>Pneumonia</topic><topic>Retrospective Studies</topic><topic>Serogroup</topic><topic>Serotyping</topic><topic>Streptococcus pneumoniae</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Haojun</creatorcontrib><creatorcontrib>Liu, Chenggui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Epidemiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Haojun</au><au>Liu, Chenggui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular epidemiology of Streptococcus pneumoniae isosslated from children with community-acquired pneumonia under 5 years in Chengdu, China</atitle><jtitle>Epidemiology and infection</jtitle><addtitle>Epidemiol Infect</addtitle><date>2022-12-14</date><risdate>2022</risdate><volume>151</volume><spage>e2</spage><epage>e2</epage><pages>e2-e2</pages><issn>0950-2688</issn><eissn>1469-4409</eissn><abstract>(
) is one of the most common community-associated pathogens responsible for pneumonia in children. This retrospective study aimed to investigate the molecular characteristics of
isolated from children with community-acquired pneumonia (CAP) under 5 years in Chengdu, China. Molecular characteristics of
included serotype and virulence factor performed by using PCR method and sequence types (STs) determined by sequencing seven housekeeping genes. In addition, the potential relationships between molecular characteristics were depicted by minimum spanning tree and correspondence analysis. The prevailing serotypes were 19F (18.52%), 6B (17.59%), 19A (13.89%), 6A (6.48%) and 23F (5.56%) among 108 isolates. The overall coverage rates of 7-valent, 10-valent, 13-valent, 15-valent and 20-valent pneumococcal conjugate vaccines (PCVs) were 47.32, 48.1, 75, 75 and 78.7%, respectively. Meanwhile, the coverage rates of PCV13 among the isolates from CAP patients aged <1 year were high up to 84.2%. MLST analysis results showed that there were 56 different STs identified, of which the dominant STs were ST271 (22.22%) and ST320 (12.04%). Five international clones among STs were observed, including Spain
-1, Spain
-2, Taiwan
-14, Netherlands
-31 and Denmark
-32. Additionally, most of the isolates carried
,
,
,
,
and CC271 isolates expressed more of
than non-CC271 isolates. Moreover, there were strong relevant relationships among STs, serotypes and virulence factors. Considering serotypes and virulence factors together can be used as the foundation for the formulation of vaccine strategy.</abstract><cop>England</cop><pub>Cambridge University Press</pub><pmid>36515066</pmid><doi>10.1017/S0950268822001881</doi><orcidid>https://orcid.org/0000-0003-1642-3019</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Cambridge Journals Open Access; DOAJ Directory of Open Access Journals; PubMed Central |
| subjects | Child China - epidemiology Community-Acquired Infections - epidemiology Drug Resistance, Bacterial - genetics Humans Infant Molecular Epidemiology Multilocus Sequence Typing Original Paper Pneumococcal Infections - epidemiology Pneumococcal Infections - prevention & control Pneumococcal Vaccines Pneumonia Retrospective Studies Serogroup Serotyping Streptococcus pneumoniae Virulence Factors - genetics |
| title | Molecular epidemiology of Streptococcus pneumoniae isosslated from children with community-acquired pneumonia under 5 years in Chengdu, China |
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