Protective Effects of Crocin Against Methotrexate-Induced Hepatotoxicity in Adult Male Albino Rats: Histological, Immunohistochemical, and Biochemical Study
Among the many known adverse effects of methotrexate (MTX), hepatotoxicity stands out as a major drawback that limits its therapeutic applicability. There is growing evidence that crocin has antioxidant, anti-hyperglycemic, cardioprotective, and anti-inflammatory effects. This study's aim is to...
Gespeichert in:
Veröffentlicht in: | Curēus (Palo Alto, CA) CA), 2023-01, Vol.15 (1), p.e34468-e34468 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | e34468 |
---|---|
container_issue | 1 |
container_start_page | e34468 |
container_title | Curēus (Palo Alto, CA) |
container_volume | 15 |
creator | AbdelKader, Ghada Abdelaziz, Eman Z Hassan, Ranya Greish, Sahar M Abogresha, Noha M Sultan, Basma O Yousef, Einas M Morsi, Shereen |
description | Among the many known adverse effects of methotrexate (MTX), hepatotoxicity stands out as a major drawback that limits its therapeutic applicability. There is growing evidence that crocin has antioxidant, anti-hyperglycemic, cardioprotective, and anti-inflammatory effects. This study's aim is to evaluate the potential protective effect of crocin against MTX-induced liver damage in rats using biochemical, histological, and immunohistochemical analyses.
Twenty-four adult male albino rats were split into four groups at random (six rats/group) as follows: normal control (saline, intraperitoneal (i.p.) injections), crocin-treated (100 mg/kg daily for 14 days, i.p.), MTX-treated (20 mg/kg single i.p. injection on day 15), and crocin/MTX-treated groups (crocin 100 mg/kg/day for 14 days, i.p. + MTX 20 mg/kg single i.p. injection on day 15). On day 16 of the experiment, blood and tissue specimens were used to assess the liver functions, oxidative stress markers, transforming growth factor beta 1 (
), caspase-3,
-associated X protein (
), and B-cell lymphoma 2 (
) expression.
The results of the current research revealed the protective actions of crocin against MTX-induced hepatotoxicity. Our results showed that crocin possesses antioxidants (decrease malondialdehyde (MDA), increase glutathione (GSH) levels, and enhance catalase (CAT) and superoxide dismutase (SOD) enzymatic activity), anti-fibrotic (decrease
), and anti-apoptotic (decrease
and
expression while increase
) actions in liver. Moreover, crocin administration along with MTX restores the normal histological structure of hepatic tissues.
The data presented in the current study using an in vivo animal model support the notion that crocin should be further studied in humans to assess its potential hepatoprotective effects against MTX-induced liver damage. |
doi_str_mv | 10.7759/cureus.34468 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9981239</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2783791100</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2578-94e702371f138db7dd174b9bdb71665a2454ea6b18fbbc3d72b427e3ea628533</originalsourceid><addsrcrecordid>eNpdkUtvEzEUhUcIRKvSHWtkiQ2LTvFjMrZZIKVRIZGKQNC95bHvJK5m7OBH1fwXfiwTUqLC6h6d--noXp2qek3wJecz-d6UCCVdsqZpxbPqlJJW1IKI5vkTfVKdp3SHMSaYU8zxy-qEtYI3LSen1a9vMWQw2d0Duu77SSUUerSIwTiP5mvtfMroC-RNyBEedIZ65W0xYNEStjqHHB6ccXmH9rgtwwTrAdB86JwP6LvO6QNaupTDENbO6OECrcax-LDZe2YD48HU3qIrdzTQj1zs7lX1otdDgvPHeVbdfrq-XSzrm6-fV4v5TW3ojItaNsAxZZz0hAnbcWsJbzrZTZK07UzTZtaAbjsi-q4zzHLaNZQDmzwqZoydVR8PsdvSjWAN-Bz1oLbRjTruVNBO_bvxbqPW4V5JKQhlcgp49xgQw88CKavRJQPDoD2EkhTlgnFJCMYT-vY_9C6U6KfvJkoyhqWQ-4suDpSJIaUI_fEYgtW-eHUoXv0pfsLfPH3gCP-tmf0GoKatjA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2793309893</pqid></control><display><type>article</type><title>Protective Effects of Crocin Against Methotrexate-Induced Hepatotoxicity in Adult Male Albino Rats: Histological, Immunohistochemical, and Biochemical Study</title><source>PubMed Central Open Access</source><source>PubMed Central</source><creator>AbdelKader, Ghada ; Abdelaziz, Eman Z ; Hassan, Ranya ; Greish, Sahar M ; Abogresha, Noha M ; Sultan, Basma O ; Yousef, Einas M ; Morsi, Shereen</creator><creatorcontrib>AbdelKader, Ghada ; Abdelaziz, Eman Z ; Hassan, Ranya ; Greish, Sahar M ; Abogresha, Noha M ; Sultan, Basma O ; Yousef, Einas M ; Morsi, Shereen</creatorcontrib><description>Among the many known adverse effects of methotrexate (MTX), hepatotoxicity stands out as a major drawback that limits its therapeutic applicability. There is growing evidence that crocin has antioxidant, anti-hyperglycemic, cardioprotective, and anti-inflammatory effects. This study's aim is to evaluate the potential protective effect of crocin against MTX-induced liver damage in rats using biochemical, histological, and immunohistochemical analyses.
Twenty-four adult male albino rats were split into four groups at random (six rats/group) as follows: normal control (saline, intraperitoneal (i.p.) injections), crocin-treated (100 mg/kg daily for 14 days, i.p.), MTX-treated (20 mg/kg single i.p. injection on day 15), and crocin/MTX-treated groups (crocin 100 mg/kg/day for 14 days, i.p. + MTX 20 mg/kg single i.p. injection on day 15). On day 16 of the experiment, blood and tissue specimens were used to assess the liver functions, oxidative stress markers, transforming growth factor beta 1 (
), caspase-3,
-associated X protein (
), and B-cell lymphoma 2 (
) expression.
The results of the current research revealed the protective actions of crocin against MTX-induced hepatotoxicity. Our results showed that crocin possesses antioxidants (decrease malondialdehyde (MDA), increase glutathione (GSH) levels, and enhance catalase (CAT) and superoxide dismutase (SOD) enzymatic activity), anti-fibrotic (decrease
), and anti-apoptotic (decrease
and
expression while increase
) actions in liver. Moreover, crocin administration along with MTX restores the normal histological structure of hepatic tissues.
The data presented in the current study using an in vivo animal model support the notion that crocin should be further studied in humans to assess its potential hepatoprotective effects against MTX-induced liver damage.</description><identifier>ISSN: 2168-8184</identifier><identifier>EISSN: 2168-8184</identifier><identifier>DOI: 10.7759/cureus.34468</identifier><identifier>PMID: 36874671</identifier><language>eng</language><publisher>United States: Cureus Inc</publisher><subject>Anatomy ; Antioxidants ; Apoptosis ; Cytotoxicity ; Dehydrogenases ; Deoxyribonucleic acid ; DNA ; Enzymes ; Experiments ; Homocysteine ; Liver cirrhosis ; Oxidative stress ; Pathology ; Proteins</subject><ispartof>Curēus (Palo Alto, CA), 2023-01, Vol.15 (1), p.e34468-e34468</ispartof><rights>Copyright © 2023, AbdelKader et al.</rights><rights>Copyright © 2023, AbdelKader et al. This work is published under https://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2023, AbdelKader et al. 2023 AbdelKader et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2578-94e702371f138db7dd174b9bdb71665a2454ea6b18fbbc3d72b427e3ea628533</citedby><cites>FETCH-LOGICAL-c2578-94e702371f138db7dd174b9bdb71665a2454ea6b18fbbc3d72b427e3ea628533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981239/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981239/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36874671$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>AbdelKader, Ghada</creatorcontrib><creatorcontrib>Abdelaziz, Eman Z</creatorcontrib><creatorcontrib>Hassan, Ranya</creatorcontrib><creatorcontrib>Greish, Sahar M</creatorcontrib><creatorcontrib>Abogresha, Noha M</creatorcontrib><creatorcontrib>Sultan, Basma O</creatorcontrib><creatorcontrib>Yousef, Einas M</creatorcontrib><creatorcontrib>Morsi, Shereen</creatorcontrib><title>Protective Effects of Crocin Against Methotrexate-Induced Hepatotoxicity in Adult Male Albino Rats: Histological, Immunohistochemical, and Biochemical Study</title><title>Curēus (Palo Alto, CA)</title><addtitle>Cureus</addtitle><description>Among the many known adverse effects of methotrexate (MTX), hepatotoxicity stands out as a major drawback that limits its therapeutic applicability. There is growing evidence that crocin has antioxidant, anti-hyperglycemic, cardioprotective, and anti-inflammatory effects. This study's aim is to evaluate the potential protective effect of crocin against MTX-induced liver damage in rats using biochemical, histological, and immunohistochemical analyses.
Twenty-four adult male albino rats were split into four groups at random (six rats/group) as follows: normal control (saline, intraperitoneal (i.p.) injections), crocin-treated (100 mg/kg daily for 14 days, i.p.), MTX-treated (20 mg/kg single i.p. injection on day 15), and crocin/MTX-treated groups (crocin 100 mg/kg/day for 14 days, i.p. + MTX 20 mg/kg single i.p. injection on day 15). On day 16 of the experiment, blood and tissue specimens were used to assess the liver functions, oxidative stress markers, transforming growth factor beta 1 (
), caspase-3,
-associated X protein (
), and B-cell lymphoma 2 (
) expression.
The results of the current research revealed the protective actions of crocin against MTX-induced hepatotoxicity. Our results showed that crocin possesses antioxidants (decrease malondialdehyde (MDA), increase glutathione (GSH) levels, and enhance catalase (CAT) and superoxide dismutase (SOD) enzymatic activity), anti-fibrotic (decrease
), and anti-apoptotic (decrease
and
expression while increase
) actions in liver. Moreover, crocin administration along with MTX restores the normal histological structure of hepatic tissues.
The data presented in the current study using an in vivo animal model support the notion that crocin should be further studied in humans to assess its potential hepatoprotective effects against MTX-induced liver damage.</description><subject>Anatomy</subject><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Cytotoxicity</subject><subject>Dehydrogenases</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Enzymes</subject><subject>Experiments</subject><subject>Homocysteine</subject><subject>Liver cirrhosis</subject><subject>Oxidative stress</subject><subject>Pathology</subject><subject>Proteins</subject><issn>2168-8184</issn><issn>2168-8184</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkUtvEzEUhUcIRKvSHWtkiQ2LTvFjMrZZIKVRIZGKQNC95bHvJK5m7OBH1fwXfiwTUqLC6h6d--noXp2qek3wJecz-d6UCCVdsqZpxbPqlJJW1IKI5vkTfVKdp3SHMSaYU8zxy-qEtYI3LSen1a9vMWQw2d0Duu77SSUUerSIwTiP5mvtfMroC-RNyBEedIZ65W0xYNEStjqHHB6ccXmH9rgtwwTrAdB86JwP6LvO6QNaupTDENbO6OECrcax-LDZe2YD48HU3qIrdzTQj1zs7lX1otdDgvPHeVbdfrq-XSzrm6-fV4v5TW3ojItaNsAxZZz0hAnbcWsJbzrZTZK07UzTZtaAbjsi-q4zzHLaNZQDmzwqZoydVR8PsdvSjWAN-Bz1oLbRjTruVNBO_bvxbqPW4V5JKQhlcgp49xgQw88CKavRJQPDoD2EkhTlgnFJCMYT-vY_9C6U6KfvJkoyhqWQ-4suDpSJIaUI_fEYgtW-eHUoXv0pfsLfPH3gCP-tmf0GoKatjA</recordid><startdate>202301</startdate><enddate>202301</enddate><creator>AbdelKader, Ghada</creator><creator>Abdelaziz, Eman Z</creator><creator>Hassan, Ranya</creator><creator>Greish, Sahar M</creator><creator>Abogresha, Noha M</creator><creator>Sultan, Basma O</creator><creator>Yousef, Einas M</creator><creator>Morsi, Shereen</creator><general>Cureus Inc</general><general>Cureus</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>202301</creationdate><title>Protective Effects of Crocin Against Methotrexate-Induced Hepatotoxicity in Adult Male Albino Rats: Histological, Immunohistochemical, and Biochemical Study</title><author>AbdelKader, Ghada ; Abdelaziz, Eman Z ; Hassan, Ranya ; Greish, Sahar M ; Abogresha, Noha M ; Sultan, Basma O ; Yousef, Einas M ; Morsi, Shereen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2578-94e702371f138db7dd174b9bdb71665a2454ea6b18fbbc3d72b427e3ea628533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Anatomy</topic><topic>Antioxidants</topic><topic>Apoptosis</topic><topic>Cytotoxicity</topic><topic>Dehydrogenases</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Enzymes</topic><topic>Experiments</topic><topic>Homocysteine</topic><topic>Liver cirrhosis</topic><topic>Oxidative stress</topic><topic>Pathology</topic><topic>Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>AbdelKader, Ghada</creatorcontrib><creatorcontrib>Abdelaziz, Eman Z</creatorcontrib><creatorcontrib>Hassan, Ranya</creatorcontrib><creatorcontrib>Greish, Sahar M</creatorcontrib><creatorcontrib>Abogresha, Noha M</creatorcontrib><creatorcontrib>Sultan, Basma O</creatorcontrib><creatorcontrib>Yousef, Einas M</creatorcontrib><creatorcontrib>Morsi, Shereen</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Curēus (Palo Alto, CA)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>AbdelKader, Ghada</au><au>Abdelaziz, Eman Z</au><au>Hassan, Ranya</au><au>Greish, Sahar M</au><au>Abogresha, Noha M</au><au>Sultan, Basma O</au><au>Yousef, Einas M</au><au>Morsi, Shereen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective Effects of Crocin Against Methotrexate-Induced Hepatotoxicity in Adult Male Albino Rats: Histological, Immunohistochemical, and Biochemical Study</atitle><jtitle>Curēus (Palo Alto, CA)</jtitle><addtitle>Cureus</addtitle><date>2023-01</date><risdate>2023</risdate><volume>15</volume><issue>1</issue><spage>e34468</spage><epage>e34468</epage><pages>e34468-e34468</pages><issn>2168-8184</issn><eissn>2168-8184</eissn><abstract>Among the many known adverse effects of methotrexate (MTX), hepatotoxicity stands out as a major drawback that limits its therapeutic applicability. There is growing evidence that crocin has antioxidant, anti-hyperglycemic, cardioprotective, and anti-inflammatory effects. This study's aim is to evaluate the potential protective effect of crocin against MTX-induced liver damage in rats using biochemical, histological, and immunohistochemical analyses.
Twenty-four adult male albino rats were split into four groups at random (six rats/group) as follows: normal control (saline, intraperitoneal (i.p.) injections), crocin-treated (100 mg/kg daily for 14 days, i.p.), MTX-treated (20 mg/kg single i.p. injection on day 15), and crocin/MTX-treated groups (crocin 100 mg/kg/day for 14 days, i.p. + MTX 20 mg/kg single i.p. injection on day 15). On day 16 of the experiment, blood and tissue specimens were used to assess the liver functions, oxidative stress markers, transforming growth factor beta 1 (
), caspase-3,
-associated X protein (
), and B-cell lymphoma 2 (
) expression.
The results of the current research revealed the protective actions of crocin against MTX-induced hepatotoxicity. Our results showed that crocin possesses antioxidants (decrease malondialdehyde (MDA), increase glutathione (GSH) levels, and enhance catalase (CAT) and superoxide dismutase (SOD) enzymatic activity), anti-fibrotic (decrease
), and anti-apoptotic (decrease
and
expression while increase
) actions in liver. Moreover, crocin administration along with MTX restores the normal histological structure of hepatic tissues.
The data presented in the current study using an in vivo animal model support the notion that crocin should be further studied in humans to assess its potential hepatoprotective effects against MTX-induced liver damage.</abstract><cop>United States</cop><pub>Cureus Inc</pub><pmid>36874671</pmid><doi>10.7759/cureus.34468</doi><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2168-8184 |
ispartof | Curēus (Palo Alto, CA), 2023-01, Vol.15 (1), p.e34468-e34468 |
issn | 2168-8184 2168-8184 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9981239 |
source | PubMed Central Open Access; PubMed Central |
subjects | Anatomy Antioxidants Apoptosis Cytotoxicity Dehydrogenases Deoxyribonucleic acid DNA Enzymes Experiments Homocysteine Liver cirrhosis Oxidative stress Pathology Proteins |
title | Protective Effects of Crocin Against Methotrexate-Induced Hepatotoxicity in Adult Male Albino Rats: Histological, Immunohistochemical, and Biochemical Study |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T11%3A32%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protective%20Effects%20of%20Crocin%20Against%20Methotrexate-Induced%20Hepatotoxicity%20in%20Adult%20Male%20Albino%20Rats:%20Histological,%20Immunohistochemical,%20and%20Biochemical%20Study&rft.jtitle=Cur%C4%93us%20(Palo%20Alto,%20CA)&rft.au=AbdelKader,%20Ghada&rft.date=2023-01&rft.volume=15&rft.issue=1&rft.spage=e34468&rft.epage=e34468&rft.pages=e34468-e34468&rft.issn=2168-8184&rft.eissn=2168-8184&rft_id=info:doi/10.7759/cureus.34468&rft_dat=%3Cproquest_pubme%3E2783791100%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2793309893&rft_id=info:pmid/36874671&rfr_iscdi=true |