Comparison of Sampling Procedures for the Molecular Diagnosis of Leishmaniases

The present work evaluates sampling protocols, storage procedures, and DNA purification methods for Leishmania spp. detection and quantification in different biological samples. The efficiency of three preservation solutions, a phosphate buffer solution, an ethylenediaminetetraacetic acid (EDTA) buf...

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Veröffentlicht in:	The American journal of tropical medicine and hygiene 2023-03, Vol.108 (3), p.548-554
Hauptverfasser:	Andrade Zampieri, Ricardo, Ide Aoki, Juliana, Müller, Karl Erik, Jon Shaw, Jeffrey, Maria Floeter-Winter, Lucile
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Comparative studies DNA, Protozoan - genetics Edetic Acid Leishmania braziliensis - genetics Leishmania infantum - genetics Leishmaniasis Mammals Medical diagnosis Mice Mice, Inbred BALB C Molecular biology Parasites Parasitic diseases Real-Time Polymerase Chain Reaction - methods Sampling techniques
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