DNA Origami Fiducial for Accurate 3D Atomic Force Microscopy Imaging
Atomic force microscopy (AFM) is a powerful technique for imaging molecules, macromolecular complexes, and nanoparticles with nanometer resolution. However, AFM images are distorted by the shape of the tip used. These distortions can be corrected if the tip shape can be determined by scanning a samp...
Gespeichert in:
| Veröffentlicht in: | Nano letters 2023-02, Vol.23 (4), p.1236-1243 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1243 |
|---|---|
| container_issue | 4 |
| container_start_page | 1236 |
| container_title | Nano letters |
| container_volume | 23 |
| creator | Kolbeck, Pauline J. Dass, Mihir Martynenko, Irina V. van Dijk-Moes, Relinde J. A. Brouwer, Kelly J. H. van Blaaderen, Alfons Vanderlinden, Willem Liedl, Tim Lipfert, Jan |
| description | Atomic force microscopy (AFM) is a powerful technique for imaging molecules, macromolecular complexes, and nanoparticles with nanometer resolution. However, AFM images are distorted by the shape of the tip used. These distortions can be corrected if the tip shape can be determined by scanning a sample with features sharper than the tip and higher than the object of interest. Here we present a 3D DNA origami structure as fiducial for tip reconstruction and image correction. Our fiducial is stable under a broad range of conditions and has sharp steps at different heights that enable reliable tip reconstruction from as few as ten fiducials. The DNA origami is readily codeposited with biological and nonbiological samples, achieves higher precision for the tip apex than polycrystalline samples, and dramatically improves the accuracy of the lateral dimensions determined from the images. Our fiducial thus enables accurate and precise AFM imaging for a broad range of applications. |
| doi_str_mv | 10.1021/acs.nanolett.2c04299 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9951250</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2773714117</sourcerecordid><originalsourceid>FETCH-LOGICAL-a449t-c73d81a4c89dd4a8839be63448271ff6ac3f8758731063dc4a35ff81b6bbda33</originalsourceid><addsrcrecordid>eNp9kDFPwzAQhS0EoqXwDxDyyNJix05sL0hVS6ES0IXdujhOMUriYidI_fekaqlgYbqT7r13dx9C15RMKEnoHZg4aaDxlW3bSWIIT5Q6QUOaMjLOlEpOj73kA3QR4wchRLGUnKMBywRPU8GGaD5_neJVcGuoHV64ojMOKlz6gKfGdAFai9kcT1tfO4MXPhiLX5wJPhq_2eJlDWvXrC_RWQlVtFeHOkJvi4e32dP4efW4nE2fx8C5asdGsEJS4EaqouAgJVO5zRjnMhG0LDMwrJQilYJRkrHCcGBpWUqaZ3leAGMjdL-P3XR5bQtjmzZApTfB1RC22oPTfyeNe9dr_6WVSmmSkj7g9hAQ_GdnY6trF42tKmis76JOhGCCckpFL-V76e7XGGx5XEOJ3vHXPX_9w18f-Pe2m98nHk0_wHsB2Qt29g_fhaYH9n_mN3VwlWc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2773714117</pqid></control><display><type>article</type><title>DNA Origami Fiducial for Accurate 3D Atomic Force Microscopy Imaging</title><source>MEDLINE</source><source>ACS Publications</source><creator>Kolbeck, Pauline J. ; Dass, Mihir ; Martynenko, Irina V. ; van Dijk-Moes, Relinde J. A. ; Brouwer, Kelly J. H. ; van Blaaderen, Alfons ; Vanderlinden, Willem ; Liedl, Tim ; Lipfert, Jan</creator><creatorcontrib>Kolbeck, Pauline J. ; Dass, Mihir ; Martynenko, Irina V. ; van Dijk-Moes, Relinde J. A. ; Brouwer, Kelly J. H. ; van Blaaderen, Alfons ; Vanderlinden, Willem ; Liedl, Tim ; Lipfert, Jan</creatorcontrib><description>Atomic force microscopy (AFM) is a powerful technique for imaging molecules, macromolecular complexes, and nanoparticles with nanometer resolution. However, AFM images are distorted by the shape of the tip used. These distortions can be corrected if the tip shape can be determined by scanning a sample with features sharper than the tip and higher than the object of interest. Here we present a 3D DNA origami structure as fiducial for tip reconstruction and image correction. Our fiducial is stable under a broad range of conditions and has sharp steps at different heights that enable reliable tip reconstruction from as few as ten fiducials. The DNA origami is readily codeposited with biological and nonbiological samples, achieves higher precision for the tip apex than polycrystalline samples, and dramatically improves the accuracy of the lateral dimensions determined from the images. Our fiducial thus enables accurate and precise AFM imaging for a broad range of applications.</description><identifier>ISSN: 1530-6984</identifier><identifier>EISSN: 1530-6992</identifier><identifier>DOI: 10.1021/acs.nanolett.2c04299</identifier><identifier>PMID: 36745573</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>DNA - chemistry ; Letter ; Microscopy, Atomic Force - methods ; Nanoparticles</subject><ispartof>Nano letters, 2023-02, Vol.23 (4), p.1236-1243</ispartof><rights>2023 The Authors. Published by American Chemical Society</rights><rights>2023 The Authors. Published by American Chemical Society 2023 The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a449t-c73d81a4c89dd4a8839be63448271ff6ac3f8758731063dc4a35ff81b6bbda33</citedby><cites>FETCH-LOGICAL-a449t-c73d81a4c89dd4a8839be63448271ff6ac3f8758731063dc4a35ff81b6bbda33</cites><orcidid>0000-0001-5239-3043 ; 0000-0002-0040-0173 ; 0000-0001-9422-8062 ; 0000-0003-3613-7896</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.nanolett.2c04299$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.nanolett.2c04299$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,780,784,885,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36745573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kolbeck, Pauline J.</creatorcontrib><creatorcontrib>Dass, Mihir</creatorcontrib><creatorcontrib>Martynenko, Irina V.</creatorcontrib><creatorcontrib>van Dijk-Moes, Relinde J. A.</creatorcontrib><creatorcontrib>Brouwer, Kelly J. H.</creatorcontrib><creatorcontrib>van Blaaderen, Alfons</creatorcontrib><creatorcontrib>Vanderlinden, Willem</creatorcontrib><creatorcontrib>Liedl, Tim</creatorcontrib><creatorcontrib>Lipfert, Jan</creatorcontrib><title>DNA Origami Fiducial for Accurate 3D Atomic Force Microscopy Imaging</title><title>Nano letters</title><addtitle>Nano Lett</addtitle><description>Atomic force microscopy (AFM) is a powerful technique for imaging molecules, macromolecular complexes, and nanoparticles with nanometer resolution. However, AFM images are distorted by the shape of the tip used. These distortions can be corrected if the tip shape can be determined by scanning a sample with features sharper than the tip and higher than the object of interest. Here we present a 3D DNA origami structure as fiducial for tip reconstruction and image correction. Our fiducial is stable under a broad range of conditions and has sharp steps at different heights that enable reliable tip reconstruction from as few as ten fiducials. The DNA origami is readily codeposited with biological and nonbiological samples, achieves higher precision for the tip apex than polycrystalline samples, and dramatically improves the accuracy of the lateral dimensions determined from the images. Our fiducial thus enables accurate and precise AFM imaging for a broad range of applications.</description><subject>DNA - chemistry</subject><subject>Letter</subject><subject>Microscopy, Atomic Force - methods</subject><subject>Nanoparticles</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDFPwzAQhS0EoqXwDxDyyNJix05sL0hVS6ES0IXdujhOMUriYidI_fekaqlgYbqT7r13dx9C15RMKEnoHZg4aaDxlW3bSWIIT5Q6QUOaMjLOlEpOj73kA3QR4wchRLGUnKMBywRPU8GGaD5_neJVcGuoHV64ojMOKlz6gKfGdAFai9kcT1tfO4MXPhiLX5wJPhq_2eJlDWvXrC_RWQlVtFeHOkJvi4e32dP4efW4nE2fx8C5asdGsEJS4EaqouAgJVO5zRjnMhG0LDMwrJQilYJRkrHCcGBpWUqaZ3leAGMjdL-P3XR5bQtjmzZApTfB1RC22oPTfyeNe9dr_6WVSmmSkj7g9hAQ_GdnY6trF42tKmis76JOhGCCckpFL-V76e7XGGx5XEOJ3vHXPX_9w18f-Pe2m98nHk0_wHsB2Qt29g_fhaYH9n_mN3VwlWc</recordid><startdate>20230222</startdate><enddate>20230222</enddate><creator>Kolbeck, Pauline J.</creator><creator>Dass, Mihir</creator><creator>Martynenko, Irina V.</creator><creator>van Dijk-Moes, Relinde J. A.</creator><creator>Brouwer, Kelly J. H.</creator><creator>van Blaaderen, Alfons</creator><creator>Vanderlinden, Willem</creator><creator>Liedl, Tim</creator><creator>Lipfert, Jan</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5239-3043</orcidid><orcidid>https://orcid.org/0000-0002-0040-0173</orcidid><orcidid>https://orcid.org/0000-0001-9422-8062</orcidid><orcidid>https://orcid.org/0000-0003-3613-7896</orcidid></search><sort><creationdate>20230222</creationdate><title>DNA Origami Fiducial for Accurate 3D Atomic Force Microscopy Imaging</title><author>Kolbeck, Pauline J. ; Dass, Mihir ; Martynenko, Irina V. ; van Dijk-Moes, Relinde J. A. ; Brouwer, Kelly J. H. ; van Blaaderen, Alfons ; Vanderlinden, Willem ; Liedl, Tim ; Lipfert, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a449t-c73d81a4c89dd4a8839be63448271ff6ac3f8758731063dc4a35ff81b6bbda33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>DNA - chemistry</topic><topic>Letter</topic><topic>Microscopy, Atomic Force - methods</topic><topic>Nanoparticles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kolbeck, Pauline J.</creatorcontrib><creatorcontrib>Dass, Mihir</creatorcontrib><creatorcontrib>Martynenko, Irina V.</creatorcontrib><creatorcontrib>van Dijk-Moes, Relinde J. A.</creatorcontrib><creatorcontrib>Brouwer, Kelly J. H.</creatorcontrib><creatorcontrib>van Blaaderen, Alfons</creatorcontrib><creatorcontrib>Vanderlinden, Willem</creatorcontrib><creatorcontrib>Liedl, Tim</creatorcontrib><creatorcontrib>Lipfert, Jan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nano letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kolbeck, Pauline J.</au><au>Dass, Mihir</au><au>Martynenko, Irina V.</au><au>van Dijk-Moes, Relinde J. A.</au><au>Brouwer, Kelly J. H.</au><au>van Blaaderen, Alfons</au><au>Vanderlinden, Willem</au><au>Liedl, Tim</au><au>Lipfert, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Origami Fiducial for Accurate 3D Atomic Force Microscopy Imaging</atitle><jtitle>Nano letters</jtitle><addtitle>Nano Lett</addtitle><date>2023-02-22</date><risdate>2023</risdate><volume>23</volume><issue>4</issue><spage>1236</spage><epage>1243</epage><pages>1236-1243</pages><issn>1530-6984</issn><eissn>1530-6992</eissn><abstract>Atomic force microscopy (AFM) is a powerful technique for imaging molecules, macromolecular complexes, and nanoparticles with nanometer resolution. However, AFM images are distorted by the shape of the tip used. These distortions can be corrected if the tip shape can be determined by scanning a sample with features sharper than the tip and higher than the object of interest. Here we present a 3D DNA origami structure as fiducial for tip reconstruction and image correction. Our fiducial is stable under a broad range of conditions and has sharp steps at different heights that enable reliable tip reconstruction from as few as ten fiducials. The DNA origami is readily codeposited with biological and nonbiological samples, achieves higher precision for the tip apex than polycrystalline samples, and dramatically improves the accuracy of the lateral dimensions determined from the images. Our fiducial thus enables accurate and precise AFM imaging for a broad range of applications.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>36745573</pmid><doi>10.1021/acs.nanolett.2c04299</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-5239-3043</orcidid><orcidid>https://orcid.org/0000-0002-0040-0173</orcidid><orcidid>https://orcid.org/0000-0001-9422-8062</orcidid><orcidid>https://orcid.org/0000-0003-3613-7896</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1530-6984 |
| ispartof | Nano letters, 2023-02, Vol.23 (4), p.1236-1243 |
| issn | 1530-6984 1530-6992 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9951250 |
| source | MEDLINE; ACS Publications |
| subjects | DNA - chemistry Letter Microscopy, Atomic Force - methods Nanoparticles |
| title | DNA Origami Fiducial for Accurate 3D Atomic Force Microscopy Imaging |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T17%3A28%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DNA%20Origami%20Fiducial%20for%20Accurate%203D%20Atomic%20Force%20Microscopy%20Imaging&rft.jtitle=Nano%20letters&rft.au=Kolbeck,%20Pauline%20J.&rft.date=2023-02-22&rft.volume=23&rft.issue=4&rft.spage=1236&rft.epage=1243&rft.pages=1236-1243&rft.issn=1530-6984&rft.eissn=1530-6992&rft_id=info:doi/10.1021/acs.nanolett.2c04299&rft_dat=%3Cproquest_pubme%3E2773714117%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2773714117&rft_id=info:pmid/36745573&rfr_iscdi=true |