Temperature dependent mechanical unfolding and refolding of a protein studied by thermo-regulated optical tweezers

Temperature is a useful system variable to gather kinetic and thermodynamic information from proteins. Usually, free energy and the associated entropic and enthalpic contributions are obtained by quantifying the conformational equilibrium based on melting experiments performed in bulk conditions. Su...

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Veröffentlicht in:	Biophysical journal 2023-02, Vol.122 (3), p.513-521
Hauptverfasser:	Rivera, Maira, Mjaavatten, Are, Smith, Steven B., Baez, Mauricio, Wilson, Christian A.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Entropy Kinetics Optical Tweezers Protein Denaturation Protein Folding Temperature Thermodynamics
Online-Zugang:	Volltext
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