A LuALS Mutation with High Sulfonylurea Herbicide Resistance in Linum usitatissimum L
The cultivation of herbicide-resistant crops is an effective tool for weed management in agriculture. Weed control in flax ( L.) remains challenging due to the lack of available herbicide-resistant cultivars. In this study, a mutant resistant to acetolactate synthase (ALS)-inhibiting herbicides was...
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| Veröffentlicht in: | International journal of molecular sciences 2023-02, Vol.24 (3), p.2820 |
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| creator | Liu, Caiyue Zhang, Tianbao Yang, Xinsen Wang, Liu Long, Yan Hasi, Agula Pei, Xinwu |
| description | The cultivation of herbicide-resistant crops is an effective tool for weed management in agriculture. Weed control in flax (
L.) remains challenging due to the lack of available herbicide-resistant cultivars. In this study, a mutant resistant to acetolactate synthase (ALS)-inhibiting herbicides was obtained by ethyl methanesulphonate (EMS) mutagenesis using an elite cultivar, Longya10. Whole-plant dose-response assays revealed that, compared to Longya10, the mutant was 11.57-fold more resistant to tribenuron-methyl (TBM) and slightly resistant to imazethapyr (resistance index (mutant/Longya10) < 3). In vitro acetolactate synthase assays showed that the relative resistance of the mutant was 12.63 times more than that of Longya10. A biochemical analysis indicated that there was a Pro197Ser (relative to the
ALS sequence) substitution within the LuALS1, conferring high resistance to sulfonylurea herbicides in the mutant. Additionally, two cleaved amplified polymorphic sequence (CAPS) markers,
I-
and
O109I-
, were developed based on the mutation site for marker assistant selection in breeding. Moreover, the mutant did not cause losses in natural field conditions. We find a mutant with ALS-inhibiting herbicide resistance chemically induced by EMS mutagenesis, providing a valuable germplasm for breeding herbicide-resistant flax varieties. |
| doi_str_mv | 10.3390/ijms24032820 |
| format | Article |
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L.) remains challenging due to the lack of available herbicide-resistant cultivars. In this study, a mutant resistant to acetolactate synthase (ALS)-inhibiting herbicides was obtained by ethyl methanesulphonate (EMS) mutagenesis using an elite cultivar, Longya10. Whole-plant dose-response assays revealed that, compared to Longya10, the mutant was 11.57-fold more resistant to tribenuron-methyl (TBM) and slightly resistant to imazethapyr (resistance index (mutant/Longya10) < 3). In vitro acetolactate synthase assays showed that the relative resistance of the mutant was 12.63 times more than that of Longya10. A biochemical analysis indicated that there was a Pro197Ser (relative to the
ALS sequence) substitution within the LuALS1, conferring high resistance to sulfonylurea herbicides in the mutant. Additionally, two cleaved amplified polymorphic sequence (CAPS) markers,
I-
and
O109I-
, were developed based on the mutation site for marker assistant selection in breeding. Moreover, the mutant did not cause losses in natural field conditions. We find a mutant with ALS-inhibiting herbicide resistance chemically induced by EMS mutagenesis, providing a valuable germplasm for breeding herbicide-resistant flax varieties.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms24032820</identifier><identifier>PMID: 36769141</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Acetolactate synthase ; Acetolactate Synthase - genetics ; Agricultural production ; Arabidopsis ; Biochemical analysis ; Cultivars ; Ethyl methanesulfonate ; Flax ; Flax - genetics ; Germplasm ; Herbicide resistance ; Herbicide Resistance - genetics ; Herbicides ; Herbicides - pharmacology ; High resistance ; Linum usitatissimum ; Mutagenesis ; Mutants ; Mutation ; Plant Breeding ; Sulfonylurea ; Sulfonylurea Compounds - pharmacology ; Weed control ; Weeds</subject><ispartof>International journal of molecular sciences, 2023-02, Vol.24 (3), p.2820</ispartof><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-78c3512c24673a4ebdf529f912812673f46fd5b8f90ccb9835c2b44c97c929fc3</citedby><cites>FETCH-LOGICAL-c412t-78c3512c24673a4ebdf529f912812673f46fd5b8f90ccb9835c2b44c97c929fc3</cites><orcidid>0000-0001-7828-3037</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917167/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917167/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36769141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Caiyue</creatorcontrib><creatorcontrib>Zhang, Tianbao</creatorcontrib><creatorcontrib>Yang, Xinsen</creatorcontrib><creatorcontrib>Wang, Liu</creatorcontrib><creatorcontrib>Long, Yan</creatorcontrib><creatorcontrib>Hasi, Agula</creatorcontrib><creatorcontrib>Pei, Xinwu</creatorcontrib><title>A LuALS Mutation with High Sulfonylurea Herbicide Resistance in Linum usitatissimum L</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>The cultivation of herbicide-resistant crops is an effective tool for weed management in agriculture. Weed control in flax (
L.) remains challenging due to the lack of available herbicide-resistant cultivars. In this study, a mutant resistant to acetolactate synthase (ALS)-inhibiting herbicides was obtained by ethyl methanesulphonate (EMS) mutagenesis using an elite cultivar, Longya10. Whole-plant dose-response assays revealed that, compared to Longya10, the mutant was 11.57-fold more resistant to tribenuron-methyl (TBM) and slightly resistant to imazethapyr (resistance index (mutant/Longya10) < 3). In vitro acetolactate synthase assays showed that the relative resistance of the mutant was 12.63 times more than that of Longya10. A biochemical analysis indicated that there was a Pro197Ser (relative to the
ALS sequence) substitution within the LuALS1, conferring high resistance to sulfonylurea herbicides in the mutant. Additionally, two cleaved amplified polymorphic sequence (CAPS) markers,
I-
and
O109I-
, were developed based on the mutation site for marker assistant selection in breeding. Moreover, the mutant did not cause losses in natural field conditions. We find a mutant with ALS-inhibiting herbicide resistance chemically induced by EMS mutagenesis, providing a valuable germplasm for breeding herbicide-resistant flax varieties.</description><subject>Acetolactate synthase</subject><subject>Acetolactate Synthase - genetics</subject><subject>Agricultural production</subject><subject>Arabidopsis</subject><subject>Biochemical analysis</subject><subject>Cultivars</subject><subject>Ethyl methanesulfonate</subject><subject>Flax</subject><subject>Flax - genetics</subject><subject>Germplasm</subject><subject>Herbicide resistance</subject><subject>Herbicide Resistance - genetics</subject><subject>Herbicides</subject><subject>Herbicides - pharmacology</subject><subject>High resistance</subject><subject>Linum usitatissimum</subject><subject>Mutagenesis</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Plant Breeding</subject><subject>Sulfonylurea</subject><subject>Sulfonylurea Compounds - pharmacology</subject><subject>Weed control</subject><subject>Weeds</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkctLwzAcx4MoPqY3zxLw4sFpXm2aizCGOqEiOHcOaZa6jDbVpFH235vhlOnp9_r8XnwBOMXoilKBru2yDYQhSgqCdsAhZoQMEcr57pZ_AI5CWCJEKMnEPjigOc8FZvgQzEawjKNyCh9jr3rbOfhp-wWc2NcFnMam7tyqid4oODG-strODXw2wYZeOW2gdbC0LrYwBrtuD8G2KSqPwV6tmmBONnYAZne3L-PJsHy6fxiPyqFmmPRDXmiaYaIJyzlVzFTzOiOiFpgUmKRUzfJ6nlVFLZDWlShopknFmBZci8RpOgA333PfYtWauTau96qRb962yq9kp6z8W3F2IV-7DykE5jhtGICLzQDfvUcTetnaoE3TKGe6GCThPMtJxliR0PN_6LKL3qX31hRLRyeTqMtvSvsuBG_q32Mwkmu95LZeCT_bfuAX_hGIfgFcbZEH</recordid><startdate>20230201</startdate><enddate>20230201</enddate><creator>Liu, Caiyue</creator><creator>Zhang, Tianbao</creator><creator>Yang, Xinsen</creator><creator>Wang, Liu</creator><creator>Long, Yan</creator><creator>Hasi, Agula</creator><creator>Pei, Xinwu</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7828-3037</orcidid></search><sort><creationdate>20230201</creationdate><title>A LuALS Mutation with High Sulfonylurea Herbicide Resistance in Linum usitatissimum L</title><author>Liu, Caiyue ; Zhang, Tianbao ; Yang, Xinsen ; Wang, Liu ; Long, Yan ; Hasi, Agula ; Pei, Xinwu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-78c3512c24673a4ebdf529f912812673f46fd5b8f90ccb9835c2b44c97c929fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Acetolactate synthase</topic><topic>Acetolactate Synthase - genetics</topic><topic>Agricultural production</topic><topic>Arabidopsis</topic><topic>Biochemical analysis</topic><topic>Cultivars</topic><topic>Ethyl methanesulfonate</topic><topic>Flax</topic><topic>Flax - genetics</topic><topic>Germplasm</topic><topic>Herbicide resistance</topic><topic>Herbicide Resistance - genetics</topic><topic>Herbicides</topic><topic>Herbicides - pharmacology</topic><topic>High resistance</topic><topic>Linum usitatissimum</topic><topic>Mutagenesis</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Plant Breeding</topic><topic>Sulfonylurea</topic><topic>Sulfonylurea Compounds - pharmacology</topic><topic>Weed control</topic><topic>Weeds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Caiyue</creatorcontrib><creatorcontrib>Zhang, Tianbao</creatorcontrib><creatorcontrib>Yang, Xinsen</creatorcontrib><creatorcontrib>Wang, Liu</creatorcontrib><creatorcontrib>Long, Yan</creatorcontrib><creatorcontrib>Hasi, Agula</creatorcontrib><creatorcontrib>Pei, Xinwu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Caiyue</au><au>Zhang, Tianbao</au><au>Yang, Xinsen</au><au>Wang, Liu</au><au>Long, Yan</au><au>Hasi, Agula</au><au>Pei, Xinwu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A LuALS Mutation with High Sulfonylurea Herbicide Resistance in Linum usitatissimum L</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2023-02-01</date><risdate>2023</risdate><volume>24</volume><issue>3</issue><spage>2820</spage><pages>2820-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>The cultivation of herbicide-resistant crops is an effective tool for weed management in agriculture. Weed control in flax (
L.) remains challenging due to the lack of available herbicide-resistant cultivars. In this study, a mutant resistant to acetolactate synthase (ALS)-inhibiting herbicides was obtained by ethyl methanesulphonate (EMS) mutagenesis using an elite cultivar, Longya10. Whole-plant dose-response assays revealed that, compared to Longya10, the mutant was 11.57-fold more resistant to tribenuron-methyl (TBM) and slightly resistant to imazethapyr (resistance index (mutant/Longya10) < 3). In vitro acetolactate synthase assays showed that the relative resistance of the mutant was 12.63 times more than that of Longya10. A biochemical analysis indicated that there was a Pro197Ser (relative to the
ALS sequence) substitution within the LuALS1, conferring high resistance to sulfonylurea herbicides in the mutant. Additionally, two cleaved amplified polymorphic sequence (CAPS) markers,
I-
and
O109I-
, were developed based on the mutation site for marker assistant selection in breeding. Moreover, the mutant did not cause losses in natural field conditions. We find a mutant with ALS-inhibiting herbicide resistance chemically induced by EMS mutagenesis, providing a valuable germplasm for breeding herbicide-resistant flax varieties.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>36769141</pmid><doi>10.3390/ijms24032820</doi><orcidid>https://orcid.org/0000-0001-7828-3037</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acetolactate synthase Acetolactate Synthase - genetics Agricultural production Arabidopsis Biochemical analysis Cultivars Ethyl methanesulfonate Flax Flax - genetics Germplasm Herbicide resistance Herbicide Resistance - genetics Herbicides Herbicides - pharmacology High resistance Linum usitatissimum Mutagenesis Mutants Mutation Plant Breeding Sulfonylurea Sulfonylurea Compounds - pharmacology Weed control Weeds |
| title | A LuALS Mutation with High Sulfonylurea Herbicide Resistance in Linum usitatissimum L |
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