Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes
Induced pluripotent stem cells (iPSCs) can differentiate into all types of cells and can be used in livestock for research on biological development, genetic breeding, and in vitro genetic resource conservation. The Bactrian camel is a large domestic animal that inhabits extreme environments and hol...
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| Veröffentlicht in: | International journal of molecular sciences 2023-01, Vol.24 (3), p.1917 |
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| creator | Li, Zongshuai Li, Yina Zhang, Qiran Ge, Wenbo Zhang, Yong Zhao, Xingxu Hu, Junjie Yuan, Ligang Zhang, Wangdong |
| description | Induced pluripotent stem cells (iPSCs) can differentiate into all types of cells and can be used in livestock for research on biological development, genetic breeding, and in vitro genetic resource conservation. The Bactrian camel is a large domestic animal that inhabits extreme environments and holds value in the treatment of various diseases and the development of the local economy. Therefore, we transferred four mouse genes (
,
,
, and c-
) into Bactrian camel fetal fibroblasts (BCFFs) using retroviruses with a large host range to obtain Bactrian camel induced pluripotent stem cells (bciPSCs). They were comprehensively identified based on cell morphology, pluripotency gene and marker expression, chromosome number, transcriptome sequencing, and differentiation potential. The results showed the pluripotency of bciPSCs. However, unlike stem cells of other species, late formation of stem cell clones was observed; moreover, the immunofluorescence of SSEA1, SSEA3, and SSEA4 were positive, and teratoma formation took four months. These findings may be related to the extremely long gestation period and species specificity of Bactrian camels. By mining RNA sequence data, 85 potential unique pluripotent genes of Bactrian camels were predicted, which could be used as candidate genes for the production of bciPSC in the future. Among them,
,
,
,
,
,
,
, and
are more attractive. In conclusion, we generated bciPSCs for the first time and obtained their transcriptome information, expanding the iPSC genetic information database and exploring the applicability of iPSCs in livestock. Our results can provide an experimental basis for Bactrian camel ESC establishment, developmental research, and genetic resource conservation. |
| doi_str_mv | 10.3390/ijms24031917 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9916525</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2775617823</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-9ca29c5ec441e83b6503c98773a872ab634652109c26d25350a15da46c24f85f3</originalsourceid><addsrcrecordid>eNpdkUtLAzEUhYMovneuJeDGhdW8M9kIWnxBQUFdhzSTsSkzSU1mBP-9KVapru6F-53DPRwAjjA6p1ShCz_vMmGIYoXlBtjFjJARQkJuru07YC_nOUKEEq62wQ4VUlRFtAsWN7k309bnWedCD2MDr43tkzcBjk3nWvgQ6sG6Gj61Q_KL2C-p5951cOzaNkMTyim52tvex7DUv8ycT_A1-PfBransJ7xzweUDsNWYNrvD1dwHr7c3L-P70eTx7mF8NRlZhkk_UtYQZbmzjGFX0angiFpVSUlNJYmZCsoEJxgpS0RNOOXIYF4bJixhTcUbug8uv30Xw7RztS1_J9PqRfKdSZ86Gq__XoKf6bf4oZXCxZkXg9OVQYolSu5157MtoU1wcciaSMkFlhWhBT35h87jkEKJt6RYMVRUFersm7Ip5pxc8_sMRnpZpV6vsuDH6wF-4Z_u6BeOdJq4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2774916939</pqid></control><display><type>article</type><title>Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>PubMed Central</source><creator>Li, Zongshuai ; Li, Yina ; Zhang, Qiran ; Ge, Wenbo ; Zhang, Yong ; Zhao, Xingxu ; Hu, Junjie ; Yuan, Ligang ; Zhang, Wangdong</creator><creatorcontrib>Li, Zongshuai ; Li, Yina ; Zhang, Qiran ; Ge, Wenbo ; Zhang, Yong ; Zhao, Xingxu ; Hu, Junjie ; Yuan, Ligang ; Zhang, Wangdong</creatorcontrib><description>Induced pluripotent stem cells (iPSCs) can differentiate into all types of cells and can be used in livestock for research on biological development, genetic breeding, and in vitro genetic resource conservation. The Bactrian camel is a large domestic animal that inhabits extreme environments and holds value in the treatment of various diseases and the development of the local economy. Therefore, we transferred four mouse genes (
,
,
, and c-
) into Bactrian camel fetal fibroblasts (BCFFs) using retroviruses with a large host range to obtain Bactrian camel induced pluripotent stem cells (bciPSCs). They were comprehensively identified based on cell morphology, pluripotency gene and marker expression, chromosome number, transcriptome sequencing, and differentiation potential. The results showed the pluripotency of bciPSCs. However, unlike stem cells of other species, late formation of stem cell clones was observed; moreover, the immunofluorescence of SSEA1, SSEA3, and SSEA4 were positive, and teratoma formation took four months. These findings may be related to the extremely long gestation period and species specificity of Bactrian camels. By mining RNA sequence data, 85 potential unique pluripotent genes of Bactrian camels were predicted, which could be used as candidate genes for the production of bciPSC in the future. Among them,
,
,
,
,
,
,
, and
are more attractive. In conclusion, we generated bciPSCs for the first time and obtained their transcriptome information, expanding the iPSC genetic information database and exploring the applicability of iPSCs in livestock. Our results can provide an experimental basis for Bactrian camel ESC establishment, developmental research, and genetic resource conservation.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms24031917</identifier><identifier>PMID: 36768240</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; Animals, Domestic - metabolism ; Apoptosis ; Breeding ; c-Myc protein ; Camelidae ; Camelus - genetics ; Camelus bactrianus ; Cell cycle ; Cell differentiation ; Cell Differentiation - genetics ; Chromosome number ; Connective tissue ; Conservation ; Cytokines - metabolism ; Cytology ; Evolution & development ; Extreme environments ; Fetuses ; Gene expression ; Genes ; Genomes ; Hogs ; Host range ; Immunofluorescence ; Induced Pluripotent Stem Cells ; KLF4 protein ; Lewis X Antigen - metabolism ; Livestock ; Mice ; Morphology ; Myc protein ; Nuclear Pore Complex Proteins - metabolism ; Nucleotide sequence ; Oct-4 protein ; Phosphorylation ; Plasmids ; Pluripotency ; Proteins ; Resource conservation ; Stem cell transplantation ; Stem cells ; Telomerase ; Teratoma ; Transcriptomes ; Tumors</subject><ispartof>International journal of molecular sciences, 2023-01, Vol.24 (3), p.1917</ispartof><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-9ca29c5ec441e83b6503c98773a872ab634652109c26d25350a15da46c24f85f3</citedby><cites>FETCH-LOGICAL-c412t-9ca29c5ec441e83b6503c98773a872ab634652109c26d25350a15da46c24f85f3</cites><orcidid>0000-0003-2522-4724 ; 0000-0002-8386-0262</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916525/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916525/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,27913,27914,53780,53782</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36768240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Zongshuai</creatorcontrib><creatorcontrib>Li, Yina</creatorcontrib><creatorcontrib>Zhang, Qiran</creatorcontrib><creatorcontrib>Ge, Wenbo</creatorcontrib><creatorcontrib>Zhang, Yong</creatorcontrib><creatorcontrib>Zhao, Xingxu</creatorcontrib><creatorcontrib>Hu, Junjie</creatorcontrib><creatorcontrib>Yuan, Ligang</creatorcontrib><creatorcontrib>Zhang, Wangdong</creatorcontrib><title>Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Induced pluripotent stem cells (iPSCs) can differentiate into all types of cells and can be used in livestock for research on biological development, genetic breeding, and in vitro genetic resource conservation. The Bactrian camel is a large domestic animal that inhabits extreme environments and holds value in the treatment of various diseases and the development of the local economy. Therefore, we transferred four mouse genes (
,
,
, and c-
) into Bactrian camel fetal fibroblasts (BCFFs) using retroviruses with a large host range to obtain Bactrian camel induced pluripotent stem cells (bciPSCs). They were comprehensively identified based on cell morphology, pluripotency gene and marker expression, chromosome number, transcriptome sequencing, and differentiation potential. The results showed the pluripotency of bciPSCs. However, unlike stem cells of other species, late formation of stem cell clones was observed; moreover, the immunofluorescence of SSEA1, SSEA3, and SSEA4 were positive, and teratoma formation took four months. These findings may be related to the extremely long gestation period and species specificity of Bactrian camels. By mining RNA sequence data, 85 potential unique pluripotent genes of Bactrian camels were predicted, which could be used as candidate genes for the production of bciPSC in the future. Among them,
,
,
,
,
,
,
, and
are more attractive. In conclusion, we generated bciPSCs for the first time and obtained their transcriptome information, expanding the iPSC genetic information database and exploring the applicability of iPSCs in livestock. Our results can provide an experimental basis for Bactrian camel ESC establishment, developmental research, and genetic resource conservation.</description><subject>Animals</subject><subject>Animals, Domestic - metabolism</subject><subject>Apoptosis</subject><subject>Breeding</subject><subject>c-Myc protein</subject><subject>Camelidae</subject><subject>Camelus - genetics</subject><subject>Camelus bactrianus</subject><subject>Cell cycle</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - genetics</subject><subject>Chromosome number</subject><subject>Connective tissue</subject><subject>Conservation</subject><subject>Cytokines - metabolism</subject><subject>Cytology</subject><subject>Evolution & development</subject><subject>Extreme environments</subject><subject>Fetuses</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genomes</subject><subject>Hogs</subject><subject>Host range</subject><subject>Immunofluorescence</subject><subject>Induced Pluripotent Stem Cells</subject><subject>KLF4 protein</subject><subject>Lewis X Antigen - metabolism</subject><subject>Livestock</subject><subject>Mice</subject><subject>Morphology</subject><subject>Myc protein</subject><subject>Nuclear Pore Complex Proteins - metabolism</subject><subject>Nucleotide sequence</subject><subject>Oct-4 protein</subject><subject>Phosphorylation</subject><subject>Plasmids</subject><subject>Pluripotency</subject><subject>Proteins</subject><subject>Resource conservation</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Telomerase</subject><subject>Teratoma</subject><subject>Transcriptomes</subject><subject>Tumors</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkUtLAzEUhYMovneuJeDGhdW8M9kIWnxBQUFdhzSTsSkzSU1mBP-9KVapru6F-53DPRwAjjA6p1ShCz_vMmGIYoXlBtjFjJARQkJuru07YC_nOUKEEq62wQ4VUlRFtAsWN7k309bnWedCD2MDr43tkzcBjk3nWvgQ6sG6Gj61Q_KL2C-p5951cOzaNkMTyim52tvex7DUv8ycT_A1-PfBransJ7xzweUDsNWYNrvD1dwHr7c3L-P70eTx7mF8NRlZhkk_UtYQZbmzjGFX0angiFpVSUlNJYmZCsoEJxgpS0RNOOXIYF4bJixhTcUbug8uv30Xw7RztS1_J9PqRfKdSZ86Gq__XoKf6bf4oZXCxZkXg9OVQYolSu5157MtoU1wcciaSMkFlhWhBT35h87jkEKJt6RYMVRUFersm7Ip5pxc8_sMRnpZpV6vsuDH6wF-4Z_u6BeOdJq4</recordid><startdate>20230118</startdate><enddate>20230118</enddate><creator>Li, Zongshuai</creator><creator>Li, Yina</creator><creator>Zhang, Qiran</creator><creator>Ge, Wenbo</creator><creator>Zhang, Yong</creator><creator>Zhao, Xingxu</creator><creator>Hu, Junjie</creator><creator>Yuan, Ligang</creator><creator>Zhang, Wangdong</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2522-4724</orcidid><orcidid>https://orcid.org/0000-0002-8386-0262</orcidid></search><sort><creationdate>20230118</creationdate><title>Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes</title><author>Li, Zongshuai ; Li, Yina ; Zhang, Qiran ; Ge, Wenbo ; Zhang, Yong ; Zhao, Xingxu ; Hu, Junjie ; Yuan, Ligang ; Zhang, Wangdong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-9ca29c5ec441e83b6503c98773a872ab634652109c26d25350a15da46c24f85f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Animals, Domestic - metabolism</topic><topic>Apoptosis</topic><topic>Breeding</topic><topic>c-Myc protein</topic><topic>Camelidae</topic><topic>Camelus - genetics</topic><topic>Camelus bactrianus</topic><topic>Cell cycle</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - genetics</topic><topic>Chromosome number</topic><topic>Connective tissue</topic><topic>Conservation</topic><topic>Cytokines - metabolism</topic><topic>Cytology</topic><topic>Evolution & development</topic><topic>Extreme environments</topic><topic>Fetuses</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genomes</topic><topic>Hogs</topic><topic>Host range</topic><topic>Immunofluorescence</topic><topic>Induced Pluripotent Stem Cells</topic><topic>KLF4 protein</topic><topic>Lewis X Antigen - metabolism</topic><topic>Livestock</topic><topic>Mice</topic><topic>Morphology</topic><topic>Myc protein</topic><topic>Nuclear Pore Complex Proteins - metabolism</topic><topic>Nucleotide sequence</topic><topic>Oct-4 protein</topic><topic>Phosphorylation</topic><topic>Plasmids</topic><topic>Pluripotency</topic><topic>Proteins</topic><topic>Resource conservation</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Telomerase</topic><topic>Teratoma</topic><topic>Transcriptomes</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Zongshuai</creatorcontrib><creatorcontrib>Li, Yina</creatorcontrib><creatorcontrib>Zhang, Qiran</creatorcontrib><creatorcontrib>Ge, Wenbo</creatorcontrib><creatorcontrib>Zhang, Yong</creatorcontrib><creatorcontrib>Zhao, Xingxu</creatorcontrib><creatorcontrib>Hu, Junjie</creatorcontrib><creatorcontrib>Yuan, Ligang</creatorcontrib><creatorcontrib>Zhang, Wangdong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zongshuai</au><au>Li, Yina</au><au>Zhang, Qiran</au><au>Ge, Wenbo</au><au>Zhang, Yong</au><au>Zhao, Xingxu</au><au>Hu, Junjie</au><au>Yuan, Ligang</au><au>Zhang, Wangdong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2023-01-18</date><risdate>2023</risdate><volume>24</volume><issue>3</issue><spage>1917</spage><pages>1917-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Induced pluripotent stem cells (iPSCs) can differentiate into all types of cells and can be used in livestock for research on biological development, genetic breeding, and in vitro genetic resource conservation. The Bactrian camel is a large domestic animal that inhabits extreme environments and holds value in the treatment of various diseases and the development of the local economy. Therefore, we transferred four mouse genes (
,
,
, and c-
) into Bactrian camel fetal fibroblasts (BCFFs) using retroviruses with a large host range to obtain Bactrian camel induced pluripotent stem cells (bciPSCs). They were comprehensively identified based on cell morphology, pluripotency gene and marker expression, chromosome number, transcriptome sequencing, and differentiation potential. The results showed the pluripotency of bciPSCs. However, unlike stem cells of other species, late formation of stem cell clones was observed; moreover, the immunofluorescence of SSEA1, SSEA3, and SSEA4 were positive, and teratoma formation took four months. These findings may be related to the extremely long gestation period and species specificity of Bactrian camels. By mining RNA sequence data, 85 potential unique pluripotent genes of Bactrian camels were predicted, which could be used as candidate genes for the production of bciPSC in the future. Among them,
,
,
,
,
,
,
, and
are more attractive. In conclusion, we generated bciPSCs for the first time and obtained their transcriptome information, expanding the iPSC genetic information database and exploring the applicability of iPSCs in livestock. Our results can provide an experimental basis for Bactrian camel ESC establishment, developmental research, and genetic resource conservation.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>36768240</pmid><doi>10.3390/ijms24031917</doi><orcidid>https://orcid.org/0000-0003-2522-4724</orcidid><orcidid>https://orcid.org/0000-0002-8386-0262</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of molecular sciences, 2023-01, Vol.24 (3), p.1917 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Animals Animals, Domestic - metabolism Apoptosis Breeding c-Myc protein Camelidae Camelus - genetics Camelus bactrianus Cell cycle Cell differentiation Cell Differentiation - genetics Chromosome number Connective tissue Conservation Cytokines - metabolism Cytology Evolution & development Extreme environments Fetuses Gene expression Genes Genomes Hogs Host range Immunofluorescence Induced Pluripotent Stem Cells KLF4 protein Lewis X Antigen - metabolism Livestock Mice Morphology Myc protein Nuclear Pore Complex Proteins - metabolism Nucleotide sequence Oct-4 protein Phosphorylation Plasmids Pluripotency Proteins Resource conservation Stem cell transplantation Stem cells Telomerase Teratoma Transcriptomes Tumors |
| title | Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T08%3A14%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Establishment%20of%20Bactrian%20Camel%20Induced%20Pluripotent%20Stem%20Cells%20and%20Prediction%20of%20Their%20Unique%20Pluripotency%20Genes&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Li,%20Zongshuai&rft.date=2023-01-18&rft.volume=24&rft.issue=3&rft.spage=1917&rft.pages=1917-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms24031917&rft_dat=%3Cproquest_pubme%3E2775617823%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2774916939&rft_id=info:pmid/36768240&rfr_iscdi=true |