Holistic approach to visualize and quantify collagen organization at macro, micro, and nano‐scale
Background There is scarcity of imaging and image processing techniques for accurate discrimination and quantitation of the dermal extracellular matrix (ECM), primarily collagen. The aim of this study was to develop and demonstrate a holistic imaging and image processing approach to visualize and qu...
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| Veröffentlicht in: | Skin research and technology 2022-05, Vol.28 (3), p.419-426 |
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| creator | Bhardwaj, Vinay Fabijanic, Kristina Ivana Cohen, Aaron Mao, Junhong Azadegan, Chloe Pittet, Jean Christophe Bris, Benedicte Le |
| description | Background
There is scarcity of imaging and image processing techniques for accurate discrimination and quantitation of the dermal extracellular matrix (ECM), primarily collagen. The aim of this study was to develop and demonstrate a holistic imaging and image processing approach to visualize and quantify collagen remodeling at the macro‐, micro‐ and nano‐scale using histochemical imaging, Reflectance Confocal Microscopy (RCM), and Atomic Force Microscopy (AFM), respectively.
Material and Methods
For proof‐of‐concept, a commercial anti‐aging product known to induce collagen neo‐synthesis and re‐organization was tested ex vivo on human skin biopsies from two aged females.
Results
Relative to untreated skin, collagen fibers (RCM) and fibrils (AFM) were longer and aligned after treatment. The content of collagen and elastin (histochemical imaging and ELISA) statistically improved after treatment.
Conclusion
Based on our findings, we can conclude: (1) AFM, RCM, and histochemical imaging can accurately discriminate collagen from other ECM components in the skin and (2) the image processing methods can enable quantitation and hence capture small improvements in collagen remodeling after treatment (commercial cosmetic product with collagen organizer technology as proof‐of‐concept). The reported holistic imaging approach has direct clinical implications for scientists and dermatologists to make quick, real‐time, and accurate decisions in skin research and diagnostics. |
| doi_str_mv | 10.1111/srt.13140 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9907653</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2638942093</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4430-1f6b60fce0c71dba4a20328564ede8aeb12ad93a1958804440471f657b023313</originalsourceid><addsrcrecordid>eNp1kcFu1DAQhi0EokvhwAsgS1xAIu04tpP4UglVlCJVqgR74GZNHGfrKrG3dlK0PfEIPGOfBG-3VBSJucxhvvn1z_yEvGZwwHIdpjgdMM4EPCELVgEU0IjqKVmAAlXUsvy-R16kdAkAUjH-nOxxWTZSynJBzGkYXJqcobhex4Dmgk6BXrs04-BuLEXf0asZ_eT6DTVhGHBlPQ1xhd7d4OSCpzjREU0MH-jo7tp2x6MPtz9_JYODfUme9Tgk--q-75Plyafl8Wlxdv75y_HHs8IIwaFgfdVW0BsLpmZdiwJL4NlnJWxnG7QtK7FTHJmSTQNCCBB13pF1CyXnjO-To53sem5H2xnrp4iDXkc3YtzogE4_nnh3oVfhWisFdSV5Fnh3LxDD1WzTpEeXjM03exvmpMuKN0qUoLbo23_QyzBHn6_LVKUEA1nXmXq_o_JbUoq2fzDDQG-T0zk5fZdcZt_87f6B_BNVBg53wA832M3_lfS3r8ud5G_oK6SC</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2669410577</pqid></control><display><type>article</type><title>Holistic approach to visualize and quantify collagen organization at macro, micro, and nano‐scale</title><source>MEDLINE</source><source>Wiley Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Wiley-Blackwell Open Access Titles</source><source>PubMed Central</source><creator>Bhardwaj, Vinay ; Fabijanic, Kristina Ivana ; Cohen, Aaron ; Mao, Junhong ; Azadegan, Chloe ; Pittet, Jean Christophe ; Bris, Benedicte Le</creator><creatorcontrib>Bhardwaj, Vinay ; Fabijanic, Kristina Ivana ; Cohen, Aaron ; Mao, Junhong ; Azadegan, Chloe ; Pittet, Jean Christophe ; Bris, Benedicte Le</creatorcontrib><description>Background
There is scarcity of imaging and image processing techniques for accurate discrimination and quantitation of the dermal extracellular matrix (ECM), primarily collagen. The aim of this study was to develop and demonstrate a holistic imaging and image processing approach to visualize and quantify collagen remodeling at the macro‐, micro‐ and nano‐scale using histochemical imaging, Reflectance Confocal Microscopy (RCM), and Atomic Force Microscopy (AFM), respectively.
Material and Methods
For proof‐of‐concept, a commercial anti‐aging product known to induce collagen neo‐synthesis and re‐organization was tested ex vivo on human skin biopsies from two aged females.
Results
Relative to untreated skin, collagen fibers (RCM) and fibrils (AFM) were longer and aligned after treatment. The content of collagen and elastin (histochemical imaging and ELISA) statistically improved after treatment.
Conclusion
Based on our findings, we can conclude: (1) AFM, RCM, and histochemical imaging can accurately discriminate collagen from other ECM components in the skin and (2) the image processing methods can enable quantitation and hence capture small improvements in collagen remodeling after treatment (commercial cosmetic product with collagen organizer technology as proof‐of‐concept). The reported holistic imaging approach has direct clinical implications for scientists and dermatologists to make quick, real‐time, and accurate decisions in skin research and diagnostics.</description><identifier>ISSN: 0909-752X</identifier><identifier>EISSN: 1600-0846</identifier><identifier>DOI: 10.1111/srt.13140</identifier><identifier>PMID: 35285552</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Aged ; Aging ; anti‐aging ; Atomic force microscopy ; Biopsy ; Collagen ; collagen reorganization ; Confocal microscopy ; dermal remodeling ; Elastin ; Extracellular Matrix ; Female ; Fibers ; Fibrils ; Humans ; Image processing ; Microscopy ; Microscopy, Confocal - methods ; Original ; Quantitation ; reflectance confocal microscopy (Vivascope) ; Skin ; Skin - diagnostic imaging</subject><ispartof>Skin research and technology, 2022-05, Vol.28 (3), p.419-426</ispartof><rights>2021 Colgate‐Palmolive Company. published by John Wiley & Sons Ltd.</rights><rights>2021 Colgate-Palmolive Company. Skin Research and Technology published by John Wiley & Sons Ltd.</rights><rights>2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4430-1f6b60fce0c71dba4a20328564ede8aeb12ad93a1958804440471f657b023313</citedby><cites>FETCH-LOGICAL-c4430-1f6b60fce0c71dba4a20328564ede8aeb12ad93a1958804440471f657b023313</cites><orcidid>0000-0001-7784-4541</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9907653/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9907653/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35285552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bhardwaj, Vinay</creatorcontrib><creatorcontrib>Fabijanic, Kristina Ivana</creatorcontrib><creatorcontrib>Cohen, Aaron</creatorcontrib><creatorcontrib>Mao, Junhong</creatorcontrib><creatorcontrib>Azadegan, Chloe</creatorcontrib><creatorcontrib>Pittet, Jean Christophe</creatorcontrib><creatorcontrib>Bris, Benedicte Le</creatorcontrib><title>Holistic approach to visualize and quantify collagen organization at macro, micro, and nano‐scale</title><title>Skin research and technology</title><addtitle>Skin Res Technol</addtitle><description>Background
There is scarcity of imaging and image processing techniques for accurate discrimination and quantitation of the dermal extracellular matrix (ECM), primarily collagen. The aim of this study was to develop and demonstrate a holistic imaging and image processing approach to visualize and quantify collagen remodeling at the macro‐, micro‐ and nano‐scale using histochemical imaging, Reflectance Confocal Microscopy (RCM), and Atomic Force Microscopy (AFM), respectively.
Material and Methods
For proof‐of‐concept, a commercial anti‐aging product known to induce collagen neo‐synthesis and re‐organization was tested ex vivo on human skin biopsies from two aged females.
Results
Relative to untreated skin, collagen fibers (RCM) and fibrils (AFM) were longer and aligned after treatment. The content of collagen and elastin (histochemical imaging and ELISA) statistically improved after treatment.
Conclusion
Based on our findings, we can conclude: (1) AFM, RCM, and histochemical imaging can accurately discriminate collagen from other ECM components in the skin and (2) the image processing methods can enable quantitation and hence capture small improvements in collagen remodeling after treatment (commercial cosmetic product with collagen organizer technology as proof‐of‐concept). The reported holistic imaging approach has direct clinical implications for scientists and dermatologists to make quick, real‐time, and accurate decisions in skin research and diagnostics.</description><subject>Aged</subject><subject>Aging</subject><subject>anti‐aging</subject><subject>Atomic force microscopy</subject><subject>Biopsy</subject><subject>Collagen</subject><subject>collagen reorganization</subject><subject>Confocal microscopy</subject><subject>dermal remodeling</subject><subject>Elastin</subject><subject>Extracellular Matrix</subject><subject>Female</subject><subject>Fibers</subject><subject>Fibrils</subject><subject>Humans</subject><subject>Image processing</subject><subject>Microscopy</subject><subject>Microscopy, Confocal - methods</subject><subject>Original</subject><subject>Quantitation</subject><subject>reflectance confocal microscopy (Vivascope)</subject><subject>Skin</subject><subject>Skin - diagnostic imaging</subject><issn>0909-752X</issn><issn>1600-0846</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kcFu1DAQhi0EokvhwAsgS1xAIu04tpP4UglVlCJVqgR74GZNHGfrKrG3dlK0PfEIPGOfBG-3VBSJucxhvvn1z_yEvGZwwHIdpjgdMM4EPCELVgEU0IjqKVmAAlXUsvy-R16kdAkAUjH-nOxxWTZSynJBzGkYXJqcobhex4Dmgk6BXrs04-BuLEXf0asZ_eT6DTVhGHBlPQ1xhd7d4OSCpzjREU0MH-jo7tp2x6MPtz9_JYODfUme9Tgk--q-75Plyafl8Wlxdv75y_HHs8IIwaFgfdVW0BsLpmZdiwJL4NlnJWxnG7QtK7FTHJmSTQNCCBB13pF1CyXnjO-To53sem5H2xnrp4iDXkc3YtzogE4_nnh3oVfhWisFdSV5Fnh3LxDD1WzTpEeXjM03exvmpMuKN0qUoLbo23_QyzBHn6_LVKUEA1nXmXq_o_JbUoq2fzDDQG-T0zk5fZdcZt_87f6B_BNVBg53wA832M3_lfS3r8ud5G_oK6SC</recordid><startdate>202205</startdate><enddate>202205</enddate><creator>Bhardwaj, Vinay</creator><creator>Fabijanic, Kristina Ivana</creator><creator>Cohen, Aaron</creator><creator>Mao, Junhong</creator><creator>Azadegan, Chloe</creator><creator>Pittet, Jean Christophe</creator><creator>Bris, Benedicte Le</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7X7</scope><scope>7XB</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7784-4541</orcidid></search><sort><creationdate>202205</creationdate><title>Holistic approach to visualize and quantify collagen organization at macro, micro, and nano‐scale</title><author>Bhardwaj, Vinay ; Fabijanic, Kristina Ivana ; Cohen, Aaron ; Mao, Junhong ; Azadegan, Chloe ; Pittet, Jean Christophe ; Bris, Benedicte Le</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4430-1f6b60fce0c71dba4a20328564ede8aeb12ad93a1958804440471f657b023313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aged</topic><topic>Aging</topic><topic>anti‐aging</topic><topic>Atomic force microscopy</topic><topic>Biopsy</topic><topic>Collagen</topic><topic>collagen reorganization</topic><topic>Confocal microscopy</topic><topic>dermal remodeling</topic><topic>Elastin</topic><topic>Extracellular Matrix</topic><topic>Female</topic><topic>Fibers</topic><topic>Fibrils</topic><topic>Humans</topic><topic>Image processing</topic><topic>Microscopy</topic><topic>Microscopy, Confocal - methods</topic><topic>Original</topic><topic>Quantitation</topic><topic>reflectance confocal microscopy (Vivascope)</topic><topic>Skin</topic><topic>Skin - diagnostic imaging</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhardwaj, Vinay</creatorcontrib><creatorcontrib>Fabijanic, Kristina Ivana</creatorcontrib><creatorcontrib>Cohen, Aaron</creatorcontrib><creatorcontrib>Mao, Junhong</creatorcontrib><creatorcontrib>Azadegan, Chloe</creatorcontrib><creatorcontrib>Pittet, Jean Christophe</creatorcontrib><creatorcontrib>Bris, Benedicte Le</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Skin research and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhardwaj, Vinay</au><au>Fabijanic, Kristina Ivana</au><au>Cohen, Aaron</au><au>Mao, Junhong</au><au>Azadegan, Chloe</au><au>Pittet, Jean Christophe</au><au>Bris, Benedicte Le</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Holistic approach to visualize and quantify collagen organization at macro, micro, and nano‐scale</atitle><jtitle>Skin research and technology</jtitle><addtitle>Skin Res Technol</addtitle><date>2022-05</date><risdate>2022</risdate><volume>28</volume><issue>3</issue><spage>419</spage><epage>426</epage><pages>419-426</pages><issn>0909-752X</issn><eissn>1600-0846</eissn><abstract>Background
There is scarcity of imaging and image processing techniques for accurate discrimination and quantitation of the dermal extracellular matrix (ECM), primarily collagen. The aim of this study was to develop and demonstrate a holistic imaging and image processing approach to visualize and quantify collagen remodeling at the macro‐, micro‐ and nano‐scale using histochemical imaging, Reflectance Confocal Microscopy (RCM), and Atomic Force Microscopy (AFM), respectively.
Material and Methods
For proof‐of‐concept, a commercial anti‐aging product known to induce collagen neo‐synthesis and re‐organization was tested ex vivo on human skin biopsies from two aged females.
Results
Relative to untreated skin, collagen fibers (RCM) and fibrils (AFM) were longer and aligned after treatment. The content of collagen and elastin (histochemical imaging and ELISA) statistically improved after treatment.
Conclusion
Based on our findings, we can conclude: (1) AFM, RCM, and histochemical imaging can accurately discriminate collagen from other ECM components in the skin and (2) the image processing methods can enable quantitation and hence capture small improvements in collagen remodeling after treatment (commercial cosmetic product with collagen organizer technology as proof‐of‐concept). The reported holistic imaging approach has direct clinical implications for scientists and dermatologists to make quick, real‐time, and accurate decisions in skin research and diagnostics.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>35285552</pmid><doi>10.1111/srt.13140</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-7784-4541</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Skin research and technology, 2022-05, Vol.28 (3), p.419-426 |
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| source | MEDLINE; Wiley Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley-Blackwell Open Access Titles; PubMed Central |
| subjects | Aged Aging anti‐aging Atomic force microscopy Biopsy Collagen collagen reorganization Confocal microscopy dermal remodeling Elastin Extracellular Matrix Female Fibers Fibrils Humans Image processing Microscopy Microscopy, Confocal - methods Original Quantitation reflectance confocal microscopy (Vivascope) Skin Skin - diagnostic imaging |
| title | Holistic approach to visualize and quantify collagen organization at macro, micro, and nano‐scale |
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