Phytochemical Screening, Antioxidant Potential, and LC–ESI–MS Profiling of Ephedra alata and Ephedra altissima Seeds Naturally Growing in Tunisia
Most Ephedra species are adapted to arid and desert conditions and are widely used in folk medicine to treat several disorders. The design of the current study was to determine the functional properties of seeds of two Ephedra species ( E. alata and E. altissima ) naturally growing in Tunisian arid...
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| creator | Mahmoudi, Maher Boughalleb, Fayçal Maaloul, Samah Mabrouk, Mahmoud Abdellaoui, Raoudha |
| description | Most
Ephedra
species are adapted to arid and desert conditions and are widely used in folk medicine to treat several disorders. The design of the current study was to determine the functional properties of seeds of two
Ephedra
species (
E. alata
and
E. altissima
) naturally growing in Tunisian arid zones by evaluating their mineral contents and bioactive compounds. The flame atomic absorption spectrometry revealed that seeds contained remarkable amounts of sodium (Na), potassium (K), calcium (Ca), magnesium (Mg), copper (Cu), zinc (Zn), and iron (Fe). The colorimetric investigation revealed high total polyphenol, flavonoid, and condensed tannin contents. Furthermore, by utilizing high-performance liquid chromatography-electrospray ionization–mass spectrometry method (HPLC-ESI/MS), a total of 11 phenolics were identified and quantified including 7 flavonoid compounds and 4 phenolic acids that were mostly predominated by gallic acid and quercetrin. Results so far have been very encouraging and proved that
Ephedra
seeds are a valuable source of natural bioactive compounds and minerals which could potentially be used for industrial and pharmaceutical purposes. |
| doi_str_mv | 10.1007/s12010-023-04370-8 |
| format | Article |
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Ephedra
species are adapted to arid and desert conditions and are widely used in folk medicine to treat several disorders. The design of the current study was to determine the functional properties of seeds of two
Ephedra
species (
E. alata
and
E. altissima
) naturally growing in Tunisian arid zones by evaluating their mineral contents and bioactive compounds. The flame atomic absorption spectrometry revealed that seeds contained remarkable amounts of sodium (Na), potassium (K), calcium (Ca), magnesium (Mg), copper (Cu), zinc (Zn), and iron (Fe). The colorimetric investigation revealed high total polyphenol, flavonoid, and condensed tannin contents. Furthermore, by utilizing high-performance liquid chromatography-electrospray ionization–mass spectrometry method (HPLC-ESI/MS), a total of 11 phenolics were identified and quantified including 7 flavonoid compounds and 4 phenolic acids that were mostly predominated by gallic acid and quercetrin. Results so far have been very encouraging and proved that
Ephedra
seeds are a valuable source of natural bioactive compounds and minerals which could potentially be used for industrial and pharmaceutical purposes.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-023-04370-8</identifier><identifier>PMID: 36719522</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>antioxidant activity ; Arid zones ; Aridity ; atomic absorption spectrometry ; Atomic absorption spectroscopy ; Bioactive compounds ; Biochemistry ; Biological activity ; Biotechnology ; Calcium ; Chemistry ; Chemistry and Materials Science ; Colorimetry ; Copper ; Ephedra ; Ephedra alata ; Ephedra altissima ; Flavonoids ; Gallic acid ; High performance liquid chromatography ; Ionization ; Ions ; Iron ; Liquid chromatography ; Magnesium ; Mass spectrometry ; Mass spectroscopy ; Original ; Original Article ; Phenolic acids ; Phenols ; potassium ; proanthocyanidins ; Scientific imaging ; Seeds ; Sodium ; Spectral analysis ; traditional medicine ; Tunisia ; zinc</subject><ispartof>Applied biochemistry and biotechnology, 2023-10, Vol.195 (10), p.5903-5915</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-9e7114aba73d1778b9adfdd7ad6e0f3d970db91b763bf2235ad9020b92f2a5d63</citedby><cites>FETCH-LOGICAL-c507t-9e7114aba73d1778b9adfdd7ad6e0f3d970db91b763bf2235ad9020b92f2a5d63</cites><orcidid>0000-0002-4114-2959</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-023-04370-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-023-04370-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36719522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mahmoudi, Maher</creatorcontrib><creatorcontrib>Boughalleb, Fayçal</creatorcontrib><creatorcontrib>Maaloul, Samah</creatorcontrib><creatorcontrib>Mabrouk, Mahmoud</creatorcontrib><creatorcontrib>Abdellaoui, Raoudha</creatorcontrib><title>Phytochemical Screening, Antioxidant Potential, and LC–ESI–MS Profiling of Ephedra alata and Ephedra altissima Seeds Naturally Growing in Tunisia</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Most
Ephedra
species are adapted to arid and desert conditions and are widely used in folk medicine to treat several disorders. The design of the current study was to determine the functional properties of seeds of two
Ephedra
species (
E. alata
and
E. altissima
) naturally growing in Tunisian arid zones by evaluating their mineral contents and bioactive compounds. The flame atomic absorption spectrometry revealed that seeds contained remarkable amounts of sodium (Na), potassium (K), calcium (Ca), magnesium (Mg), copper (Cu), zinc (Zn), and iron (Fe). The colorimetric investigation revealed high total polyphenol, flavonoid, and condensed tannin contents. Furthermore, by utilizing high-performance liquid chromatography-electrospray ionization–mass spectrometry method (HPLC-ESI/MS), a total of 11 phenolics were identified and quantified including 7 flavonoid compounds and 4 phenolic acids that were mostly predominated by gallic acid and quercetrin. Results so far have been very encouraging and proved that
Ephedra
seeds are a valuable source of natural bioactive compounds and minerals which could potentially be used for industrial and pharmaceutical purposes.</description><subject>antioxidant activity</subject><subject>Arid zones</subject><subject>Aridity</subject><subject>atomic absorption spectrometry</subject><subject>Atomic absorption spectroscopy</subject><subject>Bioactive compounds</subject><subject>Biochemistry</subject><subject>Biological activity</subject><subject>Biotechnology</subject><subject>Calcium</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Colorimetry</subject><subject>Copper</subject><subject>Ephedra</subject><subject>Ephedra alata</subject><subject>Ephedra altissima</subject><subject>Flavonoids</subject><subject>Gallic acid</subject><subject>High performance liquid chromatography</subject><subject>Ionization</subject><subject>Ions</subject><subject>Iron</subject><subject>Liquid chromatography</subject><subject>Magnesium</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Original</subject><subject>Original Article</subject><subject>Phenolic acids</subject><subject>Phenols</subject><subject>potassium</subject><subject>proanthocyanidins</subject><subject>Scientific imaging</subject><subject>Seeds</subject><subject>Sodium</subject><subject>Spectral analysis</subject><subject>traditional 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Screening, Antioxidant Potential, and LC–ESI–MS Profiling of Ephedra alata and Ephedra altissima Seeds Naturally Growing in Tunisia</title><author>Mahmoudi, Maher ; Boughalleb, Fayçal ; Maaloul, Samah ; Mabrouk, Mahmoud ; Abdellaoui, Raoudha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-9e7114aba73d1778b9adfdd7ad6e0f3d970db91b763bf2235ad9020b92f2a5d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>antioxidant activity</topic><topic>Arid zones</topic><topic>Aridity</topic><topic>atomic absorption spectrometry</topic><topic>Atomic absorption spectroscopy</topic><topic>Bioactive compounds</topic><topic>Biochemistry</topic><topic>Biological activity</topic><topic>Biotechnology</topic><topic>Calcium</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Colorimetry</topic><topic>Copper</topic><topic>Ephedra</topic><topic>Ephedra alata</topic><topic>Ephedra altissima</topic><topic>Flavonoids</topic><topic>Gallic acid</topic><topic>High performance liquid chromatography</topic><topic>Ionization</topic><topic>Ions</topic><topic>Iron</topic><topic>Liquid chromatography</topic><topic>Magnesium</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Original</topic><topic>Original Article</topic><topic>Phenolic acids</topic><topic>Phenols</topic><topic>potassium</topic><topic>proanthocyanidins</topic><topic>Scientific imaging</topic><topic>Seeds</topic><topic>Sodium</topic><topic>Spectral analysis</topic><topic>traditional medicine</topic><topic>Tunisia</topic><topic>zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mahmoudi, Maher</creatorcontrib><creatorcontrib>Boughalleb, Fayçal</creatorcontrib><creatorcontrib>Maaloul, Samah</creatorcontrib><creatorcontrib>Mabrouk, Mahmoud</creatorcontrib><creatorcontrib>Abdellaoui, 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Samah</au><au>Mabrouk, Mahmoud</au><au>Abdellaoui, Raoudha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phytochemical Screening, Antioxidant Potential, and LC–ESI–MS Profiling of Ephedra alata and Ephedra altissima Seeds Naturally Growing in Tunisia</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2023-10-01</date><risdate>2023</risdate><volume>195</volume><issue>10</issue><spage>5903</spage><epage>5915</epage><pages>5903-5915</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Most
Ephedra
species are adapted to arid and desert conditions and are widely used in folk medicine to treat several disorders. The design of the current study was to determine the functional properties of seeds of two
Ephedra
species (
E. alata
and
E. altissima
) naturally growing in Tunisian arid zones by evaluating their mineral contents and bioactive compounds. The flame atomic absorption spectrometry revealed that seeds contained remarkable amounts of sodium (Na), potassium (K), calcium (Ca), magnesium (Mg), copper (Cu), zinc (Zn), and iron (Fe). The colorimetric investigation revealed high total polyphenol, flavonoid, and condensed tannin contents. Furthermore, by utilizing high-performance liquid chromatography-electrospray ionization–mass spectrometry method (HPLC-ESI/MS), a total of 11 phenolics were identified and quantified including 7 flavonoid compounds and 4 phenolic acids that were mostly predominated by gallic acid and quercetrin. Results so far have been very encouraging and proved that
Ephedra
seeds are a valuable source of natural bioactive compounds and minerals which could potentially be used for industrial and pharmaceutical purposes.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>36719522</pmid><doi>10.1007/s12010-023-04370-8</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-4114-2959</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied biochemistry and biotechnology, 2023-10, Vol.195 (10), p.5903-5915 |
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| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9887572 |
| source | Springer Nature - Complete Springer Journals |
| subjects | antioxidant activity Arid zones Aridity atomic absorption spectrometry Atomic absorption spectroscopy Bioactive compounds Biochemistry Biological activity Biotechnology Calcium Chemistry Chemistry and Materials Science Colorimetry Copper Ephedra Ephedra alata Ephedra altissima Flavonoids Gallic acid High performance liquid chromatography Ionization Ions Iron Liquid chromatography Magnesium Mass spectrometry Mass spectroscopy Original Original Article Phenolic acids Phenols potassium proanthocyanidins Scientific imaging Seeds Sodium Spectral analysis traditional medicine Tunisia zinc |
| title | Phytochemical Screening, Antioxidant Potential, and LC–ESI–MS Profiling of Ephedra alata and Ephedra altissima Seeds Naturally Growing in Tunisia |
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