A Robust Method for Perfusable Microvascular Network Formation In Vitro
Micropost‐based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key chara...
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| Veröffentlicht in: | Small methods 2022-06, Vol.6 (6), p.e2200143-n/a |
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| creator | Wan, Zhengpeng Zhong, Amy X. Zhang, Shun Pavlou, Georgios Coughlin, Mark F. Shelton, Sarah E. Nguyen, Huu Tuan Lorch, Jochen H. Barbie, David A. Kamm, Roger D. |
| description | Micropost‐based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two‐step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established.
Microvascular network (MVN) generated in microfluidic devices are widely used in diverse research fields. But, forming perfusable MVNs with physiological diameters has proven to be challenging. In this study, seeding parameters that control MVN geometry are explored and a robust two‐step seeding method to form perfusable physiological MVNs with various vascular cells is established. |
| doi_str_mv | 10.1002/smtd.202200143 |
| format | Article |
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Microvascular network (MVN) generated in microfluidic devices are widely used in diverse research fields. But, forming perfusable MVNs with physiological diameters has proven to be challenging. In this study, seeding parameters that control MVN geometry are explored and a robust two‐step seeding method to form perfusable physiological MVNs with various vascular cells is established.</description><identifier>ISSN: 2366-9608</identifier><identifier>EISSN: 2366-9608</identifier><identifier>DOI: 10.1002/smtd.202200143</identifier><identifier>PMID: 35373502</identifier><language>eng</language><publisher>Germany</publisher><subject>cancer ; Lab-On-A-Chip Devices ; microfluidics ; microvasculatures ; Microvessels ; Neovascularization, Physiologic - physiology ; perfusable ; physiological geometry</subject><ispartof>Small methods, 2022-06, Vol.6 (6), p.e2200143-n/a</ispartof><rights>2022 The Authors. Small Methods published by Wiley‐VCH GmbH</rights><rights>2022 The Authors. Small Methods published by Wiley-VCH GmbH.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4403-2a581d1ae6cfc7715c6e4aa68690256457eaa8fcba6ecd201faee0f4ad646d63</citedby><cites>FETCH-LOGICAL-c4403-2a581d1ae6cfc7715c6e4aa68690256457eaa8fcba6ecd201faee0f4ad646d63</cites><orcidid>0000-0002-7232-304X ; 0000-0001-5626-7722</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fsmtd.202200143$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fsmtd.202200143$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,777,781,882,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35373502$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wan, Zhengpeng</creatorcontrib><creatorcontrib>Zhong, Amy X.</creatorcontrib><creatorcontrib>Zhang, Shun</creatorcontrib><creatorcontrib>Pavlou, Georgios</creatorcontrib><creatorcontrib>Coughlin, Mark F.</creatorcontrib><creatorcontrib>Shelton, Sarah E.</creatorcontrib><creatorcontrib>Nguyen, Huu Tuan</creatorcontrib><creatorcontrib>Lorch, Jochen H.</creatorcontrib><creatorcontrib>Barbie, David A.</creatorcontrib><creatorcontrib>Kamm, Roger D.</creatorcontrib><title>A Robust Method for Perfusable Microvascular Network Formation In Vitro</title><title>Small methods</title><addtitle>Small Methods</addtitle><description>Micropost‐based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two‐step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established.
Microvascular network (MVN) generated in microfluidic devices are widely used in diverse research fields. But, forming perfusable MVNs with physiological diameters has proven to be challenging. 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However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two‐step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established.
Microvascular network (MVN) generated in microfluidic devices are widely used in diverse research fields. But, forming perfusable MVNs with physiological diameters has proven to be challenging. In this study, seeding parameters that control MVN geometry are explored and a robust two‐step seeding method to form perfusable physiological MVNs with various vascular cells is established.</abstract><cop>Germany</cop><pmid>35373502</pmid><doi>10.1002/smtd.202200143</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-7232-304X</orcidid><orcidid>https://orcid.org/0000-0001-5626-7722</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | cancer Lab-On-A-Chip Devices microfluidics microvasculatures Microvessels Neovascularization, Physiologic - physiology perfusable physiological geometry |
| title | A Robust Method for Perfusable Microvascular Network Formation In Vitro |
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