Carbon Isotope Ratios of Plasma and RBC Fatty Acids Identify Meat Consumers in a 12-Week Inpatient Feeding Study of 32 Men
Molecular stable isotope ratios are a novel type of dietary biomarker with high sensitivity and specificity for certain foods. Among these, fatty acid carbon isotope ratios (CIRs) have strong potential but have not been investigated as dietary biomarkers. We evaluated whether fatty acid CIRs and mas...
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| Veröffentlicht in: | The Journal of nutrition 2022-12, Vol.152 (12), p.2847-2855 |
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| creator | Mitchell, Cassie M Oxtoby, Laura E Shaw, Pamela A Budge, Suzanne M Wooller, Matthew J Cabeza de Baca, Tomás Krakoff, Jonathan Votruba, Susanne O’Brien, Diane M |
| description | Molecular stable isotope ratios are a novel type of dietary biomarker with high sensitivity and specificity for certain foods. Among these, fatty acid carbon isotope ratios (CIRs) have strong potential but have not been investigated as dietary biomarkers.
We evaluated whether fatty acid CIRs and mass proportions were associated with meat, fish, and sugar-sweetened beverage (SSB) intake.
Thirty-two men [aged 46.2 ± 10.5 y; BMI (kg/m2): 27.2 ± 4.0] underwent a 12-wk inpatient dietary intervention at the National Institute of Diabetes and Digestive and Kidney Diseases in Phoenix, Arizona. Men were randomly assigned to 1 of 8 dietary treatments varying the presence/absence of dietary meat, fish, and SSBs in all combinations. Fatty acid CIRs and mass proportions were measured in fasting blood samples and adipose tissue biopsies that were collected pre- and postintervention. Dietary effects were analyzed using multivariable regression and receiver operating characteristic AUCs were calculated using logistic regression.
CIRs of the several abundant SFAs, MUFAs and arachidonic acid (20:4n-6) in plasma were strongly associated with meat, as were a subset of these fatty acids in RBCs. Effect sizes in plasma ranged from 1.01‰ to 1.93‰ and were similar but attenuated in RBCs. Mass proportions of those fatty acids were not associated with diet. CIRs of plasma dihomo-γ-linolenic acid (20:3n–6) and adipose palmitic acid (16:0) were weakly associated with SSBs. Mass proportions of plasma odd-chain fatty acids were associated with meat, and mass proportions of plasma EPA and DHA (20:5n–3 and 22:6n–3) were associated with fish.
CIRs of plasma and RBC fatty acids show promise as sensitive and specific measures of dietary meat. These provide different information from that provided by fatty acid mass proportions, and are informative where mass proportion is not. This trial is registered at www.clinicaltrials.gov as NCT01237093. |
| doi_str_mv | 10.1093/jn/nxac213 |
| format | Article |
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We evaluated whether fatty acid CIRs and mass proportions were associated with meat, fish, and sugar-sweetened beverage (SSB) intake.
Thirty-two men [aged 46.2 ± 10.5 y; BMI (kg/m2): 27.2 ± 4.0] underwent a 12-wk inpatient dietary intervention at the National Institute of Diabetes and Digestive and Kidney Diseases in Phoenix, Arizona. Men were randomly assigned to 1 of 8 dietary treatments varying the presence/absence of dietary meat, fish, and SSBs in all combinations. Fatty acid CIRs and mass proportions were measured in fasting blood samples and adipose tissue biopsies that were collected pre- and postintervention. Dietary effects were analyzed using multivariable regression and receiver operating characteristic AUCs were calculated using logistic regression.
CIRs of the several abundant SFAs, MUFAs and arachidonic acid (20:4n-6) in plasma were strongly associated with meat, as were a subset of these fatty acids in RBCs. Effect sizes in plasma ranged from 1.01‰ to 1.93‰ and were similar but attenuated in RBCs. Mass proportions of those fatty acids were not associated with diet. CIRs of plasma dihomo-γ-linolenic acid (20:3n–6) and adipose palmitic acid (16:0) were weakly associated with SSBs. Mass proportions of plasma odd-chain fatty acids were associated with meat, and mass proportions of plasma EPA and DHA (20:5n–3 and 22:6n–3) were associated with fish.
CIRs of plasma and RBC fatty acids show promise as sensitive and specific measures of dietary meat. These provide different information from that provided by fatty acid mass proportions, and are informative where mass proportion is not. This trial is registered at www.clinicaltrials.gov as NCT01237093.</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.1093/jn/nxac213</identifier><identifier>PMID: 36095134</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adipose tissue ; Animals ; Arachidonic acid ; Biomarkers ; Biopsy ; Carbon ; carbon isotope ratios ; Carbon Isotopes ; controlled-feeding study ; Diabetes mellitus ; Diet ; dietary biomarkers ; Fatty Acids ; Fish ; g-linolenic acid ; Humans ; Inpatients ; Isotope ratios ; Kidney diseases ; Linolenic acid ; Meat ; molecular stable isotope ratios ; Nutrition ; Nutritional Epidemiology ; Palmitic acid ; Stable isotopes</subject><ispartof>The Journal of nutrition, 2022-12, Vol.152 (12), p.2847-2855</ispartof><rights>2022 American Society for Nutrition.</rights><rights>The Author(s) 2022. Published by Oxford University Press on behalf of the American Society for Nutrition.</rights><rights>Copyright American Institute of Nutrition Dec 2022</rights><rights>The Author(s) 2022. Published by Oxford University Press on behalf of the American Society for Nutrition. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3253-ab789c050905c31303c42e9591c478513fed540de84774e5fa96f341d183db153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36095134$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitchell, Cassie M</creatorcontrib><creatorcontrib>Oxtoby, Laura E</creatorcontrib><creatorcontrib>Shaw, Pamela A</creatorcontrib><creatorcontrib>Budge, Suzanne M</creatorcontrib><creatorcontrib>Wooller, Matthew J</creatorcontrib><creatorcontrib>Cabeza de Baca, Tomás</creatorcontrib><creatorcontrib>Krakoff, Jonathan</creatorcontrib><creatorcontrib>Votruba, Susanne</creatorcontrib><creatorcontrib>O’Brien, Diane M</creatorcontrib><title>Carbon Isotope Ratios of Plasma and RBC Fatty Acids Identify Meat Consumers in a 12-Week Inpatient Feeding Study of 32 Men</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>Molecular stable isotope ratios are a novel type of dietary biomarker with high sensitivity and specificity for certain foods. Among these, fatty acid carbon isotope ratios (CIRs) have strong potential but have not been investigated as dietary biomarkers.
We evaluated whether fatty acid CIRs and mass proportions were associated with meat, fish, and sugar-sweetened beverage (SSB) intake.
Thirty-two men [aged 46.2 ± 10.5 y; BMI (kg/m2): 27.2 ± 4.0] underwent a 12-wk inpatient dietary intervention at the National Institute of Diabetes and Digestive and Kidney Diseases in Phoenix, Arizona. Men were randomly assigned to 1 of 8 dietary treatments varying the presence/absence of dietary meat, fish, and SSBs in all combinations. Fatty acid CIRs and mass proportions were measured in fasting blood samples and adipose tissue biopsies that were collected pre- and postintervention. Dietary effects were analyzed using multivariable regression and receiver operating characteristic AUCs were calculated using logistic regression.
CIRs of the several abundant SFAs, MUFAs and arachidonic acid (20:4n-6) in plasma were strongly associated with meat, as were a subset of these fatty acids in RBCs. Effect sizes in plasma ranged from 1.01‰ to 1.93‰ and were similar but attenuated in RBCs. Mass proportions of those fatty acids were not associated with diet. CIRs of plasma dihomo-γ-linolenic acid (20:3n–6) and adipose palmitic acid (16:0) were weakly associated with SSBs. Mass proportions of plasma odd-chain fatty acids were associated with meat, and mass proportions of plasma EPA and DHA (20:5n–3 and 22:6n–3) were associated with fish.
CIRs of plasma and RBC fatty acids show promise as sensitive and specific measures of dietary meat. These provide different information from that provided by fatty acid mass proportions, and are informative where mass proportion is not. This trial is registered at www.clinicaltrials.gov as NCT01237093.</description><subject>Adipose tissue</subject><subject>Animals</subject><subject>Arachidonic acid</subject><subject>Biomarkers</subject><subject>Biopsy</subject><subject>Carbon</subject><subject>carbon isotope ratios</subject><subject>Carbon Isotopes</subject><subject>controlled-feeding study</subject><subject>Diabetes mellitus</subject><subject>Diet</subject><subject>dietary biomarkers</subject><subject>Fatty Acids</subject><subject>Fish</subject><subject>g-linolenic acid</subject><subject>Humans</subject><subject>Inpatients</subject><subject>Isotope ratios</subject><subject>Kidney diseases</subject><subject>Linolenic acid</subject><subject>Meat</subject><subject>molecular stable isotope ratios</subject><subject>Nutrition</subject><subject>Nutritional Epidemiology</subject><subject>Palmitic acid</subject><subject>Stable isotopes</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc1uEzEURi1ERUNhwwMgS-wqDfXvzHiDVEYNRGoFKiCWlmPfKQ6JHWxPRXh6HCVUILHywsfH934fQi8oeU2J4hercBF-Gssof4RmVAratJSQx2hGCGMNp217ip7mvCKEUKH6J-iUt0RJysUM_RpMWsaAFzmWuAV8a4qPGccRf1ybvDHYBIdv3w54bkrZ4UvrXcYLB6H4cYdvwBQ8xJCnDaSMfcAGU9Z8BfiOF2FbXRXEcwDnwx3-VCa326s5qy_DM3QymnWG58fzDH2ZX30e3jfXH94thsvrxnImeWOWXa8skUQRaTnlhFvBQElFrej6usUITgrioBddJ0CORrUjF9TRnrsllfwMvTl4t9NyA87WkZJZ623yG5N2Ohqv_70J_pu-i_da9VwptRe8OgpS_DFBLnoVpxTqzJp1bcckIYpW6vxA2RRzTjA-_ECJ3vekV0Efe6rwy79nekD_FFMBcQCgJnPvIelsa5i2JpnAFu2i_5_3N7Ayn9w</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Mitchell, Cassie M</creator><creator>Oxtoby, Laura E</creator><creator>Shaw, Pamela A</creator><creator>Budge, Suzanne M</creator><creator>Wooller, Matthew J</creator><creator>Cabeza de Baca, Tomás</creator><creator>Krakoff, Jonathan</creator><creator>Votruba, Susanne</creator><creator>O’Brien, Diane M</creator><general>Elsevier Inc</general><general>American Institute of Nutrition</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>5PM</scope></search><sort><creationdate>20221201</creationdate><title>Carbon Isotope Ratios of Plasma and RBC Fatty Acids Identify Meat Consumers in a 12-Week Inpatient Feeding Study of 32 Men</title><author>Mitchell, Cassie M ; Oxtoby, Laura E ; Shaw, Pamela A ; Budge, Suzanne M ; Wooller, Matthew J ; Cabeza de Baca, Tomás ; Krakoff, Jonathan ; Votruba, Susanne ; O’Brien, Diane M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3253-ab789c050905c31303c42e9591c478513fed540de84774e5fa96f341d183db153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adipose tissue</topic><topic>Animals</topic><topic>Arachidonic acid</topic><topic>Biomarkers</topic><topic>Biopsy</topic><topic>Carbon</topic><topic>carbon isotope ratios</topic><topic>Carbon Isotopes</topic><topic>controlled-feeding study</topic><topic>Diabetes mellitus</topic><topic>Diet</topic><topic>dietary biomarkers</topic><topic>Fatty Acids</topic><topic>Fish</topic><topic>g-linolenic acid</topic><topic>Humans</topic><topic>Inpatients</topic><topic>Isotope ratios</topic><topic>Kidney diseases</topic><topic>Linolenic acid</topic><topic>Meat</topic><topic>molecular stable isotope ratios</topic><topic>Nutrition</topic><topic>Nutritional Epidemiology</topic><topic>Palmitic acid</topic><topic>Stable isotopes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitchell, Cassie M</creatorcontrib><creatorcontrib>Oxtoby, Laura E</creatorcontrib><creatorcontrib>Shaw, Pamela A</creatorcontrib><creatorcontrib>Budge, Suzanne M</creatorcontrib><creatorcontrib>Wooller, Matthew J</creatorcontrib><creatorcontrib>Cabeza de Baca, Tomás</creatorcontrib><creatorcontrib>Krakoff, Jonathan</creatorcontrib><creatorcontrib>Votruba, Susanne</creatorcontrib><creatorcontrib>O’Brien, Diane M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitchell, Cassie M</au><au>Oxtoby, Laura E</au><au>Shaw, Pamela A</au><au>Budge, Suzanne M</au><au>Wooller, Matthew J</au><au>Cabeza de Baca, Tomás</au><au>Krakoff, Jonathan</au><au>Votruba, Susanne</au><au>O’Brien, Diane M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carbon Isotope Ratios of Plasma and RBC Fatty Acids Identify Meat Consumers in a 12-Week Inpatient Feeding Study of 32 Men</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>2022-12-01</date><risdate>2022</risdate><volume>152</volume><issue>12</issue><spage>2847</spage><epage>2855</epage><pages>2847-2855</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><abstract>Molecular stable isotope ratios are a novel type of dietary biomarker with high sensitivity and specificity for certain foods. Among these, fatty acid carbon isotope ratios (CIRs) have strong potential but have not been investigated as dietary biomarkers.
We evaluated whether fatty acid CIRs and mass proportions were associated with meat, fish, and sugar-sweetened beverage (SSB) intake.
Thirty-two men [aged 46.2 ± 10.5 y; BMI (kg/m2): 27.2 ± 4.0] underwent a 12-wk inpatient dietary intervention at the National Institute of Diabetes and Digestive and Kidney Diseases in Phoenix, Arizona. Men were randomly assigned to 1 of 8 dietary treatments varying the presence/absence of dietary meat, fish, and SSBs in all combinations. Fatty acid CIRs and mass proportions were measured in fasting blood samples and adipose tissue biopsies that were collected pre- and postintervention. Dietary effects were analyzed using multivariable regression and receiver operating characteristic AUCs were calculated using logistic regression.
CIRs of the several abundant SFAs, MUFAs and arachidonic acid (20:4n-6) in plasma were strongly associated with meat, as were a subset of these fatty acids in RBCs. Effect sizes in plasma ranged from 1.01‰ to 1.93‰ and were similar but attenuated in RBCs. Mass proportions of those fatty acids were not associated with diet. CIRs of plasma dihomo-γ-linolenic acid (20:3n–6) and adipose palmitic acid (16:0) were weakly associated with SSBs. Mass proportions of plasma odd-chain fatty acids were associated with meat, and mass proportions of plasma EPA and DHA (20:5n–3 and 22:6n–3) were associated with fish.
CIRs of plasma and RBC fatty acids show promise as sensitive and specific measures of dietary meat. These provide different information from that provided by fatty acid mass proportions, and are informative where mass proportion is not. This trial is registered at www.clinicaltrials.gov as NCT01237093.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>36095134</pmid><doi>10.1093/jn/nxac213</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adipose tissue Animals Arachidonic acid Biomarkers Biopsy Carbon carbon isotope ratios Carbon Isotopes controlled-feeding study Diabetes mellitus Diet dietary biomarkers Fatty Acids Fish g-linolenic acid Humans Inpatients Isotope ratios Kidney diseases Linolenic acid Meat molecular stable isotope ratios Nutrition Nutritional Epidemiology Palmitic acid Stable isotopes |
| title | Carbon Isotope Ratios of Plasma and RBC Fatty Acids Identify Meat Consumers in a 12-Week Inpatient Feeding Study of 32 Men |
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