An efficient and cost-effective method for directed mutagenesis at multiple dispersed sites—a case study with Omicron Spike DNA

Abstract Site-directed mutagenesis is an invaluable technique that enables the elucidation of the contribution of specific residues to protein structure and function. The simultaneous introduction of mutations at a large number of sites (>10), singly and in multiple combinations, is often necessa...

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Veröffentlicht in:	Biology methods and protocols 2023, Vol.8 (1), p.bpac037-bpac037
Hauptverfasser:	Rani, Rita, Parsa, Kishore V L, Chatti, Kiranam, Sevilimedu, Aarti
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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