Honeycomb-like Structured Film, a Novel Therapeutic Device, Suppresses Tumor Growth in an In Vivo Ovarian Cancer Model
Ovarian cancer cell dissemination can lead to the mortality of patients with advanced ovarian cancer. Complete surgery for no gross residual disease contributes to a more favorable prognosis than that of patients with residual disease. HCFs have highly regular porous structures and their 3D porous s...
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| Veröffentlicht in: | Cancers 2022-12, Vol.15 (1), p.237 |
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| creator | Ohta, Tsuyoshi Tanaka, Masaru Taki, Seitaro Nakagawa, Hiroyuki Nagase, Satoru |
| description | Ovarian cancer cell dissemination can lead to the mortality of patients with advanced ovarian cancer. Complete surgery for no gross residual disease contributes to a more favorable prognosis than that of patients with residual disease. HCFs have highly regular porous structures and their 3D porous structures act as scaffolds for cell adhesion. HCFs are fabricated from biodegradable polymers and have been widely used in tissue engineering. This study aimed to show that HCFs suppress tumor growth in an in vivo ovarian cancer model. The HCF pore sizes had a significant influence on tumor growth inhibition, and HCFs induced morphological changes that rounded out ovarian cancer cells. Furthermore, we identified gene ontology (GO) terms and clusters of genes downregulated by HCFs. qPCR analysis demonstrated that a honeycomb structure downregulated the expression of CXCL2, FOXC1, MMP14, and SNAI2, which are involved in cell proliferation, migration, invasion, angiogenesis, focal adhesion, extracellular matrix (ECM), and epithelial-mesenchymal transition (EMT). Collectively, HCFs induced abnormal focal adhesion and cell morphological changes, subsequently inhibiting the differentiation, proliferation and motility of ovarian cancer cells. Our data suggest that HCFs could be a novel device for inhibiting residual tumor growth after surgery, and could reduce surgical invasiveness and improve the prognosis for patients with advanced ovarian cancer. |
| doi_str_mv | 10.3390/cancers15010237 |
| format | Article |
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Complete surgery for no gross residual disease contributes to a more favorable prognosis than that of patients with residual disease. HCFs have highly regular porous structures and their 3D porous structures act as scaffolds for cell adhesion. HCFs are fabricated from biodegradable polymers and have been widely used in tissue engineering. This study aimed to show that HCFs suppress tumor growth in an in vivo ovarian cancer model. The HCF pore sizes had a significant influence on tumor growth inhibition, and HCFs induced morphological changes that rounded out ovarian cancer cells. Furthermore, we identified gene ontology (GO) terms and clusters of genes downregulated by HCFs. qPCR analysis demonstrated that a honeycomb structure downregulated the expression of CXCL2, FOXC1, MMP14, and SNAI2, which are involved in cell proliferation, migration, invasion, angiogenesis, focal adhesion, extracellular matrix (ECM), and epithelial-mesenchymal transition (EMT). Collectively, HCFs induced abnormal focal adhesion and cell morphological changes, subsequently inhibiting the differentiation, proliferation and motility of ovarian cancer cells. Our data suggest that HCFs could be a novel device for inhibiting residual tumor growth after surgery, and could reduce surgical invasiveness and improve the prognosis for patients with advanced ovarian cancer.</description><identifier>ISSN: 2072-6694</identifier><identifier>EISSN: 2072-6694</identifier><identifier>DOI: 10.3390/cancers15010237</identifier><identifier>PMID: 36612230</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Angiogenesis ; Biodegradability ; Cancer therapies ; Care and treatment ; Cell adhesion ; Cell differentiation ; Cell growth ; Cell migration ; Cell proliferation ; Extracellular matrix ; Gene expression ; Genetic engineering ; Genomes ; Invasiveness ; Medical prognosis ; Mesenchyme ; Ovarian cancer ; Pore size ; Prognosis ; Snail protein ; Surgery ; Tissue engineering ; Tumors</subject><ispartof>Cancers, 2022-12, Vol.15 (1), p.237</ispartof><rights>COPYRIGHT 2022 MDPI AG</rights><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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Complete surgery for no gross residual disease contributes to a more favorable prognosis than that of patients with residual disease. HCFs have highly regular porous structures and their 3D porous structures act as scaffolds for cell adhesion. HCFs are fabricated from biodegradable polymers and have been widely used in tissue engineering. This study aimed to show that HCFs suppress tumor growth in an in vivo ovarian cancer model. The HCF pore sizes had a significant influence on tumor growth inhibition, and HCFs induced morphological changes that rounded out ovarian cancer cells. Furthermore, we identified gene ontology (GO) terms and clusters of genes downregulated by HCFs. qPCR analysis demonstrated that a honeycomb structure downregulated the expression of CXCL2, FOXC1, MMP14, and SNAI2, which are involved in cell proliferation, migration, invasion, angiogenesis, focal adhesion, extracellular matrix (ECM), and epithelial-mesenchymal transition (EMT). 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Our data suggest that HCFs could be a novel device for inhibiting residual tumor growth after surgery, and could reduce surgical invasiveness and improve the prognosis for patients with advanced ovarian cancer.</description><subject>Angiogenesis</subject><subject>Biodegradability</subject><subject>Cancer therapies</subject><subject>Care and treatment</subject><subject>Cell adhesion</subject><subject>Cell differentiation</subject><subject>Cell growth</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Extracellular matrix</subject><subject>Gene expression</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Invasiveness</subject><subject>Medical prognosis</subject><subject>Mesenchyme</subject><subject>Ovarian cancer</subject><subject>Pore size</subject><subject>Prognosis</subject><subject>Snail protein</subject><subject>Surgery</subject><subject>Tissue engineering</subject><subject>Tumors</subject><issn>2072-6694</issn><issn>2072-6694</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptkk1P3DAQhqOqVUGUc2-VpV56IOBvJ5dKaFs-JFoObHu1HO-ENU3sYCep-Pf1LpQC6vjgseeZ1x57iuI9wYeM1fjIGm8hJiIwwZSpV8UuxYqWUtb89RN_p9hP6QZnY4woqd4WO0xKQinDu8V8Fjzc2dA3Zed-Aboa42THKcIKnbiuP0AGfQ8zdGi5hmgGmEZn0ReYnYUDdDUNQ4SUIKHl1IeITmP4Pa6R88h4dO7RTzcHdDmb6PJ6sb0u-hZW0L0r3rSmS7D_MO8VP06-Lhdn5cXl6fni-KK0nNOxlI0wrVJtg4GxxhBMhARBuKga01pag6CCN9wQIqlRBFemFbWiNIc5FhVje8Xne91hanpYWfBjNJ0eoutNvNPBOP084t1aX4dZ1xWpBN8IfHoQiOF2gjTq3iULXWc8hClpqiSpleJcZvTjC_QmTNHn8rYUrqts_6hr04F2vg35XLsR1ceKC6oY31KH_6HyWEHvbP6y1uX9ZwlH9wk2hpQitI81Eqw33aJfdEvO-PD0aR75v73B_gBKlLp4</recordid><startdate>20221230</startdate><enddate>20221230</enddate><creator>Ohta, Tsuyoshi</creator><creator>Tanaka, Masaru</creator><creator>Taki, Seitaro</creator><creator>Nakagawa, Hiroyuki</creator><creator>Nagase, Satoru</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1115-2080</orcidid><orcidid>https://orcid.org/0000-0002-4819-4233</orcidid><orcidid>https://orcid.org/0000-0001-5212-1128</orcidid></search><sort><creationdate>20221230</creationdate><title>Honeycomb-like Structured Film, a Novel Therapeutic Device, Suppresses Tumor Growth in an In Vivo Ovarian Cancer Model</title><author>Ohta, Tsuyoshi ; Tanaka, Masaru ; Taki, Seitaro ; Nakagawa, Hiroyuki ; Nagase, Satoru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-6b5af77fb0e33ba10156e51458bafc29e5254b4a1162a7108af597228ba405833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Angiogenesis</topic><topic>Biodegradability</topic><topic>Cancer therapies</topic><topic>Care and treatment</topic><topic>Cell adhesion</topic><topic>Cell differentiation</topic><topic>Cell growth</topic><topic>Cell migration</topic><topic>Cell proliferation</topic><topic>Extracellular matrix</topic><topic>Gene expression</topic><topic>Genetic engineering</topic><topic>Genomes</topic><topic>Invasiveness</topic><topic>Medical prognosis</topic><topic>Mesenchyme</topic><topic>Ovarian cancer</topic><topic>Pore size</topic><topic>Prognosis</topic><topic>Snail protein</topic><topic>Surgery</topic><topic>Tissue engineering</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohta, Tsuyoshi</creatorcontrib><creatorcontrib>Tanaka, Masaru</creatorcontrib><creatorcontrib>Taki, Seitaro</creatorcontrib><creatorcontrib>Nakagawa, Hiroyuki</creatorcontrib><creatorcontrib>Nagase, Satoru</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohta, Tsuyoshi</au><au>Tanaka, Masaru</au><au>Taki, Seitaro</au><au>Nakagawa, Hiroyuki</au><au>Nagase, Satoru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Honeycomb-like Structured Film, a Novel Therapeutic Device, Suppresses Tumor Growth in an In Vivo Ovarian Cancer Model</atitle><jtitle>Cancers</jtitle><addtitle>Cancers (Basel)</addtitle><date>2022-12-30</date><risdate>2022</risdate><volume>15</volume><issue>1</issue><spage>237</spage><pages>237-</pages><issn>2072-6694</issn><eissn>2072-6694</eissn><abstract>Ovarian cancer cell dissemination can lead to the mortality of patients with advanced ovarian cancer. Complete surgery for no gross residual disease contributes to a more favorable prognosis than that of patients with residual disease. HCFs have highly regular porous structures and their 3D porous structures act as scaffolds for cell adhesion. HCFs are fabricated from biodegradable polymers and have been widely used in tissue engineering. This study aimed to show that HCFs suppress tumor growth in an in vivo ovarian cancer model. The HCF pore sizes had a significant influence on tumor growth inhibition, and HCFs induced morphological changes that rounded out ovarian cancer cells. Furthermore, we identified gene ontology (GO) terms and clusters of genes downregulated by HCFs. qPCR analysis demonstrated that a honeycomb structure downregulated the expression of CXCL2, FOXC1, MMP14, and SNAI2, which are involved in cell proliferation, migration, invasion, angiogenesis, focal adhesion, extracellular matrix (ECM), and epithelial-mesenchymal transition (EMT). Collectively, HCFs induced abnormal focal adhesion and cell morphological changes, subsequently inhibiting the differentiation, proliferation and motility of ovarian cancer cells. Our data suggest that HCFs could be a novel device for inhibiting residual tumor growth after surgery, and could reduce surgical invasiveness and improve the prognosis for patients with advanced ovarian cancer.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>36612230</pmid><doi>10.3390/cancers15010237</doi><orcidid>https://orcid.org/0000-0002-1115-2080</orcidid><orcidid>https://orcid.org/0000-0002-4819-4233</orcidid><orcidid>https://orcid.org/0000-0001-5212-1128</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Angiogenesis Biodegradability Cancer therapies Care and treatment Cell adhesion Cell differentiation Cell growth Cell migration Cell proliferation Extracellular matrix Gene expression Genetic engineering Genomes Invasiveness Medical prognosis Mesenchyme Ovarian cancer Pore size Prognosis Snail protein Surgery Tissue engineering Tumors |
| title | Honeycomb-like Structured Film, a Novel Therapeutic Device, Suppresses Tumor Growth in an In Vivo Ovarian Cancer Model |
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