Biomechanical Microenvironment Regulates Fusogenicity of Breast Cancer Cells

Fusion of cancer cells is thought to contribute to tumor development and drug resistance. The low frequency of cell fusion events and the instability of fused cells have hindered our ability to understand the molecular mechanisms that govern cell fusion. We have demonstrated that several breast canc...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:ACS biomaterials science & engineering 2019-08, Vol.5 (8), p.3817-3827
Hauptverfasser: Zhu, Peiran, Tseng, Ning-Hsuan, Xie, Tianfa, Li, Ningwei, Fitts-Sprague, Isaac, Peyton, Shelly R, Sun, Yubing
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 3827
container_issue 8
container_start_page 3817
container_title ACS biomaterials science & engineering
container_volume 5
creator Zhu, Peiran
Tseng, Ning-Hsuan
Xie, Tianfa
Li, Ningwei
Fitts-Sprague, Isaac
Peyton, Shelly R
Sun, Yubing
description Fusion of cancer cells is thought to contribute to tumor development and drug resistance. The low frequency of cell fusion events and the instability of fused cells have hindered our ability to understand the molecular mechanisms that govern cell fusion. We have demonstrated that several breast cancer cell lines can fuse into multinucleated giant cells in vitro, and the initiation and longevity of fused cells can be regulated solely by biophysical factors. Dynamically tuning the adhesive area of the patterned substrates, reducing cytoskeletal tensions pharmacologically, altering matrix stiffness, and modulating pattern curvature all supported the spontaneous fusion and stability of these multinucleated giant cells. These observations highlight that the biomechanical microenvironment of cancer cells, including the matrix rigidity and interfacial curvature, can directly modulate their fusogenicity, an unexplored mechanism through which biophysical cues regulate tumor progression.
doi_str_mv 10.1021/acsbiomaterials.8b00861
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9800072</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2477512486</sourcerecordid><originalsourceid>FETCH-LOGICAL-a461t-57a29334add432fda15844552f0878dc69e6984b0919951defebb80e16d418783</originalsourceid><addsrcrecordid>eNqFkV9LwzAUxYMobsx9Be2jL5351zZ9EVxxKkwE0eeQtrdbRtvMpB3s25uxOaYvPiWQ3zn35FyEbgieEEzJnSpcrk2jOrBa1W4icoxFTM7QkLKEhalIxPnJfYDGzq0wxoSJiHN-iQaMcSY4pUM0n3onKJaq1YWqg1ddWAPtRlvTNtB2wTss-tpPcsGsd2YBHtPdNjBVMLWgXBdkqi3ABhnUtbtCF5UPBOPDOUKfs8eP7Dmcvz29ZA_zUPGYdGGUKJr6CKosOaNVqUgkOI8iWmEftyziFOJU8BynJE0jUkIFeS4wkLjkxBNshO73vus-b6AsfFCrarm2ulF2K43S8vdLq5dyYTYyFb6GhHqD24OBNV89uE422hX-C6oF0ztJeZJEhHIRezTZo74Y5yxUxzEEy9065J91yMM6vPL6NOVR91O-B9ge8A5yZXrb7uT_2X4DiRmeOQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2477512486</pqid></control><display><type>article</type><title>Biomechanical Microenvironment Regulates Fusogenicity of Breast Cancer Cells</title><source>ACS Publications</source><creator>Zhu, Peiran ; Tseng, Ning-Hsuan ; Xie, Tianfa ; Li, Ningwei ; Fitts-Sprague, Isaac ; Peyton, Shelly R ; Sun, Yubing</creator><creatorcontrib>Zhu, Peiran ; Tseng, Ning-Hsuan ; Xie, Tianfa ; Li, Ningwei ; Fitts-Sprague, Isaac ; Peyton, Shelly R ; Sun, Yubing</creatorcontrib><description>Fusion of cancer cells is thought to contribute to tumor development and drug resistance. The low frequency of cell fusion events and the instability of fused cells have hindered our ability to understand the molecular mechanisms that govern cell fusion. We have demonstrated that several breast cancer cell lines can fuse into multinucleated giant cells in vitro, and the initiation and longevity of fused cells can be regulated solely by biophysical factors. Dynamically tuning the adhesive area of the patterned substrates, reducing cytoskeletal tensions pharmacologically, altering matrix stiffness, and modulating pattern curvature all supported the spontaneous fusion and stability of these multinucleated giant cells. These observations highlight that the biomechanical microenvironment of cancer cells, including the matrix rigidity and interfacial curvature, can directly modulate their fusogenicity, an unexplored mechanism through which biophysical cues regulate tumor progression.</description><identifier>ISSN: 2373-9878</identifier><identifier>EISSN: 2373-9878</identifier><identifier>DOI: 10.1021/acsbiomaterials.8b00861</identifier><identifier>PMID: 33438422</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><ispartof>ACS biomaterials science &amp; engineering, 2019-08, Vol.5 (8), p.3817-3827</ispartof><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a461t-57a29334add432fda15844552f0878dc69e6984b0919951defebb80e16d418783</citedby><cites>FETCH-LOGICAL-a461t-57a29334add432fda15844552f0878dc69e6984b0919951defebb80e16d418783</cites><orcidid>0000-0001-6007-6187 ; 0000-0002-6831-3383</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acsbiomaterials.8b00861$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acsbiomaterials.8b00861$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,315,781,785,886,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33438422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Peiran</creatorcontrib><creatorcontrib>Tseng, Ning-Hsuan</creatorcontrib><creatorcontrib>Xie, Tianfa</creatorcontrib><creatorcontrib>Li, Ningwei</creatorcontrib><creatorcontrib>Fitts-Sprague, Isaac</creatorcontrib><creatorcontrib>Peyton, Shelly R</creatorcontrib><creatorcontrib>Sun, Yubing</creatorcontrib><title>Biomechanical Microenvironment Regulates Fusogenicity of Breast Cancer Cells</title><title>ACS biomaterials science &amp; engineering</title><addtitle>ACS Biomater. Sci. Eng</addtitle><description>Fusion of cancer cells is thought to contribute to tumor development and drug resistance. The low frequency of cell fusion events and the instability of fused cells have hindered our ability to understand the molecular mechanisms that govern cell fusion. We have demonstrated that several breast cancer cell lines can fuse into multinucleated giant cells in vitro, and the initiation and longevity of fused cells can be regulated solely by biophysical factors. Dynamically tuning the adhesive area of the patterned substrates, reducing cytoskeletal tensions pharmacologically, altering matrix stiffness, and modulating pattern curvature all supported the spontaneous fusion and stability of these multinucleated giant cells. These observations highlight that the biomechanical microenvironment of cancer cells, including the matrix rigidity and interfacial curvature, can directly modulate their fusogenicity, an unexplored mechanism through which biophysical cues regulate tumor progression.</description><issn>2373-9878</issn><issn>2373-9878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFkV9LwzAUxYMobsx9Be2jL5351zZ9EVxxKkwE0eeQtrdbRtvMpB3s25uxOaYvPiWQ3zn35FyEbgieEEzJnSpcrk2jOrBa1W4icoxFTM7QkLKEhalIxPnJfYDGzq0wxoSJiHN-iQaMcSY4pUM0n3onKJaq1YWqg1ddWAPtRlvTNtB2wTss-tpPcsGsd2YBHtPdNjBVMLWgXBdkqi3ABhnUtbtCF5UPBOPDOUKfs8eP7Dmcvz29ZA_zUPGYdGGUKJr6CKosOaNVqUgkOI8iWmEftyziFOJU8BynJE0jUkIFeS4wkLjkxBNshO73vus-b6AsfFCrarm2ulF2K43S8vdLq5dyYTYyFb6GhHqD24OBNV89uE422hX-C6oF0ztJeZJEhHIRezTZo74Y5yxUxzEEy9065J91yMM6vPL6NOVR91O-B9ge8A5yZXrb7uT_2X4DiRmeOQ</recordid><startdate>20190812</startdate><enddate>20190812</enddate><creator>Zhu, Peiran</creator><creator>Tseng, Ning-Hsuan</creator><creator>Xie, Tianfa</creator><creator>Li, Ningwei</creator><creator>Fitts-Sprague, Isaac</creator><creator>Peyton, Shelly R</creator><creator>Sun, Yubing</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6007-6187</orcidid><orcidid>https://orcid.org/0000-0002-6831-3383</orcidid></search><sort><creationdate>20190812</creationdate><title>Biomechanical Microenvironment Regulates Fusogenicity of Breast Cancer Cells</title><author>Zhu, Peiran ; Tseng, Ning-Hsuan ; Xie, Tianfa ; Li, Ningwei ; Fitts-Sprague, Isaac ; Peyton, Shelly R ; Sun, Yubing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a461t-57a29334add432fda15844552f0878dc69e6984b0919951defebb80e16d418783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Peiran</creatorcontrib><creatorcontrib>Tseng, Ning-Hsuan</creatorcontrib><creatorcontrib>Xie, Tianfa</creatorcontrib><creatorcontrib>Li, Ningwei</creatorcontrib><creatorcontrib>Fitts-Sprague, Isaac</creatorcontrib><creatorcontrib>Peyton, Shelly R</creatorcontrib><creatorcontrib>Sun, Yubing</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>ACS biomaterials science &amp; engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Peiran</au><au>Tseng, Ning-Hsuan</au><au>Xie, Tianfa</au><au>Li, Ningwei</au><au>Fitts-Sprague, Isaac</au><au>Peyton, Shelly R</au><au>Sun, Yubing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biomechanical Microenvironment Regulates Fusogenicity of Breast Cancer Cells</atitle><jtitle>ACS biomaterials science &amp; engineering</jtitle><addtitle>ACS Biomater. Sci. Eng</addtitle><date>2019-08-12</date><risdate>2019</risdate><volume>5</volume><issue>8</issue><spage>3817</spage><epage>3827</epage><pages>3817-3827</pages><issn>2373-9878</issn><eissn>2373-9878</eissn><abstract>Fusion of cancer cells is thought to contribute to tumor development and drug resistance. The low frequency of cell fusion events and the instability of fused cells have hindered our ability to understand the molecular mechanisms that govern cell fusion. We have demonstrated that several breast cancer cell lines can fuse into multinucleated giant cells in vitro, and the initiation and longevity of fused cells can be regulated solely by biophysical factors. Dynamically tuning the adhesive area of the patterned substrates, reducing cytoskeletal tensions pharmacologically, altering matrix stiffness, and modulating pattern curvature all supported the spontaneous fusion and stability of these multinucleated giant cells. These observations highlight that the biomechanical microenvironment of cancer cells, including the matrix rigidity and interfacial curvature, can directly modulate their fusogenicity, an unexplored mechanism through which biophysical cues regulate tumor progression.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>33438422</pmid><doi>10.1021/acsbiomaterials.8b00861</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-6007-6187</orcidid><orcidid>https://orcid.org/0000-0002-6831-3383</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2373-9878
ispartof ACS biomaterials science & engineering, 2019-08, Vol.5 (8), p.3817-3827
issn 2373-9878
2373-9878
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9800072
source ACS Publications
title Biomechanical Microenvironment Regulates Fusogenicity of Breast Cancer Cells
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T07%3A41%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biomechanical%20Microenvironment%20Regulates%20Fusogenicity%20of%20Breast%20Cancer%20Cells&rft.jtitle=ACS%20biomaterials%20science%20&%20engineering&rft.au=Zhu,%20Peiran&rft.date=2019-08-12&rft.volume=5&rft.issue=8&rft.spage=3817&rft.epage=3827&rft.pages=3817-3827&rft.issn=2373-9878&rft.eissn=2373-9878&rft_id=info:doi/10.1021/acsbiomaterials.8b00861&rft_dat=%3Cproquest_pubme%3E2477512486%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2477512486&rft_id=info:pmid/33438422&rfr_iscdi=true