A Tet-Inducible CRISPR Platform for High-Fidelity Editing of Human Pluripotent Stem Cells

Pluripotent stem cells (PSCs) offer an exciting resource for probing human biology; however, gene-editing efficiency remains relatively low in many cell types, including stem cells. Gene-editing using the CRISPR-Cas9 system offers an attractive solution that improves upon previous gene-editing appro...

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Veröffentlicht in:	Genes 2022-12, Vol.13 (12), p.2363
Hauptverfasser:	Jurlina, Shawna L, Jones, Melissa K, Agarwal, Devansh, De La Toba, Diana V, Kambli, Netra, Su, Fei, Martin, Heather M, Anderson, Ryan, Wong, Ryan M, Seid, Justin, Attaluri, Saisantosh V, Chow, Melissa, Wahlin, Karl J
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell lines Cloning CRISPR CRISPR-Cas Systems - genetics DNA End-Joining Repair DNA methylation E coli Efficiency Enzymes Fidelity Gene Editing - methods Genes Genetic aspects Genetic engineering Homology Humans Hypoxia Methods Mutagenesis Mutation Non-homologous end joining Plasmids Pluripotency Pluripotent Stem Cells SIX gene family Stem cells Transcription factors Transfection
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