Light-field microscopy with temporal focusing multiphoton illumination for scanless volumetric bioimaging

Light-field microscopy with temporal focusing multiphoton illumination for scanless volumetric bioimaging

A temporal focusing multiphoton illumination (TFMI) method is proposed for achieving selective volume illumination (SVI) (i.e., illuminating only the volume of interest) in light-field microscopy (LFM). The proposed method minimizes the background noise of the LFM images and enhances the contrast, a...

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Veröffentlicht in:Biomedical optics express 2022-12, Vol.13 (12), p.6610-6620
Hauptverfasser: Hsu, Feng-Chun, Lin, Chun-Yu, Hu, Yvonne Yuling, Hwu, Yeu-Kuang, Chiang, Ann-Shyn, Chen, Shean-Jen
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container_end_page 6620
container_issue 12
container_start_page 6610
container_title Biomedical optics express
container_volume 13
creator Hsu, Feng-Chun
Lin, Chun-Yu
Hu, Yvonne Yuling
Hwu, Yeu-Kuang
Chiang, Ann-Shyn
Chen, Shean-Jen
description A temporal focusing multiphoton illumination (TFMI) method is proposed for achieving selective volume illumination (SVI) (i.e., illuminating only the volume of interest) in light-field microscopy (LFM). The proposed method minimizes the background noise of the LFM images and enhances the contrast, and thus improves the imaging quality. Three-dimensional (3D) volumetric imaging is achieved by reconstructing the LFM images using a phase-space deconvolution algorithm. The experimental results obtained using 100-nm fluorescent beads show that the proposed TFMI-LFM system achieves lateral and axial resolutions of 1.2 µm and 1.1 µm, respectively, at the focal plane. Furthermore, the TFMI-LFM system enables 3D images of the single lobe of the drosophila mushroom body with GFP biomarker (OK-107) to be reconstructed in a one-snapshot record.
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title Light-field microscopy with temporal focusing multiphoton illumination for scanless volumetric bioimaging
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