circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis
Circular RNA (circRNA), a type of non-coding RNA, has received a great deal of attention with regard to the initiation and progression of tumors. However, the molecular mechanism and function of circRNAs in breast cancer (BC) remain unclear. In the current study, we discovered that hsa_circ_0028899...
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| Veröffentlicht in: | Cancers 2022-11, Vol.14 (23), p.5862 |
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| creator | Liu, Fei Sang, Yang Zheng, Yang Gu, Lina Meng, Lingjiao Li, Ziyi Dong, Yuyang Wei, Zishuan Geng, Cuizhi Sang, Meixiang |
| description | Circular RNA (circRNA), a type of non-coding RNA, has received a great deal of attention with regard to the initiation and progression of tumors. However, the molecular mechanism and function of circRNAs in breast cancer (BC) remain unclear. In the current study, we discovered that hsa_circ_0028899 (also called circRNF10) was significantly reduced in BC tissues, and a higher level of circRNF10 was markedly related to a favorable prognosis. The results of CCK8, colony formation, Transwell, ELISA, and NK cell-mediated cytotoxicity assays indicated that increased circRNF10 expression could significantly repress the proliferation, invasion, and migration of BC cells and enhance the killing efficiency of NK cells against BC cells. According to these biological functions, the possible role and molecular mechanism of circRNF10 in BC cells were further investigated. We used bioinformatics prediction tools to predict circRNF10-bound miRNAs, which were verified by many experimental studies, including FISH, luciferase reporter assays, RIP, and Western blots. These data suggest that circRNF10 serves as a molecular sponge for miR-934 to further regulate PTEN expression and PI3k/Akt/MICA signaling in vitro and tumor growth in vivo. Altogether, these findings reveal that circRNF10 functions as a novel anti-oncogene in BC via sponging miR-934 and suppressing the PI3K/Akt/MICA pathway. |
| doi_str_mv | 10.3390/cancers14235862 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9739140</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A745273030</galeid><sourcerecordid>A745273030</sourcerecordid><originalsourceid>FETCH-LOGICAL-c488t-c7086e0a879ee8d724337d0df2fb2f3994ab3e0d2662acb361c9270a223161943</originalsourceid><addsrcrecordid>eNptUsFu1DAQjRCIVqVnbsgSFy7pOh6vnVyQllULFaWsVsvZ8jqTrNsk3toO6n4I_4uXltJW2IcZjd9743maLHtb0BOAik6MHgz6UHAG01KwF9kho5LlQlT85aP8IDsO4YqmA1BIIV9nByB4JYHzw-yXsd4sL88KSpbYjp2OGMhq7J23LQ7WkIV3W_TRprIeanKp4-h1R77arkNP5th1-TesbeLVZL6LLrpba2zcEd1qO4RIPnnUKcz_fJbEjXdju0kRSW-XeQV8slidXk4W53Cdz64jmd3a8CZ71egu4PF9PMp-nJ2u5l_yi--fz-ezi9zwsoy5kbQUSHUpK8SylowDyJrWDWvWrIGq4noNSGsmBNNmDaIwFZNUMwaFKCoOR9nHO93tuO6xNjjENJzaettrv1NOW_X0ZbAb1bqfKrlXFZwmgQ_3At7djBii6m0wyRQ9oBuDYnIKQGklIEHfP4NeudEPabyE4uW0ACHLf6hWd6js0LjU1-xF1UzyKZNAYd_25D-odGvsrXEDNjbVnxAmdwTjXQgem4cZC6r2y6SeLVNivHtszQP-7-rAb4kOxQk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2748513678</pqid></control><display><type>article</type><title>circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>PubMed Central</source><creator>Liu, Fei ; Sang, Yang ; Zheng, Yang ; Gu, Lina ; Meng, Lingjiao ; Li, Ziyi ; Dong, Yuyang ; Wei, Zishuan ; Geng, Cuizhi ; Sang, Meixiang</creator><creatorcontrib>Liu, Fei ; Sang, Yang ; Zheng, Yang ; Gu, Lina ; Meng, Lingjiao ; Li, Ziyi ; Dong, Yuyang ; Wei, Zishuan ; Geng, Cuizhi ; Sang, Meixiang</creatorcontrib><description>Circular RNA (circRNA), a type of non-coding RNA, has received a great deal of attention with regard to the initiation and progression of tumors. However, the molecular mechanism and function of circRNAs in breast cancer (BC) remain unclear. In the current study, we discovered that hsa_circ_0028899 (also called circRNF10) was significantly reduced in BC tissues, and a higher level of circRNF10 was markedly related to a favorable prognosis. The results of CCK8, colony formation, Transwell, ELISA, and NK cell-mediated cytotoxicity assays indicated that increased circRNF10 expression could significantly repress the proliferation, invasion, and migration of BC cells and enhance the killing efficiency of NK cells against BC cells. According to these biological functions, the possible role and molecular mechanism of circRNF10 in BC cells were further investigated. We used bioinformatics prediction tools to predict circRNF10-bound miRNAs, which were verified by many experimental studies, including FISH, luciferase reporter assays, RIP, and Western blots. These data suggest that circRNF10 serves as a molecular sponge for miR-934 to further regulate PTEN expression and PI3k/Akt/MICA signaling in vitro and tumor growth in vivo. Altogether, these findings reveal that circRNF10 functions as a novel anti-oncogene in BC via sponging miR-934 and suppressing the PI3K/Akt/MICA pathway.</description><identifier>ISSN: 2072-6694</identifier><identifier>EISSN: 2072-6694</identifier><identifier>DOI: 10.3390/cancers14235862</identifier><identifier>PMID: 36497344</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>1-Phosphatidylinositol 3-kinase ; AKT protein ; Binding sites ; Bioinformatics ; Biotechnology ; Breast cancer ; Cancer therapies ; Cell migration ; Cell proliferation ; Circular RNA ; Cytotoxicity ; Development and progression ; Enzyme-linked immunosorbent assay ; Gene expression ; Killer cells ; Medical prognosis ; MicroRNA ; MicroRNAs ; Natural killer cells ; Prognosis ; PTEN protein ; Reagents ; Signal transduction ; Tumors ; Western blotting</subject><ispartof>Cancers, 2022-11, Vol.14 (23), p.5862</ispartof><rights>COPYRIGHT 2022 MDPI AG</rights><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-c7086e0a879ee8d724337d0df2fb2f3994ab3e0d2662acb361c9270a223161943</citedby><cites>FETCH-LOGICAL-c488t-c7086e0a879ee8d724337d0df2fb2f3994ab3e0d2662acb361c9270a223161943</cites><orcidid>0000-0002-7013-0981</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739140/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9739140/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36497344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Fei</creatorcontrib><creatorcontrib>Sang, Yang</creatorcontrib><creatorcontrib>Zheng, Yang</creatorcontrib><creatorcontrib>Gu, Lina</creatorcontrib><creatorcontrib>Meng, Lingjiao</creatorcontrib><creatorcontrib>Li, Ziyi</creatorcontrib><creatorcontrib>Dong, Yuyang</creatorcontrib><creatorcontrib>Wei, Zishuan</creatorcontrib><creatorcontrib>Geng, Cuizhi</creatorcontrib><creatorcontrib>Sang, Meixiang</creatorcontrib><title>circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis</title><title>Cancers</title><addtitle>Cancers (Basel)</addtitle><description>Circular RNA (circRNA), a type of non-coding RNA, has received a great deal of attention with regard to the initiation and progression of tumors. However, the molecular mechanism and function of circRNAs in breast cancer (BC) remain unclear. In the current study, we discovered that hsa_circ_0028899 (also called circRNF10) was significantly reduced in BC tissues, and a higher level of circRNF10 was markedly related to a favorable prognosis. The results of CCK8, colony formation, Transwell, ELISA, and NK cell-mediated cytotoxicity assays indicated that increased circRNF10 expression could significantly repress the proliferation, invasion, and migration of BC cells and enhance the killing efficiency of NK cells against BC cells. According to these biological functions, the possible role and molecular mechanism of circRNF10 in BC cells were further investigated. We used bioinformatics prediction tools to predict circRNF10-bound miRNAs, which were verified by many experimental studies, including FISH, luciferase reporter assays, RIP, and Western blots. These data suggest that circRNF10 serves as a molecular sponge for miR-934 to further regulate PTEN expression and PI3k/Akt/MICA signaling in vitro and tumor growth in vivo. Altogether, these findings reveal that circRNF10 functions as a novel anti-oncogene in BC via sponging miR-934 and suppressing the PI3K/Akt/MICA pathway.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>AKT protein</subject><subject>Binding sites</subject><subject>Bioinformatics</subject><subject>Biotechnology</subject><subject>Breast cancer</subject><subject>Cancer therapies</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Circular RNA</subject><subject>Cytotoxicity</subject><subject>Development and progression</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Gene expression</subject><subject>Killer cells</subject><subject>Medical prognosis</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>Natural killer cells</subject><subject>Prognosis</subject><subject>PTEN protein</subject><subject>Reagents</subject><subject>Signal transduction</subject><subject>Tumors</subject><subject>Western blotting</subject><issn>2072-6694</issn><issn>2072-6694</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptUsFu1DAQjRCIVqVnbsgSFy7pOh6vnVyQllULFaWsVsvZ8jqTrNsk3toO6n4I_4uXltJW2IcZjd9743maLHtb0BOAik6MHgz6UHAG01KwF9kho5LlQlT85aP8IDsO4YqmA1BIIV9nByB4JYHzw-yXsd4sL88KSpbYjp2OGMhq7J23LQ7WkIV3W_TRprIeanKp4-h1R77arkNP5th1-TesbeLVZL6LLrpba2zcEd1qO4RIPnnUKcz_fJbEjXdju0kRSW-XeQV8slidXk4W53Cdz64jmd3a8CZ71egu4PF9PMp-nJ2u5l_yi--fz-ezi9zwsoy5kbQUSHUpK8SylowDyJrWDWvWrIGq4noNSGsmBNNmDaIwFZNUMwaFKCoOR9nHO93tuO6xNjjENJzaettrv1NOW_X0ZbAb1bqfKrlXFZwmgQ_3At7djBii6m0wyRQ9oBuDYnIKQGklIEHfP4NeudEPabyE4uW0ACHLf6hWd6js0LjU1-xF1UzyKZNAYd_25D-odGvsrXEDNjbVnxAmdwTjXQgem4cZC6r2y6SeLVNivHtszQP-7-rAb4kOxQk</recordid><startdate>20221128</startdate><enddate>20221128</enddate><creator>Liu, Fei</creator><creator>Sang, Yang</creator><creator>Zheng, Yang</creator><creator>Gu, Lina</creator><creator>Meng, Lingjiao</creator><creator>Li, Ziyi</creator><creator>Dong, Yuyang</creator><creator>Wei, Zishuan</creator><creator>Geng, Cuizhi</creator><creator>Sang, Meixiang</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7013-0981</orcidid></search><sort><creationdate>20221128</creationdate><title>circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis</title><author>Liu, Fei ; Sang, Yang ; Zheng, Yang ; Gu, Lina ; Meng, Lingjiao ; Li, Ziyi ; Dong, Yuyang ; Wei, Zishuan ; Geng, Cuizhi ; Sang, Meixiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-c7086e0a879ee8d724337d0df2fb2f3994ab3e0d2662acb361c9270a223161943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>AKT protein</topic><topic>Binding sites</topic><topic>Bioinformatics</topic><topic>Biotechnology</topic><topic>Breast cancer</topic><topic>Cancer therapies</topic><topic>Cell migration</topic><topic>Cell proliferation</topic><topic>Circular RNA</topic><topic>Cytotoxicity</topic><topic>Development and progression</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Gene expression</topic><topic>Killer cells</topic><topic>Medical prognosis</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>Natural killer cells</topic><topic>Prognosis</topic><topic>PTEN protein</topic><topic>Reagents</topic><topic>Signal transduction</topic><topic>Tumors</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Fei</creatorcontrib><creatorcontrib>Sang, Yang</creatorcontrib><creatorcontrib>Zheng, Yang</creatorcontrib><creatorcontrib>Gu, Lina</creatorcontrib><creatorcontrib>Meng, Lingjiao</creatorcontrib><creatorcontrib>Li, Ziyi</creatorcontrib><creatorcontrib>Dong, Yuyang</creatorcontrib><creatorcontrib>Wei, Zishuan</creatorcontrib><creatorcontrib>Geng, Cuizhi</creatorcontrib><creatorcontrib>Sang, Meixiang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Fei</au><au>Sang, Yang</au><au>Zheng, Yang</au><au>Gu, Lina</au><au>Meng, Lingjiao</au><au>Li, Ziyi</au><au>Dong, Yuyang</au><au>Wei, Zishuan</au><au>Geng, Cuizhi</au><au>Sang, Meixiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis</atitle><jtitle>Cancers</jtitle><addtitle>Cancers (Basel)</addtitle><date>2022-11-28</date><risdate>2022</risdate><volume>14</volume><issue>23</issue><spage>5862</spage><pages>5862-</pages><issn>2072-6694</issn><eissn>2072-6694</eissn><abstract>Circular RNA (circRNA), a type of non-coding RNA, has received a great deal of attention with regard to the initiation and progression of tumors. However, the molecular mechanism and function of circRNAs in breast cancer (BC) remain unclear. In the current study, we discovered that hsa_circ_0028899 (also called circRNF10) was significantly reduced in BC tissues, and a higher level of circRNF10 was markedly related to a favorable prognosis. The results of CCK8, colony formation, Transwell, ELISA, and NK cell-mediated cytotoxicity assays indicated that increased circRNF10 expression could significantly repress the proliferation, invasion, and migration of BC cells and enhance the killing efficiency of NK cells against BC cells. According to these biological functions, the possible role and molecular mechanism of circRNF10 in BC cells were further investigated. We used bioinformatics prediction tools to predict circRNF10-bound miRNAs, which were verified by many experimental studies, including FISH, luciferase reporter assays, RIP, and Western blots. These data suggest that circRNF10 serves as a molecular sponge for miR-934 to further regulate PTEN expression and PI3k/Akt/MICA signaling in vitro and tumor growth in vivo. Altogether, these findings reveal that circRNF10 functions as a novel anti-oncogene in BC via sponging miR-934 and suppressing the PI3K/Akt/MICA pathway.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>36497344</pmid><doi>10.3390/cancers14235862</doi><orcidid>https://orcid.org/0000-0002-7013-0981</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 1-Phosphatidylinositol 3-kinase AKT protein Binding sites Bioinformatics Biotechnology Breast cancer Cancer therapies Cell migration Cell proliferation Circular RNA Cytotoxicity Development and progression Enzyme-linked immunosorbent assay Gene expression Killer cells Medical prognosis MicroRNA MicroRNAs Natural killer cells Prognosis PTEN protein Reagents Signal transduction Tumors Western blotting |
| title | circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis |
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