Establishment of a reverse genetics system for rabies virus strain Komatsugawa
The rabies virus strain Komatsugawa isolated from a dog in Tokyo in the 1940s retains biological properties as a field strain, providing an effective model for studying rabies pathogenesis. To facilitate molecular studies on the pathogenesis, this study aimed to establish a reverse genetics system f...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2022, Vol.84(11), pp.1508-1513 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | TAKAHASHI, Tatsuki INUKAI, Maho IZUMI, Fumiki FUJII, Yuji NISHIYAMA, Shoko MASATANI, Tatsunori SUGIYAMA, Makoto ITO, Naoto |
| description | The rabies virus strain Komatsugawa isolated from a dog in Tokyo in the 1940s retains biological properties as a field strain, providing an effective model for studying rabies pathogenesis. To facilitate molecular studies on the pathogenesis, this study aimed to establish a reverse genetics system for the Komatsugawa strain. By transfecting the full-length genome plasmid of this strain, infectious virus with artificially introduced genetic markers in its genome was rescued. The recombinant strain had biological properties similar to those of the original strain. These findings indicate that a reverse genetics system for the Komatsugawa strain has successfully been established. |
| doi_str_mv | 10.1292/jvms.22-0254 |
| format | Article |
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To facilitate molecular studies on the pathogenesis, this study aimed to establish a reverse genetics system for the Komatsugawa strain. By transfecting the full-length genome plasmid of this strain, infectious virus with artificially introduced genetic markers in its genome was rescued. The recombinant strain had biological properties similar to those of the original strain. These findings indicate that a reverse genetics system for the Komatsugawa strain has successfully been established.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.22-0254</identifier><identifier>PMID: 36171109</identifier><language>eng</language><publisher>Tokyo: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Genetic markers ; Genomes ; Japan ; Komatsugawa ; Lyssavirus ; Pathogenesis ; Public Health ; Rabies ; rabies virus ; reverse genetics ; street strain ; Viruses</subject><ispartof>Journal of Veterinary Medical Science, 2022, Vol.84(11), pp.1508-1513</ispartof><rights>2022 by the Japanese Society of Veterinary Science</rights><rights>2022. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 The Japanese Society of Veterinary Science 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c483t-95e22e885c29fae483b760b1882cddfff766ac13aaf615ec5013b79da85ffe93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705820/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9705820/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>TAKAHASHI, Tatsuki</creatorcontrib><creatorcontrib>INUKAI, Maho</creatorcontrib><creatorcontrib>IZUMI, Fumiki</creatorcontrib><creatorcontrib>FUJII, Yuji</creatorcontrib><creatorcontrib>NISHIYAMA, Shoko</creatorcontrib><creatorcontrib>MASATANI, Tatsunori</creatorcontrib><creatorcontrib>SUGIYAMA, Makoto</creatorcontrib><creatorcontrib>ITO, Naoto</creatorcontrib><title>Establishment of a reverse genetics system for rabies virus strain Komatsugawa</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>The rabies virus strain Komatsugawa isolated from a dog in Tokyo in the 1940s retains biological properties as a field strain, providing an effective model for studying rabies pathogenesis. To facilitate molecular studies on the pathogenesis, this study aimed to establish a reverse genetics system for the Komatsugawa strain. By transfecting the full-length genome plasmid of this strain, infectious virus with artificially introduced genetic markers in its genome was rescued. The recombinant strain had biological properties similar to those of the original strain. These findings indicate that a reverse genetics system for the Komatsugawa strain has successfully been established.</description><subject>Genetic markers</subject><subject>Genomes</subject><subject>Japan</subject><subject>Komatsugawa</subject><subject>Lyssavirus</subject><subject>Pathogenesis</subject><subject>Public Health</subject><subject>Rabies</subject><subject>rabies virus</subject><subject>reverse genetics</subject><subject>street strain</subject><subject>Viruses</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpdkUFr3DAQhUVoSbZpb_0Bgl56iFNJtizpUihL0pSG9pK7GGtHu1psK5XsLfn3ldllobmMYOabx9M8Qj5ydsuFEV_2hyHfClExIZsLsuJ1oyrV1OYNWTHD20oJya7Iu5z3jAnetOaSXNUtV5wzsyK_7vIEXR_ybsBxotFToAkPmDLSLY44BZdpfskTDtTHRBN0ATM9hDSX_pQgjPRnHGDK8xb-wnvy1kOf8cPpvSZP93dP64fq8ff3H-tvj5VrdD1VRqIQqLV0wnjA0utUyzqutXCbjfdetS04XgP4lkt0kvFCmA1o6T2a-pp8Pco-z92AG1esJ-jtcwoDpBcbIdj_J2PY2W08WKOY1IIVgc8ngRT_zJgnO4TssO9hxDhnKxQ3TeG0KuinV-g-zmksvyuUbKQ2tV4c3Rwpl2LOCf3ZDGd2yckuOVkh7JJTwddHfF_Ov8UzDKkcvMcjrBvL-VJPW-ep20GyONb_AGWenv0</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>TAKAHASHI, Tatsuki</creator><creator>INUKAI, Maho</creator><creator>IZUMI, Fumiki</creator><creator>FUJII, Yuji</creator><creator>NISHIYAMA, Shoko</creator><creator>MASATANI, Tatsunori</creator><creator>SUGIYAMA, Makoto</creator><creator>ITO, Naoto</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2022</creationdate><title>Establishment of a reverse genetics system for rabies virus strain Komatsugawa</title><author>TAKAHASHI, Tatsuki ; INUKAI, Maho ; IZUMI, Fumiki ; FUJII, Yuji ; NISHIYAMA, Shoko ; MASATANI, Tatsunori ; SUGIYAMA, Makoto ; ITO, Naoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-95e22e885c29fae483b760b1882cddfff766ac13aaf615ec5013b79da85ffe93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Genetic markers</topic><topic>Genomes</topic><topic>Japan</topic><topic>Komatsugawa</topic><topic>Lyssavirus</topic><topic>Pathogenesis</topic><topic>Public Health</topic><topic>Rabies</topic><topic>rabies virus</topic><topic>reverse genetics</topic><topic>street strain</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TAKAHASHI, Tatsuki</creatorcontrib><creatorcontrib>INUKAI, Maho</creatorcontrib><creatorcontrib>IZUMI, Fumiki</creatorcontrib><creatorcontrib>FUJII, Yuji</creatorcontrib><creatorcontrib>NISHIYAMA, Shoko</creatorcontrib><creatorcontrib>MASATANI, Tatsunori</creatorcontrib><creatorcontrib>SUGIYAMA, Makoto</creatorcontrib><creatorcontrib>ITO, Naoto</creatorcontrib><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TAKAHASHI, Tatsuki</au><au>INUKAI, Maho</au><au>IZUMI, Fumiki</au><au>FUJII, Yuji</au><au>NISHIYAMA, Shoko</au><au>MASATANI, Tatsunori</au><au>SUGIYAMA, Makoto</au><au>ITO, Naoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of a reverse genetics system for rabies virus strain Komatsugawa</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2022</date><risdate>2022</risdate><volume>84</volume><issue>11</issue><spage>1508</spage><epage>1513</epage><pages>1508-1513</pages><artnum>22-0254</artnum><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>The rabies virus strain Komatsugawa isolated from a dog in Tokyo in the 1940s retains biological properties as a field strain, providing an effective model for studying rabies pathogenesis. To facilitate molecular studies on the pathogenesis, this study aimed to establish a reverse genetics system for the Komatsugawa strain. By transfecting the full-length genome plasmid of this strain, infectious virus with artificially introduced genetic markers in its genome was rescued. The recombinant strain had biological properties similar to those of the original strain. 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| subjects | Genetic markers Genomes Japan Komatsugawa Lyssavirus Pathogenesis Public Health Rabies rabies virus reverse genetics street strain Viruses |
| title | Establishment of a reverse genetics system for rabies virus strain Komatsugawa |
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