Proteomics of Salt Gland-Secreted Sap Indicates a Pivotal Role for Vesicle Transport and Energy Metabolism in Plant Salt Secretion
Soil salinization is one of the major factors restricting crop growth and agricultural production worldwide. Recretohalophytes have developed unique epidermal structures in their aboveground tissues, such as salt glands or salt bladders, to secrete excess salt out of the plant body as a protective m...
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| Veröffentlicht in: | International journal of molecular sciences 2022-11, Vol.23 (22), p.13885 |
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| creator | Lu, Chaoxia Zhang, Yuanyuan Mi, Ping Guo, Xueying Wen, Yixuan Han, Guoliang Wang, Baoshan |
| description | Soil salinization is one of the major factors restricting crop growth and agricultural production worldwide. Recretohalophytes have developed unique epidermal structures in their aboveground tissues, such as salt glands or salt bladders, to secrete excess salt out of the plant body as a protective mechanism from ion damage. Three hypotheses were proposed to explain how salt glands secrete salts: the osmotic hypothesis, a hypothesis similar to animal fluid transport, and vesicle-mediated exocytosis. However, there is no direct evidence to show whether the salt gland-secreted liquid contains landmark proteins or peptides which would elucidate the salt secretion mechanism. In this study, we collected the secreted liquid of salt glands from
, followed by extraction and identification of its constituent proteins and peptides by SDS-PAGE and mass spectrometry. We detected 214 proteins and 440 polypeptides in the salt gland-secreted droplets of plants grown under control conditions. Unexpectedly, the proportion of energy metabolism-related proteins increased significantly though only 16 proteins and 35 polypeptides in the droplets of salt-treated plants were detected. In addition, vesicle transport proteins such as the Golgi marker enzyme glycosyltransferase were present in the secreted sap of salt glands from both control and salt-treated plants. These results suggest that trans-Golgi network-mediated vesicular transport and energy production contributes to salt secretion in salt glands. |
| doi_str_mv | 10.3390/ijms232213885 |
| format | Article |
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, followed by extraction and identification of its constituent proteins and peptides by SDS-PAGE and mass spectrometry. We detected 214 proteins and 440 polypeptides in the salt gland-secreted droplets of plants grown under control conditions. Unexpectedly, the proportion of energy metabolism-related proteins increased significantly though only 16 proteins and 35 polypeptides in the droplets of salt-treated plants were detected. In addition, vesicle transport proteins such as the Golgi marker enzyme glycosyltransferase were present in the secreted sap of salt glands from both control and salt-treated plants. These results suggest that trans-Golgi network-mediated vesicular transport and energy production contributes to salt secretion in salt glands.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms232213885</identifier><identifier>PMID: 36430364</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Abiotic stress ; Agricultural production ; Animals ; Chloroplasts ; Crop growth ; Droplets ; Energy Metabolism ; Exocytosis ; Gel electrophoresis ; Glycosyltransferase ; Golgi apparatus ; Hypotheses ; Mass spectrometry ; Mass spectroscopy ; Peptides ; Plant Leaves - metabolism ; Polypeptides ; Protein transport ; Proteins ; Proteomics ; Salt ; Salt gland ; Salt Gland - metabolism ; Scientific imaging ; Secretion ; Sodium Chloride - metabolism ; Sodium Chloride, Dietary - metabolism</subject><ispartof>International journal of molecular sciences, 2022-11, Vol.23 (22), p.13885</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-97aeeb5ac127ccb640e096ff3a3dab79c8695b6cf97dc0d5c26bf63f4476074f3</citedby><cites>FETCH-LOGICAL-c415t-97aeeb5ac127ccb640e096ff3a3dab79c8695b6cf97dc0d5c26bf63f4476074f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693062/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693062/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36430364$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Chaoxia</creatorcontrib><creatorcontrib>Zhang, Yuanyuan</creatorcontrib><creatorcontrib>Mi, Ping</creatorcontrib><creatorcontrib>Guo, Xueying</creatorcontrib><creatorcontrib>Wen, Yixuan</creatorcontrib><creatorcontrib>Han, Guoliang</creatorcontrib><creatorcontrib>Wang, Baoshan</creatorcontrib><title>Proteomics of Salt Gland-Secreted Sap Indicates a Pivotal Role for Vesicle Transport and Energy Metabolism in Plant Salt Secretion</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Soil salinization is one of the major factors restricting crop growth and agricultural production worldwide. Recretohalophytes have developed unique epidermal structures in their aboveground tissues, such as salt glands or salt bladders, to secrete excess salt out of the plant body as a protective mechanism from ion damage. Three hypotheses were proposed to explain how salt glands secrete salts: the osmotic hypothesis, a hypothesis similar to animal fluid transport, and vesicle-mediated exocytosis. However, there is no direct evidence to show whether the salt gland-secreted liquid contains landmark proteins or peptides which would elucidate the salt secretion mechanism. In this study, we collected the secreted liquid of salt glands from
, followed by extraction and identification of its constituent proteins and peptides by SDS-PAGE and mass spectrometry. We detected 214 proteins and 440 polypeptides in the salt gland-secreted droplets of plants grown under control conditions. Unexpectedly, the proportion of energy metabolism-related proteins increased significantly though only 16 proteins and 35 polypeptides in the droplets of salt-treated plants were detected. In addition, vesicle transport proteins such as the Golgi marker enzyme glycosyltransferase were present in the secreted sap of salt glands from both control and salt-treated plants. These results suggest that trans-Golgi network-mediated vesicular transport and energy production contributes to salt secretion in salt glands.</description><subject>Abiotic stress</subject><subject>Agricultural production</subject><subject>Animals</subject><subject>Chloroplasts</subject><subject>Crop growth</subject><subject>Droplets</subject><subject>Energy Metabolism</subject><subject>Exocytosis</subject><subject>Gel electrophoresis</subject><subject>Glycosyltransferase</subject><subject>Golgi apparatus</subject><subject>Hypotheses</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Peptides</subject><subject>Plant Leaves - metabolism</subject><subject>Polypeptides</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Salt</subject><subject>Salt gland</subject><subject>Salt Gland - metabolism</subject><subject>Scientific imaging</subject><subject>Secretion</subject><subject>Sodium Chloride - metabolism</subject><subject>Sodium Chloride, Dietary - metabolism</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkcFPHCEUxkljU1frsVdD4sXLWAYYZriYNMaqyTZuqvVKGOZh2czAFlgTr_3LpVlrdr3wXng_Pt6XD6EvNTljTJKvbjklyiitWdc1H9Cs5pRWhIh2b6vfRwcpLQkpYCM_oX0mOCPlmKG_ixgyhMmZhIPFd3rM-GrUfqjuwETIMJS7Fb7xgzM6Q8IaL9xTyHrEP8MI2IaIHyA5U_r7qH1ahZhxeY8vPcTHZ_wDsu7D6NKEnceLIp03v2z0XfCf0UerxwRHr_UQ_fp-eX9xXc1vr24uvs0rw-smV7LVAH2jTU1bY3rBCRAprGWaDbpvpemEbHphrGwHQ4bGUNFbwSznrSAtt-wQnW90V-t-gsGAz1GPahXdpOOzCtqp3Yl3v9VjeFJSSEYELQKnrwIx_FlDympyycBYPEFYJ0VbThrS1R0p6Mk7dBnW0Rd7hWKSl6U6VqhqQ5kYUopg35apifqXrtpJt_DH2w7e6P9xshfhm6Ku</recordid><startdate>20221111</startdate><enddate>20221111</enddate><creator>Lu, Chaoxia</creator><creator>Zhang, Yuanyuan</creator><creator>Mi, Ping</creator><creator>Guo, Xueying</creator><creator>Wen, Yixuan</creator><creator>Han, Guoliang</creator><creator>Wang, Baoshan</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20221111</creationdate><title>Proteomics of Salt Gland-Secreted Sap Indicates a Pivotal Role for Vesicle Transport and Energy Metabolism in Plant Salt Secretion</title><author>Lu, Chaoxia ; Zhang, Yuanyuan ; Mi, Ping ; Guo, Xueying ; Wen, Yixuan ; Han, Guoliang ; Wang, Baoshan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-97aeeb5ac127ccb640e096ff3a3dab79c8695b6cf97dc0d5c26bf63f4476074f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Abiotic stress</topic><topic>Agricultural production</topic><topic>Animals</topic><topic>Chloroplasts</topic><topic>Crop growth</topic><topic>Droplets</topic><topic>Energy Metabolism</topic><topic>Exocytosis</topic><topic>Gel electrophoresis</topic><topic>Glycosyltransferase</topic><topic>Golgi apparatus</topic><topic>Hypotheses</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Peptides</topic><topic>Plant Leaves - metabolism</topic><topic>Polypeptides</topic><topic>Protein transport</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Salt</topic><topic>Salt gland</topic><topic>Salt Gland - metabolism</topic><topic>Scientific imaging</topic><topic>Secretion</topic><topic>Sodium Chloride - metabolism</topic><topic>Sodium Chloride, Dietary - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Chaoxia</creatorcontrib><creatorcontrib>Zhang, Yuanyuan</creatorcontrib><creatorcontrib>Mi, Ping</creatorcontrib><creatorcontrib>Guo, Xueying</creatorcontrib><creatorcontrib>Wen, Yixuan</creatorcontrib><creatorcontrib>Han, Guoliang</creatorcontrib><creatorcontrib>Wang, Baoshan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Chaoxia</au><au>Zhang, Yuanyuan</au><au>Mi, Ping</au><au>Guo, Xueying</au><au>Wen, Yixuan</au><au>Han, Guoliang</au><au>Wang, Baoshan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomics of Salt Gland-Secreted Sap Indicates a Pivotal Role for Vesicle Transport and Energy Metabolism in Plant Salt Secretion</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2022-11-11</date><risdate>2022</risdate><volume>23</volume><issue>22</issue><spage>13885</spage><pages>13885-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Soil salinization is one of the major factors restricting crop growth and agricultural production worldwide. Recretohalophytes have developed unique epidermal structures in their aboveground tissues, such as salt glands or salt bladders, to secrete excess salt out of the plant body as a protective mechanism from ion damage. Three hypotheses were proposed to explain how salt glands secrete salts: the osmotic hypothesis, a hypothesis similar to animal fluid transport, and vesicle-mediated exocytosis. However, there is no direct evidence to show whether the salt gland-secreted liquid contains landmark proteins or peptides which would elucidate the salt secretion mechanism. In this study, we collected the secreted liquid of salt glands from
, followed by extraction and identification of its constituent proteins and peptides by SDS-PAGE and mass spectrometry. We detected 214 proteins and 440 polypeptides in the salt gland-secreted droplets of plants grown under control conditions. Unexpectedly, the proportion of energy metabolism-related proteins increased significantly though only 16 proteins and 35 polypeptides in the droplets of salt-treated plants were detected. In addition, vesicle transport proteins such as the Golgi marker enzyme glycosyltransferase were present in the secreted sap of salt glands from both control and salt-treated plants. These results suggest that trans-Golgi network-mediated vesicular transport and energy production contributes to salt secretion in salt glands.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>36430364</pmid><doi>10.3390/ijms232213885</doi><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Abiotic stress Agricultural production Animals Chloroplasts Crop growth Droplets Energy Metabolism Exocytosis Gel electrophoresis Glycosyltransferase Golgi apparatus Hypotheses Mass spectrometry Mass spectroscopy Peptides Plant Leaves - metabolism Polypeptides Protein transport Proteins Proteomics Salt Salt gland Salt Gland - metabolism Scientific imaging Secretion Sodium Chloride - metabolism Sodium Chloride, Dietary - metabolism |
| title | Proteomics of Salt Gland-Secreted Sap Indicates a Pivotal Role for Vesicle Transport and Energy Metabolism in Plant Salt Secretion |
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