Isothiocyanate-Rich Extracts from Cauliflower ( Brassica oleracea Var. Botrytis) and Radish ( Raphanus sativus ) Inhibited Metabolic Activity and Induced ROS in Selected Human HCT116 and HT-29 Colorectal Cancer Cells
Cruciferous vegetables such as cauliflower and radish contain isothiocyanates exhibiting chemoprotective effects in vitro and in vivo. This research aimed to assess the impact of cauliflower (CIE) and radish (RIE) isothiocyanate extracts on the metabolic activity, intracellular reactive oxygen speci...
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| Veröffentlicht in: | International journal of environmental research and public health 2022-11, Vol.19 (22), p.14919 |
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| creator | Cuellar-Nuñez, Mardey Liceth Luzardo-Ocampo, Ivan Lee-Martínez, Sarah Larrauri-Rodríguez, Michelle Zaldívar-Lelo de Larrea, Guadalupe Pérez-Serrano, Rosa Martha Camacho-Calderón, Nicolás |
| description | Cruciferous vegetables such as cauliflower and radish contain isothiocyanates exhibiting chemoprotective effects in vitro and in vivo. This research aimed to assess the impact of cauliflower (CIE) and radish (RIE) isothiocyanate extracts on the metabolic activity, intracellular reactive oxygen species (ROS), and LDH production of selected human colorectal adenocarcinoma cells (HCT116 and HT-29 for early and late colon cancer development, respectively). Non-cancerous colon cells (CCD-33Co) were used as a cytotoxicity control. The CIE samples displayed the highest allyl isothiocyanate (AITC: 12.55 µg/g) contents, whereas RIE was the most abundant in benzyl isothiocyanate (BITC: 15.35 µg/g). Both extracts effectively inhibited HCT116 and HT-29 metabolic activity, but the CIE impact was higher than that of RIE on HCT116 (IC
: 0.56 mg/mL). Assays using the half-inhibitory concentrations (IC
) of all treatments, including AITC and BITC, displayed increased (
< 0.05) LDH (absorbance: 0.25-0.40 nm) and ROS release (1190-1697 relative fluorescence units) in both cell lines. BITC showed the highest in silico binding affinity with all the tested colorectal cancer molecular markers (NF-kB, β-catenin, and NRF2-NFE2). The theoretical evaluation of AITC and BITC bioavailability showed high values for both compounds. The results indicate that CIE and RIE extracts display chemopreventive effects in vitro, but additional experiments are needed to validate their effects. |
| doi_str_mv | 10.3390/ijerph192214919 |
| format | Article |
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: 0.56 mg/mL). Assays using the half-inhibitory concentrations (IC
) of all treatments, including AITC and BITC, displayed increased (
< 0.05) LDH (absorbance: 0.25-0.40 nm) and ROS release (1190-1697 relative fluorescence units) in both cell lines. BITC showed the highest in silico binding affinity with all the tested colorectal cancer molecular markers (NF-kB, β-catenin, and NRF2-NFE2). The theoretical evaluation of AITC and BITC bioavailability showed high values for both compounds. The results indicate that CIE and RIE extracts display chemopreventive effects in vitro, but additional experiments are needed to validate their effects.</description><identifier>ISSN: 1660-4601</identifier><identifier>ISSN: 1661-7827</identifier><identifier>EISSN: 1660-4601</identifier><identifier>DOI: 10.3390/ijerph192214919</identifier><identifier>PMID: 36429638</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adenocarcinoma ; Allyl isothiocyanate ; Apoptosis ; Bioavailability ; Biocompatibility ; Botrytis ; Brassica ; Cauliflowers ; Cell cycle ; Colon ; Colon cancer ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - drug therapy ; Cytotoxicity ; DNA methylation ; Fluorescence ; Humans ; Isothiocyanate ; Isothiocyanates - pharmacology ; Metabolism ; NF-κB protein ; Radishes ; Raphanus ; Reactive Oxygen Species ; Vegetables ; β-Catenin</subject><ispartof>International journal of environmental research and public health, 2022-11, Vol.19 (22), p.14919</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-41a94bc3a10deaabfad8f4126baee3c84ecbf42d100d4c4e70040de10f8b9bdd3</citedby><cites>FETCH-LOGICAL-c351t-41a94bc3a10deaabfad8f4126baee3c84ecbf42d100d4c4e70040de10f8b9bdd3</cites><orcidid>0000-0002-8033-3520 ; 0000-0003-2045-8951 ; 0000-0003-0238-1559</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691161/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691161/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36429638$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cuellar-Nuñez, Mardey Liceth</creatorcontrib><creatorcontrib>Luzardo-Ocampo, Ivan</creatorcontrib><creatorcontrib>Lee-Martínez, Sarah</creatorcontrib><creatorcontrib>Larrauri-Rodríguez, Michelle</creatorcontrib><creatorcontrib>Zaldívar-Lelo de Larrea, Guadalupe</creatorcontrib><creatorcontrib>Pérez-Serrano, Rosa Martha</creatorcontrib><creatorcontrib>Camacho-Calderón, Nicolás</creatorcontrib><title>Isothiocyanate-Rich Extracts from Cauliflower ( Brassica oleracea Var. Botrytis) and Radish ( Raphanus sativus ) Inhibited Metabolic Activity and Induced ROS in Selected Human HCT116 and HT-29 Colorectal Cancer Cells</title><title>International journal of environmental research and public health</title><addtitle>Int J Environ Res Public Health</addtitle><description>Cruciferous vegetables such as cauliflower and radish contain isothiocyanates exhibiting chemoprotective effects in vitro and in vivo. This research aimed to assess the impact of cauliflower (CIE) and radish (RIE) isothiocyanate extracts on the metabolic activity, intracellular reactive oxygen species (ROS), and LDH production of selected human colorectal adenocarcinoma cells (HCT116 and HT-29 for early and late colon cancer development, respectively). Non-cancerous colon cells (CCD-33Co) were used as a cytotoxicity control. The CIE samples displayed the highest allyl isothiocyanate (AITC: 12.55 µg/g) contents, whereas RIE was the most abundant in benzyl isothiocyanate (BITC: 15.35 µg/g). Both extracts effectively inhibited HCT116 and HT-29 metabolic activity, but the CIE impact was higher than that of RIE on HCT116 (IC
: 0.56 mg/mL). Assays using the half-inhibitory concentrations (IC
) of all treatments, including AITC and BITC, displayed increased (
< 0.05) LDH (absorbance: 0.25-0.40 nm) and ROS release (1190-1697 relative fluorescence units) in both cell lines. BITC showed the highest in silico binding affinity with all the tested colorectal cancer molecular markers (NF-kB, β-catenin, and NRF2-NFE2). The theoretical evaluation of AITC and BITC bioavailability showed high values for both compounds. The results indicate that CIE and RIE extracts display chemopreventive effects in vitro, but additional experiments are needed to validate their effects.</description><subject>Adenocarcinoma</subject><subject>Allyl isothiocyanate</subject><subject>Apoptosis</subject><subject>Bioavailability</subject><subject>Biocompatibility</subject><subject>Botrytis</subject><subject>Brassica</subject><subject>Cauliflowers</subject><subject>Cell cycle</subject><subject>Colon</subject><subject>Colon cancer</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Colorectal Neoplasms - drug therapy</subject><subject>Cytotoxicity</subject><subject>DNA methylation</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>Isothiocyanate</subject><subject>Isothiocyanates - pharmacology</subject><subject>Metabolism</subject><subject>NF-κB protein</subject><subject>Radishes</subject><subject>Raphanus</subject><subject>Reactive Oxygen Species</subject><subject>Vegetables</subject><subject>β-Catenin</subject><issn>1660-4601</issn><issn>1661-7827</issn><issn>1660-4601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkk9vEzEQxVcIREvhzA1Z4tIetrXXzia-ILWrlkQqqpQGrqtZe5Z15NjB9hbyTftxcP9QlZ7G0vzm6T3rFcVHRo85l_TErDFsByarignJ5Ktin9U1LUVN2etn773iXYxrSvlM1PJtscdrUcmaz_aL20X0aTBe7cBBwnJp1EDO_6QAKkXSB78hDYzW9Nb_xkAOyVmAGI0C4i1mCIH8gHBMznwKu2TiEQGnyRK0iUOml7AdwI2RREjmJs8jsnCD6UxCTb5hgs5bo8ipyluTdvfHC6dHldfLq2tiHLlGi-oOn48bcGTerBir78H5qqwkabz1IRNgs1OnsskGrY3vizc92IgfHudB8f3ifNXMy8urr4vm9LJUfMJSKRhI0SkOjGoE6HrQs16wqu4AkauZQNX1otKMUi2UwCmlIpOM9rNOdlrzg-LLg-527DaoFbr8d7bdBrOBsGs9mPb_jTND-9PftLKWOQjLAoePAsH_GjGmdmOiyhHAoR9jW00FnbBJxaqMfn6Brv0YXI6XKS5FVYspzdTJA6WCjzFg_2SG0fauNe2L1uSLT88zPPH_asL_Aix-wdg</recordid><startdate>20221113</startdate><enddate>20221113</enddate><creator>Cuellar-Nuñez, Mardey Liceth</creator><creator>Luzardo-Ocampo, Ivan</creator><creator>Lee-Martínez, Sarah</creator><creator>Larrauri-Rodríguez, Michelle</creator><creator>Zaldívar-Lelo de Larrea, Guadalupe</creator><creator>Pérez-Serrano, Rosa Martha</creator><creator>Camacho-Calderón, Nicolás</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8033-3520</orcidid><orcidid>https://orcid.org/0000-0003-2045-8951</orcidid><orcidid>https://orcid.org/0000-0003-0238-1559</orcidid></search><sort><creationdate>20221113</creationdate><title>Isothiocyanate-Rich Extracts from Cauliflower ( Brassica oleracea Var. Botrytis) and Radish ( Raphanus sativus ) Inhibited Metabolic Activity and Induced ROS in Selected Human HCT116 and HT-29 Colorectal Cancer Cells</title><author>Cuellar-Nuñez, Mardey Liceth ; Luzardo-Ocampo, Ivan ; Lee-Martínez, Sarah ; Larrauri-Rodríguez, Michelle ; Zaldívar-Lelo de Larrea, Guadalupe ; Pérez-Serrano, Rosa Martha ; Camacho-Calderón, Nicolás</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-41a94bc3a10deaabfad8f4126baee3c84ecbf42d100d4c4e70040de10f8b9bdd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adenocarcinoma</topic><topic>Allyl isothiocyanate</topic><topic>Apoptosis</topic><topic>Bioavailability</topic><topic>Biocompatibility</topic><topic>Botrytis</topic><topic>Brassica</topic><topic>Cauliflowers</topic><topic>Cell cycle</topic><topic>Colon</topic><topic>Colon cancer</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Colorectal Neoplasms - drug therapy</topic><topic>Cytotoxicity</topic><topic>DNA methylation</topic><topic>Fluorescence</topic><topic>Humans</topic><topic>Isothiocyanate</topic><topic>Isothiocyanates - pharmacology</topic><topic>Metabolism</topic><topic>NF-κB protein</topic><topic>Radishes</topic><topic>Raphanus</topic><topic>Reactive Oxygen Species</topic><topic>Vegetables</topic><topic>β-Catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cuellar-Nuñez, Mardey Liceth</creatorcontrib><creatorcontrib>Luzardo-Ocampo, Ivan</creatorcontrib><creatorcontrib>Lee-Martínez, Sarah</creatorcontrib><creatorcontrib>Larrauri-Rodríguez, Michelle</creatorcontrib><creatorcontrib>Zaldívar-Lelo de Larrea, Guadalupe</creatorcontrib><creatorcontrib>Pérez-Serrano, Rosa Martha</creatorcontrib><creatorcontrib>Camacho-Calderón, Nicolás</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of environmental research and public health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cuellar-Nuñez, Mardey Liceth</au><au>Luzardo-Ocampo, Ivan</au><au>Lee-Martínez, Sarah</au><au>Larrauri-Rodríguez, Michelle</au><au>Zaldívar-Lelo de Larrea, Guadalupe</au><au>Pérez-Serrano, Rosa Martha</au><au>Camacho-Calderón, Nicolás</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isothiocyanate-Rich Extracts from Cauliflower ( Brassica oleracea Var. Botrytis) and Radish ( Raphanus sativus ) Inhibited Metabolic Activity and Induced ROS in Selected Human HCT116 and HT-29 Colorectal Cancer Cells</atitle><jtitle>International journal of environmental research and public health</jtitle><addtitle>Int J Environ Res Public Health</addtitle><date>2022-11-13</date><risdate>2022</risdate><volume>19</volume><issue>22</issue><spage>14919</spage><pages>14919-</pages><issn>1660-4601</issn><issn>1661-7827</issn><eissn>1660-4601</eissn><abstract>Cruciferous vegetables such as cauliflower and radish contain isothiocyanates exhibiting chemoprotective effects in vitro and in vivo. This research aimed to assess the impact of cauliflower (CIE) and radish (RIE) isothiocyanate extracts on the metabolic activity, intracellular reactive oxygen species (ROS), and LDH production of selected human colorectal adenocarcinoma cells (HCT116 and HT-29 for early and late colon cancer development, respectively). Non-cancerous colon cells (CCD-33Co) were used as a cytotoxicity control. The CIE samples displayed the highest allyl isothiocyanate (AITC: 12.55 µg/g) contents, whereas RIE was the most abundant in benzyl isothiocyanate (BITC: 15.35 µg/g). Both extracts effectively inhibited HCT116 and HT-29 metabolic activity, but the CIE impact was higher than that of RIE on HCT116 (IC
: 0.56 mg/mL). Assays using the half-inhibitory concentrations (IC
) of all treatments, including AITC and BITC, displayed increased (
< 0.05) LDH (absorbance: 0.25-0.40 nm) and ROS release (1190-1697 relative fluorescence units) in both cell lines. BITC showed the highest in silico binding affinity with all the tested colorectal cancer molecular markers (NF-kB, β-catenin, and NRF2-NFE2). The theoretical evaluation of AITC and BITC bioavailability showed high values for both compounds. The results indicate that CIE and RIE extracts display chemopreventive effects in vitro, but additional experiments are needed to validate their effects.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>36429638</pmid><doi>10.3390/ijerph192214919</doi><orcidid>https://orcid.org/0000-0002-8033-3520</orcidid><orcidid>https://orcid.org/0000-0003-2045-8951</orcidid><orcidid>https://orcid.org/0000-0003-0238-1559</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of environmental research and public health, 2022-11, Vol.19 (22), p.14919 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adenocarcinoma Allyl isothiocyanate Apoptosis Bioavailability Biocompatibility Botrytis Brassica Cauliflowers Cell cycle Colon Colon cancer Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - drug therapy Cytotoxicity DNA methylation Fluorescence Humans Isothiocyanate Isothiocyanates - pharmacology Metabolism NF-κB protein Radishes Raphanus Reactive Oxygen Species Vegetables β-Catenin |
| title | Isothiocyanate-Rich Extracts from Cauliflower ( Brassica oleracea Var. Botrytis) and Radish ( Raphanus sativus ) Inhibited Metabolic Activity and Induced ROS in Selected Human HCT116 and HT-29 Colorectal Cancer Cells |
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