A solution to the biophysical fractionation of extracellular vesicles: Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER)
High-precision isolation of small extracellular vesicles (sEVs) from biofluids is essential toward developing next-generation liquid biopsies and regenerative therapies. However, current methods of sEV separation require specialized equipment and time-consuming protocols and have difficulties produc...
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| Veröffentlicht in: | Science advances 2022-11, Vol.8 (47), p.eade0640-eade0640 |
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| creator | Zhang, Jinxin Chen, Chuyi Becker, Ryan Rufo, Joseph Yang, Shujie Mai, John Zhang, Peiran Gu, Yuyang Wang, Zeyu Ma, Zhehan Xia, Jianping Hao, Nanjing Tian, Zhenhua Wong, David T W Sadovsky, Yoel Lee, Luke P Huang, Tony Jun |
| description | High-precision isolation of small extracellular vesicles (sEVs) from biofluids is essential toward developing next-generation liquid biopsies and regenerative therapies. However, current methods of sEV separation require specialized equipment and time-consuming protocols and have difficulties producing highly pure subpopulations of sEVs. Here, we present Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER), which allows single-step, rapid (96% small exosomes, >80% exomeres) fractionation of sEV subpopulations from biofluids without the need for any sample preprocessing. Particles are iteratively deflected in a size-selective manner via an excitation resonance. This previously unidentified phenomenon generates patterns of virtual, tunable, pillar-like acoustic field in a fluid using surface acoustic waves. Highly precise sEV fractionation without the need for sample preprocessing or complex nanofabrication methods has been demonstrated using ANSWER, showing potential as a powerful tool that will enable more in-depth studies into the complexity, heterogeneity, and functionality of sEV subpopulations. |
| doi_str_mv | 10.1126/sciadv.ade0640 |
| format | Article |
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However, current methods of sEV separation require specialized equipment and time-consuming protocols and have difficulties producing highly pure subpopulations of sEVs. Here, we present Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER), which allows single-step, rapid (<10 min), high-purity (>96% small exosomes, >80% exomeres) fractionation of sEV subpopulations from biofluids without the need for any sample preprocessing. Particles are iteratively deflected in a size-selective manner via an excitation resonance. This previously unidentified phenomenon generates patterns of virtual, tunable, pillar-like acoustic field in a fluid using surface acoustic waves. Highly precise sEV fractionation without the need for sample preprocessing or complex nanofabrication methods has been demonstrated using ANSWER, showing potential as a powerful tool that will enable more in-depth studies into the complexity, heterogeneity, and functionality of sEV subpopulations.</description><identifier>ISSN: 2375-2548</identifier><identifier>EISSN: 2375-2548</identifier><identifier>DOI: 10.1126/sciadv.ade0640</identifier><identifier>PMID: 36417505</identifier><language>eng</language><publisher>United States: American Association for the Advancement of Science</publisher><subject>Applied Physics ; Applied Sciences and Engineering ; Physical and Materials Sciences ; SciAdv r-articles</subject><ispartof>Science advances, 2022-11, Vol.8 (47), p.eade0640-eade0640</ispartof><rights>Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. 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However, current methods of sEV separation require specialized equipment and time-consuming protocols and have difficulties producing highly pure subpopulations of sEVs. Here, we present Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER), which allows single-step, rapid (<10 min), high-purity (>96% small exosomes, >80% exomeres) fractionation of sEV subpopulations from biofluids without the need for any sample preprocessing. Particles are iteratively deflected in a size-selective manner via an excitation resonance. This previously unidentified phenomenon generates patterns of virtual, tunable, pillar-like acoustic field in a fluid using surface acoustic waves. 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Chen, Chuyi ; Becker, Ryan ; Rufo, Joseph ; Yang, Shujie ; Mai, John ; Zhang, Peiran ; Gu, Yuyang ; Wang, Zeyu ; Ma, Zhehan ; Xia, Jianping ; Hao, Nanjing ; Tian, Zhenhua ; Wong, David T W ; Sadovsky, Yoel ; Lee, Luke P ; Huang, Tony Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-e1d7cc3c74f57a72e57af0876d35931a1ce82bbfa111524aa6f9203fef12321d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Applied Physics</topic><topic>Applied Sciences and Engineering</topic><topic>Physical and Materials Sciences</topic><topic>SciAdv r-articles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Jinxin</creatorcontrib><creatorcontrib>Chen, Chuyi</creatorcontrib><creatorcontrib>Becker, Ryan</creatorcontrib><creatorcontrib>Rufo, Joseph</creatorcontrib><creatorcontrib>Yang, Shujie</creatorcontrib><creatorcontrib>Mai, John</creatorcontrib><creatorcontrib>Zhang, Peiran</creatorcontrib><creatorcontrib>Gu, Yuyang</creatorcontrib><creatorcontrib>Wang, Zeyu</creatorcontrib><creatorcontrib>Ma, Zhehan</creatorcontrib><creatorcontrib>Xia, Jianping</creatorcontrib><creatorcontrib>Hao, Nanjing</creatorcontrib><creatorcontrib>Tian, Zhenhua</creatorcontrib><creatorcontrib>Wong, David T W</creatorcontrib><creatorcontrib>Sadovsky, Yoel</creatorcontrib><creatorcontrib>Lee, Luke P</creatorcontrib><creatorcontrib>Huang, Tony Jun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Science advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Jinxin</au><au>Chen, Chuyi</au><au>Becker, Ryan</au><au>Rufo, Joseph</au><au>Yang, Shujie</au><au>Mai, John</au><au>Zhang, Peiran</au><au>Gu, Yuyang</au><au>Wang, Zeyu</au><au>Ma, Zhehan</au><au>Xia, Jianping</au><au>Hao, Nanjing</au><au>Tian, Zhenhua</au><au>Wong, David T W</au><au>Sadovsky, Yoel</au><au>Lee, Luke P</au><au>Huang, Tony Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A solution to the biophysical fractionation of extracellular vesicles: Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER)</atitle><jtitle>Science advances</jtitle><addtitle>Sci Adv</addtitle><date>2022-11-25</date><risdate>2022</risdate><volume>8</volume><issue>47</issue><spage>eade0640</spage><epage>eade0640</epage><pages>eade0640-eade0640</pages><issn>2375-2548</issn><eissn>2375-2548</eissn><abstract>High-precision isolation of small extracellular vesicles (sEVs) from biofluids is essential toward developing next-generation liquid biopsies and regenerative therapies. However, current methods of sEV separation require specialized equipment and time-consuming protocols and have difficulties producing highly pure subpopulations of sEVs. Here, we present Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER), which allows single-step, rapid (<10 min), high-purity (>96% small exosomes, >80% exomeres) fractionation of sEV subpopulations from biofluids without the need for any sample preprocessing. Particles are iteratively deflected in a size-selective manner via an excitation resonance. This previously unidentified phenomenon generates patterns of virtual, tunable, pillar-like acoustic field in a fluid using surface acoustic waves. 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| subjects | Applied Physics Applied Sciences and Engineering Physical and Materials Sciences SciAdv r-articles |
| title | A solution to the biophysical fractionation of extracellular vesicles: Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER) |
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