Mechanism of PDZK1 in Hepatocellular Carcinoma Complicated with Hyperuricemia
Background. Hepatocellular carcinoma (HCC) is a kind of primary liver cancer that accounts for more than 90% of primary hepatocellular carcinomas. Hyperuricemia is closely related to the development, recurrence, metastasis, and prognosis of cancer. Previous studies have proved that the serum uric ac...
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| description | Background. Hepatocellular carcinoma (HCC) is a kind of primary liver cancer that accounts for more than 90% of primary hepatocellular carcinomas. Hyperuricemia is closely related to the development, recurrence, metastasis, and prognosis of cancer. Previous studies have proved that the serum uric acid level can increase the incidence rate and mortality of malignant tumors. However, the specific pathogenesis remains unstudied. Methods. RT-qPCR analysis showed that the mRNA expression of PDZK1 and ABCG2 increased significantly after HCC cells were exposed to different concentrations of soluble uric acid (2.5, 5, 10, 20 mg/dl) for 24 hours. Then, in HCC shRNAs, PDZK1, or over expression PDZK1 were used. CCK8, wound healing, and Transwell assay showed that PDZK1 regulates cell proliferation, invasion, and migration. Flow cytometry results revealed that PDZK1 affects cell apoptosis. Western blot results show that PDZK1 affects the STAT3/C-myc pathway. Then, in vivo tumorigenesis, allopurinol maybe an effective drug to advance: the prognosis of HCC. Results. In our study, RT-qPCR analysis showed that the mRNA expression of PDZK1 and ABCG2 increased significantly after different concentrations of soluble uric acid in HCC. Then, PDZK1 affects the proliferation, migration, and apoptosis of HCC through the STAT3/C-myc pathway. Conclusions. Hyperuricemia response affects the expression of PDZK1; PDZK1 affects the proliferation, migration, and apoptosis through the STAT3/C-myc pathway in hepatocellular carcinoma. It is suggested that PDZK1 maybe closely related to the occurrence, development, and prognosis of HCC and allopurinol maybe have potential anticancer effects. |
| doi_str_mv | 10.1155/2022/1403454 |
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| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9678461</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A728081614</galeid><sourcerecordid>A728081614</sourcerecordid><originalsourceid>FETCH-LOGICAL-c476t-67e80cf10c0a7dcd9520b86384e02db9d4e01c455b782f72ef27381ea6eda64b3</originalsourceid><addsrcrecordid>eNp9kUFv1DAQRi0EoqVw44wicUGiS23HsZ0LUrVAt2orOMCFizVxJl1XiR3shKr_Hke7lMKB01jy0xt__gh5yeg7xqrqhFPOT5igpajEI3LIpFYrLSr6-MH5gDxL6YZSKWgtn5KDUgpOy0ofkqsrtFvwLg1F6IovH75fsML5YoMjTMFi3889xGIN0TofBijWYRh7Z2HCtrh107bY3I0Y5-gsDg6ekycd9Alf7OcR-fbp49f1ZnX5-ex8fXq5skLJaSUVamo7Ri0F1dq2rjhttCy1QMrbpm7zZFZUVaM07xTHjqtSMwSJLUjRlEfk_c47zs2ArUU_RejNGN0A8c4EcObvG--25jr8NLVUWkiWBW_2ghh-zJgmM7i0xAWPYU4m76uV4JKJjL7-B70Jc_Q5XqbE8ouSsj_UNfRonO9C3msXqTlVXFPNdq7jHWVjSClid_9kRs3SplnaNPs2M_7qYcx7-Hd9GXi7A7bOt3Dr_q_7BccopYU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2740358601</pqid></control><display><type>article</type><title>Mechanism of PDZK1 in Hepatocellular Carcinoma Complicated with Hyperuricemia</title><source>Wiley Online Library Open Access</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>PubMed Central Open Access</source><creator>Guo, Linqi ; Jiang, Wenda ; Quan, Lingli ; Teng, Xinli ; Zhao, Jing ; Qiu, Hongbin</creator><contributor>Muddassir Ali, Muhammad</contributor><creatorcontrib>Guo, Linqi ; Jiang, Wenda ; Quan, Lingli ; Teng, Xinli ; Zhao, Jing ; Qiu, Hongbin ; Muddassir Ali, Muhammad</creatorcontrib><description>Background. Hepatocellular carcinoma (HCC) is a kind of primary liver cancer that accounts for more than 90% of primary hepatocellular carcinomas. Hyperuricemia is closely related to the development, recurrence, metastasis, and prognosis of cancer. Previous studies have proved that the serum uric acid level can increase the incidence rate and mortality of malignant tumors. However, the specific pathogenesis remains unstudied. Methods. RT-qPCR analysis showed that the mRNA expression of PDZK1 and ABCG2 increased significantly after HCC cells were exposed to different concentrations of soluble uric acid (2.5, 5, 10, 20 mg/dl) for 24 hours. Then, in HCC shRNAs, PDZK1, or over expression PDZK1 were used. CCK8, wound healing, and Transwell assay showed that PDZK1 regulates cell proliferation, invasion, and migration. Flow cytometry results revealed that PDZK1 affects cell apoptosis. Western blot results show that PDZK1 affects the STAT3/C-myc pathway. Then, in vivo tumorigenesis, allopurinol maybe an effective drug to advance: the prognosis of HCC. Results. In our study, RT-qPCR analysis showed that the mRNA expression of PDZK1 and ABCG2 increased significantly after different concentrations of soluble uric acid in HCC. Then, PDZK1 affects the proliferation, migration, and apoptosis of HCC through the STAT3/C-myc pathway. Conclusions. Hyperuricemia response affects the expression of PDZK1; PDZK1 affects the proliferation, migration, and apoptosis through the STAT3/C-myc pathway in hepatocellular carcinoma. It is suggested that PDZK1 maybe closely related to the occurrence, development, and prognosis of HCC and allopurinol maybe have potential anticancer effects.</description><identifier>ISSN: 1687-8450</identifier><identifier>EISSN: 1687-8450</identifier><identifier>DOI: 10.1155/2022/1403454</identifier><identifier>PMID: 36420358</identifier><language>eng</language><publisher>Egypt: Hindawi</publisher><subject>Amino acids ; Analysis ; Apoptosis ; Care and treatment ; Flow cytometry ; Gout ; Hepatitis ; Hepatoma ; Hyperuricemia ; Liver cancer ; Liver diseases ; Medical prognosis ; Pathogenesis ; Prognosis ; Prostate ; Proteins ; RNA ; Software ; Statistical analysis ; Tumors ; Uric acid ; Wound healing</subject><ispartof>Journal of oncology, 2022-11, Vol.2022, p.1403454-10</ispartof><rights>Copyright © 2022 Linqi Guo et al.</rights><rights>COPYRIGHT 2022 John Wiley & Sons, Inc.</rights><rights>Copyright © 2022 Linqi Guo et al. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2022 Linqi Guo et al. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-67e80cf10c0a7dcd9520b86384e02db9d4e01c455b782f72ef27381ea6eda64b3</citedby><cites>FETCH-LOGICAL-c476t-67e80cf10c0a7dcd9520b86384e02db9d4e01c455b782f72ef27381ea6eda64b3</cites><orcidid>0000-0002-9547-6743</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678461/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9678461/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36420358$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Muddassir Ali, Muhammad</contributor><creatorcontrib>Guo, Linqi</creatorcontrib><creatorcontrib>Jiang, Wenda</creatorcontrib><creatorcontrib>Quan, Lingli</creatorcontrib><creatorcontrib>Teng, Xinli</creatorcontrib><creatorcontrib>Zhao, Jing</creatorcontrib><creatorcontrib>Qiu, Hongbin</creatorcontrib><title>Mechanism of PDZK1 in Hepatocellular Carcinoma Complicated with Hyperuricemia</title><title>Journal of oncology</title><addtitle>J Oncol</addtitle><description>Background. Hepatocellular carcinoma (HCC) is a kind of primary liver cancer that accounts for more than 90% of primary hepatocellular carcinomas. Hyperuricemia is closely related to the development, recurrence, metastasis, and prognosis of cancer. Previous studies have proved that the serum uric acid level can increase the incidence rate and mortality of malignant tumors. However, the specific pathogenesis remains unstudied. Methods. RT-qPCR analysis showed that the mRNA expression of PDZK1 and ABCG2 increased significantly after HCC cells were exposed to different concentrations of soluble uric acid (2.5, 5, 10, 20 mg/dl) for 24 hours. Then, in HCC shRNAs, PDZK1, or over expression PDZK1 were used. CCK8, wound healing, and Transwell assay showed that PDZK1 regulates cell proliferation, invasion, and migration. Flow cytometry results revealed that PDZK1 affects cell apoptosis. Western blot results show that PDZK1 affects the STAT3/C-myc pathway. Then, in vivo tumorigenesis, allopurinol maybe an effective drug to advance: the prognosis of HCC. Results. In our study, RT-qPCR analysis showed that the mRNA expression of PDZK1 and ABCG2 increased significantly after different concentrations of soluble uric acid in HCC. Then, PDZK1 affects the proliferation, migration, and apoptosis of HCC through the STAT3/C-myc pathway. Conclusions. Hyperuricemia response affects the expression of PDZK1; PDZK1 affects the proliferation, migration, and apoptosis through the STAT3/C-myc pathway in hepatocellular carcinoma. It is suggested that PDZK1 maybe closely related to the occurrence, development, and prognosis of HCC and allopurinol maybe have potential anticancer effects.</description><subject>Amino acids</subject><subject>Analysis</subject><subject>Apoptosis</subject><subject>Care and treatment</subject><subject>Flow cytometry</subject><subject>Gout</subject><subject>Hepatitis</subject><subject>Hepatoma</subject><subject>Hyperuricemia</subject><subject>Liver cancer</subject><subject>Liver diseases</subject><subject>Medical prognosis</subject><subject>Pathogenesis</subject><subject>Prognosis</subject><subject>Prostate</subject><subject>Proteins</subject><subject>RNA</subject><subject>Software</subject><subject>Statistical analysis</subject><subject>Tumors</subject><subject>Uric acid</subject><subject>Wound healing</subject><issn>1687-8450</issn><issn>1687-8450</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUFv1DAQRi0EoqVw44wicUGiS23HsZ0LUrVAt2orOMCFizVxJl1XiR3shKr_Hke7lMKB01jy0xt__gh5yeg7xqrqhFPOT5igpajEI3LIpFYrLSr6-MH5gDxL6YZSKWgtn5KDUgpOy0ofkqsrtFvwLg1F6IovH75fsML5YoMjTMFi3889xGIN0TofBijWYRh7Z2HCtrh107bY3I0Y5-gsDg6ekycd9Alf7OcR-fbp49f1ZnX5-ex8fXq5skLJaSUVamo7Ri0F1dq2rjhttCy1QMrbpm7zZFZUVaM07xTHjqtSMwSJLUjRlEfk_c47zs2ArUU_RejNGN0A8c4EcObvG--25jr8NLVUWkiWBW_2ghh-zJgmM7i0xAWPYU4m76uV4JKJjL7-B70Jc_Q5XqbE8ouSsj_UNfRonO9C3msXqTlVXFPNdq7jHWVjSClid_9kRs3SplnaNPs2M_7qYcx7-Hd9GXi7A7bOt3Dr_q_7BccopYU</recordid><startdate>20221114</startdate><enddate>20221114</enddate><creator>Guo, Linqi</creator><creator>Jiang, Wenda</creator><creator>Quan, Lingli</creator><creator>Teng, Xinli</creator><creator>Zhao, Jing</creator><creator>Qiu, Hongbin</creator><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9547-6743</orcidid></search><sort><creationdate>20221114</creationdate><title>Mechanism of PDZK1 in Hepatocellular Carcinoma Complicated with Hyperuricemia</title><author>Guo, Linqi ; Jiang, Wenda ; Quan, Lingli ; Teng, Xinli ; Zhao, Jing ; Qiu, Hongbin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-67e80cf10c0a7dcd9520b86384e02db9d4e01c455b782f72ef27381ea6eda64b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Amino acids</topic><topic>Analysis</topic><topic>Apoptosis</topic><topic>Care and treatment</topic><topic>Flow cytometry</topic><topic>Gout</topic><topic>Hepatitis</topic><topic>Hepatoma</topic><topic>Hyperuricemia</topic><topic>Liver cancer</topic><topic>Liver diseases</topic><topic>Medical prognosis</topic><topic>Pathogenesis</topic><topic>Prognosis</topic><topic>Prostate</topic><topic>Proteins</topic><topic>RNA</topic><topic>Software</topic><topic>Statistical analysis</topic><topic>Tumors</topic><topic>Uric acid</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Linqi</creatorcontrib><creatorcontrib>Jiang, Wenda</creatorcontrib><creatorcontrib>Quan, Lingli</creatorcontrib><creatorcontrib>Teng, Xinli</creatorcontrib><creatorcontrib>Zhao, Jing</creatorcontrib><creatorcontrib>Qiu, Hongbin</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Linqi</au><au>Jiang, Wenda</au><au>Quan, Lingli</au><au>Teng, Xinli</au><au>Zhao, Jing</au><au>Qiu, Hongbin</au><au>Muddassir Ali, Muhammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism of PDZK1 in Hepatocellular Carcinoma Complicated with Hyperuricemia</atitle><jtitle>Journal of oncology</jtitle><addtitle>J Oncol</addtitle><date>2022-11-14</date><risdate>2022</risdate><volume>2022</volume><spage>1403454</spage><epage>10</epage><pages>1403454-10</pages><issn>1687-8450</issn><eissn>1687-8450</eissn><abstract>Background. Hepatocellular carcinoma (HCC) is a kind of primary liver cancer that accounts for more than 90% of primary hepatocellular carcinomas. Hyperuricemia is closely related to the development, recurrence, metastasis, and prognosis of cancer. Previous studies have proved that the serum uric acid level can increase the incidence rate and mortality of malignant tumors. However, the specific pathogenesis remains unstudied. Methods. RT-qPCR analysis showed that the mRNA expression of PDZK1 and ABCG2 increased significantly after HCC cells were exposed to different concentrations of soluble uric acid (2.5, 5, 10, 20 mg/dl) for 24 hours. Then, in HCC shRNAs, PDZK1, or over expression PDZK1 were used. CCK8, wound healing, and Transwell assay showed that PDZK1 regulates cell proliferation, invasion, and migration. Flow cytometry results revealed that PDZK1 affects cell apoptosis. Western blot results show that PDZK1 affects the STAT3/C-myc pathway. Then, in vivo tumorigenesis, allopurinol maybe an effective drug to advance: the prognosis of HCC. Results. In our study, RT-qPCR analysis showed that the mRNA expression of PDZK1 and ABCG2 increased significantly after different concentrations of soluble uric acid in HCC. Then, PDZK1 affects the proliferation, migration, and apoptosis of HCC through the STAT3/C-myc pathway. Conclusions. Hyperuricemia response affects the expression of PDZK1; PDZK1 affects the proliferation, migration, and apoptosis through the STAT3/C-myc pathway in hepatocellular carcinoma. It is suggested that PDZK1 maybe closely related to the occurrence, development, and prognosis of HCC and allopurinol maybe have potential anticancer effects.</abstract><cop>Egypt</cop><pub>Hindawi</pub><pmid>36420358</pmid><doi>10.1155/2022/1403454</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-9547-6743</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Amino acids Analysis Apoptosis Care and treatment Flow cytometry Gout Hepatitis Hepatoma Hyperuricemia Liver cancer Liver diseases Medical prognosis Pathogenesis Prognosis Prostate Proteins RNA Software Statistical analysis Tumors Uric acid Wound healing |
| title | Mechanism of PDZK1 in Hepatocellular Carcinoma Complicated with Hyperuricemia |
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