7β-22 Dihydroxyhopane, Isolated from the Sub-Antarctic Lichen, Inhibits the Viability and Stemness in Glioma Stem Like Cells
Background: Glioma stem cells (GSCs) have been reported to contribute to tumor initiation and relapse, therapy resistance, and intra-tumoral heterogeneity of glioblastoma multiforme. Therefore, inhibiting GSCs presents a critical therapeutic tactic to suppress the aggressiveness of tumors. Methods:...
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| Veröffentlicht in: | OncoTargets and therapy 2022, Vol.15, p.1375-1383 |
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| creator | Kim, Hyun-Jin Suh, Sung-Suk Park, Jeongwon Shin, Min-Ji Koo, Man Hyung Lee, Suk Jun Jeon, Young-Jun Lee, Seongsoo Youn, Ui-Joung Kim, Sung-Hak |
| description | Background: Glioma stem cells (GSCs) have been reported to contribute to tumor initiation and relapse, therapy resistance, and intra-tumoral heterogeneity of glioblastoma multiforme. Therefore, inhibiting GSCs presents a critical therapeutic tactic to suppress the aggressiveness of tumors. Methods: In this study, we examined the effects of 7β-22 dihydroxyhopane (AP 18), isolated from the sub-Antarctic lichen, Pseudocyphellaria freycinetii. The cytotoxic effect of AP 18 and its effects on cell proliferation were assessed by alamarBlue assay and 5-ethynyl-2′-deoxyuridine (EdU) assay. Real-time confluence analysis was performed with a Celloger automatic live cell imaging system. Western Blotting and 3-D optical diffraction tomography (ODT) imaging were performed to determine whether apoptosis was triggered by AP 18. A Limiting dilution assay and qRT-PCR were performed to investigate the impact of AP 18 on GSC stemness. Results: AP 18 significantly reduced GSCs viability and proliferation, inducing programmed cell death identified by Annexin V/PI staining and had effects on morphologic features determined by 3-D ODT. Interestingly, treatment with AP 18 suppressed stemness features. Conclusion: Taken together, our results suggest that AP 18 might be a potential therapeutic agent to target GSCs. |
| doi_str_mv | 10.2147/OTT.S371042 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9675426</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2737136459</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3012-5c25f1ba95e45cc505a76a2b217196841e43cdad1a7a383a7b2370c2523bc8a13</originalsourceid><addsrcrecordid>eNpdkUtO5DAQhiPESDyGFRewxAYJAn4lTjZIqJkBpJZYdMPWqjhuYkjsxnbQ9IJLcZA5EwZaiJlVlaq--lVVf5btE3xCCRenN_P5yYwJgjndyLYJEVVe1gxvfsu3sp0QHjAuy4ry7exF_H3NKUUXplu13v1ZdW4JVh-j6-B6iLpFC-8GFDuNZmOTn9sIXkWj0NSoTtvE2c40JoYP5M5AY3oTVwhsi2ZRD1aHgIxFl71xA3yU0uijRhPd9-Fn9mMBfdB767ib3f7-NZ9c5dOby-vJ-TRXDBOaF4oWC9JAXWheKFXgAkQJtKFEkLqsONGcqRZaAgJYxUA0lAmchihrVAWE7WZnn7rLsRl0q7SNHnq59GYAv5IOjPy3Y00n792zrEtRcFomgcO1gHdPow5RDiaodEL6lRuDpILV6aW84Ak9-A99cKO36bx3ShCWqDpRR5-U8i4ErxdfyxAs372UyUu59pK9Aeeckl8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2737136459</pqid></control><display><type>article</type><title>7β-22 Dihydroxyhopane, Isolated from the Sub-Antarctic Lichen, Inhibits the Viability and Stemness in Glioma Stem Like Cells</title><source>Dove Press Free</source><source>PubMed Central Open Access</source><source>Access via Taylor & Francis (Open Access Collection)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Kim, Hyun-Jin ; Suh, Sung-Suk ; Park, Jeongwon ; Shin, Min-Ji ; Koo, Man Hyung ; Lee, Suk Jun ; Jeon, Young-Jun ; Lee, Seongsoo ; Youn, Ui-Joung ; Kim, Sung-Hak</creator><creatorcontrib>Kim, Hyun-Jin ; Suh, Sung-Suk ; Park, Jeongwon ; Shin, Min-Ji ; Koo, Man Hyung ; Lee, Suk Jun ; Jeon, Young-Jun ; Lee, Seongsoo ; Youn, Ui-Joung ; Kim, Sung-Hak</creatorcontrib><description>Background: Glioma stem cells (GSCs) have been reported to contribute to tumor initiation and relapse, therapy resistance, and intra-tumoral heterogeneity of glioblastoma multiforme. Therefore, inhibiting GSCs presents a critical therapeutic tactic to suppress the aggressiveness of tumors. Methods: In this study, we examined the effects of 7β-22 dihydroxyhopane (AP 18), isolated from the sub-Antarctic lichen, Pseudocyphellaria freycinetii. The cytotoxic effect of AP 18 and its effects on cell proliferation were assessed by alamarBlue assay and 5-ethynyl-2′-deoxyuridine (EdU) assay. Real-time confluence analysis was performed with a Celloger automatic live cell imaging system. Western Blotting and 3-D optical diffraction tomography (ODT) imaging were performed to determine whether apoptosis was triggered by AP 18. A Limiting dilution assay and qRT-PCR were performed to investigate the impact of AP 18 on GSC stemness. Results: AP 18 significantly reduced GSCs viability and proliferation, inducing programmed cell death identified by Annexin V/PI staining and had effects on morphologic features determined by 3-D ODT. Interestingly, treatment with AP 18 suppressed stemness features. Conclusion: Taken together, our results suggest that AP 18 might be a potential therapeutic agent to target GSCs.</description><identifier>ISSN: 1178-6930</identifier><identifier>EISSN: 1178-6930</identifier><identifier>DOI: 10.2147/OTT.S371042</identifier><language>eng</language><publisher>Macclesfield: Taylor & Francis Ltd</publisher><subject>Acids ; Annexin V ; Apoptosis ; Brain cancer ; Brain research ; Brain tumors ; Cancer therapies ; Cell culture ; Cell death ; Cell proliferation ; Chemotherapy ; Cytotoxicity ; Epidermal growth factor ; Glioblastoma ; Glioma ; Glioma cells ; Laboratories ; Lichens ; Original Research ; R&D ; Research & development ; Solvents ; Stem cells ; Western blotting</subject><ispartof>OncoTargets and therapy, 2022, Vol.15, p.1375-1383</ispartof><rights>2022. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 Kim et al. 2022 Kim et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3012-5c25f1ba95e45cc505a76a2b217196841e43cdad1a7a383a7b2370c2523bc8a13</citedby><cites>FETCH-LOGICAL-c3012-5c25f1ba95e45cc505a76a2b217196841e43cdad1a7a383a7b2370c2523bc8a13</cites><orcidid>0000-0001-9338-7934 ; 0000-0002-3217-1010</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9675426/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9675426/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3862,4024,27923,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Kim, Hyun-Jin</creatorcontrib><creatorcontrib>Suh, Sung-Suk</creatorcontrib><creatorcontrib>Park, Jeongwon</creatorcontrib><creatorcontrib>Shin, Min-Ji</creatorcontrib><creatorcontrib>Koo, Man Hyung</creatorcontrib><creatorcontrib>Lee, Suk Jun</creatorcontrib><creatorcontrib>Jeon, Young-Jun</creatorcontrib><creatorcontrib>Lee, Seongsoo</creatorcontrib><creatorcontrib>Youn, Ui-Joung</creatorcontrib><creatorcontrib>Kim, Sung-Hak</creatorcontrib><title>7β-22 Dihydroxyhopane, Isolated from the Sub-Antarctic Lichen, Inhibits the Viability and Stemness in Glioma Stem Like Cells</title><title>OncoTargets and therapy</title><description>Background: Glioma stem cells (GSCs) have been reported to contribute to tumor initiation and relapse, therapy resistance, and intra-tumoral heterogeneity of glioblastoma multiforme. Therefore, inhibiting GSCs presents a critical therapeutic tactic to suppress the aggressiveness of tumors. Methods: In this study, we examined the effects of 7β-22 dihydroxyhopane (AP 18), isolated from the sub-Antarctic lichen, Pseudocyphellaria freycinetii. The cytotoxic effect of AP 18 and its effects on cell proliferation were assessed by alamarBlue assay and 5-ethynyl-2′-deoxyuridine (EdU) assay. Real-time confluence analysis was performed with a Celloger automatic live cell imaging system. Western Blotting and 3-D optical diffraction tomography (ODT) imaging were performed to determine whether apoptosis was triggered by AP 18. A Limiting dilution assay and qRT-PCR were performed to investigate the impact of AP 18 on GSC stemness. Results: AP 18 significantly reduced GSCs viability and proliferation, inducing programmed cell death identified by Annexin V/PI staining and had effects on morphologic features determined by 3-D ODT. Interestingly, treatment with AP 18 suppressed stemness features. Conclusion: Taken together, our results suggest that AP 18 might be a potential therapeutic agent to target GSCs.</description><subject>Acids</subject><subject>Annexin V</subject><subject>Apoptosis</subject><subject>Brain cancer</subject><subject>Brain research</subject><subject>Brain tumors</subject><subject>Cancer therapies</subject><subject>Cell culture</subject><subject>Cell death</subject><subject>Cell proliferation</subject><subject>Chemotherapy</subject><subject>Cytotoxicity</subject><subject>Epidermal growth factor</subject><subject>Glioblastoma</subject><subject>Glioma</subject><subject>Glioma cells</subject><subject>Laboratories</subject><subject>Lichens</subject><subject>Original Research</subject><subject>R&D</subject><subject>Research & development</subject><subject>Solvents</subject><subject>Stem cells</subject><subject>Western blotting</subject><issn>1178-6930</issn><issn>1178-6930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkUtO5DAQhiPESDyGFRewxAYJAn4lTjZIqJkBpJZYdMPWqjhuYkjsxnbQ9IJLcZA5EwZaiJlVlaq--lVVf5btE3xCCRenN_P5yYwJgjndyLYJEVVe1gxvfsu3sp0QHjAuy4ry7exF_H3NKUUXplu13v1ZdW4JVh-j6-B6iLpFC-8GFDuNZmOTn9sIXkWj0NSoTtvE2c40JoYP5M5AY3oTVwhsi2ZRD1aHgIxFl71xA3yU0uijRhPd9-Fn9mMBfdB767ib3f7-NZ9c5dOby-vJ-TRXDBOaF4oWC9JAXWheKFXgAkQJtKFEkLqsONGcqRZaAgJYxUA0lAmchihrVAWE7WZnn7rLsRl0q7SNHnq59GYAv5IOjPy3Y00n792zrEtRcFomgcO1gHdPow5RDiaodEL6lRuDpILV6aW84Ak9-A99cKO36bx3ShCWqDpRR5-U8i4ErxdfyxAs372UyUu59pK9Aeeckl8</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Kim, Hyun-Jin</creator><creator>Suh, Sung-Suk</creator><creator>Park, Jeongwon</creator><creator>Shin, Min-Ji</creator><creator>Koo, Man Hyung</creator><creator>Lee, Suk Jun</creator><creator>Jeon, Young-Jun</creator><creator>Lee, Seongsoo</creator><creator>Youn, Ui-Joung</creator><creator>Kim, Sung-Hak</creator><general>Taylor & Francis Ltd</general><general>Dove</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9338-7934</orcidid><orcidid>https://orcid.org/0000-0002-3217-1010</orcidid></search><sort><creationdate>2022</creationdate><title>7β-22 Dihydroxyhopane, Isolated from the Sub-Antarctic Lichen, Inhibits the Viability and Stemness in Glioma Stem Like Cells</title><author>Kim, Hyun-Jin ; Suh, Sung-Suk ; Park, Jeongwon ; Shin, Min-Ji ; Koo, Man Hyung ; Lee, Suk Jun ; Jeon, Young-Jun ; Lee, Seongsoo ; Youn, Ui-Joung ; Kim, Sung-Hak</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3012-5c25f1ba95e45cc505a76a2b217196841e43cdad1a7a383a7b2370c2523bc8a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acids</topic><topic>Annexin V</topic><topic>Apoptosis</topic><topic>Brain cancer</topic><topic>Brain research</topic><topic>Brain tumors</topic><topic>Cancer therapies</topic><topic>Cell culture</topic><topic>Cell death</topic><topic>Cell proliferation</topic><topic>Chemotherapy</topic><topic>Cytotoxicity</topic><topic>Epidermal growth factor</topic><topic>Glioblastoma</topic><topic>Glioma</topic><topic>Glioma cells</topic><topic>Laboratories</topic><topic>Lichens</topic><topic>Original Research</topic><topic>R&D</topic><topic>Research & development</topic><topic>Solvents</topic><topic>Stem cells</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hyun-Jin</creatorcontrib><creatorcontrib>Suh, Sung-Suk</creatorcontrib><creatorcontrib>Park, Jeongwon</creatorcontrib><creatorcontrib>Shin, Min-Ji</creatorcontrib><creatorcontrib>Koo, Man Hyung</creatorcontrib><creatorcontrib>Lee, Suk Jun</creatorcontrib><creatorcontrib>Jeon, Young-Jun</creatorcontrib><creatorcontrib>Lee, Seongsoo</creatorcontrib><creatorcontrib>Youn, Ui-Joung</creatorcontrib><creatorcontrib>Kim, Sung-Hak</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>OncoTargets and therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hyun-Jin</au><au>Suh, Sung-Suk</au><au>Park, Jeongwon</au><au>Shin, Min-Ji</au><au>Koo, Man Hyung</au><au>Lee, Suk Jun</au><au>Jeon, Young-Jun</au><au>Lee, Seongsoo</au><au>Youn, Ui-Joung</au><au>Kim, Sung-Hak</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>7β-22 Dihydroxyhopane, Isolated from the Sub-Antarctic Lichen, Inhibits the Viability and Stemness in Glioma Stem Like Cells</atitle><jtitle>OncoTargets and therapy</jtitle><date>2022</date><risdate>2022</risdate><volume>15</volume><spage>1375</spage><epage>1383</epage><pages>1375-1383</pages><issn>1178-6930</issn><eissn>1178-6930</eissn><abstract>Background: Glioma stem cells (GSCs) have been reported to contribute to tumor initiation and relapse, therapy resistance, and intra-tumoral heterogeneity of glioblastoma multiforme. Therefore, inhibiting GSCs presents a critical therapeutic tactic to suppress the aggressiveness of tumors. Methods: In this study, we examined the effects of 7β-22 dihydroxyhopane (AP 18), isolated from the sub-Antarctic lichen, Pseudocyphellaria freycinetii. The cytotoxic effect of AP 18 and its effects on cell proliferation were assessed by alamarBlue assay and 5-ethynyl-2′-deoxyuridine (EdU) assay. Real-time confluence analysis was performed with a Celloger automatic live cell imaging system. Western Blotting and 3-D optical diffraction tomography (ODT) imaging were performed to determine whether apoptosis was triggered by AP 18. A Limiting dilution assay and qRT-PCR were performed to investigate the impact of AP 18 on GSC stemness. Results: AP 18 significantly reduced GSCs viability and proliferation, inducing programmed cell death identified by Annexin V/PI staining and had effects on morphologic features determined by 3-D ODT. Interestingly, treatment with AP 18 suppressed stemness features. Conclusion: Taken together, our results suggest that AP 18 might be a potential therapeutic agent to target GSCs.</abstract><cop>Macclesfield</cop><pub>Taylor & Francis Ltd</pub><doi>10.2147/OTT.S371042</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-9338-7934</orcidid><orcidid>https://orcid.org/0000-0002-3217-1010</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Annexin V Apoptosis Brain cancer Brain research Brain tumors Cancer therapies Cell culture Cell death Cell proliferation Chemotherapy Cytotoxicity Epidermal growth factor Glioblastoma Glioma Glioma cells Laboratories Lichens Original Research R&D Research & development Solvents Stem cells Western blotting |
| title | 7β-22 Dihydroxyhopane, Isolated from the Sub-Antarctic Lichen, Inhibits the Viability and Stemness in Glioma Stem Like Cells |
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