Effects of vimentin on the migration, search efficiency, and mechanical resilience of dendritic cells

Dendritic cells use amoeboid migration to pass through narrow passages in the extracellular matrix and confined tissue in search for pathogens and to reach the lymph nodes and alert the immune system. Amoeboid migration is a migration mode that, instead of relying on cell adhesion, is based on mecha...

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Veröffentlicht in:Biophysical journal 2022-10, Vol.121 (20), p.3950-3961
Hauptverfasser: Shaebani, M. Reza, Stankevicins, Luiza, Vesperini, Doriane, Urbanska, Marta, Flormann, Daniel A.D., Terriac, Emmanuel, Gad, Annica K.B., Cheng, Fang, Eriksson, John E., Lautenschläger, Franziska
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container_end_page 3961
container_issue 20
container_start_page 3950
container_title Biophysical journal
container_volume 121
creator Shaebani, M. Reza
Stankevicins, Luiza
Vesperini, Doriane
Urbanska, Marta
Flormann, Daniel A.D.
Terriac, Emmanuel
Gad, Annica K.B.
Cheng, Fang
Eriksson, John E.
Lautenschläger, Franziska
description Dendritic cells use amoeboid migration to pass through narrow passages in the extracellular matrix and confined tissue in search for pathogens and to reach the lymph nodes and alert the immune system. Amoeboid migration is a migration mode that, instead of relying on cell adhesion, is based on mechanical resilience and friction. To better understand the role of intermediate filaments in ameboid migration, we studied the effects of vimentin on the migration of dendritic cells. We show that the lymph node homing of vimentin-deficient cells is reduced in our in vivo experiments in mice. Lack of vimentin also reduces the cell stiffness, the number of migrating cells, and the migration speed in vitro in both 1D and 2D confined environments. Moreover, we find that lack of vimentin weakens the correlation between directional persistence and migration speed. Thus, vimentin-expressing dendritic cells move faster in straighter lines. Our numerical simulations of persistent random search in confined geometries verify that the reduced migration speed and the weaker correlation between the speed and direction of motion result in longer search times to find regularly located targets. Together, these observations show that vimentin enhances the ameboid migration of dendritic cells, which is relevant for the efficiency of their random search for pathogens.
doi_str_mv 10.1016/j.bpj.2022.08.033
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source MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects Amoeba
Animals
Cell Adhesion
Cell Movement
Dendritic Cells - metabolism
Intermediate Filaments - metabolism
Mice
Vimentin
title Effects of vimentin on the migration, search efficiency, and mechanical resilience of dendritic cells
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