Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes

Cells in complex organisms undergo frequent functional changes, but few methods allow comprehensive longitudinal profiling of living cells. Here we introduce scission-accelerated fluorophore exchange (SAFE), a method for multiplexed temporospatial imaging of living cells with immunofluorescence. SAF...

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Veröffentlicht in:	Nature biotechnology 2022-11, Vol.40 (11), p.1654-1662
Hauptverfasser:	Ko, Jina, Wilkovitsch, Martin, Oh, Juhyun, Kohler, Rainer H., Bolli, Evangelia, Pittet, Mikael J., Vinegoni, Claudio, Sykes, David B., Mikula, Hannes, Weissleder, Ralph, Carlson, Jonathan C. T.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/245/2225 631/61/32 639/638/549/2260 639/638/92/96 Agriculture Animals Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Fluorescent Antibody Technique Fluorescent Dyes - chemistry Leukocytes, Mononuclear Life Sciences Mice Optical Imaging - methods Staining and Labeling
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