Complementary Regulation of BfmRS Two-Component and AbaIR Quorum Sensing Systems to Express Virulence-Associated Genes in Acinetobacter baumannii
Acinetobacter baumannii expresses various virulence factors to adapt to hostile environments and infect susceptible hosts. This study investigated the regulatory network of the BfmRS two-component and AbaIR quorum sensing (QS) systems in the expression of virulence-associated genes in A. baumannii A...
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| Veröffentlicht in: | International journal of molecular sciences 2022-11, Vol.23 (21), p.13136 |
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| creator | Kim, Hyo-Jeong Kim, Na-Yeong Ko, Seo-Yeon Park, Seong-Yong Oh, Man-Hwan Shin, Min-Sang Lee, Yoo-Chul Lee, Je-Chul |
| description | Acinetobacter baumannii expresses various virulence factors to adapt to hostile environments and infect susceptible hosts. This study investigated the regulatory network of the BfmRS two-component and AbaIR quorum sensing (QS) systems in the expression of virulence-associated genes in A. baumannii ATCC 17978. The ΔbfmS mutant exhibited a significant decrease in surface motility, which presumably resulted from the low expression of pilT and A1S_0112-A1S_0119 gene cluster. The ΔbfmR mutant displayed a significant reduction in biofilm and pellicle formation due to the low expression of csu operon. The deletion of abaR did not affect the expression of bfmR or bfmS. However, the expression of abaR and abaI was upregulated in the ΔbfmR mutant. The ΔbfmR mutant also produced more autoinducers than did the wild-type strain, suggesting that BfmR negatively regulates the AbaIR QS system. The ΔbfmS mutant exhibited no autoinducer production in the bioassay system. The expression of the A1S_0112-A1S_0119 gene cluster was downregulated in the ΔabaR mutant, whereas the expression of csu operon was upregulated in this mutant with a high cell density. In conclusion, for the first time, we demonstrated that the BfmRS-AbaIR QS system axis regulated the expression of virulence-associated genes in A. baumannii. This study provides new insights into the complex network system involved in the regulation of virulence-associated genes underlying the pathogenicity of A. baumannii. |
| doi_str_mv | 10.3390/ijms232113136 |
| format | Article |
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This study investigated the regulatory network of the BfmRS two-component and AbaIR quorum sensing (QS) systems in the expression of virulence-associated genes in A. baumannii ATCC 17978. The ΔbfmS mutant exhibited a significant decrease in surface motility, which presumably resulted from the low expression of pilT and A1S_0112-A1S_0119 gene cluster. The ΔbfmR mutant displayed a significant reduction in biofilm and pellicle formation due to the low expression of csu operon. The deletion of abaR did not affect the expression of bfmR or bfmS. However, the expression of abaR and abaI was upregulated in the ΔbfmR mutant. The ΔbfmR mutant also produced more autoinducers than did the wild-type strain, suggesting that BfmR negatively regulates the AbaIR QS system. The ΔbfmS mutant exhibited no autoinducer production in the bioassay system. The expression of the A1S_0112-A1S_0119 gene cluster was downregulated in the ΔabaR mutant, whereas the expression of csu operon was upregulated in this mutant with a high cell density. In conclusion, for the first time, we demonstrated that the BfmRS-AbaIR QS system axis regulated the expression of virulence-associated genes in A. baumannii. This study provides new insights into the complex network system involved in the regulation of virulence-associated genes underlying the pathogenicity of A. baumannii.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms232113136</identifier><identifier>PMID: 36361923</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Acinetobacter baumannii ; Antimicrobial agents ; Biofilms ; Cell density ; Gene expression ; Gene regulation ; Genes ; Kinases ; Motility ; Mutants ; Pathogenicity ; Pathogens ; Pellicle ; Physiology ; Quorum sensing ; Virulence ; Virulence factors</subject><ispartof>International journal of molecular sciences, 2022-11, Vol.23 (21), p.13136</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-677de7a83b18e6f275db45a3e3edc64535b46571c7a5c63d4b51ebb2856f39ca3</citedby><cites>FETCH-LOGICAL-c392t-677de7a83b18e6f275db45a3e3edc64535b46571c7a5c63d4b51ebb2856f39ca3</cites><orcidid>0000-0001-8679-446X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657202/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657202/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids></links><search><creatorcontrib>Kim, Hyo-Jeong</creatorcontrib><creatorcontrib>Kim, Na-Yeong</creatorcontrib><creatorcontrib>Ko, Seo-Yeon</creatorcontrib><creatorcontrib>Park, Seong-Yong</creatorcontrib><creatorcontrib>Oh, Man-Hwan</creatorcontrib><creatorcontrib>Shin, Min-Sang</creatorcontrib><creatorcontrib>Lee, Yoo-Chul</creatorcontrib><creatorcontrib>Lee, Je-Chul</creatorcontrib><title>Complementary Regulation of BfmRS Two-Component and AbaIR Quorum Sensing Systems to Express Virulence-Associated Genes in Acinetobacter baumannii</title><title>International journal of molecular sciences</title><description>Acinetobacter baumannii expresses various virulence factors to adapt to hostile environments and infect susceptible hosts. This study investigated the regulatory network of the BfmRS two-component and AbaIR quorum sensing (QS) systems in the expression of virulence-associated genes in A. baumannii ATCC 17978. The ΔbfmS mutant exhibited a significant decrease in surface motility, which presumably resulted from the low expression of pilT and A1S_0112-A1S_0119 gene cluster. The ΔbfmR mutant displayed a significant reduction in biofilm and pellicle formation due to the low expression of csu operon. The deletion of abaR did not affect the expression of bfmR or bfmS. However, the expression of abaR and abaI was upregulated in the ΔbfmR mutant. The ΔbfmR mutant also produced more autoinducers than did the wild-type strain, suggesting that BfmR negatively regulates the AbaIR QS system. The ΔbfmS mutant exhibited no autoinducer production in the bioassay system. The expression of the A1S_0112-A1S_0119 gene cluster was downregulated in the ΔabaR mutant, whereas the expression of csu operon was upregulated in this mutant with a high cell density. In conclusion, for the first time, we demonstrated that the BfmRS-AbaIR QS system axis regulated the expression of virulence-associated genes in A. baumannii. This study provides new insights into the complex network system involved in the regulation of virulence-associated genes underlying the pathogenicity of A. baumannii.</description><subject>Acinetobacter baumannii</subject><subject>Antimicrobial agents</subject><subject>Biofilms</subject><subject>Cell density</subject><subject>Gene expression</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Kinases</subject><subject>Motility</subject><subject>Mutants</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Pellicle</subject><subject>Physiology</subject><subject>Quorum sensing</subject><subject>Virulence</subject><subject>Virulence factors</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkUFv1DAQhS0EomXhyN0SFy6B2BM7yQVpWZVSqRJit3C1bGeyeBXbi-0A_Rn8Y7JqhSinGWm-eZr3hpCXrH4D0Ndv3cFnDpwxYCAfkXPWcF7VtWwf_9OfkWc5H-p6AUX_lJyBBMl6Dufk9yb644QeQ9Hplm5xP0-6uBhoHOn70W939OZnrE5UDAtEdRjo2uirLf08xzR7usOQXdjT3W0u6DMtkV78OibMmX51aZ4wWKzWOUfrdMGBXmLATF2ga-sClmi0LZio0bPXITj3nDwZ9ZTxxX1dkS8fLm42H6vrT5dXm_V1ZaHnpZJtO2CrOzCsQznyVgymERoQcLCyESBMI0XLbKuFlTA0RjA0hndCjtBbDSvy7k73OBu_7Czmkp7UMTm_JKGidurhJLhvah9_qH6R5UuUK_L6XiDF7zPmorzLFqdJB4xzVrwF0UnJ6mZBX_2HHuKcwmLvRDUSul6cqOqOsinmnHD8ewyr1enZ6sGz4Q94CJ62</recordid><startdate>20221101</startdate><enddate>20221101</enddate><creator>Kim, Hyo-Jeong</creator><creator>Kim, Na-Yeong</creator><creator>Ko, Seo-Yeon</creator><creator>Park, Seong-Yong</creator><creator>Oh, Man-Hwan</creator><creator>Shin, Min-Sang</creator><creator>Lee, Yoo-Chul</creator><creator>Lee, Je-Chul</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8679-446X</orcidid></search><sort><creationdate>20221101</creationdate><title>Complementary Regulation of BfmRS Two-Component and AbaIR Quorum Sensing Systems to Express Virulence-Associated Genes in Acinetobacter baumannii</title><author>Kim, Hyo-Jeong ; 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This study investigated the regulatory network of the BfmRS two-component and AbaIR quorum sensing (QS) systems in the expression of virulence-associated genes in A. baumannii ATCC 17978. The ΔbfmS mutant exhibited a significant decrease in surface motility, which presumably resulted from the low expression of pilT and A1S_0112-A1S_0119 gene cluster. The ΔbfmR mutant displayed a significant reduction in biofilm and pellicle formation due to the low expression of csu operon. The deletion of abaR did not affect the expression of bfmR or bfmS. However, the expression of abaR and abaI was upregulated in the ΔbfmR mutant. The ΔbfmR mutant also produced more autoinducers than did the wild-type strain, suggesting that BfmR negatively regulates the AbaIR QS system. The ΔbfmS mutant exhibited no autoinducer production in the bioassay system. The expression of the A1S_0112-A1S_0119 gene cluster was downregulated in the ΔabaR mutant, whereas the expression of csu operon was upregulated in this mutant with a high cell density. In conclusion, for the first time, we demonstrated that the BfmRS-AbaIR QS system axis regulated the expression of virulence-associated genes in A. baumannii. This study provides new insights into the complex network system involved in the regulation of virulence-associated genes underlying the pathogenicity of A. baumannii.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>36361923</pmid><doi>10.3390/ijms232113136</doi><orcidid>https://orcid.org/0000-0001-8679-446X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acinetobacter baumannii Antimicrobial agents Biofilms Cell density Gene expression Gene regulation Genes Kinases Motility Mutants Pathogenicity Pathogens Pellicle Physiology Quorum sensing Virulence Virulence factors |
| title | Complementary Regulation of BfmRS Two-Component and AbaIR Quorum Sensing Systems to Express Virulence-Associated Genes in Acinetobacter baumannii |
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