Surface Modification of PHBV Fibrous Scaffold via Lithium Borohydride Reduction

In this study, lithium borohydride (LiBH4) reduction was used to modify the surface chemistry of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) fibers. Although the most common reaction employed in the surface treatment of polyester materials is hydrolysis, it is not suitable for fiber modifica...

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Veröffentlicht in:Materials 2022-10, Vol.15 (21), p.7494
Hauptverfasser: Chaber, Paweł, Tylko, Grzegorz, Włodarczyk, Jakub, Nitschke, Paweł, Hercog, Anna, Jurczyk, Sebastian, Rech, Jakub, Kubacki, Jerzy, Adamus, Grażyna
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container_issue 21
container_start_page 7494
container_title Materials
container_volume 15
creator Chaber, Paweł
Tylko, Grzegorz
Włodarczyk, Jakub
Nitschke, Paweł
Hercog, Anna
Jurczyk, Sebastian
Rech, Jakub
Kubacki, Jerzy
Adamus, Grażyna
description In this study, lithium borohydride (LiBH4) reduction was used to modify the surface chemistry of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) fibers. Although the most common reaction employed in the surface treatment of polyester materials is hydrolysis, it is not suitable for fiber modification of bacterial polyesters, which are highly resistant to this type of reaction. The use of LiBH4 allowed the formation of surface hydroxyl groups under very mild conditions, which was crucial for maintaining the fibers’ integrity. The presence of these groups resulted in a noticeable improvement in the surface hydrophilicity of PHBV, as revealed by contact angle measurements. After the treatment with a LiBH4 solution, the electrospun PHBV fibrous mat had a significantly greater number of viable osteoblast-like cells (SaOS-2 cell line) than the untreated mat. Moreover, the results of the cell proliferation measurements correlated well with the observed cell morphology. The most flattened SaOS-2 cells were found on the surface that supported the best cell attachment. Most importantly, the results of our study indicated that the degree of surface modification could be controlled by changing the degradation time and concentration of the borohydride solution. This was of great importance since it allowed optimization of the surface properties to achieve the highest cell-proliferation capacity.
doi_str_mv 10.3390/ma15217494
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source MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; PubMed Central Open Access
subjects Biodegradation
Borohydrides
Cell adhesion & migration
Contact angle
Hydroxyl groups
Lithium
Morphology
Optimization
Polyester resins
Polymerization
Polymers
Radiation
Reduction
Surface chemistry
Surface properties
Surface treatment
Tissue engineering
title Surface Modification of PHBV Fibrous Scaffold via Lithium Borohydride Reduction
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