Surface Modification of PHBV Fibrous Scaffold via Lithium Borohydride Reduction
In this study, lithium borohydride (LiBH4) reduction was used to modify the surface chemistry of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) fibers. Although the most common reaction employed in the surface treatment of polyester materials is hydrolysis, it is not suitable for fiber modifica...
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creator | Chaber, Paweł Tylko, Grzegorz Włodarczyk, Jakub Nitschke, Paweł Hercog, Anna Jurczyk, Sebastian Rech, Jakub Kubacki, Jerzy Adamus, Grażyna |
description | In this study, lithium borohydride (LiBH4) reduction was used to modify the surface chemistry of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) fibers. Although the most common reaction employed in the surface treatment of polyester materials is hydrolysis, it is not suitable for fiber modification of bacterial polyesters, which are highly resistant to this type of reaction. The use of LiBH4 allowed the formation of surface hydroxyl groups under very mild conditions, which was crucial for maintaining the fibers’ integrity. The presence of these groups resulted in a noticeable improvement in the surface hydrophilicity of PHBV, as revealed by contact angle measurements. After the treatment with a LiBH4 solution, the electrospun PHBV fibrous mat had a significantly greater number of viable osteoblast-like cells (SaOS-2 cell line) than the untreated mat. Moreover, the results of the cell proliferation measurements correlated well with the observed cell morphology. The most flattened SaOS-2 cells were found on the surface that supported the best cell attachment. Most importantly, the results of our study indicated that the degree of surface modification could be controlled by changing the degradation time and concentration of the borohydride solution. This was of great importance since it allowed optimization of the surface properties to achieve the highest cell-proliferation capacity. |
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Although the most common reaction employed in the surface treatment of polyester materials is hydrolysis, it is not suitable for fiber modification of bacterial polyesters, which are highly resistant to this type of reaction. The use of LiBH4 allowed the formation of surface hydroxyl groups under very mild conditions, which was crucial for maintaining the fibers’ integrity. The presence of these groups resulted in a noticeable improvement in the surface hydrophilicity of PHBV, as revealed by contact angle measurements. After the treatment with a LiBH4 solution, the electrospun PHBV fibrous mat had a significantly greater number of viable osteoblast-like cells (SaOS-2 cell line) than the untreated mat. Moreover, the results of the cell proliferation measurements correlated well with the observed cell morphology. The most flattened SaOS-2 cells were found on the surface that supported the best cell attachment. Most importantly, the results of our study indicated that the degree of surface modification could be controlled by changing the degradation time and concentration of the borohydride solution. This was of great importance since it allowed optimization of the surface properties to achieve the highest cell-proliferation capacity.</description><identifier>ISSN: 1996-1944</identifier><identifier>EISSN: 1996-1944</identifier><identifier>DOI: 10.3390/ma15217494</identifier><identifier>PMID: 36363086</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Biodegradation ; Borohydrides ; Cell adhesion & migration ; Contact angle ; Hydroxyl groups ; Lithium ; Morphology ; Optimization ; Polyester resins ; Polymerization ; Polymers ; Radiation ; Reduction ; Surface chemistry ; Surface properties ; Surface treatment ; Tissue engineering</subject><ispartof>Materials, 2022-10, Vol.15 (21), p.7494</ispartof><rights>COPYRIGHT 2022 MDPI AG</rights><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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subjects | Biodegradation Borohydrides Cell adhesion & migration Contact angle Hydroxyl groups Lithium Morphology Optimization Polyester resins Polymerization Polymers Radiation Reduction Surface chemistry Surface properties Surface treatment Tissue engineering |
title | Surface Modification of PHBV Fibrous Scaffold via Lithium Borohydride Reduction |
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