Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence
Aeromonas veronii is a pathogen which can induce diseases in humans, animals and aquatic organisms, but its pathogenic mechanism and virulence factors are still elusive. In this study, we successfully constructed a mutant strain (Δ ascP ) by homologous recombination. The results showed that the dele...
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| Veröffentlicht in: | The journal of microbiology 2022-12, Vol.60 (12), p.1153-1161 |
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| creator | Guan, Yongchao Zhang, Meng Wang, Yingda Liu, Zhongzhuo Zhao, Zelin Wang, Hong An, Dingjie Qian, Aidong Kang, Yuanhuan Sun, Wuwen Shan, Xiaofeng |
| description | Aeromonas veronii
is a pathogen which can induce diseases in humans, animals and aquatic organisms, but its pathogenic mechanism and virulence factors are still elusive. In this study, we successfully constructed a mutant strain (Δ
ascP
) by homologous recombination. The results showed that the deletion of the
ascP
gene significantly down-regulated the expression of associated effector proteins in
A. veronii
compared to its wild type. The adhesive and invasive abilities of Δ
ascP
to EPC cells were 0.82-fold lower in contrast to the wild strain. The toxicity of Δ
ascP
to cells was decreased by about 2.91-fold (1 h) and 1.74-fold (2 h). Furthermore, the LD
50
of the mutant strain of
crucian carp
was reduced by 19.94-fold, and the virulence was considerably attenuated. In contrast to the wild strain, the Δ
ascP
content in the liver and spleen was considerably lower. The titers of serum cytokines (IL-8, TNF-α, and IL-1β) in
crucian carp
after the infection of the Δ
ascP
strain were considerably lower in contrast to the wild strain. Hence, the
ascP
gene is essential for the etiopathogenesis of
A. veronii
TH0426. |
| doi_str_mv | 10.1007/s12275-022-2373-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9647756</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2739763664</sourcerecordid><originalsourceid>FETCH-LOGICAL-c447t-7d8fe0dd3c5e37638834f09806d55ea41381b527684e686381d1be533fbeb0f03</originalsourceid><addsrcrecordid>eNp1kV9LHDEUxUNp6dq1H6BvAV_6MjX_k3kpiGgVBH3Q55CZubNmnU22yczCfvtmutKq4Ety4fzO4XIPQt8o-UEJ0aeZMqZlRRirGNe8Mh_QEa21qriuxccX8wJ9yXlNiKJcsM9owRWXkun6CPnLKbSjj8EN2JVnn33Gsccut3fYB3wGKW6KmvGuTMF7fH9FBFM4wQ7ckLHDT7DHKQ4w4-MjFGU1DW7OnIN2Pk0DhBaO0ae-GODr879ED5cX9-dX1c3tr-vzs5uqFUKPle5MD6TreCuBa8WN4aIntSGqkxKcoNzQpuyujABlik472oDkvG-gIT3hS_TzkLudmg10LYQxucFuk9-4tLfReftaCf7RruLO1kpoLVUJ-P4ckOLvCfJoNz63MAwuQJyyZZpLqgkltKAnb9B1nFI541-qXJ8rJQpFD1SbYs4J-n_LUGLnIu2hSFuKtHOR1hQPO3hyYcMK0v_k901_AO8Nns8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2739763664</pqid></control><display><type>article</type><title>Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence</title><source>Springer Nature</source><creator>Guan, Yongchao ; Zhang, Meng ; Wang, Yingda ; Liu, Zhongzhuo ; Zhao, Zelin ; Wang, Hong ; An, Dingjie ; Qian, Aidong ; Kang, Yuanhuan ; Sun, Wuwen ; Shan, Xiaofeng</creator><creatorcontrib>Guan, Yongchao ; Zhang, Meng ; Wang, Yingda ; Liu, Zhongzhuo ; Zhao, Zelin ; Wang, Hong ; An, Dingjie ; Qian, Aidong ; Kang, Yuanhuan ; Sun, Wuwen ; Shan, Xiaofeng</creatorcontrib><description>Aeromonas veronii
is a pathogen which can induce diseases in humans, animals and aquatic organisms, but its pathogenic mechanism and virulence factors are still elusive. In this study, we successfully constructed a mutant strain (Δ
ascP
) by homologous recombination. The results showed that the deletion of the
ascP
gene significantly down-regulated the expression of associated effector proteins in
A. veronii
compared to its wild type. The adhesive and invasive abilities of Δ
ascP
to EPC cells were 0.82-fold lower in contrast to the wild strain. The toxicity of Δ
ascP
to cells was decreased by about 2.91-fold (1 h) and 1.74-fold (2 h). Furthermore, the LD
50
of the mutant strain of
crucian carp
was reduced by 19.94-fold, and the virulence was considerably attenuated. In contrast to the wild strain, the Δ
ascP
content in the liver and spleen was considerably lower. The titers of serum cytokines (IL-8, TNF-α, and IL-1β) in
crucian carp
after the infection of the Δ
ascP
strain were considerably lower in contrast to the wild strain. Hence, the
ascP
gene is essential for the etiopathogenesis of
A. veronii
TH0426.</description><identifier>ISSN: 1976-3794</identifier><identifier>ISSN: 1225-8873</identifier><identifier>EISSN: 1976-3794</identifier><identifier>DOI: 10.1007/s12275-022-2373-8</identifier><identifier>PMID: 36355279</identifier><language>eng</language><publisher>Seoul: The Microbiological Society of Korea</publisher><subject>Aeromonas veronii ; Animal diseases ; Aquatic animals ; Aquatic organisms ; Biomedical and Life Sciences ; Carp ; Cells ; Cytokines ; Functional analysis ; Gene deletion ; Gene expression ; Genomics and Molecular Biology ; Homologous recombination ; IL-1β ; Interleukin 8 ; Life Sciences ; Microbial Genetics ; Microbiology ; Mortality causes ; Mutants ; Pathogens ; Recombination ; Serum ; Single cell proteins ; Spleen ; Toxicity ; Toxicity tests ; Tumor necrosis factor-α ; Virulence ; Virulence factors</subject><ispartof>The journal of microbiology, 2022-12, Vol.60 (12), p.1153-1161</ispartof><rights>Author(s) 2022. under the exclusive license with the Microbiological Society of Korea</rights><rights>Author(s) 2022. under the exclusive license with the Microbiological Society of Korea.</rights><rights>Author(s) 2022, under the exclusive license with the Microbiological Society of Korea</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-7d8fe0dd3c5e37638834f09806d55ea41381b527684e686381d1be533fbeb0f03</citedby><cites>FETCH-LOGICAL-c447t-7d8fe0dd3c5e37638834f09806d55ea41381b527684e686381d1be533fbeb0f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12275-022-2373-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12275-022-2373-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Guan, Yongchao</creatorcontrib><creatorcontrib>Zhang, Meng</creatorcontrib><creatorcontrib>Wang, Yingda</creatorcontrib><creatorcontrib>Liu, Zhongzhuo</creatorcontrib><creatorcontrib>Zhao, Zelin</creatorcontrib><creatorcontrib>Wang, Hong</creatorcontrib><creatorcontrib>An, Dingjie</creatorcontrib><creatorcontrib>Qian, Aidong</creatorcontrib><creatorcontrib>Kang, Yuanhuan</creatorcontrib><creatorcontrib>Sun, Wuwen</creatorcontrib><creatorcontrib>Shan, Xiaofeng</creatorcontrib><title>Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence</title><title>The journal of microbiology</title><addtitle>J Microbiol</addtitle><description>Aeromonas veronii
is a pathogen which can induce diseases in humans, animals and aquatic organisms, but its pathogenic mechanism and virulence factors are still elusive. In this study, we successfully constructed a mutant strain (Δ
ascP
) by homologous recombination. The results showed that the deletion of the
ascP
gene significantly down-regulated the expression of associated effector proteins in
A. veronii
compared to its wild type. The adhesive and invasive abilities of Δ
ascP
to EPC cells were 0.82-fold lower in contrast to the wild strain. The toxicity of Δ
ascP
to cells was decreased by about 2.91-fold (1 h) and 1.74-fold (2 h). Furthermore, the LD
50
of the mutant strain of
crucian carp
was reduced by 19.94-fold, and the virulence was considerably attenuated. In contrast to the wild strain, the Δ
ascP
content in the liver and spleen was considerably lower. The titers of serum cytokines (IL-8, TNF-α, and IL-1β) in
crucian carp
after the infection of the Δ
ascP
strain were considerably lower in contrast to the wild strain. Hence, the
ascP
gene is essential for the etiopathogenesis of
A. veronii
TH0426.</description><subject>Aeromonas veronii</subject><subject>Animal diseases</subject><subject>Aquatic animals</subject><subject>Aquatic organisms</subject><subject>Biomedical and Life Sciences</subject><subject>Carp</subject><subject>Cells</subject><subject>Cytokines</subject><subject>Functional analysis</subject><subject>Gene deletion</subject><subject>Gene expression</subject><subject>Genomics and Molecular Biology</subject><subject>Homologous recombination</subject><subject>IL-1β</subject><subject>Interleukin 8</subject><subject>Life Sciences</subject><subject>Microbial Genetics</subject><subject>Microbiology</subject><subject>Mortality causes</subject><subject>Mutants</subject><subject>Pathogens</subject><subject>Recombination</subject><subject>Serum</subject><subject>Single cell proteins</subject><subject>Spleen</subject><subject>Toxicity</subject><subject>Toxicity tests</subject><subject>Tumor necrosis factor-α</subject><subject>Virulence</subject><subject>Virulence factors</subject><issn>1976-3794</issn><issn>1225-8873</issn><issn>1976-3794</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kV9LHDEUxUNp6dq1H6BvAV_6MjX_k3kpiGgVBH3Q55CZubNmnU22yczCfvtmutKq4Ety4fzO4XIPQt8o-UEJ0aeZMqZlRRirGNe8Mh_QEa21qriuxccX8wJ9yXlNiKJcsM9owRWXkun6CPnLKbSjj8EN2JVnn33Gsccut3fYB3wGKW6KmvGuTMF7fH9FBFM4wQ7ckLHDT7DHKQ4w4-MjFGU1DW7OnIN2Pk0DhBaO0ae-GODr879ED5cX9-dX1c3tr-vzs5uqFUKPle5MD6TreCuBa8WN4aIntSGqkxKcoNzQpuyujABlik472oDkvG-gIT3hS_TzkLudmg10LYQxucFuk9-4tLfReftaCf7RruLO1kpoLVUJ-P4ckOLvCfJoNz63MAwuQJyyZZpLqgkltKAnb9B1nFI541-qXJ8rJQpFD1SbYs4J-n_LUGLnIu2hSFuKtHOR1hQPO3hyYcMK0v_k901_AO8Nns8</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Guan, Yongchao</creator><creator>Zhang, Meng</creator><creator>Wang, Yingda</creator><creator>Liu, Zhongzhuo</creator><creator>Zhao, Zelin</creator><creator>Wang, Hong</creator><creator>An, Dingjie</creator><creator>Qian, Aidong</creator><creator>Kang, Yuanhuan</creator><creator>Sun, Wuwen</creator><creator>Shan, Xiaofeng</creator><general>The Microbiological Society of Korea</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TM</scope><scope>7TN</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>K9.</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20221201</creationdate><title>Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence</title><author>Guan, Yongchao ; Zhang, Meng ; Wang, Yingda ; Liu, Zhongzhuo ; Zhao, Zelin ; Wang, Hong ; An, Dingjie ; Qian, Aidong ; Kang, Yuanhuan ; Sun, Wuwen ; Shan, Xiaofeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-7d8fe0dd3c5e37638834f09806d55ea41381b527684e686381d1be533fbeb0f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aeromonas veronii</topic><topic>Animal diseases</topic><topic>Aquatic animals</topic><topic>Aquatic organisms</topic><topic>Biomedical and Life Sciences</topic><topic>Carp</topic><topic>Cells</topic><topic>Cytokines</topic><topic>Functional analysis</topic><topic>Gene deletion</topic><topic>Gene expression</topic><topic>Genomics and Molecular Biology</topic><topic>Homologous recombination</topic><topic>IL-1β</topic><topic>Interleukin 8</topic><topic>Life Sciences</topic><topic>Microbial Genetics</topic><topic>Microbiology</topic><topic>Mortality causes</topic><topic>Mutants</topic><topic>Pathogens</topic><topic>Recombination</topic><topic>Serum</topic><topic>Single cell proteins</topic><topic>Spleen</topic><topic>Toxicity</topic><topic>Toxicity tests</topic><topic>Tumor necrosis factor-α</topic><topic>Virulence</topic><topic>Virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guan, Yongchao</creatorcontrib><creatorcontrib>Zhang, Meng</creatorcontrib><creatorcontrib>Wang, Yingda</creatorcontrib><creatorcontrib>Liu, Zhongzhuo</creatorcontrib><creatorcontrib>Zhao, Zelin</creatorcontrib><creatorcontrib>Wang, Hong</creatorcontrib><creatorcontrib>An, Dingjie</creatorcontrib><creatorcontrib>Qian, Aidong</creatorcontrib><creatorcontrib>Kang, Yuanhuan</creatorcontrib><creatorcontrib>Sun, Wuwen</creatorcontrib><creatorcontrib>Shan, Xiaofeng</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guan, Yongchao</au><au>Zhang, Meng</au><au>Wang, Yingda</au><au>Liu, Zhongzhuo</au><au>Zhao, Zelin</au><au>Wang, Hong</au><au>An, Dingjie</au><au>Qian, Aidong</au><au>Kang, Yuanhuan</au><au>Sun, Wuwen</au><au>Shan, Xiaofeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence</atitle><jtitle>The journal of microbiology</jtitle><stitle>J Microbiol</stitle><date>2022-12-01</date><risdate>2022</risdate><volume>60</volume><issue>12</issue><spage>1153</spage><epage>1161</epage><pages>1153-1161</pages><issn>1976-3794</issn><issn>1225-8873</issn><eissn>1976-3794</eissn><abstract>Aeromonas veronii
is a pathogen which can induce diseases in humans, animals and aquatic organisms, but its pathogenic mechanism and virulence factors are still elusive. In this study, we successfully constructed a mutant strain (Δ
ascP
) by homologous recombination. The results showed that the deletion of the
ascP
gene significantly down-regulated the expression of associated effector proteins in
A. veronii
compared to its wild type. The adhesive and invasive abilities of Δ
ascP
to EPC cells were 0.82-fold lower in contrast to the wild strain. The toxicity of Δ
ascP
to cells was decreased by about 2.91-fold (1 h) and 1.74-fold (2 h). Furthermore, the LD
50
of the mutant strain of
crucian carp
was reduced by 19.94-fold, and the virulence was considerably attenuated. In contrast to the wild strain, the Δ
ascP
content in the liver and spleen was considerably lower. The titers of serum cytokines (IL-8, TNF-α, and IL-1β) in
crucian carp
after the infection of the Δ
ascP
strain were considerably lower in contrast to the wild strain. Hence, the
ascP
gene is essential for the etiopathogenesis of
A. veronii
TH0426.</abstract><cop>Seoul</cop><pub>The Microbiological Society of Korea</pub><pmid>36355279</pmid><doi>10.1007/s12275-022-2373-8</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1976-3794 |
| ispartof | The journal of microbiology, 2022-12, Vol.60 (12), p.1153-1161 |
| issn | 1976-3794 1225-8873 1976-3794 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9647756 |
| source | Springer Nature |
| subjects | Aeromonas veronii Animal diseases Aquatic animals Aquatic organisms Biomedical and Life Sciences Carp Cells Cytokines Functional analysis Gene deletion Gene expression Genomics and Molecular Biology Homologous recombination IL-1β Interleukin 8 Life Sciences Microbial Genetics Microbiology Mortality causes Mutants Pathogens Recombination Serum Single cell proteins Spleen Toxicity Toxicity tests Tumor necrosis factor-α Virulence Virulence factors |
| title | Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence |
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