Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus
To date, the miRNA expression profile of plasma exosomes in women whose pregnancy is complicated by gestational diabetes mellitus (GDM) has not been fully clarified. In this study, differentially expressed miRNAs in plasma exosomes were identified by high-throughput small-RNA sequencing in 12 pregna...
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| Veröffentlicht in: | Diabetes (New York, N.Y.) N.Y.), 2022-11, Vol.71 (11), p.2272-2283 |
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| creator | Ye, Zhixin Wang, Songzi Huang, Xiaoqing Chen, Peisong Deng, Langhui Li, Shiqi Lin, Suiwen Wang, Zilian Liu, Bin |
| description | To date, the miRNA expression profile of plasma exosomes in women whose pregnancy is complicated by gestational diabetes mellitus (GDM) has not been fully clarified. In this study, differentially expressed miRNAs in plasma exosomes were identified by high-throughput small-RNA sequencing in 12 pregnant women with GDM and 12 with normal glucose tolerance (NGT) and validated in 102 pregnant women with GDM and 101 with NGT. A total of 22 exosomal miRNAs were found, five of which were verified by real-time qPCR. Exosomal miR-423-5p was upregulated, whereas miR-122-5p, miR-148a-3p, miR-192-5p, and miR-99a-5p were downregulated in women whose pregnancy was complicated by GDM. IGF1R and GYS1 as target genes of miR-423-5p, and G6PC3 and FDFT1 as target genes of miR-122-5p were associated with insulin and AMPK signaling pathways and may participate in the regulation of metabolism in GDM. The five exosomal miRNAs had an area under the curve of 0.82 (95%CI, 0.73, ∼0.91) in early prediction of GDM. Our study demonstrates that dysregulated exosomal miRNAs in plasma from pregnant women with GDM might influence the insulin and AMPK signaling pathways and could contribute to the early prediction of GDM. |
| doi_str_mv | 10.2337/db21-0909 |
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In this study, differentially expressed miRNAs in plasma exosomes were identified by high-throughput small-RNA sequencing in 12 pregnant women with GDM and 12 with normal glucose tolerance (NGT) and validated in 102 pregnant women with GDM and 101 with NGT. A total of 22 exosomal miRNAs were found, five of which were verified by real-time qPCR. Exosomal miR-423-5p was upregulated, whereas miR-122-5p, miR-148a-3p, miR-192-5p, and miR-99a-5p were downregulated in women whose pregnancy was complicated by GDM. IGF1R and GYS1 as target genes of miR-423-5p, and G6PC3 and FDFT1 as target genes of miR-122-5p were associated with insulin and AMPK signaling pathways and may participate in the regulation of metabolism in GDM. The five exosomal miRNAs had an area under the curve of 0.82 (95%CI, 0.73, ∼0.91) in early prediction of GDM. Our study demonstrates that dysregulated exosomal miRNAs in plasma from pregnant women with GDM might influence the insulin and AMPK signaling pathways and could contribute to the early prediction of GDM.</description><identifier>ISSN: 0012-1797</identifier><identifier>EISSN: 1939-327X</identifier><identifier>DOI: 10.2337/db21-0909</identifier><identifier>PMID: 35926094</identifier><language>eng</language><publisher>United States: American Diabetes Association</publisher><subject>AMP-Activated Protein Kinases - metabolism ; Chromosome 5 ; Diabetes mellitus ; Diabetes, Gestational - metabolism ; Exosomes ; Exosomes - genetics ; Exosomes - metabolism ; Female ; Gestational diabetes ; Glucose - metabolism ; Glucose tolerance ; Humans ; Insulin ; Insulin - metabolism ; Metabolism ; MicroRNAs - metabolism ; miRNA ; Plasma ; Pregnancy ; Signal transduction</subject><ispartof>Diabetes (New York, N.Y.), 2022-11, Vol.71 (11), p.2272-2283</ispartof><rights>2022 by the American Diabetes Association.</rights><rights>Copyright American Diabetes Association Nov 2022</rights><rights>2022 by the American Diabetes Association 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-4cd9a625a7ae6e4989d3525fd8c07de06a5d29372e064315c53f1ce67caff0903</citedby><cites>FETCH-LOGICAL-c403t-4cd9a625a7ae6e4989d3525fd8c07de06a5d29372e064315c53f1ce67caff0903</cites><orcidid>0000-0003-0208-6066</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630082/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630082/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35926094$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ye, Zhixin</creatorcontrib><creatorcontrib>Wang, Songzi</creatorcontrib><creatorcontrib>Huang, Xiaoqing</creatorcontrib><creatorcontrib>Chen, Peisong</creatorcontrib><creatorcontrib>Deng, Langhui</creatorcontrib><creatorcontrib>Li, Shiqi</creatorcontrib><creatorcontrib>Lin, Suiwen</creatorcontrib><creatorcontrib>Wang, Zilian</creatorcontrib><creatorcontrib>Liu, Bin</creatorcontrib><title>Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus</title><title>Diabetes (New York, N.Y.)</title><addtitle>Diabetes</addtitle><description>To date, the miRNA expression profile of plasma exosomes in women whose pregnancy is complicated by gestational diabetes mellitus (GDM) has not been fully clarified. In this study, differentially expressed miRNAs in plasma exosomes were identified by high-throughput small-RNA sequencing in 12 pregnant women with GDM and 12 with normal glucose tolerance (NGT) and validated in 102 pregnant women with GDM and 101 with NGT. A total of 22 exosomal miRNAs were found, five of which were verified by real-time qPCR. Exosomal miR-423-5p was upregulated, whereas miR-122-5p, miR-148a-3p, miR-192-5p, and miR-99a-5p were downregulated in women whose pregnancy was complicated by GDM. IGF1R and GYS1 as target genes of miR-423-5p, and G6PC3 and FDFT1 as target genes of miR-122-5p were associated with insulin and AMPK signaling pathways and may participate in the regulation of metabolism in GDM. The five exosomal miRNAs had an area under the curve of 0.82 (95%CI, 0.73, ∼0.91) in early prediction of GDM. Our study demonstrates that dysregulated exosomal miRNAs in plasma from pregnant women with GDM might influence the insulin and AMPK signaling pathways and could contribute to the early prediction of GDM.</description><subject>AMP-Activated Protein Kinases - metabolism</subject><subject>Chromosome 5</subject><subject>Diabetes mellitus</subject><subject>Diabetes, Gestational - metabolism</subject><subject>Exosomes</subject><subject>Exosomes - genetics</subject><subject>Exosomes - metabolism</subject><subject>Female</subject><subject>Gestational diabetes</subject><subject>Glucose - metabolism</subject><subject>Glucose tolerance</subject><subject>Humans</subject><subject>Insulin</subject><subject>Insulin - metabolism</subject><subject>Metabolism</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>Plasma</subject><subject>Pregnancy</subject><subject>Signal transduction</subject><issn>0012-1797</issn><issn>1939-327X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkdFqFDEUhoModlu98AUk4I29mHqSzEw2N8LSrrVQtYiid-FskrEpM5uakyn27c3SWlRykcD58nF-fsZeCDiSSuk3fiNFAwbMI7YQRplGSf39MVsACNkIbfQe2ye6AoC-nqdsT3VG9mDaBQsXI9KEfP0rUZpw5FP8_HFFfEWUXMQSPP8WyyX_EApu0hhp4kh8jXm85Rc5-OhKyjwN_DRQwRLTtjpOIm5CCVR_jWMsMz1jTwYcKTy_vw_Y13frL8fvm_NPp2fHq_PGtaBK0zpvsJcdagx9aM3SeNXJbvBLB9oH6LHz0igt67NVonOdGoQLvXY4DDW_OmBv77zX82YK3oVtyTja6xwnzLc2YbT_Trbx0v5IN9b0CmApq-D1vSCnn3ONZKdIrqbAbUgzWdkbo0GDFBV99R96leZc41dKS61BKbkTHt5RLieiHIaHZQTYXXl2V57dlVfZl39v_0D-aUv9BvgelWY</recordid><startdate>20221101</startdate><enddate>20221101</enddate><creator>Ye, Zhixin</creator><creator>Wang, Songzi</creator><creator>Huang, Xiaoqing</creator><creator>Chen, Peisong</creator><creator>Deng, Langhui</creator><creator>Li, Shiqi</creator><creator>Lin, Suiwen</creator><creator>Wang, Zilian</creator><creator>Liu, Bin</creator><general>American Diabetes Association</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0208-6066</orcidid></search><sort><creationdate>20221101</creationdate><title>Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus</title><author>Ye, Zhixin ; Wang, Songzi ; Huang, Xiaoqing ; Chen, Peisong ; Deng, Langhui ; Li, Shiqi ; Lin, Suiwen ; Wang, Zilian ; Liu, Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-4cd9a625a7ae6e4989d3525fd8c07de06a5d29372e064315c53f1ce67caff0903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>AMP-Activated Protein Kinases - metabolism</topic><topic>Chromosome 5</topic><topic>Diabetes mellitus</topic><topic>Diabetes, Gestational - metabolism</topic><topic>Exosomes</topic><topic>Exosomes - genetics</topic><topic>Exosomes - metabolism</topic><topic>Female</topic><topic>Gestational diabetes</topic><topic>Glucose - metabolism</topic><topic>Glucose tolerance</topic><topic>Humans</topic><topic>Insulin</topic><topic>Insulin - metabolism</topic><topic>Metabolism</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>Plasma</topic><topic>Pregnancy</topic><topic>Signal transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ye, Zhixin</creatorcontrib><creatorcontrib>Wang, Songzi</creatorcontrib><creatorcontrib>Huang, Xiaoqing</creatorcontrib><creatorcontrib>Chen, Peisong</creatorcontrib><creatorcontrib>Deng, Langhui</creatorcontrib><creatorcontrib>Li, Shiqi</creatorcontrib><creatorcontrib>Lin, Suiwen</creatorcontrib><creatorcontrib>Wang, Zilian</creatorcontrib><creatorcontrib>Liu, Bin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Diabetes (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ye, Zhixin</au><au>Wang, Songzi</au><au>Huang, Xiaoqing</au><au>Chen, Peisong</au><au>Deng, Langhui</au><au>Li, Shiqi</au><au>Lin, Suiwen</au><au>Wang, Zilian</au><au>Liu, Bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus</atitle><jtitle>Diabetes (New York, N.Y.)</jtitle><addtitle>Diabetes</addtitle><date>2022-11-01</date><risdate>2022</risdate><volume>71</volume><issue>11</issue><spage>2272</spage><epage>2283</epage><pages>2272-2283</pages><issn>0012-1797</issn><eissn>1939-327X</eissn><abstract>To date, the miRNA expression profile of plasma exosomes in women whose pregnancy is complicated by gestational diabetes mellitus (GDM) has not been fully clarified. In this study, differentially expressed miRNAs in plasma exosomes were identified by high-throughput small-RNA sequencing in 12 pregnant women with GDM and 12 with normal glucose tolerance (NGT) and validated in 102 pregnant women with GDM and 101 with NGT. A total of 22 exosomal miRNAs were found, five of which were verified by real-time qPCR. Exosomal miR-423-5p was upregulated, whereas miR-122-5p, miR-148a-3p, miR-192-5p, and miR-99a-5p were downregulated in women whose pregnancy was complicated by GDM. IGF1R and GYS1 as target genes of miR-423-5p, and G6PC3 and FDFT1 as target genes of miR-122-5p were associated with insulin and AMPK signaling pathways and may participate in the regulation of metabolism in GDM. The five exosomal miRNAs had an area under the curve of 0.82 (95%CI, 0.73, ∼0.91) in early prediction of GDM. Our study demonstrates that dysregulated exosomal miRNAs in plasma from pregnant women with GDM might influence the insulin and AMPK signaling pathways and could contribute to the early prediction of GDM.</abstract><cop>United States</cop><pub>American Diabetes Association</pub><pmid>35926094</pmid><doi>10.2337/db21-0909</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-0208-6066</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | AMP-Activated Protein Kinases - metabolism Chromosome 5 Diabetes mellitus Diabetes, Gestational - metabolism Exosomes Exosomes - genetics Exosomes - metabolism Female Gestational diabetes Glucose - metabolism Glucose tolerance Humans Insulin Insulin - metabolism Metabolism MicroRNAs - metabolism miRNA Plasma Pregnancy Signal transduction |
| title | Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus |
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