Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay
•Similarity of the clinical manifestations of monkeypox with chickenpox.•Multiplex real-time TaqMan PCR assay was developed.•Variola, monkeypox and varicella-zoster virus differentiation. A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), m...
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| Veröffentlicht in: | Journal of virological methods 2016-10, Vol.236, p.215-220 |
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| creator | Maksyutov, Rinat A. Gavrilova, Elena V. Shchelkunov, Sergei N. |
| description | •Similarity of the clinical manifestations of monkeypox with chickenpox.•Multiplex real-time TaqMan PCR assay was developed.•Variola, monkeypox and varicella-zoster virus differentiation.
A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), monkeypox virus (MPXV), and varicella-zoster virus (VZV) utilizing multiplex real-time TaqMan PCR assay was developed. Four hybridization probes with various fluorescent dyes and the corresponding fluorescence quenchers were simultaneously used for the assay. The hybridization probes specific for the VARV sequence contained FAM/BHQ1 as a dye/quencher pair; MPXV-specific, JOE/BHQ1; VZV-specific, TAMRA/BHQ2; and internal control-specific, Cy5/BHQ3. The specificity and sensitivity of the developed method were assessed by analyzing DNA of 32 strains belonging to orthopoxvirus and herpesvirus species. |
| doi_str_mv | 10.1016/j.jviromet.2016.07.024 |
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A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), monkeypox virus (MPXV), and varicella-zoster virus (VZV) utilizing multiplex real-time TaqMan PCR assay was developed. Four hybridization probes with various fluorescent dyes and the corresponding fluorescence quenchers were simultaneously used for the assay. The hybridization probes specific for the VARV sequence contained FAM/BHQ1 as a dye/quencher pair; MPXV-specific, JOE/BHQ1; VZV-specific, TAMRA/BHQ2; and internal control-specific, Cy5/BHQ3. The specificity and sensitivity of the developed method were assessed by analyzing DNA of 32 strains belonging to orthopoxvirus and herpesvirus species.</description><identifier>ISSN: 0166-0934</identifier><identifier>ISSN: 1879-0984</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/j.jviromet.2016.07.024</identifier><identifier>PMID: 27477914</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Herpesviridae ; Herpesvirus 3, Human - classification ; Herpesvirus 3, Human - genetics ; Herpesvirus 3, Human - isolation & purification ; Monkeypox virus ; Monkeypox virus - classification ; Monkeypox virus - genetics ; Monkeypox virus - isolation & purification ; Mpox (monkeypox) ; Multiplex Polymerase Chain Reaction - methods ; Orthopoxvirus ; Real-time PCR ; Real-Time Polymerase Chain Reaction - methods ; Sensitivity and Specificity ; Varicella-zoster virus ; Variola ; Variola virus ; Variola virus - classification ; Variola virus - genetics ; Variola virus - isolation & purification ; Virology - methods</subject><ispartof>Journal of virological methods, 2016-10, Vol.236, p.215-220</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><rights>2016 Elsevier B.V. All rights reserved. 2016 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c570t-18ce17c20fc5b0d200fad6e6e168b65ade2d576e16ebe01a3d2b34573d0914ec3</citedby><cites>FETCH-LOGICAL-c570t-18ce17c20fc5b0d200fad6e6e168b65ade2d576e16ebe01a3d2b34573d0914ec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0166093416300672$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27477914$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maksyutov, Rinat A.</creatorcontrib><creatorcontrib>Gavrilova, Elena V.</creatorcontrib><creatorcontrib>Shchelkunov, Sergei N.</creatorcontrib><title>Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>•Similarity of the clinical manifestations of monkeypox with chickenpox.•Multiplex real-time TaqMan PCR assay was developed.•Variola, monkeypox and varicella-zoster virus differentiation.
A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), monkeypox virus (MPXV), and varicella-zoster virus (VZV) utilizing multiplex real-time TaqMan PCR assay was developed. Four hybridization probes with various fluorescent dyes and the corresponding fluorescence quenchers were simultaneously used for the assay. The hybridization probes specific for the VARV sequence contained FAM/BHQ1 as a dye/quencher pair; MPXV-specific, JOE/BHQ1; VZV-specific, TAMRA/BHQ2; and internal control-specific, Cy5/BHQ3. The specificity and sensitivity of the developed method were assessed by analyzing DNA of 32 strains belonging to orthopoxvirus and herpesvirus species.</description><subject>Herpesviridae</subject><subject>Herpesvirus 3, Human - classification</subject><subject>Herpesvirus 3, Human - genetics</subject><subject>Herpesvirus 3, Human - isolation & purification</subject><subject>Monkeypox virus</subject><subject>Monkeypox virus - classification</subject><subject>Monkeypox virus - genetics</subject><subject>Monkeypox virus - isolation & purification</subject><subject>Mpox (monkeypox)</subject><subject>Multiplex Polymerase Chain Reaction - methods</subject><subject>Orthopoxvirus</subject><subject>Real-time PCR</subject><subject>Real-Time Polymerase Chain Reaction - methods</subject><subject>Sensitivity and Specificity</subject><subject>Varicella-zoster virus</subject><subject>Variola</subject><subject>Variola virus</subject><subject>Variola virus - classification</subject><subject>Variola virus - genetics</subject><subject>Variola virus - isolation & purification</subject><subject>Virology - methods</subject><issn>0166-0934</issn><issn>1879-0984</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAQtRAVXQp_ofKRQxPGTmInFwRaFVqpUis-zpZjT8BLEgc7WXX59Xi7bVVOPY1n5s2beX6EnDLIGTDxfpNvti74AeecpzwHmQMvX5AVq2WTQVOXL8kqNUR6F-UxeR3jBgAqWRSvyDGXpZQNK1dk-TahcRizuI-dM9S6rsOA4-z07PxIfUe3Ojjf6zM6-PE37iZ_e0b1aO_qBvteZ399nDHQdNISMdJ2R4eln93U4y0NqPtsdgPSm_VXqmPUuzfkqNN9xLf38YT8-Hz-fX2RXV1_uVx_uspMJWHOWG2QScOhM1ULlgN02goUyETdikpb5LaS-xRbBKYLy9uiTBotJHFoihPy4cA7Le2A1iRVQfdqCm7QYae8dur_zuh-qZ9-qxrBGyhFInh3TxD8nwXjrAYX7ySP6JeoWM1lA8DTzueh6WpRyqpMUHGAmuBjDNg9XsRA7e1VG_Vgr9rbq0CqZG8aPH2q53Hswc8E-HgAYPrVrcOgYnJ3NGhdQDMr691zO_4BSPu98Q</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Maksyutov, Rinat A.</creator><creator>Gavrilova, Elena V.</creator><creator>Shchelkunov, Sergei N.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>20161001</creationdate><title>Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay</title><author>Maksyutov, Rinat A. ; Gavrilova, Elena V. ; Shchelkunov, Sergei N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c570t-18ce17c20fc5b0d200fad6e6e168b65ade2d576e16ebe01a3d2b34573d0914ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Herpesviridae</topic><topic>Herpesvirus 3, Human - classification</topic><topic>Herpesvirus 3, Human - genetics</topic><topic>Herpesvirus 3, Human - isolation & purification</topic><topic>Monkeypox virus</topic><topic>Monkeypox virus - classification</topic><topic>Monkeypox virus - genetics</topic><topic>Monkeypox virus - isolation & purification</topic><topic>Mpox (monkeypox)</topic><topic>Multiplex Polymerase Chain Reaction - methods</topic><topic>Orthopoxvirus</topic><topic>Real-time PCR</topic><topic>Real-Time Polymerase Chain Reaction - methods</topic><topic>Sensitivity and Specificity</topic><topic>Varicella-zoster virus</topic><topic>Variola</topic><topic>Variola virus</topic><topic>Variola virus - classification</topic><topic>Variola virus - genetics</topic><topic>Variola virus - isolation & purification</topic><topic>Virology - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maksyutov, Rinat A.</creatorcontrib><creatorcontrib>Gavrilova, Elena V.</creatorcontrib><creatorcontrib>Shchelkunov, Sergei N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maksyutov, Rinat A.</au><au>Gavrilova, Elena V.</au><au>Shchelkunov, Sergei N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>236</volume><spage>215</spage><epage>220</epage><pages>215-220</pages><issn>0166-0934</issn><issn>1879-0984</issn><eissn>1879-0984</eissn><abstract>•Similarity of the clinical manifestations of monkeypox with chickenpox.•Multiplex real-time TaqMan PCR assay was developed.•Variola, monkeypox and varicella-zoster virus differentiation.
A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), monkeypox virus (MPXV), and varicella-zoster virus (VZV) utilizing multiplex real-time TaqMan PCR assay was developed. Four hybridization probes with various fluorescent dyes and the corresponding fluorescence quenchers were simultaneously used for the assay. The hybridization probes specific for the VARV sequence contained FAM/BHQ1 as a dye/quencher pair; MPXV-specific, JOE/BHQ1; VZV-specific, TAMRA/BHQ2; and internal control-specific, Cy5/BHQ3. The specificity and sensitivity of the developed method were assessed by analyzing DNA of 32 strains belonging to orthopoxvirus and herpesvirus species.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27477914</pmid><doi>10.1016/j.jviromet.2016.07.024</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Herpesviridae Herpesvirus 3, Human - classification Herpesvirus 3, Human - genetics Herpesvirus 3, Human - isolation & purification Monkeypox virus Monkeypox virus - classification Monkeypox virus - genetics Monkeypox virus - isolation & purification Mpox (monkeypox) Multiplex Polymerase Chain Reaction - methods Orthopoxvirus Real-time PCR Real-Time Polymerase Chain Reaction - methods Sensitivity and Specificity Varicella-zoster virus Variola Variola virus Variola virus - classification Variola virus - genetics Variola virus - isolation & purification Virology - methods |
| title | Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay |
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