Bioconjugation Strategies for Tobacco Mild Green Mosaic Virus

Tobacco mild green mosaic virus (TMGMV) is a plant virus closely related to Tobacco mosaic virus (TMV), sharing many of its structural and chemical features. These rod‐shaped viruses, comprised of 2130 identical coat protein subunits, have been utilized as nanotechnological platforms for a myriad of applications, ranging from drug delivery to precision agriculture. This versatility for functionalization is due to their chemically active external and internal surfaces. While both viruses are similar, they do exhibit some key differences in their surface chemistry, suggesting the reactive residue distribution on TMGMV should not overlap with TMV. In this work, we focused on the establishment and refinement of chemical bioconjugation strategies to load molecules into or onto TMGMV for targeted delivery. A combination of NHS, EDC, and diazo coupling reactions in combination with click chemistry were used to modify the N‐terminus, glutamic/aspartic acid residues, and tyrosines in TMGMV. We report loading with over 600 moieties per TMGMV via diazo‐coupling, which is a >3‐fold increase compared to previous studies. We also report that cargo can be loaded to the solvent‐exposed N‐terminus and carboxylates on the exterior/interior surfaces. Mass spectrometry revealed the most reactive sites to be Y12 and Y72, both tyrosine side chains are located on the exterior surface. For the carboxylates, interior E106 (66.53 %) was the most reactive for EDC‐propargylamine coupled reactions, with the exterior E145 accounting for >15 % reactivity, overturning previous assumptions that only interior glutamic acid residues are accessible. A deeper understanding of the chemical properties of TMGMV further enables its functionalization and use as a multifunctional nanocarrier platform for applications in medicine and precision farming. Using the plant virus nanoparticle TMGMV, we systematically assessed which amino acids are solvent exposed and addressable by bioconjugation strategies. We determined that glutamic acids on the exterior and interior surfaces, tyrosines on the exterior surface as well as the exterior and solvent‐exposed N‐terminus are reactive but to varying degrees (Tyr>Glu>N‐term). Bioconjugation reactions and proteomics paired with structural analysis of TMGMV provided rationale for the underlying reactivity of target sites. A deeper understanding of the chemical properties of TMGMV further enables its functionalization and use as a multifunctional nanocarrier platf