The Effects of Genistein at Different Concentrations on MCF-7 Breast Cancer Cells and BJ Dermal Fibroblasts

This study aimed to evaluate the safety and potential use of soy isoflavones in the treatment of skin problems, difficult-to-heal wounds and postoperative scars in women after the oncological treatment of breast cancer. The effects of different concentrations of genistein as a representative of soy...

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Veröffentlicht in:International journal of molecular sciences 2022-10, Vol.23 (20), p.12360
Hauptverfasser: Pawlicka, Magda Aleksandra, Zmorzyński, Szymon, Popek-Marciniec, Sylwia, Filip, Agata Anna
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container_issue 20
container_start_page 12360
container_title International journal of molecular sciences
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creator Pawlicka, Magda Aleksandra
Zmorzyński, Szymon
Popek-Marciniec, Sylwia
Filip, Agata Anna
description This study aimed to evaluate the safety and potential use of soy isoflavones in the treatment of skin problems, difficult-to-heal wounds and postoperative scars in women after the oncological treatment of breast cancer. The effects of different concentrations of genistein as a representative of soy isoflavonoids on MCF-7 tumor cells and BJ skin fibroblasts cultured in vitro were assessed. Genistein affects both healthy dermal BJ fibroblasts and cancerous MCF-7 cells. The effect of the tested isoflavonoid is closely related to its concentration. High concentrations of genistein destroy MCF-7 cancer cells, regardless of the exposure time, with a much greater effect on reducing cancer cell numbers at longer times (48 h). Lower concentrations of genistein (10 and 20 μM) increase the abundance of dermal fibroblasts. However, higher concentrations of genistein (50 μM and higher) are detrimental to fibroblasts at longer exposure times (48 h). Our studies indicate that although genistein shows high potential for use in the treatment of skin problems, wounds and surgical scars in women during and after breast cancer treatment, it is not completely safe. Introducing isoflavonoids to treatment requires further research into their mechanisms of action at the molecular level, taking into account genetic and immunological aspects. It is also necessary to conduct research in in vivo models, which will allow for eliminating adverse side effects of therapy.
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source MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects Apoptosis
Breast cancer
Cancer therapies
Cell cycle
Cell growth
Cytotoxicity
Estrogens
Fibroblasts
Gene expression
Genistein
Immunology
Isoflavones
Isoflavonoids
Proteins
Scars
Skin
Surgery
Tumor cells
Wound healing
Wounds
title The Effects of Genistein at Different Concentrations on MCF-7 Breast Cancer Cells and BJ Dermal Fibroblasts
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