Mechanical Stimulation Alters Chronic Ethanol-Induced Changes to VTA GABA Neurons, NAc DA Release and Measures of Withdrawal
Therapeutic activation of mechanoreceptors (MStim) in osteopathy, chiropractic and acupuncture has been in use for hundreds of years with a myriad of positive outcomes. It has been previously shown to modulate the firing rate of neurons in the ventral tegmental area (VTA) and dopamine (DA) release i...
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| Veröffentlicht in: | International journal of molecular sciences 2022-10, Vol.23 (20), p.12630 |
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| creator | Bills, Kyle B. Otteson, Dallin Z. Jones, Gavin C. Brundage, James N. Baldwin, Emily K. Small, Christina A. Kim, Hee Young Yorgason, Jordan T. Blotter, Jonathan D. Steffensen, Scott C. |
| description | Therapeutic activation of mechanoreceptors (MStim) in osteopathy, chiropractic and acupuncture has been in use for hundreds of years with a myriad of positive outcomes. It has been previously shown to modulate the firing rate of neurons in the ventral tegmental area (VTA) and dopamine (DA) release in the nucleus accumbens (NAc), an area of interest in alcohol-use disorder (AUD). In this study, we examined the effects of MStim on VTA GABA neuron firing rate, DA release in the NAc, and behavior during withdrawal from chronic EtOH exposure in a rat model. We demonstrate that concurrent administration of MStim and EtOH significantly reduced adaptations in VTA GABA neurons and DA release in response to a reinstatement dose of EtOH (2.5 g/kg). Behavioral indices of EtOH withdrawal (rearing, open-field crosses, tail stiffness, gait, and anxiety) were substantively ameliorated with concurrent application of MStim. Additionally, MStim significantly increased the overall frequency of ultrasonic vocalizations, suggesting an increased positive affective state. |
| doi_str_mv | 10.3390/ijms232012630 |
| format | Article |
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It has been previously shown to modulate the firing rate of neurons in the ventral tegmental area (VTA) and dopamine (DA) release in the nucleus accumbens (NAc), an area of interest in alcohol-use disorder (AUD). In this study, we examined the effects of MStim on VTA GABA neuron firing rate, DA release in the NAc, and behavior during withdrawal from chronic EtOH exposure in a rat model. We demonstrate that concurrent administration of MStim and EtOH significantly reduced adaptations in VTA GABA neurons and DA release in response to a reinstatement dose of EtOH (2.5 g/kg). Behavioral indices of EtOH withdrawal (rearing, open-field crosses, tail stiffness, gait, and anxiety) were substantively ameliorated with concurrent application of MStim. Additionally, MStim significantly increased the overall frequency of ultrasonic vocalizations, suggesting an increased positive affective state.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms232012630</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Adaptation ; Behavior ; Cocaine ; Desensitization (Psychology) ; Dopamine ; Drug dosages ; Drug withdrawal ; Emotional behavior ; Ethanol ; Firing rate ; Gait ; Mechanical stimuli ; Mechanoreceptors ; Narcotics ; Neurons ; Nucleus accumbens ; Reinstatement ; Stiffness ; Ventral tegmentum ; γ-Aminobutyric acid</subject><ispartof>International journal of molecular sciences, 2022-10, Vol.23 (20), p.12630</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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Otteson, Dallin Z. ; Jones, Gavin C. ; Brundage, James N. ; Baldwin, Emily K. ; Small, Christina A. ; Kim, Hee Young ; Yorgason, Jordan T. ; Blotter, Jonathan D. ; Steffensen, Scott C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-6ca053e2aed46fff60a4df23614a5ae23974629323c77eb29fe09fd499460d643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adaptation</topic><topic>Behavior</topic><topic>Cocaine</topic><topic>Desensitization (Psychology)</topic><topic>Dopamine</topic><topic>Drug dosages</topic><topic>Drug withdrawal</topic><topic>Emotional behavior</topic><topic>Ethanol</topic><topic>Firing rate</topic><topic>Gait</topic><topic>Mechanical stimuli</topic><topic>Mechanoreceptors</topic><topic>Narcotics</topic><topic>Neurons</topic><topic>Nucleus accumbens</topic><topic>Reinstatement</topic><topic>Stiffness</topic><topic>Ventral tegmentum</topic><topic>γ-Aminobutyric acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bills, Kyle B.</creatorcontrib><creatorcontrib>Otteson, Dallin Z.</creatorcontrib><creatorcontrib>Jones, Gavin C.</creatorcontrib><creatorcontrib>Brundage, James N.</creatorcontrib><creatorcontrib>Baldwin, Emily K.</creatorcontrib><creatorcontrib>Small, Christina A.</creatorcontrib><creatorcontrib>Kim, Hee Young</creatorcontrib><creatorcontrib>Yorgason, Jordan T.</creatorcontrib><creatorcontrib>Blotter, Jonathan D.</creatorcontrib><creatorcontrib>Steffensen, Scott C.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bills, Kyle B.</au><au>Otteson, Dallin Z.</au><au>Jones, Gavin C.</au><au>Brundage, James N.</au><au>Baldwin, Emily K.</au><au>Small, Christina A.</au><au>Kim, Hee Young</au><au>Yorgason, Jordan T.</au><au>Blotter, Jonathan D.</au><au>Steffensen, Scott C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanical Stimulation Alters Chronic Ethanol-Induced Changes to VTA GABA Neurons, NAc DA Release and Measures of Withdrawal</atitle><jtitle>International journal of molecular sciences</jtitle><date>2022-10-20</date><risdate>2022</risdate><volume>23</volume><issue>20</issue><spage>12630</spage><pages>12630-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Therapeutic activation of mechanoreceptors (MStim) in osteopathy, chiropractic and acupuncture has been in use for hundreds of years with a myriad of positive outcomes. 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| subjects | Adaptation Behavior Cocaine Desensitization (Psychology) Dopamine Drug dosages Drug withdrawal Emotional behavior Ethanol Firing rate Gait Mechanical stimuli Mechanoreceptors Narcotics Neurons Nucleus accumbens Reinstatement Stiffness Ventral tegmentum γ-Aminobutyric acid |
| title | Mechanical Stimulation Alters Chronic Ethanol-Induced Changes to VTA GABA Neurons, NAc DA Release and Measures of Withdrawal |
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