Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans
Kae1 is a subunit of the highly evolutionarily conserved KEOPS/EKC complex, which is involved in universal (t6A37) tRNA modification. Several reports have discussed the participation of this complex in transcription regulation in yeast and human cells, including our previous observations of KaeA, an...
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| Veröffentlicht in: | International journal of molecular sciences 2022-09, Vol.23 (19), p.11138 |
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| description | Kae1 is a subunit of the highly evolutionarily conserved KEOPS/EKC complex, which is involved in universal (t6A37) tRNA modification. Several reports have discussed the participation of this complex in transcription regulation in yeast and human cells, including our previous observations of KaeA, an Aspergillus nidulans homologue of Kae1p. The aim of this project was to confirm the role of KaeA in transcription, employing high-throughput transcriptomic (RNA-Seq and ChIP-Seq) and proteomic (LC-MS) analysis. We confirmed that KaeA is a subunit of the KEOPS complex in A. nidulans. An analysis of kaeA19 and kaeA25 mutants showed that, although the (t6A37) tRNA modification is unaffected in both mutants, they reveal significantly altered transcriptomes compared to the wild type. The finding that KaeA is localized in chromatin and identifying its protein partners allows us to postulate an additional nuclear function for the protein. Our data shed light on the universal bi-functional role of this factor and proves that the activity of this protein is not limited to tRNA modification in cytoplasm, but also affects the transcriptional activity of a number of nuclear genes. Data are available via the NCBI’s GEO database under identifiers GSE206830 (RNA-Seq) and GSE206874 (ChIP-Seq), and via ProteomeXchange with identifier PXD034554 (proteomic). |
| doi_str_mv | 10.3390/ijms231911138 |
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Several reports have discussed the participation of this complex in transcription regulation in yeast and human cells, including our previous observations of KaeA, an Aspergillus nidulans homologue of Kae1p. The aim of this project was to confirm the role of KaeA in transcription, employing high-throughput transcriptomic (RNA-Seq and ChIP-Seq) and proteomic (LC-MS) analysis. We confirmed that KaeA is a subunit of the KEOPS complex in A. nidulans. An analysis of kaeA19 and kaeA25 mutants showed that, although the (t6A37) tRNA modification is unaffected in both mutants, they reveal significantly altered transcriptomes compared to the wild type. The finding that KaeA is localized in chromatin and identifying its protein partners allows us to postulate an additional nuclear function for the protein. Our data shed light on the universal bi-functional role of this factor and proves that the activity of this protein is not limited to tRNA modification in cytoplasm, but also affects the transcriptional activity of a number of nuclear genes. Data are available via the NCBI’s GEO database under identifiers GSE206830 (RNA-Seq) and GSE206874 (ChIP-Seq), and via ProteomeXchange with identifier PXD034554 (proteomic).</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms231911138</identifier><identifier>PMID: 36232439</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Adenosine ; Amino acids ; Aspergillus nidulans ; Chromatin ; Cytoplasm ; Experiments ; Gene expression ; Gene regulation ; Genomes ; Hypotheses ; Kinases ; Metabolism ; Mutants ; Mutation ; Proteins ; Proteomics ; Transcription ; Transcriptomes ; Transcriptomics ; Transfer RNA ; tRNA ; tRNA Ala ; Yeast</subject><ispartof>International journal of molecular sciences, 2022-09, Vol.23 (19), p.11138</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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Our data shed light on the universal bi-functional role of this factor and proves that the activity of this protein is not limited to tRNA modification in cytoplasm, but also affects the transcriptional activity of a number of nuclear genes. 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Koper, Michal ; Bogdanowicz, Albert ; Weglenski, Piotr ; Dzikowska, Agnieszka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c278t-179e03275936e3c4d9690d742aeac79335da0544cbb52e084f37e48eb7ffa9933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adenosine</topic><topic>Amino acids</topic><topic>Aspergillus nidulans</topic><topic>Chromatin</topic><topic>Cytoplasm</topic><topic>Experiments</topic><topic>Gene expression</topic><topic>Gene regulation</topic><topic>Genomes</topic><topic>Hypotheses</topic><topic>Kinases</topic><topic>Metabolism</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Transcription</topic><topic>Transcriptomes</topic><topic>Transcriptomics</topic><topic>Transfer RNA</topic><topic>tRNA</topic><topic>tRNA Ala</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gawlik, Joanna</creatorcontrib><creatorcontrib>Koper, Michal</creatorcontrib><creatorcontrib>Bogdanowicz, Albert</creatorcontrib><creatorcontrib>Weglenski, Piotr</creatorcontrib><creatorcontrib>Dzikowska, Agnieszka</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gawlik, Joanna</au><au>Koper, Michal</au><au>Bogdanowicz, Albert</au><au>Weglenski, Piotr</au><au>Dzikowska, Agnieszka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans</atitle><jtitle>International journal of molecular sciences</jtitle><date>2022-09-22</date><risdate>2022</risdate><volume>23</volume><issue>19</issue><spage>11138</spage><pages>11138-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Kae1 is a subunit of the highly evolutionarily conserved KEOPS/EKC complex, which is involved in universal (t6A37) tRNA modification. 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Our data shed light on the universal bi-functional role of this factor and proves that the activity of this protein is not limited to tRNA modification in cytoplasm, but also affects the transcriptional activity of a number of nuclear genes. Data are available via the NCBI’s GEO database under identifiers GSE206830 (RNA-Seq) and GSE206874 (ChIP-Seq), and via ProteomeXchange with identifier PXD034554 (proteomic).</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>36232439</pmid><doi>10.3390/ijms231911138</doi><orcidid>https://orcid.org/0000-0003-3228-6253</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine Amino acids Aspergillus nidulans Chromatin Cytoplasm Experiments Gene expression Gene regulation Genomes Hypotheses Kinases Metabolism Mutants Mutation Proteins Proteomics Transcription Transcriptomes Transcriptomics Transfer RNA tRNA tRNA Ala Yeast |
| title | Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans |
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