Staphylococcus aureus Keratitis in Taiwan: Genotyping, Antibiotic Susceptibility, and Clinical Features
Staphylococcus aureus is an important pathogen for keratitis, a vision-threatening disease. We aimed to investigate the genotyping, antibiotic susceptibility, and clinical features of S. aureus keratitis, and to explore the possible role of Panton–Valentine leucocidin (PVL), a major virulence factor...
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| Veröffentlicht in: | International journal of molecular sciences 2022-10, Vol.23 (19), p.11703 |
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| creator | Hsiao, Ching-Hsi Kang, Eugene Yu-Chuan Yeh, Lung-Kun Ma, David H. K. Chen, Hung-Chi Hung, Kuo-Hsuan Huang, Yhu-Chering |
| description | Staphylococcus aureus is an important pathogen for keratitis, a vision-threatening disease. We aimed to investigate the genotyping, antibiotic susceptibility, and clinical features of S. aureus keratitis, and to explore the possible role of Panton–Valentine leucocidin (PVL), a major virulence factor of S. aureus. We recruited 49 patients with culture-proven S. aureus keratitis between 2013 and 2017 at Chang Gung Memorial Hospital, Taiwan. PVL gene, multilocus sequence type (MLST), staphylococcal cassette chromosome mec (SCCmec), and pulsed-field gel electrophoresis (PFGE) were performed. Antibiotic susceptibility was verified using disk diffusion/E test. There were 49 patients with S. aureus keratitis; 17 (34.7%) were caused by methicillin-resistant S. aureus (MRSA) and 9 (18.4%) isolates had PVL genes. The predominant genotyping of MRSA isolates was CC59/PFGE type D/SCCmec VT/PVL (+). All methicillin-sensitive S. aureus (MSSA) and approximately 60% MRSA were susceptible to fluoroquinolones. No significant differences in clinical features, treatments, and visual outcomes were observed between MRSA/MSSA or PVL(+)/PVL(−) groups. In Taiwan, approximately one third of S. aureus keratitis was caused by MRSA, mainly community-associated MRSA. Although MRSA isolates were more resistant than MSSA, clinical characteristics were similar between two groups. Fluoroquinolones could be good empiric antibiotics for S. aureus keratitis. |
| doi_str_mv | 10.3390/ijms231911703 |
| format | Article |
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K. ; Chen, Hung-Chi ; Hung, Kuo-Hsuan ; Huang, Yhu-Chering</creator><creatorcontrib>Hsiao, Ching-Hsi ; Kang, Eugene Yu-Chuan ; Yeh, Lung-Kun ; Ma, David H. K. ; Chen, Hung-Chi ; Hung, Kuo-Hsuan ; Huang, Yhu-Chering</creatorcontrib><description>Staphylococcus aureus is an important pathogen for keratitis, a vision-threatening disease. We aimed to investigate the genotyping, antibiotic susceptibility, and clinical features of S. aureus keratitis, and to explore the possible role of Panton–Valentine leucocidin (PVL), a major virulence factor of S. aureus. We recruited 49 patients with culture-proven S. aureus keratitis between 2013 and 2017 at Chang Gung Memorial Hospital, Taiwan. PVL gene, multilocus sequence type (MLST), staphylococcal cassette chromosome mec (SCCmec), and pulsed-field gel electrophoresis (PFGE) were performed. Antibiotic susceptibility was verified using disk diffusion/E test. There were 49 patients with S. aureus keratitis; 17 (34.7%) were caused by methicillin-resistant S. aureus (MRSA) and 9 (18.4%) isolates had PVL genes. The predominant genotyping of MRSA isolates was CC59/PFGE type D/SCCmec VT/PVL (+). All methicillin-sensitive S. aureus (MSSA) and approximately 60% MRSA were susceptible to fluoroquinolones. No significant differences in clinical features, treatments, and visual outcomes were observed between MRSA/MSSA or PVL(+)/PVL(−) groups. In Taiwan, approximately one third of S. aureus keratitis was caused by MRSA, mainly community-associated MRSA. Although MRSA isolates were more resistant than MSSA, clinical characteristics were similar between two groups. Fluoroquinolones could be good empiric antibiotics for S. aureus keratitis.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms231911703</identifier><identifier>PMID: 36233005</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Antibiotics ; Chromosomes ; Cloning ; Drug resistance ; Electrophoresis ; Epidemiology ; Fluoroquinolones ; Gel electrophoresis ; Genes ; Genotyping ; Keratitis ; Methicillin ; Pathogens ; Patients ; Pulsed-field gel electrophoresis ; Risk factors ; Staphylococcus aureus ; Staphylococcus infections ; Ulcers ; Virulence ; Virulence factors ; Visual observation</subject><ispartof>International journal of molecular sciences, 2022-10, Vol.23 (19), p.11703</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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K.</creatorcontrib><creatorcontrib>Chen, Hung-Chi</creatorcontrib><creatorcontrib>Hung, Kuo-Hsuan</creatorcontrib><creatorcontrib>Huang, Yhu-Chering</creatorcontrib><title>Staphylococcus aureus Keratitis in Taiwan: Genotyping, Antibiotic Susceptibility, and Clinical Features</title><title>International journal of molecular sciences</title><description>Staphylococcus aureus is an important pathogen for keratitis, a vision-threatening disease. We aimed to investigate the genotyping, antibiotic susceptibility, and clinical features of S. aureus keratitis, and to explore the possible role of Panton–Valentine leucocidin (PVL), a major virulence factor of S. aureus. We recruited 49 patients with culture-proven S. aureus keratitis between 2013 and 2017 at Chang Gung Memorial Hospital, Taiwan. PVL gene, multilocus sequence type (MLST), staphylococcal cassette chromosome mec (SCCmec), and pulsed-field gel electrophoresis (PFGE) were performed. Antibiotic susceptibility was verified using disk diffusion/E test. There were 49 patients with S. aureus keratitis; 17 (34.7%) were caused by methicillin-resistant S. aureus (MRSA) and 9 (18.4%) isolates had PVL genes. The predominant genotyping of MRSA isolates was CC59/PFGE type D/SCCmec VT/PVL (+). All methicillin-sensitive S. aureus (MSSA) and approximately 60% MRSA were susceptible to fluoroquinolones. No significant differences in clinical features, treatments, and visual outcomes were observed between MRSA/MSSA or PVL(+)/PVL(−) groups. In Taiwan, approximately one third of S. aureus keratitis was caused by MRSA, mainly community-associated MRSA. Although MRSA isolates were more resistant than MSSA, clinical characteristics were similar between two groups. Fluoroquinolones could be good empiric antibiotics for S. aureus keratitis.</description><subject>Antibiotics</subject><subject>Chromosomes</subject><subject>Cloning</subject><subject>Drug resistance</subject><subject>Electrophoresis</subject><subject>Epidemiology</subject><subject>Fluoroquinolones</subject><subject>Gel electrophoresis</subject><subject>Genes</subject><subject>Genotyping</subject><subject>Keratitis</subject><subject>Methicillin</subject><subject>Pathogens</subject><subject>Patients</subject><subject>Pulsed-field gel electrophoresis</subject><subject>Risk factors</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><subject>Ulcers</subject><subject>Virulence</subject><subject>Virulence factors</subject><subject>Visual observation</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkUtLAzEUhYMo1tfSfcCNC0dvknnFhVCKLxRcWNchk0nblGkyJhml_96Uiqircy_343APB6FTApeMcbgyy1WgjHBCKmA76IDklGYAZbX7ax6hwxCWAJTRgu-jESspYwDFAZq_Rtkv1p1TTqkhYDl4neRJexlNNAEbi6fSfEp7je-1dXHdGzu_wGMbTWNcNAq_DkHpfrN2Jq4vsLQtnnTGGiU7fKdlTJbhGO3NZBf0ybceobe72-nkIXt-uX-cjJ8zxTiNWanILG9KzSrCFamLpqR5yaWqodFKk5kqOTBNW6gbovJat8ArClVb8AraRgE7Qjdb335oVrpV2kYvO9F7s5J-LZw04u_FmoWYuw_BiwpoRZPB-beBd--DDlGsTMrXddJqNwSRmILwRLOEnv1Dl27wNsXbUDmt8wKKRGVbSnkXgtezn2cIiE2F4k-F7AsVhI9j</recordid><startdate>20221003</startdate><enddate>20221003</enddate><creator>Hsiao, Ching-Hsi</creator><creator>Kang, Eugene Yu-Chuan</creator><creator>Yeh, Lung-Kun</creator><creator>Ma, David H. 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PVL gene, multilocus sequence type (MLST), staphylococcal cassette chromosome mec (SCCmec), and pulsed-field gel electrophoresis (PFGE) were performed. Antibiotic susceptibility was verified using disk diffusion/E test. There were 49 patients with S. aureus keratitis; 17 (34.7%) were caused by methicillin-resistant S. aureus (MRSA) and 9 (18.4%) isolates had PVL genes. The predominant genotyping of MRSA isolates was CC59/PFGE type D/SCCmec VT/PVL (+). All methicillin-sensitive S. aureus (MSSA) and approximately 60% MRSA were susceptible to fluoroquinolones. No significant differences in clinical features, treatments, and visual outcomes were observed between MRSA/MSSA or PVL(+)/PVL(−) groups. In Taiwan, approximately one third of S. aureus keratitis was caused by MRSA, mainly community-associated MRSA. Although MRSA isolates were more resistant than MSSA, clinical characteristics were similar between two groups. 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| subjects | Antibiotics Chromosomes Cloning Drug resistance Electrophoresis Epidemiology Fluoroquinolones Gel electrophoresis Genes Genotyping Keratitis Methicillin Pathogens Patients Pulsed-field gel electrophoresis Risk factors Staphylococcus aureus Staphylococcus infections Ulcers Virulence Virulence factors Visual observation |
| title | Staphylococcus aureus Keratitis in Taiwan: Genotyping, Antibiotic Susceptibility, and Clinical Features |
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