mRNA Capture Sequencing and RT-qPCR for the Detection of Pathognomonic, Novel, and Secondary Fusion Transcripts in FFPE Tissue: A Sarcoma Showcase

We assess the performance of mRNA capture sequencing to identify fusion transcripts in FFPE tissue of different sarcoma types, followed by RT-qPCR confirmation. To validate our workflow, six positive control tumors with a specific chromosomal rearrangement were analyzed using the TruSight RNA Pan-Ca...

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Veröffentlicht in:	International journal of molecular sciences 2022-10, Vol.23 (19), p.11007
Hauptverfasser:	Decock, Anneleen, Creytens, David, Lefever, Steve, Van der Meulen, Joni, Anckaert, Jasper, De Ganck, Ariane, Deleu, Jill, De Wilde, Bram, Fierro, Carolina, Kuersten, Scott, Luypaert, Manuel, Rottiers, Isabelle, Schroth, Gary P., Steyaert, Sandra, Vanderheyden, Katrien, Vanden Eynde, Eveline, Verniers, Kimberly, Verreth, Joke, Van Dorpe, Jo, Vandesompele, Jo
Format:	Artikel
Sprache:	eng
Schlagworte:	Alveoli Breakpoints Cancer Chromosome rearrangements Ewings sarcoma FLI-1 protein Fluorescence in situ hybridization FOXO1 protein Fusion protein Genes Genomes Hybridization Identification Morphology Patients Pax3 protein Rhabdomyosarcoma Transcriptomes Tumorigenesis Tumors Workflow
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