Comparative Metagenomics and Metabolomes Reveals Abnormal Metabolism Activity Is Associated with Gut Microbiota in Alzheimer’s Disease Mice
A common symptom in Alzheimer’s disease (AD) is cognitive decline, of which the potential pathogenesis remains unclear. In order to understand the mechanism of gut microbiota in AD, it is necessary to clarify the relationship between gut microbiota and metabolites. Behavioral tests, pathological exa...
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Veröffentlicht in: | International journal of molecular sciences 2022-10, Vol.23 (19), p.11560 |
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creator | Sun, Peilin Zhu, Hua Li, Xue Shi, Weixiong Guo, Yaxi Du, Xiaopeng Zhang, Ling Su, Lei Qin, Chuan |
description | A common symptom in Alzheimer’s disease (AD) is cognitive decline, of which the potential pathogenesis remains unclear. In order to understand the mechanism of gut microbiota in AD, it is necessary to clarify the relationship between gut microbiota and metabolites. Behavioral tests, pathological examination, metagenomics, and metabolomics were applied to analyze the difference of gut microbiota and metabolome between APPswe/PS1ΔE9 (PAP) mice with cognitive decline and age-matched controls, and their possible correlations. Our results showed that PAP mice and health mice had different structures of the bacterial communities in the gut. The abundances and diversities of the bacterial communities in health mice were higher than in PAP mice by metagenomics analysis. The abundances of Libanicoccus massiliensis, Paraprevotella clara, and Lactobacillus amylovorus were significantly increased in PAP mice, while the abundances of Turicibacter sanguinis, Dubosiella newyorkensis, and Prevotella oris were greatly reduced. Furthermore, PAP mice possessed peculiar metabolic phenotypes in stool, serum, and hippocampus relative to WT mice, as is demonstrated by alterations in neurotransmitters metabolism, lipid metabolism, aromatic amino acids metabolism, energy metabolism, vitamin digestion and absorption, and bile metabolism. Microbiota–host metabolic correlation analysis suggests that abnormal metabolism in stool, serum, and hippocampus of PAP mice may be modulated by the gut microbiota, especially T. sanguinis, D. newyorkensis, and P. oris. Therefore, abnormal metabolism activity is associated with gut microbiota in Alzheimer’s disease mice. Our results imply that modifying host metabolism through targeting gut microbiota may be a novel and viable strategy for the prevention and treatment of AD in the future. |
doi_str_mv | 10.3390/ijms231911560 |
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In order to understand the mechanism of gut microbiota in AD, it is necessary to clarify the relationship between gut microbiota and metabolites. Behavioral tests, pathological examination, metagenomics, and metabolomics were applied to analyze the difference of gut microbiota and metabolome between APPswe/PS1ΔE9 (PAP) mice with cognitive decline and age-matched controls, and their possible correlations. Our results showed that PAP mice and health mice had different structures of the bacterial communities in the gut. The abundances and diversities of the bacterial communities in health mice were higher than in PAP mice by metagenomics analysis. The abundances of Libanicoccus massiliensis, Paraprevotella clara, and Lactobacillus amylovorus were significantly increased in PAP mice, while the abundances of Turicibacter sanguinis, Dubosiella newyorkensis, and Prevotella oris were greatly reduced. Furthermore, PAP mice possessed peculiar metabolic phenotypes in stool, serum, and hippocampus relative to WT mice, as is demonstrated by alterations in neurotransmitters metabolism, lipid metabolism, aromatic amino acids metabolism, energy metabolism, vitamin digestion and absorption, and bile metabolism. Microbiota–host metabolic correlation analysis suggests that abnormal metabolism in stool, serum, and hippocampus of PAP mice may be modulated by the gut microbiota, especially T. sanguinis, D. newyorkensis, and P. oris. Therefore, abnormal metabolism activity is associated with gut microbiota in Alzheimer’s disease mice. Our results imply that modifying host metabolism through targeting gut microbiota may be a novel and viable strategy for the prevention and treatment of AD in the future.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms231911560</identifier><identifier>PMID: 36232865</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Alzheimer's disease ; Amino acids ; Animal cognition ; Correlation analysis ; Digestive system ; Energy metabolism ; Genes ; Hippocampus ; Hypotheses ; Information processing ; Intestinal microflora ; Lipid metabolism ; Lipids ; Memory ; Metabolism ; Metabolites ; Metabolomics ; Metagenomics ; Microbiota ; Neurotransmitters ; Pathogenesis ; Phenotypes ; Rodents ; Small intestine ; Taxonomy</subject><ispartof>International journal of molecular sciences, 2022-10, Vol.23 (19), p.11560</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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In order to understand the mechanism of gut microbiota in AD, it is necessary to clarify the relationship between gut microbiota and metabolites. Behavioral tests, pathological examination, metagenomics, and metabolomics were applied to analyze the difference of gut microbiota and metabolome between APPswe/PS1ΔE9 (PAP) mice with cognitive decline and age-matched controls, and their possible correlations. Our results showed that PAP mice and health mice had different structures of the bacterial communities in the gut. The abundances and diversities of the bacterial communities in health mice were higher than in PAP mice by metagenomics analysis. The abundances of Libanicoccus massiliensis, Paraprevotella clara, and Lactobacillus amylovorus were significantly increased in PAP mice, while the abundances of Turicibacter sanguinis, Dubosiella newyorkensis, and Prevotella oris were greatly reduced. Furthermore, PAP mice possessed peculiar metabolic phenotypes in stool, serum, and hippocampus relative to WT mice, as is demonstrated by alterations in neurotransmitters metabolism, lipid metabolism, aromatic amino acids metabolism, energy metabolism, vitamin digestion and absorption, and bile metabolism. Microbiota–host metabolic correlation analysis suggests that abnormal metabolism in stool, serum, and hippocampus of PAP mice may be modulated by the gut microbiota, especially T. sanguinis, D. newyorkensis, and P. oris. Therefore, abnormal metabolism activity is associated with gut microbiota in Alzheimer’s disease mice. Our results imply that modifying host metabolism through targeting gut microbiota may be a novel and viable strategy for the prevention and treatment of AD in the future.</description><subject>Alzheimer's disease</subject><subject>Amino acids</subject><subject>Animal cognition</subject><subject>Correlation analysis</subject><subject>Digestive system</subject><subject>Energy metabolism</subject><subject>Genes</subject><subject>Hippocampus</subject><subject>Hypotheses</subject><subject>Information processing</subject><subject>Intestinal microflora</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Memory</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Metagenomics</subject><subject>Microbiota</subject><subject>Neurotransmitters</subject><subject>Pathogenesis</subject><subject>Phenotypes</subject><subject>Rodents</subject><subject>Small intestine</subject><subject>Taxonomy</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkU1uFDEQhS0EIiGwZG-JDZsG_7Td7Q3SaAIhUiIkBGvLbVdnPGq3B9s9KKy4AAfgepwETxIQYeWS3-dX5VcIPafkFeeKvPbbkBmnilIhyQN0TFvGGkJk9_Cf-gg9yXlLCONMqMfoiMta9VIcox_rGHYmmeL3gC-hmCuYY_A2YzO7m4shTjFAxh9hD2bKeDXMMQUz_RF9Dnhl63tfrvF51XOO1psCDn_1ZYPPloIvvU1x8LEY7Ge8mr5twAdIv77_zPjUZzAZDgw8RY_G2gOe3Z0n6PO7t5_W75uLD2fn69VFY7lipeGib3s-tq6FkTjpbCec462wcpSWCkooVU65EdjQgevBGmVkb6zoiGI1Jn6C3tz67pYhgLMwl2QmvUs-mHSto_H6vjL7jb6Ke62EVIL21eDlnUGKXxbIRQefLUyTmSEuWbOOCaqUvEFf_Idu45Lm-r0D1bJeMCoq1dxSNaicE4x_h6FEHxat7y2a_wZG7Z5U</recordid><startdate>20221001</startdate><enddate>20221001</enddate><creator>Sun, Peilin</creator><creator>Zhu, Hua</creator><creator>Li, Xue</creator><creator>Shi, Weixiong</creator><creator>Guo, Yaxi</creator><creator>Du, Xiaopeng</creator><creator>Zhang, Ling</creator><creator>Su, Lei</creator><creator>Qin, Chuan</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20221001</creationdate><title>Comparative Metagenomics and Metabolomes Reveals Abnormal Metabolism Activity Is Associated with Gut Microbiota in Alzheimer’s Disease Mice</title><author>Sun, Peilin ; 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In order to understand the mechanism of gut microbiota in AD, it is necessary to clarify the relationship between gut microbiota and metabolites. Behavioral tests, pathological examination, metagenomics, and metabolomics were applied to analyze the difference of gut microbiota and metabolome between APPswe/PS1ΔE9 (PAP) mice with cognitive decline and age-matched controls, and their possible correlations. Our results showed that PAP mice and health mice had different structures of the bacterial communities in the gut. The abundances and diversities of the bacterial communities in health mice were higher than in PAP mice by metagenomics analysis. The abundances of Libanicoccus massiliensis, Paraprevotella clara, and Lactobacillus amylovorus were significantly increased in PAP mice, while the abundances of Turicibacter sanguinis, Dubosiella newyorkensis, and Prevotella oris were greatly reduced. Furthermore, PAP mice possessed peculiar metabolic phenotypes in stool, serum, and hippocampus relative to WT mice, as is demonstrated by alterations in neurotransmitters metabolism, lipid metabolism, aromatic amino acids metabolism, energy metabolism, vitamin digestion and absorption, and bile metabolism. Microbiota–host metabolic correlation analysis suggests that abnormal metabolism in stool, serum, and hippocampus of PAP mice may be modulated by the gut microbiota, especially T. sanguinis, D. newyorkensis, and P. oris. Therefore, abnormal metabolism activity is associated with gut microbiota in Alzheimer’s disease mice. Our results imply that modifying host metabolism through targeting gut microbiota may be a novel and viable strategy for the prevention and treatment of AD in the future.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>36232865</pmid><doi>10.3390/ijms231911560</doi><oa>free_for_read</oa></addata></record> |
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subjects | Alzheimer's disease Amino acids Animal cognition Correlation analysis Digestive system Energy metabolism Genes Hippocampus Hypotheses Information processing Intestinal microflora Lipid metabolism Lipids Memory Metabolism Metabolites Metabolomics Metagenomics Microbiota Neurotransmitters Pathogenesis Phenotypes Rodents Small intestine Taxonomy |
title | Comparative Metagenomics and Metabolomes Reveals Abnormal Metabolism Activity Is Associated with Gut Microbiota in Alzheimer’s Disease Mice |
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