Comparative Metagenomics and Metabolomes Reveals Abnormal Metabolism Activity Is Associated with Gut Microbiota in Alzheimer’s Disease Mice

A common symptom in Alzheimer’s disease (AD) is cognitive decline, of which the potential pathogenesis remains unclear. In order to understand the mechanism of gut microbiota in AD, it is necessary to clarify the relationship between gut microbiota and metabolites. Behavioral tests, pathological exa...

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Veröffentlicht in:International journal of molecular sciences 2022-10, Vol.23 (19), p.11560
Hauptverfasser: Sun, Peilin, Zhu, Hua, Li, Xue, Shi, Weixiong, Guo, Yaxi, Du, Xiaopeng, Zhang, Ling, Su, Lei, Qin, Chuan
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container_issue 19
container_start_page 11560
container_title International journal of molecular sciences
container_volume 23
creator Sun, Peilin
Zhu, Hua
Li, Xue
Shi, Weixiong
Guo, Yaxi
Du, Xiaopeng
Zhang, Ling
Su, Lei
Qin, Chuan
description A common symptom in Alzheimer’s disease (AD) is cognitive decline, of which the potential pathogenesis remains unclear. In order to understand the mechanism of gut microbiota in AD, it is necessary to clarify the relationship between gut microbiota and metabolites. Behavioral tests, pathological examination, metagenomics, and metabolomics were applied to analyze the difference of gut microbiota and metabolome between APPswe/PS1ΔE9 (PAP) mice with cognitive decline and age-matched controls, and their possible correlations. Our results showed that PAP mice and health mice had different structures of the bacterial communities in the gut. The abundances and diversities of the bacterial communities in health mice were higher than in PAP mice by metagenomics analysis. The abundances of Libanicoccus massiliensis, Paraprevotella clara, and Lactobacillus amylovorus were significantly increased in PAP mice, while the abundances of Turicibacter sanguinis, Dubosiella newyorkensis, and Prevotella oris were greatly reduced. Furthermore, PAP mice possessed peculiar metabolic phenotypes in stool, serum, and hippocampus relative to WT mice, as is demonstrated by alterations in neurotransmitters metabolism, lipid metabolism, aromatic amino acids metabolism, energy metabolism, vitamin digestion and absorption, and bile metabolism. Microbiota–host metabolic correlation analysis suggests that abnormal metabolism in stool, serum, and hippocampus of PAP mice may be modulated by the gut microbiota, especially T. sanguinis, D. newyorkensis, and P. oris. Therefore, abnormal metabolism activity is associated with gut microbiota in Alzheimer’s disease mice. Our results imply that modifying host metabolism through targeting gut microbiota may be a novel and viable strategy for the prevention and treatment of AD in the future.
doi_str_mv 10.3390/ijms231911560
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subjects Alzheimer's disease
Amino acids
Animal cognition
Correlation analysis
Digestive system
Energy metabolism
Genes
Hippocampus
Hypotheses
Information processing
Intestinal microflora
Lipid metabolism
Lipids
Memory
Metabolism
Metabolites
Metabolomics
Metagenomics
Microbiota
Neurotransmitters
Pathogenesis
Phenotypes
Rodents
Small intestine
Taxonomy
title Comparative Metagenomics and Metabolomes Reveals Abnormal Metabolism Activity Is Associated with Gut Microbiota in Alzheimer’s Disease Mice
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