In vitro antibacterial effect of probiotics against Carbapenamase-producing multidrug-resistant Klebsiella pneumoniae clinical isolates, Cairo, Egypt
Background Searching for a non-antibiotic therapeutic option such as probiotics is gaining momentum nowadays. We aimed to evaluate the in vitro antibacterial ability of cell-free supernatant (CFS) of selected Lactobacillus strains (with probiotic properties) against clinical isolates of OXA-48-produ...
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| Veröffentlicht in: | Journal of the Egyptian Public Health Association 2022-10, Vol.97 (1), p.19-19, Article 19 |
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| creator | Abdelhalim, Mona Mohiedden Saafan, Ghada Samy El-Sayed, Hoda Samir Ghaith, Doaa Mohammad |
| description | Background
Searching for a non-antibiotic therapeutic option such as probiotics is gaining momentum nowadays. We aimed to evaluate the in vitro antibacterial ability of cell-free supernatant (CFS) of selected
Lactobacillus
strains (with probiotic properties) against clinical isolates of OXA-48-producing multidrug-resistant (MDR)
Klebsiella pneumoniae
separately and in combination with cefoperazone antibiotic.
Methods
Over a period of 8 months, a cross-sectional experimental study involving 590
Klebsiella pneumoniae
isolates was done. Our study took place at The Specialized Pediatric Teaching Hospital of Cairo University. Of the 590
Klebsiella pneumoniae
isolates collected from blood cultures, pus, endotracheal aspirates, and pleural fluid, only 50 unrepeated clinical isolates of MDR
Klebsiella pneumoniae-
producing OXA-48-like detected by CHROMID® OXA-48 (bioMérieux, France) were selected for our study. After determining the minimal inhibitory concentration of CFS of ten
Lactobacillus
strains and cefoperazone each, the synergistic effect of both was tested.
Results
Among ten tested
Lactobacillus
spp., a significant increase in the mean value of inhibition zone diameter with CFS of
L. helveticus
(14.32 mm) and
L. rhamnosus
(13.3 mm) was detected separately. On the contrary, an antagonistic activity against all tested isolates was detected upon combination of
Lactobacilli
with cefoperazone (512 μg/ml). The mean value of inhibition zone diameter of
L. helveticus
CFS+ cefoperazone was (11.0 mm) and for
L. rhamnosus
CFS+ cefoperazone was (10.88 mm) (
p
value |
| doi_str_mv | 10.1186/s42506-022-00114-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9548457</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_3629f3b86bbe40fb85c5a9f9864e5196</doaj_id><sourcerecordid>2723487647</sourcerecordid><originalsourceid>FETCH-LOGICAL-c517t-2afa170f181fc6e83cb4278ac1aeaff128efa5ed5a10bab99b50c5d98352f1f83</originalsourceid><addsrcrecordid>eNp9ksGO1SAUhhujiZNxXsAViRsXUwXaUroxMTej3jiJG03ckQM9VG5aqEAnmQeZ95U7d6KOC9lA4DsfcPJX1UtG3zAmxdvU8o6KmnJeU8pYW7dPqjNOB1pzwb8__Wv9vLpI6UDLELxvhDir7vae3LgcAwGfnQaTMTqYCVqLJpNgyRqDdiE7kwhM4HzKZAdRw4oeFkhYF2DcjPMTWbY5uzFuUx0xuZSLknyeUSeH8wxk9bgtwTtAYmbnnSn3uBRmyJgui9TFcEmupts1v6ieWZgTXjzM59W3D1dfd5_q6y8f97v317XpWJ9rDhZYTy2TzBqBsjG65b0EwwDBWsYlWuhw7IBRDXoYdEdNNw6y6bhlVjbn1f7kHQMc1BrdAvFWBXDqfiPESUEsX59RNYIPttFSaI0ttVp2poPBDlK02LFBFNe7k2vd9IKjQZ8jzI-kj0-8-6GmcKOGrpVt1xfB6wdBDD83TFktLplj5zyGLSne86aVvWiP6Kt_0EPYoi-tOlJ86IVkR4qfKBNDShHt78cwqo7JUafkqJIcdZ8c1Zai5lSUCuwnjH_U_6n6Bfw0y2I</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2722976817</pqid></control><display><type>article</type><title>In vitro antibacterial effect of probiotics against Carbapenamase-producing multidrug-resistant Klebsiella pneumoniae clinical isolates, Cairo, Egypt</title><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central Open Access</source><source>Springer Nature OA Free Journals</source><source>PubMed Central</source><source>SpringerLink Journals - AutoHoldings</source><creator>Abdelhalim, Mona Mohiedden ; Saafan, Ghada Samy ; El-Sayed, Hoda Samir ; Ghaith, Doaa Mohammad</creator><creatorcontrib>Abdelhalim, Mona Mohiedden ; Saafan, Ghada Samy ; El-Sayed, Hoda Samir ; Ghaith, Doaa Mohammad</creatorcontrib><description>Background
Searching for a non-antibiotic therapeutic option such as probiotics is gaining momentum nowadays. We aimed to evaluate the in vitro antibacterial ability of cell-free supernatant (CFS) of selected
Lactobacillus
strains (with probiotic properties) against clinical isolates of OXA-48-producing multidrug-resistant (MDR)
Klebsiella pneumoniae
separately and in combination with cefoperazone antibiotic.
Methods
Over a period of 8 months, a cross-sectional experimental study involving 590
Klebsiella pneumoniae
isolates was done. Our study took place at The Specialized Pediatric Teaching Hospital of Cairo University. Of the 590
Klebsiella pneumoniae
isolates collected from blood cultures, pus, endotracheal aspirates, and pleural fluid, only 50 unrepeated clinical isolates of MDR
Klebsiella pneumoniae-
producing OXA-48-like detected by CHROMID® OXA-48 (bioMérieux, France) were selected for our study. After determining the minimal inhibitory concentration of CFS of ten
Lactobacillus
strains and cefoperazone each, the synergistic effect of both was tested.
Results
Among ten tested
Lactobacillus
spp., a significant increase in the mean value of inhibition zone diameter with CFS of
L. helveticus
(14.32 mm) and
L. rhamnosus
(13.3 mm) was detected separately. On the contrary, an antagonistic activity against all tested isolates was detected upon combination of
Lactobacilli
with cefoperazone (512 μg/ml). The mean value of inhibition zone diameter of
L. helveticus
CFS+ cefoperazone was (11.0 mm) and for
L. rhamnosus
CFS+ cefoperazone was (10.88 mm) (
p
value <0.001).
Conclusion
The antimicrobial efficiency of using CFS of
Lactobacillus
species separately indicates that these therapies may be a substitute treatment strategy against MDR
Klebsiella pneumoniae
.</description><identifier>ISSN: 2090-262X</identifier><identifier>ISSN: 0013-2446</identifier><identifier>EISSN: 2090-262X</identifier><identifier>DOI: 10.1186/s42506-022-00114-4</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Antibiotics ; Bacterial infections ; CRE bacteria ; Drug resistance ; Lactobacillus spp ; MDR-Klebsiella pneumoniae ; Medical treatment ; Medicine ; Medicine & Public Health ; Multidrug resistant organisms ; OXA-48 ; Probiotics ; Public Health</subject><ispartof>Journal of the Egyptian Public Health Association, 2022-10, Vol.97 (1), p.19-19, Article 19</ispartof><rights>The Author(s) 2022</rights><rights>The Author(s) 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-2afa170f181fc6e83cb4278ac1aeaff128efa5ed5a10bab99b50c5d98352f1f83</citedby><cites>FETCH-LOGICAL-c517t-2afa170f181fc6e83cb4278ac1aeaff128efa5ed5a10bab99b50c5d98352f1f83</cites><orcidid>0000-0002-0846-063X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9548457/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9548457/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Abdelhalim, Mona Mohiedden</creatorcontrib><creatorcontrib>Saafan, Ghada Samy</creatorcontrib><creatorcontrib>El-Sayed, Hoda Samir</creatorcontrib><creatorcontrib>Ghaith, Doaa Mohammad</creatorcontrib><title>In vitro antibacterial effect of probiotics against Carbapenamase-producing multidrug-resistant Klebsiella pneumoniae clinical isolates, Cairo, Egypt</title><title>Journal of the Egyptian Public Health Association</title><addtitle>J. Egypt. Public. Health. Assoc</addtitle><description>Background
Searching for a non-antibiotic therapeutic option such as probiotics is gaining momentum nowadays. We aimed to evaluate the in vitro antibacterial ability of cell-free supernatant (CFS) of selected
Lactobacillus
strains (with probiotic properties) against clinical isolates of OXA-48-producing multidrug-resistant (MDR)
Klebsiella pneumoniae
separately and in combination with cefoperazone antibiotic.
Methods
Over a period of 8 months, a cross-sectional experimental study involving 590
Klebsiella pneumoniae
isolates was done. Our study took place at The Specialized Pediatric Teaching Hospital of Cairo University. Of the 590
Klebsiella pneumoniae
isolates collected from blood cultures, pus, endotracheal aspirates, and pleural fluid, only 50 unrepeated clinical isolates of MDR
Klebsiella pneumoniae-
producing OXA-48-like detected by CHROMID® OXA-48 (bioMérieux, France) were selected for our study. After determining the minimal inhibitory concentration of CFS of ten
Lactobacillus
strains and cefoperazone each, the synergistic effect of both was tested.
Results
Among ten tested
Lactobacillus
spp., a significant increase in the mean value of inhibition zone diameter with CFS of
L. helveticus
(14.32 mm) and
L. rhamnosus
(13.3 mm) was detected separately. On the contrary, an antagonistic activity against all tested isolates was detected upon combination of
Lactobacilli
with cefoperazone (512 μg/ml). The mean value of inhibition zone diameter of
L. helveticus
CFS+ cefoperazone was (11.0 mm) and for
L. rhamnosus
CFS+ cefoperazone was (10.88 mm) (
p
value <0.001).
Conclusion
The antimicrobial efficiency of using CFS of
Lactobacillus
species separately indicates that these therapies may be a substitute treatment strategy against MDR
Klebsiella pneumoniae
.</description><subject>Antibiotics</subject><subject>Bacterial infections</subject><subject>CRE bacteria</subject><subject>Drug resistance</subject><subject>Lactobacillus spp</subject><subject>MDR-Klebsiella pneumoniae</subject><subject>Medical treatment</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Multidrug resistant organisms</subject><subject>OXA-48</subject><subject>Probiotics</subject><subject>Public Health</subject><issn>2090-262X</issn><issn>0013-2446</issn><issn>2090-262X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNp9ksGO1SAUhhujiZNxXsAViRsXUwXaUroxMTej3jiJG03ckQM9VG5aqEAnmQeZ95U7d6KOC9lA4DsfcPJX1UtG3zAmxdvU8o6KmnJeU8pYW7dPqjNOB1pzwb8__Wv9vLpI6UDLELxvhDir7vae3LgcAwGfnQaTMTqYCVqLJpNgyRqDdiE7kwhM4HzKZAdRw4oeFkhYF2DcjPMTWbY5uzFuUx0xuZSLknyeUSeH8wxk9bgtwTtAYmbnnSn3uBRmyJgui9TFcEmupts1v6ieWZgTXjzM59W3D1dfd5_q6y8f97v317XpWJ9rDhZYTy2TzBqBsjG65b0EwwDBWsYlWuhw7IBRDXoYdEdNNw6y6bhlVjbn1f7kHQMc1BrdAvFWBXDqfiPESUEsX59RNYIPttFSaI0ttVp2poPBDlK02LFBFNe7k2vd9IKjQZ8jzI-kj0-8-6GmcKOGrpVt1xfB6wdBDD83TFktLplj5zyGLSne86aVvWiP6Kt_0EPYoi-tOlJ86IVkR4qfKBNDShHt78cwqo7JUafkqJIcdZ8c1Zai5lSUCuwnjH_U_6n6Bfw0y2I</recordid><startdate>20221010</startdate><enddate>20221010</enddate><creator>Abdelhalim, Mona Mohiedden</creator><creator>Saafan, Ghada Samy</creator><creator>El-Sayed, Hoda Samir</creator><creator>Ghaith, Doaa Mohammad</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><general>SpringerOpen</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8C1</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-0846-063X</orcidid></search><sort><creationdate>20221010</creationdate><title>In vitro antibacterial effect of probiotics against Carbapenamase-producing multidrug-resistant Klebsiella pneumoniae clinical isolates, Cairo, Egypt</title><author>Abdelhalim, Mona Mohiedden ; Saafan, Ghada Samy ; El-Sayed, Hoda Samir ; Ghaith, Doaa Mohammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-2afa170f181fc6e83cb4278ac1aeaff128efa5ed5a10bab99b50c5d98352f1f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antibiotics</topic><topic>Bacterial infections</topic><topic>CRE bacteria</topic><topic>Drug resistance</topic><topic>Lactobacillus spp</topic><topic>MDR-Klebsiella pneumoniae</topic><topic>Medical treatment</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Multidrug resistant organisms</topic><topic>OXA-48</topic><topic>Probiotics</topic><topic>Public Health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abdelhalim, Mona Mohiedden</creatorcontrib><creatorcontrib>Saafan, Ghada Samy</creatorcontrib><creatorcontrib>El-Sayed, Hoda Samir</creatorcontrib><creatorcontrib>Ghaith, Doaa Mohammad</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>Public Health Database</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of the Egyptian Public Health Association</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abdelhalim, Mona Mohiedden</au><au>Saafan, Ghada Samy</au><au>El-Sayed, Hoda Samir</au><au>Ghaith, Doaa Mohammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro antibacterial effect of probiotics against Carbapenamase-producing multidrug-resistant Klebsiella pneumoniae clinical isolates, Cairo, Egypt</atitle><jtitle>Journal of the Egyptian Public Health Association</jtitle><stitle>J. Egypt. Public. Health. Assoc</stitle><date>2022-10-10</date><risdate>2022</risdate><volume>97</volume><issue>1</issue><spage>19</spage><epage>19</epage><pages>19-19</pages><artnum>19</artnum><issn>2090-262X</issn><issn>0013-2446</issn><eissn>2090-262X</eissn><abstract>Background
Searching for a non-antibiotic therapeutic option such as probiotics is gaining momentum nowadays. We aimed to evaluate the in vitro antibacterial ability of cell-free supernatant (CFS) of selected
Lactobacillus
strains (with probiotic properties) against clinical isolates of OXA-48-producing multidrug-resistant (MDR)
Klebsiella pneumoniae
separately and in combination with cefoperazone antibiotic.
Methods
Over a period of 8 months, a cross-sectional experimental study involving 590
Klebsiella pneumoniae
isolates was done. Our study took place at The Specialized Pediatric Teaching Hospital of Cairo University. Of the 590
Klebsiella pneumoniae
isolates collected from blood cultures, pus, endotracheal aspirates, and pleural fluid, only 50 unrepeated clinical isolates of MDR
Klebsiella pneumoniae-
producing OXA-48-like detected by CHROMID® OXA-48 (bioMérieux, France) were selected for our study. After determining the minimal inhibitory concentration of CFS of ten
Lactobacillus
strains and cefoperazone each, the synergistic effect of both was tested.
Results
Among ten tested
Lactobacillus
spp., a significant increase in the mean value of inhibition zone diameter with CFS of
L. helveticus
(14.32 mm) and
L. rhamnosus
(13.3 mm) was detected separately. On the contrary, an antagonistic activity against all tested isolates was detected upon combination of
Lactobacilli
with cefoperazone (512 μg/ml). The mean value of inhibition zone diameter of
L. helveticus
CFS+ cefoperazone was (11.0 mm) and for
L. rhamnosus
CFS+ cefoperazone was (10.88 mm) (
p
value <0.001).
Conclusion
The antimicrobial efficiency of using CFS of
Lactobacillus
species separately indicates that these therapies may be a substitute treatment strategy against MDR
Klebsiella pneumoniae
.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1186/s42506-022-00114-4</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-0846-063X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; Springer Nature OA Free Journals; PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Antibiotics Bacterial infections CRE bacteria Drug resistance Lactobacillus spp MDR-Klebsiella pneumoniae Medical treatment Medicine Medicine & Public Health Multidrug resistant organisms OXA-48 Probiotics Public Health |
| title | In vitro antibacterial effect of probiotics against Carbapenamase-producing multidrug-resistant Klebsiella pneumoniae clinical isolates, Cairo, Egypt |
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