The Brucella suis homologue of the Agrobacterium tumefaciens chromosomal virulence operon chvE is essential for sugar utilization but not for survival in macrophages
Brucella strains possess an operon encoding type IV secretion machinery very similar to that coded by the Agrobacterium tumefaciens virB operon. Here we describe cloning of the Brucella suis homologue of the chvE-gguA-gguB operon of A. tumefaciens and characterize the sugar binding protein ChvE (78%...
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| Veröffentlicht in: | Journal of bacteriology 2001-09, Vol.183 (18), p.5343-5351 |
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| creator | Alvarez-Martinez, M T Machold, J Weise, C Schmidt-Eisenlohr, H Baron, C Rouot, B |
| description | Brucella strains possess an operon encoding type IV secretion machinery very similar to that coded by the Agrobacterium tumefaciens virB operon. Here we describe cloning of the Brucella suis homologue of the chvE-gguA-gguB operon of A. tumefaciens and characterize the sugar binding protein ChvE (78% identity), which in A. tumefaciens is involved in virulence gene expression. B. suis chvE is upstream of the putative sugar transporter-encoding genes gguA and gguB, also present in A. tumefaciens, but not adjacent to that of a LysR-type transcription regulator. Although results of Southern hybridization experiments suggested that the gene is present in all Brucella strains, the ChvE protein was detected only in B. suis and Brucella canis with A. tumefaciens ChvE-specific antisera, suggesting that chvE genes are differently expressed in different Brucella species. Analysis of cell growth of B. suis and of its chvE or gguA mutants in different media revealed that ChvE exhibited a sugar specificity similar to that of its A. tumefaciens homologue and that both ChvE and GguA were necessary for utilization of these sugars. Murine or human macrophage infections with B. suis chvE and gguA mutants resulted in multiplication similar to that of the wild-type strain, suggesting that virB expression was unaffected. These data indicate that the ChvE and GguA homologous proteins of B. suis are essential for the utilization of certain sugars but are not necessary for survival and replication inside macrophages. |
| doi_str_mv | 10.1128/JB.183.18.5343-5351.2001 |
| format | Article |
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Here we describe cloning of the Brucella suis homologue of the chvE-gguA-gguB operon of A. tumefaciens and characterize the sugar binding protein ChvE (78% identity), which in A. tumefaciens is involved in virulence gene expression. B. suis chvE is upstream of the putative sugar transporter-encoding genes gguA and gguB, also present in A. tumefaciens, but not adjacent to that of a LysR-type transcription regulator. Although results of Southern hybridization experiments suggested that the gene is present in all Brucella strains, the ChvE protein was detected only in B. suis and Brucella canis with A. tumefaciens ChvE-specific antisera, suggesting that chvE genes are differently expressed in different Brucella species. Analysis of cell growth of B. suis and of its chvE or gguA mutants in different media revealed that ChvE exhibited a sugar specificity similar to that of its A. tumefaciens homologue and that both ChvE and GguA were necessary for utilization of these sugars. Murine or human macrophage infections with B. suis chvE and gguA mutants resulted in multiplication similar to that of the wild-type strain, suggesting that virB expression was unaffected. These data indicate that the ChvE and GguA homologous proteins of B. suis are essential for the utilization of certain sugars but are not necessary for survival and replication inside macrophages.</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>DOI: 10.1128/JB.183.18.5343-5351.2001</identifier><identifier>PMID: 11514518</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Agrobacterium tumefaciens ; Agrobacterium tumefaciens - genetics ; Amino Acid Sequence ; Animals ; ATP-Binding Cassette Transporters - genetics ; ATP-Binding Cassette Transporters - metabolism ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; Brucella - genetics ; Brucella - growth & development ; Brucella - pathogenicity ; Brucella suis ; Brucellosis - microbiology ; chvE gene ; ChvE protein ; Cloning, Molecular ; Gene Deletion ; Gene Expression Regulation, Bacterial ; Genes ; Genetic Complementation Test ; Genetics and Molecular Biology ; gguA gene ; GguA protein ; gguB gene ; Humans ; Macrophages - microbiology ; Membrane Transport Proteins ; Mice ; Molecular Sequence Data ; Monosaccharide Transport Proteins - genetics ; Monosaccharide Transport Proteins - metabolism ; Monosaccharides - metabolism ; Pathogens ; Periplasmic Binding Proteins ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Sugar ; virB gene ; Virulence</subject><ispartof>Journal of bacteriology, 2001-09, Vol.183 (18), p.5343-5351</ispartof><rights>Copyright American Society for Microbiology Sep 2001</rights><rights>Copyright © 2001, American Society for Microbiology 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-f61a3d9434843465ba5a5133aecc309d60cdc844cd3df6fbcee1ba6acbcb31833</citedby><cites>FETCH-LOGICAL-c472t-f61a3d9434843465ba5a5133aecc309d60cdc844cd3df6fbcee1ba6acbcb31833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC95417/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC95417/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11514518$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alvarez-Martinez, M T</creatorcontrib><creatorcontrib>Machold, J</creatorcontrib><creatorcontrib>Weise, C</creatorcontrib><creatorcontrib>Schmidt-Eisenlohr, H</creatorcontrib><creatorcontrib>Baron, C</creatorcontrib><creatorcontrib>Rouot, B</creatorcontrib><title>The Brucella suis homologue of the Agrobacterium tumefaciens chromosomal virulence operon chvE is essential for sugar utilization but not for survival in macrophages</title><title>Journal of bacteriology</title><addtitle>J Bacteriol</addtitle><description>Brucella strains possess an operon encoding type IV secretion machinery very similar to that coded by the Agrobacterium tumefaciens virB operon. Here we describe cloning of the Brucella suis homologue of the chvE-gguA-gguB operon of A. tumefaciens and characterize the sugar binding protein ChvE (78% identity), which in A. tumefaciens is involved in virulence gene expression. B. suis chvE is upstream of the putative sugar transporter-encoding genes gguA and gguB, also present in A. tumefaciens, but not adjacent to that of a LysR-type transcription regulator. Although results of Southern hybridization experiments suggested that the gene is present in all Brucella strains, the ChvE protein was detected only in B. suis and Brucella canis with A. tumefaciens ChvE-specific antisera, suggesting that chvE genes are differently expressed in different Brucella species. Analysis of cell growth of B. suis and of its chvE or gguA mutants in different media revealed that ChvE exhibited a sugar specificity similar to that of its A. tumefaciens homologue and that both ChvE and GguA were necessary for utilization of these sugars. Murine or human macrophage infections with B. suis chvE and gguA mutants resulted in multiplication similar to that of the wild-type strain, suggesting that virB expression was unaffected. These data indicate that the ChvE and GguA homologous proteins of B. suis are essential for the utilization of certain sugars but are not necessary for survival and replication inside macrophages.</description><subject>Agrobacterium tumefaciens</subject><subject>Agrobacterium tumefaciens - genetics</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>ATP-Binding Cassette Transporters - metabolism</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Brucella - genetics</subject><subject>Brucella - growth & development</subject><subject>Brucella - pathogenicity</subject><subject>Brucella suis</subject><subject>Brucellosis - microbiology</subject><subject>chvE gene</subject><subject>ChvE protein</subject><subject>Cloning, Molecular</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes</subject><subject>Genetic Complementation Test</subject><subject>Genetics and Molecular Biology</subject><subject>gguA gene</subject><subject>GguA protein</subject><subject>gguB gene</subject><subject>Humans</subject><subject>Macrophages - microbiology</subject><subject>Membrane Transport Proteins</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Monosaccharide Transport Proteins - genetics</subject><subject>Monosaccharide Transport Proteins - metabolism</subject><subject>Monosaccharides - metabolism</subject><subject>Pathogens</subject><subject>Periplasmic Binding Proteins</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sugar</subject><subject>virB gene</subject><subject>Virulence</subject><issn>0021-9193</issn><issn>1098-5530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkstu1TAQhi0EoofCKyCLBbscPLGdi8Smp2qBqhKbsrYcx0lcJfHBjiOV9-E9O6FH3BYjL-b75-afEApsD5BXH24Oe6g4xl5ywTPJJexzxuAZ2QGrq0xKzp6THWM5ZDXU_Iy8ivEeASFk_pKcAUgQEqod-Xk3WHoIydhx1DQmF-ngJz_6PlnqO7pg-qIPvtFmscGliS5psp02zs6RmiEgHP2kR7q6kEY7G5QdbfAzJtcrivVsjHZeHCKdD9ii14GmxY3uh14cck1a6OyXUzasbkXUzXTSJvjjoHsbX5MXnR6jfXN6z8m366u7y8_Z7ddPXy4vbjMjynzJugI0b2vBRYVRyEZLLYFzbY3hrG4LZlpTCWFa3nZF1xhrodGFNo1pOB6Un5OPT3WPqZlsa3DuoEd1DG7S4UF57dS_mdkNqverqqWAEuXvT_LgvycbFzW5-Ouys_UpKqigLEBufd79B977FGZcTeV5yeq8LDeoeoLwDDEG2_2eA5jabKBuDliSY6jNBmqzgdpsgNK3f-_xR3j6d_4IoTS1Cg</recordid><startdate>20010901</startdate><enddate>20010901</enddate><creator>Alvarez-Martinez, M T</creator><creator>Machold, J</creator><creator>Weise, C</creator><creator>Schmidt-Eisenlohr, H</creator><creator>Baron, C</creator><creator>Rouot, B</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20010901</creationdate><title>The Brucella suis homologue of the Agrobacterium tumefaciens chromosomal virulence operon chvE is essential for sugar utilization but not for survival in macrophages</title><author>Alvarez-Martinez, M T ; Machold, J ; Weise, C ; Schmidt-Eisenlohr, H ; Baron, C ; Rouot, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-f61a3d9434843465ba5a5133aecc309d60cdc844cd3df6fbcee1ba6acbcb31833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Agrobacterium tumefaciens</topic><topic>Agrobacterium tumefaciens - genetics</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>ATP-Binding Cassette Transporters - genetics</topic><topic>ATP-Binding Cassette Transporters - metabolism</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Brucella - genetics</topic><topic>Brucella - growth & development</topic><topic>Brucella - pathogenicity</topic><topic>Brucella suis</topic><topic>Brucellosis - microbiology</topic><topic>chvE gene</topic><topic>ChvE protein</topic><topic>Cloning, Molecular</topic><topic>Gene Deletion</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes</topic><topic>Genetic Complementation Test</topic><topic>Genetics and Molecular Biology</topic><topic>gguA gene</topic><topic>GguA protein</topic><topic>gguB gene</topic><topic>Humans</topic><topic>Macrophages - microbiology</topic><topic>Membrane Transport Proteins</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Monosaccharide Transport Proteins - genetics</topic><topic>Monosaccharide Transport Proteins - metabolism</topic><topic>Monosaccharides - metabolism</topic><topic>Pathogens</topic><topic>Periplasmic Binding Proteins</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sugar</topic><topic>virB gene</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alvarez-Martinez, M T</creatorcontrib><creatorcontrib>Machold, J</creatorcontrib><creatorcontrib>Weise, C</creatorcontrib><creatorcontrib>Schmidt-Eisenlohr, H</creatorcontrib><creatorcontrib>Baron, C</creatorcontrib><creatorcontrib>Rouot, B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alvarez-Martinez, M T</au><au>Machold, J</au><au>Weise, C</au><au>Schmidt-Eisenlohr, H</au><au>Baron, C</au><au>Rouot, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Brucella suis homologue of the Agrobacterium tumefaciens chromosomal virulence operon chvE is essential for sugar utilization but not for survival in macrophages</atitle><jtitle>Journal of bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>2001-09-01</date><risdate>2001</risdate><volume>183</volume><issue>18</issue><spage>5343</spage><epage>5351</epage><pages>5343-5351</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><coden>JOBAAY</coden><abstract>Brucella strains possess an operon encoding type IV secretion machinery very similar to that coded by the Agrobacterium tumefaciens virB operon. Here we describe cloning of the Brucella suis homologue of the chvE-gguA-gguB operon of A. tumefaciens and characterize the sugar binding protein ChvE (78% identity), which in A. tumefaciens is involved in virulence gene expression. B. suis chvE is upstream of the putative sugar transporter-encoding genes gguA and gguB, also present in A. tumefaciens, but not adjacent to that of a LysR-type transcription regulator. Although results of Southern hybridization experiments suggested that the gene is present in all Brucella strains, the ChvE protein was detected only in B. suis and Brucella canis with A. tumefaciens ChvE-specific antisera, suggesting that chvE genes are differently expressed in different Brucella species. Analysis of cell growth of B. suis and of its chvE or gguA mutants in different media revealed that ChvE exhibited a sugar specificity similar to that of its A. tumefaciens homologue and that both ChvE and GguA were necessary for utilization of these sugars. Murine or human macrophage infections with B. suis chvE and gguA mutants resulted in multiplication similar to that of the wild-type strain, suggesting that virB expression was unaffected. These data indicate that the ChvE and GguA homologous proteins of B. suis are essential for the utilization of certain sugars but are not necessary for survival and replication inside macrophages.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>11514518</pmid><doi>10.1128/JB.183.18.5343-5351.2001</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Agrobacterium tumefaciens Agrobacterium tumefaciens - genetics Amino Acid Sequence Animals ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Brucella - genetics Brucella - growth & development Brucella - pathogenicity Brucella suis Brucellosis - microbiology chvE gene ChvE protein Cloning, Molecular Gene Deletion Gene Expression Regulation, Bacterial Genes Genetic Complementation Test Genetics and Molecular Biology gguA gene GguA protein gguB gene Humans Macrophages - microbiology Membrane Transport Proteins Mice Molecular Sequence Data Monosaccharide Transport Proteins - genetics Monosaccharide Transport Proteins - metabolism Monosaccharides - metabolism Pathogens Periplasmic Binding Proteins Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Sugar virB gene Virulence |
| title | The Brucella suis homologue of the Agrobacterium tumefaciens chromosomal virulence operon chvE is essential for sugar utilization but not for survival in macrophages |
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