The immunological role of B7‐H4 in pregnant women with Sars‐Cov2 infection
Problem T‐cells are key players in fighting the coronavirus disease 2019 (COVID‐19). The checkpoint molecule B7‐H4, a member of the B7 family, can inhibit T‐cell activation and proliferation by inhibiting NF‐kb expression. We aimed to elucidate the immunological role of soluble B7‐H4 (sB7‐H4) and B7...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 2022-12, Vol.88 (6), p.e13626-n/a |
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| container_title | American journal of reproductive immunology (1989) |
| container_volume | 88 |
| creator | Duan, Liyan Reisch, Beatrix Mach, Pawel Kimmig, Rainer Gellhaus, Alexandra Iannaccone, Antonella |
| description | Problem
T‐cells are key players in fighting the coronavirus disease 2019 (COVID‐19). The checkpoint molecule B7‐H4, a member of the B7 family, can inhibit T‐cell activation and proliferation by inhibiting NF‐kb expression. We aimed to elucidate the immunological role of soluble B7‐H4 (sB7‐H4) and B7‐H4 in pregnant women suffered from an acute Sars‐Cov2 infection.
Methods
Expression levels of sB7‐H4 and cytokines were detected by enzyme linked immunosorbent assay. B7‐H4 and cytokines mRNA expression was analyzed by qPCR, and B7‐H4 and NF‐κb (p65) protein levels were investigated by western blot and immunofluorescence staining in placenta chorionic villous and decidual basalis tissues of COVID‐19 affected women and healthy controls.
Results
Fibrinoid necrosis in the periphery of placental villi was increased in the COVID‐19‐affected patients. sB7‐H4 protein in maternal and cord blood serum and IL‐6/IL‐10 were increased while leukocytes were decreased during SARS‐CoV‐2 infection. Serum sB7‐H4 level was increased according to the severity of SARS‐Cov‐2 infection. Cytokines (IL‐6, IL‐18, IL‐1β, TNF‐α), B7‐H4 mRNA and protein in the decidual basalis tissues of COVID‐19‐infected pregnant women were significantly increased compared to healthy controls. IL‐18 and IL‐1β were significantly increased in the placenta chorionic villous samples of COVID‐19 affected patients, while NF‐κb (p65) expression was decreased.
Conclusions
The expression of the immunological marker sB7‐H4 correlated with the severity of COVID‐19 disease in pregnant women. sB7‐H4 and B7‐H4 can be used to monitor the progression of COVID‐19 infection during pregnancy, and for evaluating of the maternal immune status. |
| doi_str_mv | 10.1111/aji.13626 |
| format | Article |
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T‐cells are key players in fighting the coronavirus disease 2019 (COVID‐19). The checkpoint molecule B7‐H4, a member of the B7 family, can inhibit T‐cell activation and proliferation by inhibiting NF‐kb expression. We aimed to elucidate the immunological role of soluble B7‐H4 (sB7‐H4) and B7‐H4 in pregnant women suffered from an acute Sars‐Cov2 infection.
Methods
Expression levels of sB7‐H4 and cytokines were detected by enzyme linked immunosorbent assay. B7‐H4 and cytokines mRNA expression was analyzed by qPCR, and B7‐H4 and NF‐κb (p65) protein levels were investigated by western blot and immunofluorescence staining in placenta chorionic villous and decidual basalis tissues of COVID‐19 affected women and healthy controls.
Results
Fibrinoid necrosis in the periphery of placental villi was increased in the COVID‐19‐affected patients. sB7‐H4 protein in maternal and cord blood serum and IL‐6/IL‐10 were increased while leukocytes were decreased during SARS‐CoV‐2 infection. Serum sB7‐H4 level was increased according to the severity of SARS‐Cov‐2 infection. Cytokines (IL‐6, IL‐18, IL‐1β, TNF‐α), B7‐H4 mRNA and protein in the decidual basalis tissues of COVID‐19‐infected pregnant women were significantly increased compared to healthy controls. IL‐18 and IL‐1β were significantly increased in the placenta chorionic villous samples of COVID‐19 affected patients, while NF‐κb (p65) expression was decreased.
Conclusions
The expression of the immunological marker sB7‐H4 correlated with the severity of COVID‐19 disease in pregnant women. sB7‐H4 and B7‐H4 can be used to monitor the progression of COVID‐19 infection during pregnancy, and for evaluating of the maternal immune status.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/aji.13626</identifier><identifier>PMID: 36121927</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>B7‐H4 ; Cell activation ; Cord blood ; Coronaviruses ; COVID-19 ; Cytokines ; Decidua ; Enzyme-linked immunosorbent assay ; Female ; Gene expression ; Humans ; immune regulation ; Immune status ; Immunofluorescence ; Immunology ; infection ; Infections ; Interleukin 6 ; Interleukin-18 ; Leukocytes ; NF-kappa B ; Original ; Placenta ; Pregnancy ; Pregnant Women ; Proteins ; RNA, Messenger ; RNA, Viral ; SARS-CoV-2 ; SARS‐CoV‐2 T‐cells ; Severe acute respiratory syndrome coronavirus 2 ; Tumor necrosis factor ; V-Set Domain-Containing T-Cell Activation Inhibitor 1 ; Womens health</subject><ispartof>American journal of reproductive immunology (1989), 2022-12, Vol.88 (6), p.e13626-n/a</ispartof><rights>2022 The Authors. published by John Wiley & Sons Ltd.</rights><rights>2022 The Authors. American Journal of Reproductive Immunology published by John Wiley & Sons Ltd.</rights><rights>2022. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4436-1970963fd774814081588ac9d5607c34c6a4cbeb3030a19311a659c928e4809e3</citedby><cites>FETCH-LOGICAL-c4436-1970963fd774814081588ac9d5607c34c6a4cbeb3030a19311a659c928e4809e3</cites><orcidid>0000-0002-7301-6746</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faji.13626$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faji.13626$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36121927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duan, Liyan</creatorcontrib><creatorcontrib>Reisch, Beatrix</creatorcontrib><creatorcontrib>Mach, Pawel</creatorcontrib><creatorcontrib>Kimmig, Rainer</creatorcontrib><creatorcontrib>Gellhaus, Alexandra</creatorcontrib><creatorcontrib>Iannaccone, Antonella</creatorcontrib><title>The immunological role of B7‐H4 in pregnant women with Sars‐Cov2 infection</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Problem
T‐cells are key players in fighting the coronavirus disease 2019 (COVID‐19). The checkpoint molecule B7‐H4, a member of the B7 family, can inhibit T‐cell activation and proliferation by inhibiting NF‐kb expression. We aimed to elucidate the immunological role of soluble B7‐H4 (sB7‐H4) and B7‐H4 in pregnant women suffered from an acute Sars‐Cov2 infection.
Methods
Expression levels of sB7‐H4 and cytokines were detected by enzyme linked immunosorbent assay. B7‐H4 and cytokines mRNA expression was analyzed by qPCR, and B7‐H4 and NF‐κb (p65) protein levels were investigated by western blot and immunofluorescence staining in placenta chorionic villous and decidual basalis tissues of COVID‐19 affected women and healthy controls.
Results
Fibrinoid necrosis in the periphery of placental villi was increased in the COVID‐19‐affected patients. sB7‐H4 protein in maternal and cord blood serum and IL‐6/IL‐10 were increased while leukocytes were decreased during SARS‐CoV‐2 infection. Serum sB7‐H4 level was increased according to the severity of SARS‐Cov‐2 infection. Cytokines (IL‐6, IL‐18, IL‐1β, TNF‐α), B7‐H4 mRNA and protein in the decidual basalis tissues of COVID‐19‐infected pregnant women were significantly increased compared to healthy controls. IL‐18 and IL‐1β were significantly increased in the placenta chorionic villous samples of COVID‐19 affected patients, while NF‐κb (p65) expression was decreased.
Conclusions
The expression of the immunological marker sB7‐H4 correlated with the severity of COVID‐19 disease in pregnant women. sB7‐H4 and B7‐H4 can be used to monitor the progression of COVID‐19 infection during pregnancy, and for evaluating of the maternal immune status.</description><subject>B7‐H4</subject><subject>Cell activation</subject><subject>Cord blood</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>Cytokines</subject><subject>Decidua</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>Gene expression</subject><subject>Humans</subject><subject>immune regulation</subject><subject>Immune status</subject><subject>Immunofluorescence</subject><subject>Immunology</subject><subject>infection</subject><subject>Infections</subject><subject>Interleukin 6</subject><subject>Interleukin-18</subject><subject>Leukocytes</subject><subject>NF-kappa B</subject><subject>Original</subject><subject>Placenta</subject><subject>Pregnancy</subject><subject>Pregnant Women</subject><subject>Proteins</subject><subject>RNA, Messenger</subject><subject>RNA, Viral</subject><subject>SARS-CoV-2</subject><subject>SARS‐CoV‐2 T‐cells</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Tumor necrosis factor</subject><subject>V-Set Domain-Containing T-Cell Activation Inhibitor 1</subject><subject>Womens health</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>EIF</sourceid><recordid>eNp1kc1OJCEYRYmZif8LX8CQzGZclH4UFBSbSZyOfxOjC3VNaJrqplMFPVBlx52P4DP6JOK0mnGSYQOBk5P7cRHaI3BI8jrSc3dIKC_5GtokHKCAWoov-QyMF4JBvYG2UpoD5Hsq1tEG5aQkshSb6Op2ZrHrusGHNkyd0S2OobU4NPineH58OmfYebyIduq17_EydNbjpetn-EbHlIFRuC8z0ljTu-B30NdGt8nuvu3b6O705HZ0Xlxen12Mji8LwxjlBZECJKfNRAhWkxyQVHWtjZxUHIShzHDNzNiOKVDQRFJCNK-kkWVtWQ3S0m30Y-VdDOPOToz1fdStWkTX6figgnbq84t3MzUN90pWtK6YyILvb4IYfg829apzydi21d6GIalS5I-UUEmZ0W__oPMwRJ_HyxQjIFglIFMHK8rEkFK0zUcYAuq1JZVbUn9ayuz-3-k_yPdaMnC0ApautQ__N6njXxcr5QtHvJt2</recordid><startdate>202212</startdate><enddate>202212</enddate><creator>Duan, Liyan</creator><creator>Reisch, Beatrix</creator><creator>Mach, Pawel</creator><creator>Kimmig, Rainer</creator><creator>Gellhaus, Alexandra</creator><creator>Iannaccone, Antonella</creator><general>Wiley Subscription Services, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7301-6746</orcidid></search><sort><creationdate>202212</creationdate><title>The immunological role of B7‐H4 in pregnant women with Sars‐Cov2 infection</title><author>Duan, Liyan ; Reisch, Beatrix ; Mach, Pawel ; Kimmig, Rainer ; Gellhaus, Alexandra ; Iannaccone, Antonella</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4436-1970963fd774814081588ac9d5607c34c6a4cbeb3030a19311a659c928e4809e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>B7‐H4</topic><topic>Cell activation</topic><topic>Cord blood</topic><topic>Coronaviruses</topic><topic>COVID-19</topic><topic>Cytokines</topic><topic>Decidua</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Female</topic><topic>Gene expression</topic><topic>Humans</topic><topic>immune regulation</topic><topic>Immune status</topic><topic>Immunofluorescence</topic><topic>Immunology</topic><topic>infection</topic><topic>Infections</topic><topic>Interleukin 6</topic><topic>Interleukin-18</topic><topic>Leukocytes</topic><topic>NF-kappa B</topic><topic>Original</topic><topic>Placenta</topic><topic>Pregnancy</topic><topic>Pregnant Women</topic><topic>Proteins</topic><topic>RNA, Messenger</topic><topic>RNA, Viral</topic><topic>SARS-CoV-2</topic><topic>SARS‐CoV‐2 T‐cells</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Tumor necrosis factor</topic><topic>V-Set Domain-Containing T-Cell Activation Inhibitor 1</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duan, Liyan</creatorcontrib><creatorcontrib>Reisch, Beatrix</creatorcontrib><creatorcontrib>Mach, Pawel</creatorcontrib><creatorcontrib>Kimmig, Rainer</creatorcontrib><creatorcontrib>Gellhaus, Alexandra</creatorcontrib><creatorcontrib>Iannaccone, Antonella</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duan, Liyan</au><au>Reisch, Beatrix</au><au>Mach, Pawel</au><au>Kimmig, Rainer</au><au>Gellhaus, Alexandra</au><au>Iannaccone, Antonella</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The immunological role of B7‐H4 in pregnant women with Sars‐Cov2 infection</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2022-12</date><risdate>2022</risdate><volume>88</volume><issue>6</issue><spage>e13626</spage><epage>n/a</epage><pages>e13626-n/a</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>Problem
T‐cells are key players in fighting the coronavirus disease 2019 (COVID‐19). The checkpoint molecule B7‐H4, a member of the B7 family, can inhibit T‐cell activation and proliferation by inhibiting NF‐kb expression. We aimed to elucidate the immunological role of soluble B7‐H4 (sB7‐H4) and B7‐H4 in pregnant women suffered from an acute Sars‐Cov2 infection.
Methods
Expression levels of sB7‐H4 and cytokines were detected by enzyme linked immunosorbent assay. B7‐H4 and cytokines mRNA expression was analyzed by qPCR, and B7‐H4 and NF‐κb (p65) protein levels were investigated by western blot and immunofluorescence staining in placenta chorionic villous and decidual basalis tissues of COVID‐19 affected women and healthy controls.
Results
Fibrinoid necrosis in the periphery of placental villi was increased in the COVID‐19‐affected patients. sB7‐H4 protein in maternal and cord blood serum and IL‐6/IL‐10 were increased while leukocytes were decreased during SARS‐CoV‐2 infection. Serum sB7‐H4 level was increased according to the severity of SARS‐Cov‐2 infection. Cytokines (IL‐6, IL‐18, IL‐1β, TNF‐α), B7‐H4 mRNA and protein in the decidual basalis tissues of COVID‐19‐infected pregnant women were significantly increased compared to healthy controls. IL‐18 and IL‐1β were significantly increased in the placenta chorionic villous samples of COVID‐19 affected patients, while NF‐κb (p65) expression was decreased.
Conclusions
The expression of the immunological marker sB7‐H4 correlated with the severity of COVID‐19 disease in pregnant women. sB7‐H4 and B7‐H4 can be used to monitor the progression of COVID‐19 infection during pregnancy, and for evaluating of the maternal immune status.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36121927</pmid><doi>10.1111/aji.13626</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-7301-6746</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1046-7408 |
| ispartof | American journal of reproductive immunology (1989), 2022-12, Vol.88 (6), p.e13626-n/a |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | B7‐H4 Cell activation Cord blood Coronaviruses COVID-19 Cytokines Decidua Enzyme-linked immunosorbent assay Female Gene expression Humans immune regulation Immune status Immunofluorescence Immunology infection Infections Interleukin 6 Interleukin-18 Leukocytes NF-kappa B Original Placenta Pregnancy Pregnant Women Proteins RNA, Messenger RNA, Viral SARS-CoV-2 SARS‐CoV‐2 T‐cells Severe acute respiratory syndrome coronavirus 2 Tumor necrosis factor V-Set Domain-Containing T-Cell Activation Inhibitor 1 Womens health |
| title | The immunological role of B7‐H4 in pregnant women with Sars‐Cov2 infection |
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