Fine mapping of Rf5 region for a sorghum fertility restorer gene and microsynteny analysis across grass species

Cytoplasmic male sterility (CMS) is widely used to control pollination in the production of commercial F1 hybrid seed in sorghum. So far, 6 major fertility restorer genes, Rf1 to Rf6, have been reported in sorghum. Here, we fine-mapped the Rf5 locus on sorghum chromosome 5 using descendant populatio...

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Veröffentlicht in:Breeding Science 2022, Vol.72(2), pp.141-149
Hauptverfasser: Kiyosawa, Atsushi, Yonemaru, Jun-ichi, Mizuno, Hiroshi, Kanamori, Hiroyuki, Wu, Jianzhong, Kawahigashi, Hiroyuki, Goto, Kazumi
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container_issue 2
container_start_page 141
container_title Breeding Science
container_volume 72
creator Kiyosawa, Atsushi
Yonemaru, Jun-ichi
Mizuno, Hiroshi
Kanamori, Hiroyuki
Wu, Jianzhong
Kawahigashi, Hiroyuki
Goto, Kazumi
description Cytoplasmic male sterility (CMS) is widely used to control pollination in the production of commercial F1 hybrid seed in sorghum. So far, 6 major fertility restorer genes, Rf1 to Rf6, have been reported in sorghum. Here, we fine-mapped the Rf5 locus on sorghum chromosome 5 using descendant populations of a ‘Nakei MS-3A’ × ‘JN43’ cross. The Rf5 locus was narrowed to a 140-kb region in BTx623 genome (161-kb in JN43) with 16 predicted genes, including 6 homologous to the rice fertility restorer Rf1 (PPR.1 to PPR.6). These 6 homologs have tandem pentatricopeptide repeat (PPR) motifs. Many Rf genes encode PPR proteins, which bind RNA transcripts and modulate gene expression at the RNA level. No PPR genes were detected at the Rf5 locus on the corresponding homologous chromosome of rice, foxtail millet, or maize, so this gene cluster may have originated by chromosome translocation and duplication after the divergence of sorghum from these species. Comparison of the sequences of these genes between fertile and CMS lines identified PPR.4 as the most plausible candidate gene for Rf5.
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So far, 6 major fertility restorer genes, Rf1 to Rf6, have been reported in sorghum. Here, we fine-mapped the Rf5 locus on sorghum chromosome 5 using descendant populations of a ‘Nakei MS-3A’ × ‘JN43’ cross. The Rf5 locus was narrowed to a 140-kb region in BTx623 genome (161-kb in JN43) with 16 predicted genes, including 6 homologous to the rice fertility restorer Rf1 (PPR.1 to PPR.6). These 6 homologs have tandem pentatricopeptide repeat (PPR) motifs. Many Rf genes encode PPR proteins, which bind RNA transcripts and modulate gene expression at the RNA level. No PPR genes were detected at the Rf5 locus on the corresponding homologous chromosome of rice, foxtail millet, or maize, so this gene cluster may have originated by chromosome translocation and duplication after the divergence of sorghum from these species. 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subjects Chromosome 5
Chromosome translocations
Chromosomes
Cytoplasmic male sterility
Divergence
Fertility
fine mapping
Gene duplication
Gene expression
Gene mapping
Genes
Genomes
Grasses
Homology
Loci
Male sterility
microsynteny
Pollination
Research Paper
Rf (fertility restorer) genes
Rf5
Ribonucleic acid
Rice
RNA
Sorghum
Translocation
title Fine mapping of Rf5 region for a sorghum fertility restorer gene and microsynteny analysis across grass species
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